基本信息:
- 专利标题： Enhanced Expression Method
- 专利标题（中）：增强表达法
- 申请号：US12376312 申请日：2007-08-04
- 公开(公告)号：US20100248972A1 公开(公告)日：2010-09-30
- 发明人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
- 申请人： Svein Valla , Rahmi Lale , Laila Berg , Ingrid Bakke
- 专利权人： LEIF EIRIKSSON NYSKAPING AS CORPORATION
- 当前专利权人： LEIF EIRIKSSON NYSKAPING AS CORPORATION
- 优先权： GB0615556.8 20060804
- 国际申请： PCT/GB07/02951 WO 20070804
- 主分类号： C40B30/00
- IPC分类号： C40B30/00 ; C12Q1/68 ; C12P19/34 ; C12N15/63 ; C40B40/08 ; C07H21/02
摘要：
The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening.
摘要（中）：
本发明涉及产生所需异源基因产物的方法，其中所述异源基因产物由强启动子表达，所述方法包括使用突变型mRNA前导体表达所述基因，所述突变体mRNA引物包含一个或多个增强基因转录的突变。 本发明还提供了突变体Pm mRNA前导序列，以及载体和包含前导序列的文库。 包括获得mRNA突变体前导序列和鉴定mRNA突变体前导序列的方法，以及用于选择或鉴定mRNA前导突变体的载体及其用于筛选的用途。
公开/授权文献:
- US09388421B2 Enhanced expression method 公开/授权日：2016-07-12