专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20110124053A1 POLYMERASE INCORPORATION OF NON-STANDARD NUCLEOTIDES 有权
标题翻译：聚合物纳入非标准核素
公开(公告)号：US20110124053A1
公开(公告)日：2011-05-26
申请号：US12999138
申请日：2009-06-16
申请人： Steven Albert Benner , Fei Chen , Zunyi Yang
发明人： Steven Albert Benner , Fei Chen , Zunyi Yang
IPC分类号： C12P19/34
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/686 , C12Q2521/101 , C12Q2525/117 , C12Q2525/191
摘要： The disclosed invention teaches processes to amplify oligonucleotides by contacting templates and primers with DNA polymerases and triphosphates of non-standard nucleotides, which form nucleobase pairs fitting the standard Watson-Crick geometry, but joined by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. Thus, this invention relates to nucleotide analogs and their derivatives that, when incorporated into DNA and RNA, expand the number of replicatable nucleotides beyond the four found in standard DNA and RNA. The invention further relates to polymerases that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives, and more specifically, polymerases and non-standard nucleoside triphosphates that support the polymerase chain reaction (PCR), including PCR where the products contain more than one non-standard nucleotide unit. Examples are provides that show this process using 6-amino-5-nitro-3-(1′-beta-D-2′-deoxyribofuranosyl)-2(1H)-pyridone to implement the non-standard “small” donor-donor-acceptor (pyDDA) hydrogen bonding pattern, and 2-amino-8-(r-beta-D-2′-deoxyribofuranosyl)-imidazo[1,2-α]-1,3,5-triazin-4(8H)-one to implement the “large” acceptor-acceptor-donor (puADD) pattern.
摘要翻译：所公开的发明教导了通过使模板和引物与DNA聚合酶和非标准核苷酸的三磷酸接触来扩增寡核苷酸的过程，其形成适合标准Watson-Crick几何形状的核碱基对，但是通过与加入标准A： T和G：C对。因此，本发明涉及核苷酸类似物及其衍生物，其在掺入DNA和RNA时扩大超出标准DNA和RNA中发现的四种可重复核苷酸的数量。本发明还涉及使用相应的三磷酸衍生物，更具体地，支持聚合酶链式反应（PCR）的聚合酶和非标准核苷三磷酸（包括产物含有的PCR）的寡核苷酸产物，将这些非标准核苷酸类似物结合到聚合酶中的聚合酶多个非标准核苷酸单位。提供了使用6-氨基-5-硝基-3-（1'-β-D-2'-脱氧三呋喃糖基）-2（1H） - 吡啶酮来实施非标准“小”供体 - 供体 - 受体（pyDDA）氢键图案和2-氨基-8-（r-β-D-2'-脱氧三呋喃糖基） - 咪唑并[1,2-α] -1,3,5-三嗪-4（8H） - 实施“大”受体 - 受体 - 供体（puADD）模式。

