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1. 发明申请

US20150111253A1 ENZYMATIC OMEGA-OXIDATION AND OMEGA-AMINATION OF FATTY ACIDS 有权
标题翻译：脂肪酸的氧化欧米茄氧化和欧米加氨基酸
公开(公告)号：US20150111253A1
公开(公告)日：2015-04-23
申请号：US14384301
申请日：2013-03-12
申请人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
发明人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
IPC分类号： C12P7/64 , C07K14/21
CPC分类号： C12P7/6436 , C07K14/21 , C07K14/79 , C12N9/0006 , C12N9/0016 , C12N9/0077 , C12N9/0095 , C12N9/1096 , C12P7/42 , C12P7/62 , C12P7/6409 , C12P13/005 , C12Y114/14001
摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.
摘要翻译：本发明涉及一种氧化式（I）的H3C-（CH2）n-COOR的脂肪酸或其酯的方法，其中R选自H，甲基，乙基，丙基和丁基，其中 n为0至30，优选6至24，包括通过在分子氧和NAD存在下使脂肪酸或其酯与CYP153家族的细胞色素P450单加氧酶接触来氧化脂肪酸或其酯的步骤 P）H和表达CYP153家族的重组细胞色素P450单加氧酶，重组醇脱氢酶，重组转氨酶以及任选的一种或多于一种重组酶的全细胞催化剂，其包含丙氨酸脱氢酶，铁氧还蛋白和铁氧还蛋白还原酶，以及所述全细胞催化剂用于氧化脂肪酸或其酯的用途。

2. 发明授权

US09611489B2 Enzymatic omega-oxidation and omega-amination of fatty acids 有权
公开(公告)号：US09611489B2
公开(公告)日：2017-04-04
申请号：US14384301
申请日：2013-03-12
申请人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
发明人： Steffen Schaffer , Michaela Hauberg , Mirja Wessel , Hans-Georg Hennemann , Jan Christoph Pfeffer , Thomas Haas , Harald Haeger
IPC分类号： C12P7/64 , C12N9/04 , C12N9/02 , C12N9/10 , C12P7/42 , C12P7/62 , C07K14/79 , C12N9/06 , C12P13/00 , C07K14/21
CPC分类号： C12P7/6436 , C07K14/21 , C07K14/79 , C12N9/0006 , C12N9/0016 , C12N9/0077 , C12N9/0095 , C12N9/1096 , C12P7/42 , C12P7/62 , C12P7/6409 , C12P13/005 , C12Y114/14001
摘要： The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C —(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

3. 发明申请

US20150111254A1 METHOD FOR AEROBICALLY PRODUCING ALANINE OR A COMPOUND PRODUCED USING ALANINE 有权
标题翻译：用于生产亚麻酸的方法或使用阿兰生产的化合物
公开(公告)号：US20150111254A1
公开(公告)日：2015-04-23
申请号：US14390133
申请日：2013-03-25
申请人： Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Markus Pötter , Jan Christoph Pfeffer , Thomas Haas , Jasmin Corthals , Eva-Maria Eckl , Silvana Roeder , Wolfgang Kroutil , Arne Skerra
发明人： Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Markus Pötter , Jan Christoph Pfeffer , Thomas Haas , Jasmin Corthals , Eva-Maria Eckl , Silvana Roeder , Wolfgang Kroutil , Arne Skerra
IPC分类号： C12P13/06
CPC分类号： C12P13/06 , C12P13/005
摘要： The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell.
摘要翻译：本发明解决的问题在第一方面通过一种制备丙氨酸或使用丙氨酸生产的化合物的方法来解决，该方法具有以下步骤：（a）提供表达重组丙氨酸脱氢酶的细胞或其变体，（b）培养在有氧条件下在无水氮源存在下在水相中，和（c）使细胞与有机相接触，所述细胞是原核或低等真核细胞。

4. 发明申请

US20140256904A1 BIOTECHNOLOGICAL SYNTHESIS PROCESS OF OMEGA-FUNCTIONALIZED CARBON ACIDS AND CARBON ACID ESTERS FROM SIMPLE CARBON SOURCES 审中-公开
标题翻译：来自简单碳源的欧米茄官能化碳酸和碳酸酯的生物合成合成方法
公开(公告)号：US20140256904A1
公开(公告)日：2014-09-11
申请号：US14238591
申请日：2012-08-15
申请人： Steffen Schaffer , Nicole Decker , Jasmin Gielen , Harald Haeger , Thomas Haas , Markus Poetter , Hans-Georg Hennemann , Mirja Wessel , Michael Volland
发明人： Steffen Schaffer , Nicole Decker , Jasmin Gielen , Harald Haeger , Thomas Haas , Markus Poetter , Hans-Georg Hennemann , Mirja Wessel , Michael Volland
IPC分类号： C12P13/00 , C12P13/02 , C08G69/08 , C12P7/64
CPC分类号： C12P13/02 , C08G69/08 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0014 , C12N9/0077 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/40 , C12P7/42 , C12P7/6409 , C12P7/6436 , C12P13/005 , Y02P20/52
摘要： The subject of the invention is a biotechnological process for the production of ω-functionalized carboxylic acids and of ω-functionalized carboxylate esters from simple carbon sources.
摘要翻译：本发明的主题是用于从简单碳源生产ω官能化羧酸和ω-官能化羧酸酯的生物技术方法。

