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1. 发明授权

US09017973B2 Methods and compositions for incorporating nucleotides 有权
标题翻译：掺入核苷酸的方法和组合物
公开(公告)号：US09017973B2
公开(公告)日：2015-04-28
申请号：US13305415
申请日：2011-11-28
申请人： Steven Gordon , Jerzy Olejnik
发明人： Steven Gordon , Jerzy Olejnik
IPC分类号： C12P19/34 , C12Q1/68 , G01N21/05 , G01N21/64 , C07H21/04 , C07H19/04 , G01N21/03
CPC分类号： C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
摘要翻译：本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。本发明的方法包括校正核苷酸测序期间遇到的一种或多种现象，例如通过合成方法进行测序。这些现象包括但不限于序列引导，序列滞后，光谱串扰以及由照明和/或滤波器响应变化引起的噪声。

2. 发明申请

US20100323350A1 Methods And Devices For Sequencing Nucleic Acids In Smaller Batches 有权
标题翻译：在较小批次中测序核酸的方法和装置
公开(公告)号：US20100323350A1
公开(公告)日：2010-12-23
申请号：US12719469
申请日：2010-03-08
申请人： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
发明人： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： G01N27/44791 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487 , C12Q1/6869 , G01N27/44726
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
摘要翻译：本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。为了提供更大的灵活性和效率，采用多个较小的流动池，每个具有相对小的要被成像的面积。

3. 发明授权

US08481259B2 Methods and devices for sequencing nucleic acids in smaller batches 有权
公开(公告)号：US08481259B2
公开(公告)日：2013-07-09
申请号：US12719469
申请日：2010-03-08
申请人： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
发明人： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： G01N27/44791 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487 , C12Q1/6869 , G01N27/44726
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

4. 发明申请

US20130137091A1 Methods And Compositions For Incorporating Nucleotides 有权
公开(公告)号：US20130137091A1
公开(公告)日：2013-05-30
申请号：US13305415
申请日：2011-11-28
申请人： Steven Gordon , Jerzy Olejnik
发明人： Steven Gordon , Jerzy Olejnik
IPC分类号： G01N21/64
CPC分类号： C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

5. 发明授权

US09309569B2 Methods and compositions for sequencing nucleic acid using charge 有权
标题翻译：使用电荷测序核酸的方法和组合物
公开(公告)号：US09309569B2
公开(公告)日：2016-04-12
申请号：US13212910
申请日：2011-08-18
申请人： Steven Gordon , Jerzy Olejnik
发明人： Steven Gordon , Jerzy Olejnik
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C07H19/04
CPC分类号： C12Q1/6874 , C12Q1/6869 , C12Q2525/117 , C12Q2565/30
摘要： The invention provides methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, including sequences with one or more homopolymer regions. The methods of the invention include improvements so as to accurately identify sequences, including the difficult homopolymer sequences that are encountered during nucleotide sequencing, such as pyrosequencing.
摘要翻译：本发明提供了用于确定核苷酸序列中核酸的同一性的方法和组合物以及系统，包括具有一个或多个均聚物区域的序列。本发明的方法包括改进以便准确地鉴定序列，包括在核苷酸测序期间遇到的困难的均聚物序列，如焦磷酸测序。

6. 发明授权

US08288098B2 Bioreactive agents 失效
标题翻译：生物反应剂
公开(公告)号：US08288098B2
公开(公告)日：2012-10-16
申请号：US13283209
申请日：2011-10-27
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.
摘要翻译：本发明涉及用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白质。试剂包含与光反应性部分结合的可检测部分。缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。缀合物暴露于放射线以功能形式释放靶，完全不变。使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。

7. 发明授权

US08278045B2 Methods for the detection, analysis and isolation of nascent proteins 有权
标题翻译：新生蛋白的检测，分析和分离方法
公开(公告)号：US08278045B2
公开(公告)日：2012-10-02
申请号：US13023725
申请日：2011-02-09
申请人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sadanand Gite , Jerzy Olejnik
IPC分类号： C12Q1/68
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
摘要翻译：本发明涉及促进在细胞或无细胞翻译系统中翻译的新生蛋白的检测和分析的非放射性标记物。可以快速有效地检测，分离和分析含有这些标记物的蛋白质，而不会与放射性试剂相关的处理和处置问题。优选的标记是二吡咯亚甲基二氟化物（4,4-二氟-4-硼酸-3a，4a-二氮杂 - 顺 - 并苯并三嗪）染料。

8. 发明申请

US20110129878A1 BIOREACTIVE AGENTS 审中-公开
标题翻译：生物制剂
公开(公告)号：US20110129878A1
公开(公告)日：2011-06-02
申请号：US12944425
申请日：2010-11-11
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12P19/34 , C07H21/00 , C07H21/04 , C07H21/02
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
摘要翻译：本发明涉及可用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白。试剂包含与光反应性部分结合的可检测部分。缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。缀合物暴露于放射线以功能形式释放靶，完全不变。使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。有用的组合物包括生物制剂如核酸，蛋白质，脂质和细胞因子。缀合物也可用于监测药物组合物在体内和诊断，治疗和预防目的中的途径和半衰期。本发明还涉及可用于检测疾病，病症和物质复杂背景中的几乎任何单一物质的试剂和缀合物的试剂盒。

9. 发明申请

US20100196988A1 Bioreactive Agents 审中-公开
标题翻译：生物反应剂
公开(公告)号：US20100196988A1
公开(公告)日：2010-08-05
申请号：US12629234
申请日：2009-12-02
申请人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
发明人： Kenneth J. Rothschild , Sanjay M. Sonar , Jerzy Olejnik
IPC分类号： C12N9/10 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates; label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
摘要翻译：本发明涉及可用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白。试剂包含与光反应性部分结合的可检测部分。缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。缀合物暴露于放射线以功能形式释放靶，完全不变。使用可光致发光的分子前体作为共轭物; 标签可以并入大分子，新生大分子被分离，标签完全去除。本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。有用的组合物包括生物制剂如核酸，蛋白质，脂质和细胞因子。缀合物也可用于监测药物组合物在体内和诊断，治疗和预防目的中的途径和半衰期。本发明还涉及可用于检测疾病，病症和物质复杂背景中的几乎任何单一物质的试剂和缀合物的试剂盒。

10. 发明授权

US07547530B2 Nucleotide compositions comprising photocleavable markers and methods of preparation thereof 有权
标题翻译：包含可光切割标记的核苷酸组合物及其制备方法
公开(公告)号：US07547530B2
公开(公告)日：2009-06-16
申请号：US11351996
申请日：2006-02-09
申请人： Jerzy Olejnik , Edyta Krzymanska-Olejnik , Kenneth J. Rothschild
发明人： Jerzy Olejnik , Edyta Krzymanska-Olejnik , Kenneth J. Rothschild
IPC分类号： C12P19/34 , C12Q1/68 , C07H19/04 , C07H21/02
CPC分类号： A61K49/0032 , A61K49/0021 , A61K49/0052 , C07H19/00 , C07H19/04 , C07H21/02 , C07H21/04
摘要： Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
摘要翻译：描述了用于核酸测序的标记核苷酸和多核苷酸。描述了制备可光切割标记核苷酸和可光切割标记 - 多核苷酸缀合物的方法。可将这种可光切割的标记核苷酸掺入核酸中，以产生可光切割的标记 - 多核苷酸缀合物。

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