2. 发明授权

US08034923B1 Reagents for reversibly terminating primer extension 有权
标题翻译：可逆终止引物延伸的试剂
公开(公告)号：US08034923B1
公开(公告)日：2011-10-11
申请号：US12383776
申请日：2009-03-27
申请人： Steven Albert Benner , Daniel Hutter , Nicole Aurora Leal , Fei Chen
发明人： Steven Albert Benner , Daniel Hutter , Nicole Aurora Leal , Fei Chen
IPC分类号： C07H21/02 , C07H21/04 , C07H19/04 , C07H19/048 , C07H19/067 , C07H19/073 , C07H19/167 , C07H19/173
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00
摘要： Processes are disclosed that use 3′-reversibly terminated nucleoside triphosphates to analyze DNA for purposes other than sequencing using cyclic reversible termination. These processes are based on the unexpected ability of terminal transferase to accept these triphosphates as substrates, the unexpected ability of polymerases to add reversibly and irreversibly terminated triphosphates in competition with each other, the development of cleavage conditions to remove the terminating group rapidly, in high yield, and without substantial damage to the terminated oligonucleotide product, and the ability of reversibly terminated primer extension products to capture groups. The presently preferred embodiments of the disclosed processes use a triphosphate having its 3′-OH group blocked as a 3′-ONH2 group, which can be removed in buffered NaNO2 and use variants of Taq DNA polymerase, including one that has a replacement (L616A).
摘要翻译：公开了使用3'-可逆终止的核苷三磷酸以分析DNA作为除了使用循环可逆终止的测序之外的目的的方法。这些过程是基于终端转移酶接受这些三磷酸盐作为底物的意想不到的能力，聚合酶意想不到的能力相互添加可逆地和不可逆地终止的三磷酸酯，发展切割条件以迅速去除终止组，高产生并且对终止的寡核苷酸产物没有实质损害，以及可逆终止的引物延伸产物捕获基团的能力。所公开方法的目前优选的实施方案使用其3'-OH基团被阻断作为3'-ONH2基团的三磷酸酯，其可以在缓冲的NaNO 2中除去，并且使用Taq DNA聚合酶的变体，包括具有替换的变体（L616A ）。

3. 发明授权

US08871469B1 Self-avoiding molecular recognition systems in DNA priming 有权
标题翻译：自我回避分子识别系统在DNA启动中
公开(公告)号：US08871469B1
公开(公告)日：2014-10-28
申请号：US12229159
申请日：2008-08-20
申请人： Steven Albert Benner , Shuichi Hoshika , Fei Chen
发明人： Steven Albert Benner , Shuichi Hoshika , Fei Chen
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/6853 , C07H21/04 , C12Q1/6848 , C12Q1/6876 , C12Q2525/117 , C12Q2600/16 , C12Q2531/113 , C12Q2527/107 , C12Q2525/185 , C12Q2537/143 , C12Q2525/101
摘要： This invention concerns self-avoiding molecular recognition systems (SAMRS), compositions that bind to natural DNA and RNA, but do not bind to compositions at sites that incorporate other SAMRS components, and processes dependent on them. Their utility is shown by discoveries that DNA polymerases accept these compositions as primers and templates, where standard triphosphates are added to primers containing SAMRS components, and added opposite to SAMRS components in the template. A critical mass of data are provided in 16 examples to provide first-generation heuristic rules to permit design of SAMRS sequences can be used as primers and templates that are accepted by DNA polymerases. The presently preferred primers are at least 12 nucleotide units in length, and more preferably between 15 and 30 nucleotides in length. Also preferred are chimeric primers that have standard nucleotides in their 5′-segments, and SAMRS nucleotides in their 3′-segments, and in multiplexed priming.
摘要翻译：本发明涉及自回避分子识别系统（SAMRS），与天然DNA和RNA结合但不结合其他SAMRS组分的位点的组合物以及依赖于它们的方法的组合物。通过发现DNA聚合酶接受这些组合物作为引物和模板，其中将标准三磷酸加入到含有SAMRS组分的引物中，并与模板中的SAMRS组分相反地添加，发现其实用性。在16个例子中提供了大量数据，以提供第一代启发式规则，以允许SAMRS序列的设计可以用作DNA聚合酶所接受的引物和模板。目前优选的引物长度为至少12个核苷酸单位，更优选长度为15至30个核苷酸。还优选的是在其5'-节段中具有标准核苷酸的嵌合引物，以及它们的3'-区段中的SAMRS核苷酸以及多重引发。

