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1. 发明授权

US09556440B2 Enhanced expression from the Pm promoter 有权
标题翻译：从Pm启动子增强表达
公开(公告)号：US09556440B2
公开(公告)日：2017-01-31
申请号：US12520139
申请日：2007-12-20
申请人： Rahmi Lale , Laila Berg , Ingrid Bakke , Trond Erik Vee Aune , Svein Valla
发明人： Rahmi Lale , Laila Berg , Ingrid Bakke , Trond Erik Vee Aune , Svein Valla
IPC分类号： C12N15/67 , C07K14/21 , C12N9/92
CPC分类号： C12N15/67 , C07K14/21 , C12N9/92
摘要： The present invention concerns a method of producing a desired gene product in a recombinant gene expression system, said method comprising expressing said gene from a Pm promoter-based expression system using at least two mutant elements selected from: (i) a mutant Pm promoter; (ii) a mutant mRNA leader; and (iii) a mutant XyIS; wherein said mutant elements each comprise one or more mutations which enhance expression of said desired gene. Particularly combinations of a mutant Pm promoter and a mutant mRNA leader are concerned. Isolated nucleic acid molecules, vectors, host cells, libraries, expression systems, methods of enhancing expression, obtaining nucleic acid molecules and identifying combination mutants which enhance expression, artificially constructed operons and their uses are also encompassed.
摘要翻译：本发明涉及在重组基因表达系统中产生所需基因产物的方法，所述方法包括使用选自以下的至少两种突变元件从基于Pm启动子的表达系统表达所述基因：（i）突变型Pm启动子; （ii）突变体mRNA引物; 和（iii）突变体XyIS; 其中所述突变体元件各自包含增强所述所需基因表达的一种或多种突变。涉及突变体Pm启动子和突变体mRNA引物的特别组合。还包括分离的核酸分子，载体，宿主细胞，文库，表达系统，增强表达的方法，获得核酸分子和鉴定增强表达的组合突变体，人造构建的操纵子及其用途。

2. 发明申请

US20100248972A1 Enhanced Expression Method 有权
标题翻译：增强表达法
公开(公告)号：US20100248972A1
公开(公告)日：2010-09-30
申请号：US12376312
申请日：2007-08-04
申请人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
发明人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
IPC分类号： C40B30/00 , C12Q1/68 , C12P19/34 , C12N15/63 , C40B40/08 , C07H21/02
CPC分类号： C12N15/70
摘要： The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening.
摘要翻译：本发明涉及产生所需异源基因产物的方法，其中所述异源基因产物由强启动子表达，所述方法包括使用突变型mRNA前导体表达所述基因，所述突变体mRNA引物包含一个或多个增强基因转录的突变。本发明还提供了突变体Pm mRNA前导序列，以及载体和包含前导序列的文库。包括获得mRNA突变体前导序列和鉴定mRNA突变体前导序列的方法，以及用于选择或鉴定mRNA前导突变体的载体及其用于筛选的用途。

3. 发明授权

US09388421B2 Enhanced expression method 有权
标题翻译：增强表达方法
公开(公告)号：US09388421B2
公开(公告)日：2016-07-12
申请号：US12376312
申请日：2007-08-03
申请人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
发明人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
IPC分类号： C12N15/70 , C07H21/04
CPC分类号： C12N15/70
摘要： The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening.
摘要翻译：本发明涉及产生所需异源基因产物的方法，其中所述异源基因产物由强启动子表达，所述方法包括使用突变型mRNA前导体表达所述基因，所述突变体mRNA引物包含一个或多个增强基因转录的突变。本发明还提供了突变体Pm mRNA前导序列，以及载体和包含前导序列的文库。包括获得mRNA突变体前导序列和鉴定mRNA突变体前导序列的方法，以及用于选择或鉴定mRNA前导突变体的载体及其用于筛选的用途。

4. 发明申请

US20100048426A1 Enhanced Expression from the Pm Promoter 有权
标题翻译：从Pm启动子增强表达
公开(公告)号：US20100048426A1
公开(公告)日：2010-02-25
申请号：US12520139
申请日：2007-12-20
申请人： Rahmi Lale , Laila Berg , Ingrid Bakke , Trond Erik Vee Aune , Svein Valla
发明人： Rahmi Lale , Laila Berg , Ingrid Bakke , Trond Erik Vee Aune , Svein Valla
IPC分类号： C07H21/00 , C12N15/63 , C12N5/10 , C40B40/08
CPC分类号： C12N15/67 , C07K14/21 , C12N9/92
摘要： The present invention concerns a method of producing a desired gene product in a recombinant gene expression system, said method comprising expressing said gene from a Pm promoter-based expression system using at least two mutant elements selected from: (i) a mutant Pm promoter; (ii) a mutant mRNA leader; and (iii) a mutant XyIS; wherein said mutant elements each comprise one or more mutations which enhance expression of said desired gene. Particularly combinations of a mutant Pm promoter and a mutant mRNA leader are concerned. Isolated nucleic acid molecules, vectors, host cells, libraries, expression systems, methods of enhancing expression, obtaining nucleic acid molecules and identifying combination mutants which enhance expression, artificially constructed operons and their uses are also encompassed.
摘要翻译：本发明涉及在重组基因表达系统中产生所需基因产物的方法，所述方法包括使用至少两种选自以下的突变元件从基于Pm启动子的表达系统表达所述基因：（i）突变型Pm启动子; （ii）突变体mRNA引物; 和（iii）突变体XyIS; 其中所述突变体元件各自包含增强所述所需基因表达的一种或多种突变。涉及突变体Pm启动子和突变体mRNA引物的特别组合。还包括分离的核酸分子，载体，宿主细胞，文库，表达系统，增强表达的方法，获得核酸分子和鉴定增强表达的组合突变体，人造构建的操纵子及其用途。

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