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1. 发明授权

US08389263B2 Spores for the stabilization and on-site application of bacterial whole-cell biosensing systems 失效
标题翻译：用于稳定和现场应用细菌全细胞生物传感系统的孢子
公开(公告)号：US08389263B2
公开(公告)日：2013-03-05
申请号：US12676302
申请日：2008-09-10
申请人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Amol Date
发明人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Amol Date
IPC分类号： C12N1/00 , C12N1/12 , C12N1/20 , C12N3/00
CPC分类号： C12N3/00 , C12Q1/02
摘要： The presently-disclosed subject matter is directed to biosensors comprising spore-forming bacterial cells and/or spores generated therefrom, a recognition unit within each spore-forming cell for binding an analyte of interest, and a reporter molecule within each spore-forming cell for detecting binding of the analyte of interest, wherein the reporter molecule generates a detectable signal upon binding of the analyte by the recognition element. The presently-disclosed subject matter further provides methods of using the biosensors and systems and kits including the biosensors.
摘要翻译：目前公开的主题涉及包含由其产生的形成孢子的细菌细胞和/或孢子的生物传感器，用于结合感兴趣分析物的每个孢子形成细胞内的识别单元和每个孢子形成细胞内的报道分子检测感兴趣的分析物的结合，其中报告分子在通过识别元件结合分析物时产生可检测的信号。目前公开的主题还提供使用生物传感器和包括生物传感器的系统和试剂盒的方法。

2. 发明申请

US20100304379A1 SPORES FOR THE STABILIZATION AND ON-SITE APPLICATION OF BACTERIAL WHOLE-CELL BIOSENSING SYSTEMS 失效
标题翻译：用于稳定和现场应用细菌生物传感系统的体育活动
公开(公告)号：US20100304379A1
公开(公告)日：2010-12-02
申请号：US12676302
申请日：2008-09-10
申请人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Amol Date
发明人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Amol Date
IPC分类号： G01N33/53 , C12N1/20 , C12N1/21 , C12N11/00 , C12Q1/02 , C12Q1/68
CPC分类号： C12N3/00 , C12Q1/02
摘要： The presently-disclosed subject matter is directed to biosensors comprising spore-forming bacterial cells and/or spores generated therefrom, a recognition unit within each spore-forming cell for binding an analyte of interest, and a reporter molecule within each spore-forming cell for detecting binding of the analyte of interest, wherein the reporter molecule generates a detectable signal upon binding of the analyte by the recognition element. The presently-disclosed subject matter further provides methods of using the biosensors and systems and kits including the biosensors.
摘要翻译：目前公开的主题涉及包含由其产生的形成孢子的细菌细胞和/或孢子的生物传感器，用于结合感兴趣分析物的每个孢子形成细胞内的识别单元和每个孢子形成细胞内的报道分子检测感兴趣的分析物的结合，其中报告分子在通过识别元件结合分析物时产生可检测的信号。目前公开的主题还提供使用生物传感器和包括生物传感器的系统和试剂盒的方法。

3. 发明申请

US20100291566A1 SYSTEMS AND METHODS FOR DIAGNOSIS AND MONITORING OF BACTERIA-RELATED CONDITIONS 失效
标题翻译：用于诊断和监测细菌相关条件的系统和方法
公开(公告)号：US20100291566A1
公开(公告)日：2010-11-18
申请号：US12676287
申请日：2008-09-10
申请人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Anjali Kumari Struss , Harohalli Shashidhar , Deborah R. Auer Flomenhoft , Nilesh Raut
发明人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Anjali Kumari Struss , Harohalli Shashidhar , Deborah R. Auer Flomenhoft , Nilesh Raut
IPC分类号： C12Q1/68 , C12Q1/04 , G01N33/53
CPC分类号： G01N33/6893 , G01N2800/065 , G01N2800/24 , G01N2800/26 , G01N2800/52
摘要： The presently-disclosed subject matter provides systems, methods, and kits for diagnosing and/or monitoring a bacteria-related condition of interest in a subject by providing a cell sensing system, each system containing a reporter molecule capable of detecting binding of a quorum sensing molecule and capable of generating a detectable signal.
摘要翻译：目前公开的主题提供用于通过提供细胞感测系统来诊断和/或监测受试者感兴趣的细菌相关状况的系统，方法和试剂盒，每个系统含有能够检测定量感测结合的报告分子分子并且能够产生可检测信号。