4. 发明授权

US08212020B2 Reagents for reversibly terminating primer extension 有权
标题翻译：可逆终止引物延伸的试剂
公开(公告)号：US08212020B2
公开(公告)日：2012-07-03
申请号：US12383306
申请日：2009-03-23
申请人： Steven Albert Benner , Daniel Hutter , Nicole Aurora Leal , Fei Chen
发明人： Steven Albert Benner , Daniel Hutter , Nicole Aurora Leal , Fei Chen
IPC分类号： C07H17/02 , C07H19/04 , C07H19/10 , C07H19/20 , C07H21/04
CPC分类号： C07H19/10 , C07H19/073 , C07H19/14 , C07H19/173 , C07H23/00
摘要： This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions of matter that comprise triphosphates of modified 2′-deoxynucleosides and oligonucleotides that are formed when these are appended to the 3′-end of a primer, wherein said modifications comprise NH2 moiety attached to their 3′-hydroxyl group and a fluorescent species in a form of a tag affixed to the nucleobase via a linker that can be cleaved. Such compositions and their associated processes enable and improve the sequencing of oligonucleotides using a strategy of cyclic reversible termination, as outlined in U.S. Pat. No. 6,664,079. Most specifically, the invention concerns compositions of matter that are 5′-triphosphates of ribo- and 2′-deoxyribonucleosides carrying detectable tags and oligonucleotides that might be derived from them. The invention also concerns processes wherein a DNA polymerase, RNA polymerase, or reverse transcriptase synthesizes said oligonucleotides via addition of said triphosphates to a primer.
摘要翻译：本发明涉及核酸化学领域，更具体地涉及包含修饰的2'-脱氧核苷的三磷酸的物质组合物领域和当它们附着于引物的3'-末端时形成的寡核苷酸，其中所述修饰包括连接到它们的3'-羟基上的NH 2部分和通过能够被切割的接头固定在核碱基上的标签形式的荧光物质。这样的组合物及其相关方法使用循环可逆终止的策略实现和改进了寡核苷酸的测序，如美国专利No. 6,664,079。最具体地，本发明涉及携带检测标签的核糖核酸和2'-脱氧核糖核苷的5'-三磷酸的物质组合物，所述可检测标签和寡核苷酸可能来源于它们。本发明还涉及其中DNA聚合酶，RNA聚合酶或逆转录酶通过向引物中加入所述三磷酸合成所述寡核苷酸的方法。

5. 外观设计

USD861664S1 Mobile phone case 有权
公开(公告)号：USD861664S1
公开(公告)日：2019-10-01
申请号：US29636186
申请日：2018-02-07
申请人： Fei Chen
设计人： Fei Chen

6. 发明申请

US20150168410A1 BIOMARKERS FOR COLORECTAL CANCER DIAGNOSIS AND PREDICTION 审中-公开
标题翻译：生物标志物进行彩色癌症诊断和预测
公开(公告)号：US20150168410A1
公开(公告)日：2015-06-18
申请号：US14371088
申请日：2012-01-09
申请人： Tao Wang , Xiangyun Qu , Fei Chen
发明人： Tao Wang , Xiangyun Qu , Fei Chen
IPC分类号： G01N33/574 , C07K16/38 , C12Q1/68
CPC分类号： G01N33/57419 , C07K14/8139 , C07K16/38 , C12Q1/6886 , C12Q2600/158 , G01N2333/8139
摘要： In this invention, we disclosed and documented the applications of CST4 gene, mRNA of CST4, cDNA of CST4 splices, the corresponding amplicon of CST4-specific primers, cystatin S protein coded by CST4 gene and epitope peptide of CST4 in the diagnosis and prediction of colorectal cancer, and the detection method and testing kits of the above. This invention shall be applied in the diagnosis, dynamic monitoring and progression prediction of colorectal cancer. Methods mentioned in this invention features high sensitivity and reliability, which was verified by large-scale trial.
摘要翻译：在本发明中，我们公开并记录了CST4基因的mRNA，CST4的mRNA，CST4剪接的cDNA，CST4特异性引物的相应扩增子，CST4基因编码的半胱氨酸蛋白酶抑制蛋白S蛋白和CST4的表位肽在诊断和预测中的应用结肠直肠癌，以及上述的检测方法和检测试剂盒。本发明应用于结肠直肠癌的诊断，动态监测和进展预测。本发明提出的方法具有高灵敏度和可靠性，经大规模试验验证。

