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1. 发明授权

US08024697B2 Various methods and apparatuses for estimating characteristics of an electronic systems design 有权
标题翻译：用于估计电子系统设计特性的各种方法和装置
公开(公告)号：US08024697B2
公开(公告)日：2011-09-20
申请号：US12729509
申请日：2010-03-23
申请人： Stephen Hamilton , Ian Andrew Swarbrick , Scott Carlton Evans , Wolf-Dietrich Weber , Jay S. Tomlinson
发明人： Stephen Hamilton , Ian Andrew Swarbrick , Scott Carlton Evans , Wolf-Dietrich Weber , Jay S. Tomlinson
IPC分类号： G06F17/50 , G06F9/455 , G06F11/22
CPC分类号： G06F17/5045 , G06F17/50 , G06F2217/78 , G06F2217/84
摘要： Methods and apparatuses are described for an Intellectual Property (IP) Generator for estimating timing, area, and power characteristics of an electronic system design. The IP Generator receives a user-supplied file having data describing a configuration of an IP design having multiple levels of hierarchy. The IP Generator also receives user-supplied technology parameters and data-flow information. The IP generator correlates estimated timing, area, and power characteristics for each IP sub component based on the user supplied technology parameters, data-flow information and configuration parameters. The IP generator reports the timing, area, and power estimates to a user via a graphic user interface prior to a transformation of a Register Transfer Level (RTL) design into the gate-level circuit design.
摘要翻译：描述了用于估计电子系统设计的时间，面积和功率特性的知识产权（IP）发生器的方法和装置。 IP生成器接收用户提供的文件，该文件具有描述具有多层次结构的IP设计的配置的数据。 IP生成器还接收用户提供的技术参数和数据流信息。 IP生成器根据用户提供的技术参数，数据流信息和配置参数，关联每个IP子组件的估计时序，面积和功率特性。在将寄存器传输级别（RTL）设计转换为门级电路设计之前，IP生成器通过图形用户界面向用户报告定时，面积和功率估计。

2. 发明申请

US20070037248A1 Production of modified glycoproteins having multiple antennary structures 失效
标题翻译：具有多个触角结构的修饰的糖蛋白的生产
公开(公告)号：US20070037248A1
公开(公告)日：2007-02-15
申请号：US10546101
申请日：2004-02-20
申请人： Piotr Bobrowicz , Stephen Hamilton , Tillman Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Nett , Robert Davidson
发明人： Piotr Bobrowicz , Stephen Hamilton , Tillman Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Nett , Robert Davidson
IPC分类号： C07K14/47 , C07H21/04 , C12P21/06 , C12N1/18 , C12N15/74
CPC分类号： C12P21/005 , C12N9/1051
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
摘要翻译：本发明涉及具有修饰的寡糖的真核宿主细胞，特别是较低的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖和糖核苷酸转运蛋白进一步修饰，以成为用于产生哺乳动物的宿主菌株，人类治疗糖蛋白。该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。制备或选择具有修饰的脂质连接寡糖的宿主细胞。在工程化宿主细胞中制备的N-聚糖表现出GnTIII，GnTIV，GnTV，GnT VI或GnTIX活性，其产生二等分和/或多元N-聚糖结构，并且可以通过异源表达一种或多种酶，例如糖基转移酶，糖，糖核苷酸转运蛋白，以产生人样糖蛋白。为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

3. 外观设计

USD830941S1 Wheel 有权
公开(公告)号：USD830941S1
公开(公告)日：2018-10-16
申请号：US29619667
申请日：2017-09-29
申请人： Stephen Hamilton
设计人： Stephen Hamilton

4. 发明申请

US20120064568A1 ENDOMANNOSIDASES IN THE MODIFICATION OF GLYCOPROTEINS IN EUKARYOTES 审中-公开
标题翻译：乙酰胆碱酯酶在内毒素修饰中的作用
公开(公告)号：US20120064568A1
公开(公告)日：2012-03-15
申请号：US13070946
申请日：2011-03-24
申请人： Stephen Hamilton
发明人： Stephen Hamilton
IPC分类号： C12P21/00 , C12N15/63 , C12N9/96 , C12N5/10 , C12P1/02 , C12N1/19 , C12N1/13 , C12N1/21 , C12P1/00 , C12P1/04 , C12N15/56 , C12N1/15
CPC分类号： C12P21/005 , C12N9/1051 , C12N9/2488 , C12Y302/0113
摘要： The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.
摘要翻译：本发明一般涉及改变在真菌或其他低等真核生物中表达的重组蛋白的糖基化结构的方法，以更加类似于来自较高哺乳动物，特别是人的蛋白质的糖基化。本发明还涉及新的酶和编码它们的核酸，以及工程化表达酶的宿主，在宿主中产生修饰的糖蛋白的方法和如此制备的修饰的糖蛋白。

5. 发明申请

US20100062516A1 Expression of Class 2 Mannosidase and Class III Mannosidase in Lower Eukaryotic Cells 有权
标题翻译： 2种类型的Mannosidase和III类Mannosidase在低等真核细胞中的表达
公开(公告)号：US20100062516A1
公开(公告)日：2010-03-11
申请号：US12536911
申请日：2009-08-06
申请人： Stephen Hamilton
发明人： Stephen Hamilton
IPC分类号： C12N1/19 , C12N1/15
CPC分类号： C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
摘要： A method for producing human-like glycoproteins by expressing a Class 2 α-mannosidase having a substrate specificity for Manα1,3 and Manα1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
摘要翻译：公开了通过在低等真核生物中表达具有Manα1,3和Manα1,6糖苷键的底物特异性的2级α-甘露糖苷酶来生产人样糖蛋白的方法。在寡糖上的这些键合的水解产生用于在分泌途径中进一步N-聚糖加工的底物。