4. 发明授权

US08663927B2 Systems and methods for diagnosis and monitoring of bacteria-related conditions 失效
标题翻译：诊断和监测细菌相关病症的系统和方法
公开(公告)号：US08663927B2
公开(公告)日：2014-03-04
申请号：US12676287
申请日：2008-09-10
申请人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Anjali Kumari Struss , Harohalli Shashidhar , Deborah R. Auer Flomenhoft , Nilesh Raut
发明人： Sylvia Daunert , Sapna K. Deo , Patrizia Pasini , Anjali Kumari Struss , Harohalli Shashidhar , Deborah R. Auer Flomenhoft , Nilesh Raut
IPC分类号： G01N33/53
CPC分类号： G01N33/6893 , G01N2800/065 , G01N2800/24 , G01N2800/26 , G01N2800/52
摘要： The presently-disclosed subject matter provides systems, methods, and kits for diagnosing and/or monitoring a bacteria-related condition of interest in a subject by providing a cell sensing system, each system containing a reporter molecule capable of detecting binding of a quorum sensing molecule and capable of generating a detectable signal.
摘要翻译：目前公开的主题提供用于通过提供细胞感测系统来诊断和/或监测受试者感兴趣的细菌相关状况的系统，方法和试剂盒，每个系统含有能够检测定量感测结合的报告分子分子并且能够产生可检测信号。

5. 发明授权

US07659078B1 Method and kit for determination of prostacyclin in plasma 失效
标题翻译：血浆前列环素测定方法及试剂盒
公开(公告)号：US07659078B1
公开(公告)日：2010-02-09
申请号：US10620806
申请日：2003-07-17
申请人： Sylvia Daunert , Michael Poon , Urvee Desai , Sapna K. Deo
发明人： Sylvia Daunert , Michael Poon , Urvee Desai , Sapna K. Deo
IPC分类号： G01N33/53 , G01N33/537 , G01N33/543 , G01N33/88 , C12Q1/66 , C07K16/18 , C07K16/26
CPC分类号： G01N33/88 , G01N2800/12 , Y10S435/971 , Y10S435/975 , Y10S530/807
摘要： A solid-phase immunoassay for 6-keto-Prostaglandin F1α, the stable hydrolysis product of prostacyclin (Prostaglandin I2) is disclosed. Prostacyclin, a potent vasodilator with anti-platelet and anti-proliferative properties is an effective treatment for primary pulmonary hypertension and pulmonary arterial hypertension associated with scleroderma and scleroderma-like syndrome. Levels of 6-keto-Prostaglandin F1α can be directly correlated with levels of prostacyclin. Therefore, 6-keto-Prostaglandin F1α has become the indicator of choice to measure prostacyclin levels. The single step immunoassay for 6-keto-Prostaglandin F1α uses the bioluminescent protein, aequorin as a label. Analyte-label conjugates were constructed by linking the carboxyl group of 6-keto-Prostaglandin F1α and lysine residues of aequorin by chemical conjugation methods. The binding properties of 6-keto-Prostaglandin F1α towards its antibody and the bioluminescent properties of aequorin are retained in the conjugate. The concentration of 6-keto-Prostaglandin F1α after extraction from plasma shows good correlation with the concentration of 6-keto-Prostaglandin F1α obtained without prior extraction of the same plasma sample. The assay allows the measurement of 6-keto-Prostaglandin F1α directly in plasma without any pre-treatment of the samples, which results in a much simpler method with a faster assay time.
摘要翻译：公开了前列环素（前列腺素I2）的稳定水解产物6-酮 - 前列腺素F1alpha的固相免疫测定。前列环素，具有抗血小板和抗增殖特性的强效血管扩张剂是与硬皮病和硬皮病样综合征相关的原发性肺动脉高压和肺动脉高压的有效治疗方法。 6-keto-Prostaglandin F1alpha的水平可以与前列环素的水平直接相关。因此，6-keto-Prostaglandin F1alpha已经成为衡量前列环素水平的首选指标。 6-keto-Prostaglandin F1alpha的单步免疫测定使用生物发光蛋白，水母发光蛋白作为标记。通过化学共轭方法连接6-酮基前列腺素F1al的羧基和水母发光蛋白的赖氨酸残基，构建了分析标记物缀合物。 6-酮前列腺素F1alpha对其抗体的结合性质和水母发光蛋白的生物发光性质保留在缀合物中。从血浆中提取6-keto-Prostaglandin F1alpha的浓度与未经提取相同血浆样品的6-酮 - 前列腺素F1al的浓度呈现良好的相关性。该测定允许直接在血浆中测量6-酮基前列腺素F1alpha，而没有对样品进行任何预处理，这导致以更快的测定时间简单的方法。

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