7. 发明授权

US09052513B2 Laser scan unit for an imaging device 有权
标题翻译：用于成像设备的激光扫描单元
公开(公告)号：US09052513B2
公开(公告)日：2015-06-09
申请号：US13464942
申请日：2012-05-04
申请人： Fei Chen , Craig Eric Hadady , Christopher Dane Jones , Simarpreet Singh Rattan
发明人： Fei Chen , Craig Eric Hadady , Christopher Dane Jones , Simarpreet Singh Rattan
IPC分类号： B41J27/00 , G03G21/20 , G02B26/10 , G03G15/04
CPC分类号： G02B26/10 , G03G15/04072 , G03G2215/0145
摘要： A system and method for reducing scan line jitter caused by facet cut variation in scan systems employing a plurality of laser sources and a sensor for generating the timing for the laser sources. The system includes a controller for determining a unique time delay for each facet of the rotating mirror, and controlling the laser sources so that video provided by each laser source is delayed in a scan line by the unique time delay corresponding to the facet of the rotating mirror used in creating the scan line.
摘要翻译：一种用于减少由采用多个激光源的扫描系统中的小切割变化引起的扫描线抖动的系统和方法，以及用于产生激光源的定时的传感器。该系统包括用于确定旋转镜的每个面的唯一时间延迟的控制器，并且控制激光源，使得由每个激光源提供的视频在扫描线中被延迟与旋转的面相对应的唯一时间延迟用于创建扫描线的镜像。

8. 发明申请

US20140038115A1 Dense/Dilute Pulverized Coal Separator Structure of Single-fireball Octagonal Direct-flow Burner 审中-公开
标题翻译：密封/稀释粉煤分离器单火焰八边形直流燃烧器结构
公开(公告)号：US20140038115A1
公开(公告)日：2014-02-06
申请号：US13808121
申请日：2012-02-16
申请人： Fei Chen , Jianwen Zhang , Kun Xiao , Jiangtao Li
发明人： Fei Chen , Jianwen Zhang , Kun Xiao , Jiangtao Li
IPC分类号： F23C5/28 , F22B21/34 , F23D1/00 , F23K1/00
CPC分类号： F23C5/28 , F22B21/34 , F23C5/32 , F23D1/00 , F23D1/005 , F23K1/00 , F23L9/02
摘要： The invention discloses a dense/dilute pulverized coal separator structure of a single-fireball octagonal direct-flow burner, of which a boiler body is provided with eight burner groups, each water cooled wall is provided with two burner groups respectively, each of the burner groups comprises multiple nozzles toward the same burner, and center lines of all nozzles on the eight burner groups form an imaginary tangent circle in a furnace along the same tangential direction. In the dense/dilute pulverized coal separator structure, eight burner groups are arranged on four water cooled walls of the boiler, thus increasing pulverized coal concentration of a pulverized rich coal area, allowing wall heat load qHr of a lower burner area to be higher, allowing burning temperature of the area to meet requirements for anthracite burning stability, shortening distance of jet flow from a nozzle outlet to downstream adjacent air flow, being capable of using lower primary pulverized coal air flow velocity, enhancing heat flow intensity at the nozzle outlet, improving convection and radiation heat transfer capacity, and ensuring timely ignition of pulverized anthracite air flow and stable burning of the boiler at low load without oil.
摘要翻译：本发明公开了一种单火焰八边形直流燃烧器的致密/稀释粉煤分离器结构，其锅炉体设有八个燃烧器组，每个水冷壁分别设有两个燃烧器组，每个燃烧器组包括朝向相同燃烧器的多个喷嘴，并且八个燃烧器组上的所有喷嘴的中心线在炉中沿相同切向方向形成假想切圆。在致密/稀释的粉煤分离器结构中，锅炉的四个水冷壁上布置了八个燃烧器组，从而提高了富煤煤层的粉煤浓度，使下燃烧器面积的壁热负荷qHr更高，使得该区域的燃烧温度满足无烟煤燃烧稳定性的要求，缩短从喷嘴出口到下游相邻气流的喷流的距离，能够使用较低的初级粉煤气流速度，增强喷嘴出口处的热流强度，提高对流和辐射传热能力，确保煤粉无烟煤气流的及时点火，锅炉低负荷无油燃烧。

