专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08795966B2 Probe density self-considerations and elongation of complementary looped probes where probes are attached to a solid phase 有权
标题翻译：探针密度自我考虑和互补环状探针的伸长，其中探针连接到固相
公开(公告)号：US08795966B2
公开(公告)日：2014-08-05
申请号：US12708362
申请日：2010-02-18
申请人： Michael Seul , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
发明人： Michael Seul , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q1/6818 , C12Q1/6827 , C12Q2565/1015 , C12Q2537/143 , C12Q2533/101 , C12Q2535/125 , C12Q2525/301
摘要： In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps:adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.
摘要翻译：在溶液中进行的多重测定方法中，其中溶液含有核酸靶，并且其中使用几个不同类型的寡核苷酸探针，每个类型在被称为靶结合结构域的区域中具有不同序列，以检测核酸目标，所述测定方法包括通过一个或多个以下步骤增加寡核苷酸探针结合珠粒表面上的核酸靶的有效浓度的方法：调节测定条件以增加有效通过一种或多种以下方法可用于结合探针的靶的浓度：（i）在珠的表面上选择特定的探针密度; （ii）选择具有大于阈值的离子强度的溶液; （ii）选择小于阈值的大小的目标域; 或（iii）在目标的终端的指定接近范围内选择目标域。

2. 发明授权

US08486720B2 Arrays of magnetic particles 有权
标题翻译：磁性粒子阵列
公开(公告)号：US08486720B2
公开(公告)日：2013-07-16
申请号：US10910460
申请日：2004-08-03
申请人： Sukanta Banerjee , Michael Seul , Alice X. Li
发明人： Sukanta Banerjee , Michael Seul , Alice X. Li
IPC分类号： G01N33/553
CPC分类号： C12Q1/6813 , B01J19/0046 , B01J2219/00286 , B01J2219/00315 , B01J2219/00497 , B01J2219/005 , B01J2219/00527 , B01J2219/00545 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , C12Q1/6837 , C12Q1/6876 , G01N27/745 , G01N33/5434 , G01N33/54346 , G01N33/587 , G01N2035/00564 , G01N2035/00762 , G01N2446/20 , G01N2446/64 , Y10T436/11 , C12Q2565/513 , C12Q2563/143 , C12Q2563/107 , C12Q2563/155
摘要： The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
摘要翻译：本发明提供了一种通过产生磁性纳米颗粒和编码颗粒的新型亚文库从子文库生成新的编码磁性颗粒文库的方法。子库根据需要进行功能化在数组的形成中是有用的。本发明特别可用于进行多个（并行）测定以定性和/或定量分析样品中许多分析物分子的结合相互作用。

3. 发明申请

US20120220715A1 Method For Selecting Desired Level Of Dye Loading And Controlling Loading Of Polymer Microparticles 审中-公开
标题翻译：选择染料加载和控制聚合物微粒负载水平的方法
公开(公告)号：US20120220715A1
公开(公告)日：2012-08-30
申请号：US13417791
申请日：2012-03-12
申请人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
发明人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
IPC分类号： C08L25/08
CPC分类号： C08J3/212 , C08J3/215 , C08J3/22 , C08J2325/08
摘要： Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.
摘要翻译：通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒中的溶质量。该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