6. 发明申请

US20090130709A1 Endomannosidases in the modification of glycoproteins in eukaryotes 审中-公开
标题翻译：在真核生物中糖蛋白修饰中的胞苷酶
公开(公告)号：US20090130709A1
公开(公告)日：2009-05-21
申请号：US12009105
申请日：2008-01-16
申请人： Stephen Hamilton
发明人： Stephen Hamilton
IPC分类号： C12P21/04 , C12N5/06 , C12N5/04 , C12N1/19 , C12N1/12 , C12N1/21
CPC分类号： C12P21/005 , C12N9/1051 , C12N9/2488 , C12Y302/0113
摘要： The present invention generally relates to methods of modifying the glycosylation structures of recombinant proteins expressed in fungi or other lower eukaryotes, to more closely resemble the glycosylation of proteins from higher mammals, in particular humans. The present invention also relates to novel enzymes and, nucleic acids encoding them and, hosts engineered to express the enzymes, methods for producing modified glycoproteins in hosts and modified glycoproteins so produced.
摘要翻译：本发明一般涉及改变在真菌或其他低等真核生物中表达的重组蛋白的糖基化结构的方法，以更加类似于来自较高哺乳动物，特别是人的蛋白质的糖基化。本发明还涉及新的酶和编码它们的核酸，以及工程化表达酶的宿主，在宿主中产生修饰的糖蛋白的方法和如此制备的修饰的糖蛋白。

7. 发明申请

US20060286637A1 Production of sialylated N-glycans in lower eukaryotes 有权
公开(公告)号：US20060286637A1
公开(公告)日：2006-12-21
申请号：US11429672
申请日：2006-05-05
申请人： Stephen Hamilton
发明人： Stephen Hamilton
IPC分类号： C12P21/06 , C07H21/04 , C12N9/10 , C07K14/47
CPC分类号： C12P21/005 , C07K2319/036 , C07K2319/05 , C07K2319/055 , C12N9/1081 , C12N9/2402 , C12Y302/01018
摘要： The present invention relates to eukaryotic host cells which have been modified to produce sialylated glycoproteins by the heterologous expression of a set of glycosyltransferases, including sialyltransferase and/or trans-sialidase, to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. Novel eukaryotic host cells expressing a CMP-sialic acid biosynthetic pathway for the production of sialylated glycoproteins are also provided. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities (such as those involved in sialylation) to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation.

8. 发明申请

US20060040353A1 Production of galactosylated glycoproteins in lower eukaryotes 有权
标题翻译：低等真核生物中半乳糖基化糖蛋白的产生
公开(公告)号：US20060040353A1
公开(公告)日：2006-02-23
申请号：US11108088
申请日：2005-04-15
申请人： Robert Davidson , Tillman Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Nett , Piotr Bobrowicz , Stephen Hamilton
发明人： Robert Davidson , Tillman Gerngross , Stefan Wildt , Byung-Kwon Choi , Juergen Nett , Piotr Bobrowicz , Stephen Hamilton
IPC分类号： C07H21/04 , C12P21/06 , C12P19/28 , C12N9/10 , C12N1/18 , C12N15/74
CPC分类号： C12P21/005 , C12N9/1051 , C12N15/1082 , C12N15/79 , Y10S435/911
摘要： The present invention provides a novel lower eukaryotic host cell producing human-like glycoproteins characterized as having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues. The present invention also provides a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein.
摘要翻译：本发明提供了一种产生人样糖蛋白的新型低级真核宿主细胞，其特征在于具有末端β-半乳糖残基并且基本上缺乏岩藻糖和唾液酸残基。本发明还提供了一种催化将半乳糖残基从UDP-半乳糖转移到可用作治疗性糖蛋白的重组低等真核宿主细胞中的受体底物上的方法。

9. 外观设计

USD830937S1 Wheel 有权
公开(公告)号：USD830937S1
公开(公告)日：2018-10-16
申请号：US29619664
申请日：2017-09-29
申请人： Stephen Hamilton
设计人： Stephen Hamilton

10. 发明申请

US20130295608A1 YEAST STRAIN FOR THE PRODUCTION OF PROTEINS WITH MODIFIED O-GLYCOSYLATION 有权
标题翻译： YEAST菌株生产具有改性O-糖蛋白的蛋白质
公开(公告)号：US20130295608A1
公开(公告)日：2013-11-07
申请号：US13978714
申请日：2012-02-20
申请人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
发明人： Piotr Bobrowicz , William J. Cook , Stephen Hamilton , Juergen Nett , Terrance A. Stadheim , Stefan Wildt
IPC分类号： C07K14/39
CPC分类号： C07K14/39 , C12N9/90 , C12P21/005
摘要： Lower eukaryotic host cells have been recombinantly engineered to produce glycoprotein having human-like O-glycosylation. The glycoproteins are useful for the production of glycoprotein compositions with advantages for the production of human therapeutics.
摘要翻译：已经重组设计了较低真核宿主细胞以产生具有人类O-糖基化的糖蛋白。糖蛋白可用于生产具有用于生产人类治疗剂的优点的糖蛋白组合物。

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