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1. 发明申请

US20090111176A1 Trehalose transporter gene and method of introducing trehalose into cells 有权
标题翻译：海藻糖转运蛋白基因和将海藻糖引入细胞的方法
公开(公告)号：US20090111176A1
公开(公告)日：2009-04-30
申请号：US12073457
申请日：2008-03-05
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
IPC分类号： C12N5/06 , C12N15/11 , C07K14/00 , C12N15/00
CPC分类号： C07K14/43563 , C07K14/43577
摘要： There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.
摘要翻译：提供海藻糖转运蛋白基因和通过使用该基因将海藻糖引入细胞的方法。在P. vanderplanki EST中搜索海藻糖转运蛋白基因的候选物，得到获得的命名为Tret1的cDNA。 Tret1编码具有12个跨膜结构的504个氨基酸的蛋白质。 Tret1表达由干燥应激诱导，在脂肪体中占优势。非洲爪蟾卵母细胞中TRET1的功能表达显示，转运活性对海藻糖是特异性的，独立于细胞外pH和电化学膜电位。根据海藻糖的浓度梯度，TRET1的转运方向是可逆的。 TRET1对于海藻糖的表观Km和Vmax是非常高的值。这些结果表明TRET1是一种促进的高容量海藻糖特异性转运蛋白。 Tret1在昆虫中广泛存在。此外，TRET1在包括脊椎动物以及昆虫的细胞中赋予海藻糖通透性。

2. 发明授权

US07892789B2 Method of increasing cell permeability to trehalose by recombinantly producing a trehalose transporter 有权
标题翻译：通过重组产生海藻糖转运蛋白增加海藻糖的细胞渗透性的方法
公开(公告)号：US07892789B2
公开(公告)日：2011-02-22
申请号：US12073457
申请日：2008-03-05
申请人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
发明人： Takahiro Kikawada , Takashi Okuda , Masahiko Watanabe , Ayako Saito , Yasushi Kanamori , Yuichi Nakahara
IPC分类号： C12P21/06
CPC分类号： C07K14/43563 , C07K14/43577
摘要： There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.
摘要翻译：提供海藻糖转运蛋白基因和通过使用该基因将海藻糖引入细胞的方法。在P. vanderplanki EST中搜索海藻糖转运蛋白基因的候选物，得到获得的命名为Tret1的cDNA。 Tret1编码具有12个跨膜结构的504个氨基酸的蛋白质。 Tret1表达由干燥应激诱导，在脂肪体中占优势。非洲爪蟾卵母细胞中TRET1的功能表达显示，转运活性对海藻糖是特异性的，独立于细胞外pH和电化学膜电位。根据海藻糖的浓度梯度，TRET1的转运方向是可逆的。 TRET1对于海藻糖的表观Km和Vmax是非常高的值。这些结果表明TRET1是一种促进的高容量海藻糖特异性转运蛋白。 Tret1在昆虫中广泛存在。此外，TRET1在包括脊椎动物以及昆虫的细胞中赋予海藻糖通透性。

3. 发明授权

US6090791A Method for inducing mucosal immunity 失效
标题翻译：诱导粘膜免疫的方法
公开(公告)号：US6090791A
公开(公告)日：2000-07-18
申请号：US123312
申请日：1998-07-28
申请人： Yukio Sato , Atsushi Irisawa , Ayako Saito , Reiji Kasukawa
发明人： Yukio Sato , Atsushi Irisawa , Ayako Saito , Reiji Kasukawa
IPC分类号： C12N15/09 , A61K31/00 , A61K31/70 , A61K39/00 , A61P37/08 , A61P43/00 , C07H21/04
CPC分类号： A61K31/711
摘要： By injecting plasmid DNAs or oligonucleotides with DNA sequence containing a 2 base sequence of unmethylated cytosine and guanine adjacent thereto into mamalian mucosal cells, mucosal immunity and CD4 positive T cells capable of producing interleukin 10 and IFN-.gamma. can be induced.
摘要翻译：通过将含有2个碱基序列的非甲基化胞嘧啶和鸟嘌呤的DNA序列注入质粒DNA或寡核苷酸，可以诱导粘膜免疫和能够产生白细胞介素10和IFN-γ的CD4阳性T细胞。

4. 发明授权

US06875726B2 Heat-sensitive recording material and process for production of the same 失效
标题翻译：热敏记录材料及其制造方法
公开(公告)号：US06875726B2
公开(公告)日：2005-04-05
申请号：US10067918
申请日：2002-02-08
申请人： Shigekazu Shuku , Ayako Saito
发明人： Shigekazu Shuku , Ayako Saito
IPC分类号： B41M5/42 , B41M5/40
CPC分类号： B41M5/42 , B41M2205/04 , B41M2205/40
摘要： Disclosed are a heat-sensitive recording material which comprises at least (a) a support, (b) a heat-sensitive recording layer formed on at least one side of the support and containing an electron-donating compound and an electron-accepting compound and (c) a protective layer, the protective layer being an outermost layer provided by being formed on a smooth-surfaced substrate and removing the smooth-surfaced substrate, and the protective layer surface having a distinctness of image (according to JIS K 7105-1981) of at least 75% (slit width 2 mm), as well as a process for preparing the heat-sensitive recording material.
摘要翻译：公开了一种热敏记录材料，其至少包括（a）支撑体，（b）在载体的至少一侧上形成的含有供电子化合物和电子接受性化合物的热敏记录层，（c）保护层，保护层是通过形成在光滑表面的基板上而去除光滑表面的基板而提供的最外层，以及具有明显的图像的保护层表面（根据JIS K 7105-1981）至少75％（狭缝宽度2mm），以及制备热敏记录材料的方法。

5. 发明授权

US06874489B2 Combustion chamber structure for internal combustion engine 失效
标题翻译：内燃机燃烧室结构
公开(公告)号：US06874489B2
公开(公告)日：2005-04-05
申请号：US10650126
申请日：2003-08-28
申请人： Akiyuki Yonekawa , Ayako Saito , Naohiro Ishikawa , Tohru Ohta , Seiji Mori
发明人： Akiyuki Yonekawa , Ayako Saito , Naohiro Ishikawa , Tohru Ohta , Seiji Mori
IPC分类号： F02B23/08 , F02F1/42 , F02F3/26 , F02B23/00
CPC分类号： F02F1/4214 , F02B23/08 , Y02T10/125
摘要： There are provided on an upper surface of a piston 13 which confronts to a combustion chamber two first squish areas A1 each containing a flat surface 13a extending along an outer circumferential portion the piston 13 in an arc-like fashion and two second squish areas A2 formed between valve recesses 13d, 13e of respective pairs of inlet and exhaust valves 19, 21 and each containing a protruding portion which protrudes upwardly.
摘要翻译：在活塞13的上表面上设有与燃烧室相对的两个第一挤压区域A1，每个第一挤压区域A1包含沿着圆弧状的活塞13的外周部分延伸的平坦表面13a和形成的两个第二挤压区域A2 在相应的一对入口和排气门19,21的阀槽13d，13e之间，并且每个包含向上突出的突出部分。

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