9. 发明授权

US08247444B2 Anti-tumor effect of dimeric phthalide compound 有权
标题翻译：二聚苯酞化合物的抗肿瘤作用
公开(公告)号：US08247444B2
公开(公告)日：2012-08-21
申请号：US12523792
申请日：2007-01-19
申请人： Fei Chen , Tao Wang , Yifeng Wu
发明人： Fei Chen , Tao Wang , Yifeng Wu
IPC分类号： A01N43/12 , A01N43/26 , A01N43/08 , A61K31/34 , A61K31/335
CPC分类号： A61K31/365 , C07D307/88 , C07D493/08 , C07D493/10
摘要： The present invention provides for the use of a dimeric phthalide compound, the dimeric phthalide compound has broad anti-tumor activity, can directly inhibit the proliferation of tumor cells and induce cell death, but also indirectly inhibit the development of tumors by suppressing neovascularization. And further, the dimeric phthalide compound can be used alone or in combination to treat cancer, as well as in combination with chemotherapeutics to increase the efficacy of chemotherapeutics and decrease the toxicity of chemotherapeutics.
摘要翻译：本发明提供二聚苯酞化合物的用途，二苯醚膦化合物具有广泛的抗肿瘤活性，可直接抑制肿瘤细胞的增殖并诱导细胞死亡，而且通过抑制新生血管形成间接抑制肿瘤的发生。此外，二聚苯酞化合物可以单独使用或组合使用以治疗癌症，以及与化学治疗剂组合以增加化学治疗剂的功效并降低化学治疗剂的毒性。

10. 发明申请

US20100242091A1 NETWORK VIDEO MESSAGING 有权
标题翻译：网络视频消息传递
公开(公告)号：US20100242091A1
公开(公告)日：2010-09-23
申请号：US12408909
申请日：2009-03-23
申请人： Zhangwei Xu , Martin Hall , Mark McNulty , Guruprakash Rao , Xiaofeng Gao , Fei Chen , Ricardo Lopez-Barquilla , Martin Holladay
发明人： Zhangwei Xu , Martin Hall , Mark McNulty , Guruprakash Rao , Xiaofeng Gao , Fei Chen , Ricardo Lopez-Barquilla , Martin Holladay
IPC分类号： G06F15/16 , G06F17/30 , G06F21/00 , H04L9/32
CPC分类号： G06F21/10 , H04L29/00 , H04L51/08 , H04L51/10 , H04L51/24 , H04L63/08 , H04L63/126 , H04L67/06 , H04L2463/101
摘要： Embodiments related to network video messaging are disclosed. One disclosed embodiment provides a method that comprises receiving a video message from a client application of a source client; associating a navigation link with the video message; transmitting a notification message to the recipient client including the navigation link; receiving a retrieval request from the recipient client to access the video content via the navigation link; and transmitting the video content to the recipient client responsive to receiving the retrieval request by providing a persistent download of the video content from the storage server if the download condition indicates that a persistent download of the video content is permissible; and providing a transient download of the video content to the recipient client while prohibiting a persistent download of the video content if the download condition indicates that a persistent download of the video content is not permissible.
摘要翻译：公开了与网络视频消息相关的实施例。一个公开的实施例提供了一种方法，包括从源客户端的客户端应用接收视频消息; 将导航链接与视频消息相关联; 向包括导航链路的接收方客户端发送通知消息; 从所述接收方客户接收检索请求，经由所述导航链接访问所述视频内容; 以及如果所述下载条件指示所述视频内容的持续下载是允许的，则通过从所述存储服务器提供所述视频内容的持续下载来响应于接收到所述检索请求而将所述视频内容发送到所述接收者客户端; 以及如果所述下载条件指示所述视频内容的持续下载是不允许的，则向所述接收者客户端提供所述视频内容的暂时下载，同时禁止所述视频内容的持续下载。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式