4. 发明授权

US07892854B2 Multianalyte molecular analysis using application-specific random particle arrays 有权
标题翻译：使用应用特异性随机粒子阵列进行多分析分析
公开(公告)号：US07892854B2
公开(公告)日：2011-02-22
申请号：US10204799
申请日：2001-06-21
申请人： Sukanta Banerjee , Michael Seul , Alice X. Li , Kairali Podual , Chiu W. Chau
发明人： Sukanta Banerjee , Michael Seul , Alice X. Li , Kairali Podual , Chiu W. Chau
IPC分类号： G01N33/553 , C12Q1/68
CPC分类号： C12Q1/6813 , B01J19/0046 , B01J2219/00286 , B01J2219/00315 , B01J2219/00497 , B01J2219/005 , B01J2219/00527 , B01J2219/00545 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , C12Q1/6837 , C12Q1/6876 , G01N27/745 , G01N33/5434 , G01N33/54346 , G01N33/587 , G01N2035/00564 , G01N2035/00762 , G01N2446/20 , G01N2446/64 , Y10T436/11 , C12Q2565/513 , C12Q2563/143 , C12Q2563/107 , C12Q2563/155
摘要： The present invention provides methods and apparatus for the application of a particle array in bioassay format to perform qualitative and/or quantitative molecular interaction analysis between two classes of molecules (an analyte and a binding agent). The methods and apparatus disclosed herein permit the determination of the presence or absence of association, the strength of association, and/or the rate of association and dissociation governing the binding interactions between the binding agents and the analyte molecules. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
摘要翻译：本发明提供了用于以生物测定形式应用颗粒阵列以在两类分子（分析物和结合剂）之间进行定性和/或定量分子相互作用分析的方法和装置。本文公开的方法和装置允许确定结合剂和分析物分子之间的结合相互作用的存在或不存在，缔合的强度，和/或缔合速率和解离速率。本发明特别可用于进行多个（并行）测定以定性和/或定量分析样品中许多分析物分子的结合相互作用。

5. 发明授权

US07255895B2 Method for controlling solute loading of polymer microparticles 有权
标题翻译：控制聚合物微粒溶质负载的方法
公开(公告)号：US07255895B2
公开(公告)日：2007-08-14
申请号：US10348165
申请日：2003-01-21
申请人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
发明人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
IPC分类号： B05D7/00
CPC分类号： C08J3/212 , C08J3/215 , C08J3/22 , C08J2325/08
摘要： Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.
摘要翻译：通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒的溶质量。该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

6. 发明申请

US20060035240A1 Optimization of gene expression analysis using immobilized capture probes 有权
公开(公告)号：US20060035240A1
公开(公告)日：2006-02-16
申请号：US10974036
申请日：2004-10-26
申请人： Michael Seul , Sukanta Banerjee , Jiacheng Yang , Tatiana Vener
发明人： Michael Seul , Sukanta Banerjee , Jiacheng Yang , Tatiana Vener
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , B01J2219/00459 , B01J2219/00576 , B01J2219/00608 , B01J2219/00648 , B01J2219/00722 , C12Q1/6809 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2565/501 , C12Q2565/507 , C12Q2533/101 , C12Q2565/514 , C12Q2565/519
摘要： Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target “engineering”, as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized (“grafted”) probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3′ terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.

7. 发明授权

US09436088B2 Un-supported polymeric film with embedded microbeads 有权
标题翻译：具有嵌入微珠的未负载的聚合物膜
公开(公告)号：US09436088B2
公开(公告)日：2016-09-06
申请号：US11761789
申请日：2007-06-12
申请人： Michael Seul , Sukanta Banerjee , Kairali Podual , Ye Hong
发明人： Michael Seul , Sukanta Banerjee , Kairali Podual , Ye Hong
IPC分类号： G01N33/553 , G03F7/16 , G03F7/004
CPC分类号： C08J3/075 , C08J3/28 , C08J2333/26 , C08J2425/06 , C12Q1/6837 , G01N33/5005 , G01N33/545 , G03F7/0047 , G03F7/164 , Y10T428/249952 , Y10T428/25
摘要： The present invention relates to polymer-bead composites having a single layer planar, crystalline assembly of encoded beads embedded in a hydrophilic polymeric matrix. The composite may be unattached to a solid support. The encoded beads have different biomolecules attached to their surfaces, and the encoding permits distinguishing beads having different biomolecules attached thereto. The present invention also relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites, including the AC electric field-mediated assembly of patterned, self-supporting organic (polymeric) films and organic-polymer-microparticle composites of tailored composition and morphology. The present invention also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, and micro-reactors, and miniaturized format for generation of multifunctional thin films.
摘要翻译：本发明涉及具有嵌入亲水性聚合物基质中的编码珠粒的单层平面结晶组合物的聚合物 - 珠复合物。复合材料可能不附着在固体支撑物上。编码的珠粒具有连接到其表面的不同生物分子，并且编码允许区分附着有不同生物分子的珠粒。本发明还涉及一种用于创建聚合物 - 微粒复合材料的功能组织的空间图案化组件的系统方法，包括图案化的自支撑有机（聚合物）膜和有机 - 聚合物 - 微粒的AC电场介导装配定制组合和形态的复合材料。本发明还涉及这种功能组件在材料科学和生物学中的应用。其他应用领域包括传感器，催化剂，膜和微反应器以及用于产生多功能薄膜的小型化形式。

