会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 8. 发明申请
    • COMPLEX OF POLYSACCHARIDE AND DOUBLE-STRANDED RNA
    • 多糖和双链RNA的复合物
    • US20110111501A1
    • 2011-05-12
    • US12808504
    • 2008-12-18
    • Takanori KuboHideki OhbaKazuo SakuraiJusaku MinariAtsushi Uno
    • Takanori KuboHideki OhbaKazuo SakuraiJusaku MinariAtsushi Uno
    • C12N5/00C07H21/02
    • C12N15/111C12N2310/14C12N2310/3519C12N2320/32C12N2320/51
    • An object of the present invention is to provide novel double-stranded RNA having an RNA interference effect, in which the cellular uptake and the resistance to enzymatic degradation are improved, without reducing the RNA interference effect.The cellular uptake and the resistance to enzymatic degradation of a complex of a polysaccharide having a β-1,3-glucan skeleton and double-stranded RNA can be significantly improved while maintaining the RNA interference effect, by fulfilling the following conditions (i) to (iii): (i) the double-stranded RNA has a sense strand consisting of a base sequence complementary to a target sequence in a target gene and an antisense strand containing a base sequence complementary to the sense strand, and the double-stranded RNA can inhibit expression of the target gene; (ii) the double-stranded RNA has a single-stranded polydeoxyadenine bound directly or via a linker to the end of at least one of the sense strand and the antisense strand; and (iii) the polysaccharide and the single-stranded polydeoxyadenine form a complex.
    • 本发明的目的是提供具有RNA干扰效应的新颖的双链RNA,其中细胞摄取和对酶降解的抗性得到改善,而不降低RNA干扰效应。 通过满足以下条件,可以显着改善具有β-葡聚糖骨架和双链RNA的多糖的复合物的细胞摄取和抗降解,同时保持RNA干扰效应(i) 至(iii):(i)双链RNA具有由与靶基因中的靶序列互补的碱基序列的有义链和含有与有义链互补的碱基序列的反义链,双链 RNA可抑制靶基因的表达; (ii)双链RNA具有单链多脱氧腺嘌呤,其直接​​或经由接头连接至至少一个有义链和反义链的末端; 和(iii)多糖和单链多脱氧腺嘌呤形成复合物。