8. 发明申请

US20100267578A1 PROBE DENSITY SELF-CONSIDERATIONS AND ELONGATION OF COMPLEMENTARY LOOPED PROBES WHERE PROBES ARE ATTACHED TO A SOLID PHASE 有权
标题翻译：探索密度自我考虑和延伸的复杂循环探测器，其中探测器连接到固相
公开(公告)号：US20100267578A1
公开(公告)日：2010-10-21
申请号：US12708362
申请日：2010-02-18
申请人： MICHAEL SEUL , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
发明人： MICHAEL SEUL , Yi Zhang , Sukanta Banerjee , Jiacheng Yang , Chiu Chau
IPC分类号： C40B30/04
CPC分类号： C12Q1/6837 , C12Q1/6818 , C12Q1/6827 , C12Q2565/1015 , C12Q2537/143 , C12Q2533/101 , C12Q2535/125 , C12Q2525/301
摘要： In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps:adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.
摘要翻译：在溶液中进行的多重测定方法中，其中溶液含有核酸靶，并且其中使用几个不同类型的寡核苷酸探针，每个类型在被称为靶结合结构域的区域中具有不同序列，以检测核酸目标，所述测定方法包括通过一个或多个以下步骤增加寡核苷酸探针结合珠粒表面上的核酸靶的有效浓度的方法：调节测定条件以增加有效通过一种或多种以下方法可用于结合探针的靶的浓度：（i）在珠的表面上选择特定的探针密度; （ii）选择具有大于阈值的离子强度的溶液; （ii）选择小于阈值的大小的目标域; 或（iii）在目标的终端的指定接近范围内选择目标域。

9. 发明授权

US07498054B2 Method for controlling solute loading of polymer microparticles 有权
标题翻译：控制聚合物微粒溶质负载的方法
公开(公告)号：US07498054B2
公开(公告)日：2009-03-03
申请号：US11179135
申请日：2005-07-12
申请人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
发明人： Sukanta Banerjee , Cecilia Georgescu , Michael Seul
IPC分类号： B05D7/00 , C09D11/00
CPC分类号： C08J3/212 , C08J3/215 , C08J3/22 , C08J2325/08
摘要： Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.
摘要翻译：通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒的溶质量。该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

10. 发明申请

US20080123089A1 Quality control method for making a biochip displaying an encoded bead array 有权
标题翻译：用于制造显示编码珠阵列的生物芯片的质量控制方法
公开(公告)号：US20080123089A1
公开(公告)日：2008-05-29
申请号：US11874355
申请日：2007-10-18
申请人： Michael Seul , Chiu Wo Chau , Hui Huang , Sukanta Banerjee , Jiacheng Yang , Ye Hong
发明人： Michael Seul , Chiu Wo Chau , Hui Huang , Sukanta Banerjee , Jiacheng Yang , Ye Hong
IPC分类号： G01N21/00
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00545 , B01J2219/00648 , B01J2219/00662 , G01N33/54306 , C12Q2563/149
摘要： This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.
摘要翻译：本发明提供高单位密度的微粒阵列和组装这种阵列的方法。阵列中的微粒可以用给定靶分析物特异性的化学或生物实体来官能化。本发明的高单位密度阵列形成在芯片上，芯片可以根据本文所述的方法组合以形成多芯片阵列。本发明的芯片和/或多芯片阵列可用于化学和生物测定。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式