5. 发明授权

US09719117B2 Production of omega-amino fatty acids 有权
公开(公告)号：US09719117B2
公开(公告)日：2017-08-01
申请号：US14132473
申请日：2013-12-18
申请人： Steffen Schaffer , Jasmin Gielen , Mirja Wessel , Hans-Georg Hennemann , Harald Haeger , Thomas Haas , Wilfried Bluemke
发明人： Steffen Schaffer , Jasmin Gielen , Mirja Wessel , Hans-Georg Hennemann , Harald Haeger , Thomas Haas , Wilfried Bluemke
IPC分类号： C12P13/00 , C12N9/12 , C12N9/02 , C12N9/06 , C12N9/10
CPC分类号： C12P13/001 , C12N9/0008 , C12N9/0016 , C12N9/0069 , C12N9/1096 , C12N9/1288 , C12P13/005 , Y02P20/582
摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

6. 发明授权

US09249435B2 Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium 有权
标题翻译：改进疏水性有机溶液与水性培养基分离的方法
公开(公告)号：US09249435B2
公开(公告)日：2016-02-02
申请号：US13721481
申请日：2012-12-20
申请人： Jasmin Gielen , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
发明人： Jasmin Gielen , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
IPC分类号： C12P13/04 , C12P7/64 , C12N9/02
CPC分类号： C12P7/6436 , C12N9/001 , C12P13/04 , C12Y103/99003
摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.
摘要翻译：提供了一种从水性培养基中改善疏水性有机溶液分离的方法。该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。与野生型活性细胞相比，代谢活性细胞的活性降低，活性降低至少是催化脂肪酸一次反应的一种酶。本发明进一步提供了一种代谢活性细胞，其与野生型相比具有降低的活性，其酶催化脂肪酸的一种反应的反应，包括选自FadA，FadB，FadD的酶，FadL和FadE及其变体。

7. 发明申请

US20130164797A1 PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM 有权
标题翻译：从水质培养基中改善疏水有机溶液分离的方法
公开(公告)号：US20130164797A1
公开(公告)日：2013-06-27
申请号：US13721481
申请日：2012-12-20
申请人： Jasmin GIELEN , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
发明人： Jasmin GIELEN , Thomas Haas , Hans-Georg Hennemann , Steffen Schaffer , Mirja Wessel , Katharina Berse , Marion Schiebelhut , Sabine Hafkemeyer , Markus Poetter , Frank Erhardt
IPC分类号： C12P7/64
CPC分类号： C12P7/6436 , C12N9/001 , C12P13/04 , C12Y103/99003
摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.
摘要翻译：提供了一种从水性培养基中改进疏水性有机溶液分离的方法。该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。代谢活性细胞的活性降低与野生型活性细胞相比，活性降低至少是一种催化脂肪酸β-氧化反应的一种酶。本发明还提供了一种代谢活性细胞，其与野生型相比具有降低的活性，所述活性细胞能催化脂肪酸的β-氧化反应之一，包括选自FadA，FadB，FadD， FadL和FadE及其变体。

8. 发明申请

US20150218600A1 BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译： 3-羟基丁酸的生物技术制备
公开(公告)号：US20150218600A1
公开(公告)日：2015-08-06
申请号：US14363178
申请日：2012-11-14
申请人： Thomas Haas , Steffen Schaffer , Markus Poetter , Mirja Wessel , Jan Christoph Pfeffer , Christian Gehring , Nicole Kirchner , Eva Maria Wittmann
发明人： Thomas Haas , Steffen Schaffer , Markus Poetter , Mirja Wessel , Jan Christoph Pfeffer , Christian Gehring , Nicole Kirchner , Eva Maria Wittmann
IPC分类号： C12P7/42 , C12N9/88 , C12N9/12 , C12N9/02 , C12N9/00 , C12N9/10
CPC分类号： C12P7/42 , C12N9/0004 , C12N9/001 , C12N9/1217 , C12N9/13 , C12N9/88 , C12N9/93 , C12N15/52 , C12Y103/99002 , C12Y207/02007 , C12Y402/01017 , C12Y602/01002
摘要： The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid.
摘要翻译：本发明涉及包括以下步骤的方法：a）提供异丁酸，b）使异丁酸与异丁酸酯激酶和磷酸转移异丁酰基酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸酯辅酶A转移酶c的组合接触）使来自步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）将来自步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）中的产物以形成3-羟基异丁酸酸，其中至少一种酶以细胞形式使用，与其野生型相比，其包含3-羟基异丁酸脱氢酶或其变体的降低的活性，具有至少一种酶的细胞异丁酰辅酶A合成酶/连接酶，异丁酸酯辅酶A转移酶，异丁酸酯激酶，磷酸转移异丁酰基酶，异丁酰辅酶A脱氢酶，甲基丙烯酰辅酶A水合酶和 3-羟基异丁酰辅酶A水解酶，与其野生型相比，3-羟基异丁酸脱氢酶或其变体的活性降低，其中细胞优选还具有单加氧酶，更优选alkBGT型的单加氧酶或其变体以及这种细胞用于制备3-羟基异丁酸的用途。

9. 发明申请

US20130052700A1 BIOCATALYTIC OXIDATION PROCESS WITH ALKL GENE PRODUCT 有权
标题翻译：含ALKL基因产物的生物氧化氧化过程
公开(公告)号：US20130052700A1
公开(公告)日：2013-02-28
申请号：US13642412
申请日：2011-03-15
申请人： Markus Poetter , Andreas Schmid , Bruno Buehler , Hans-Georg Hennemann , Mattijs Kamiel Julsing , Steffen Schaffer , Thomas Haas , Manfred Schrewe , Sjef Cornelissen , Martin Roos , Harald Haeger
发明人： Markus Poetter , Andreas Schmid , Bruno Buehler , Hans-Georg Hennemann , Mattijs Kamiel Julsing , Steffen Schaffer , Thomas Haas , Manfred Schrewe , Sjef Cornelissen , Martin Roos , Harald Haeger
IPC分类号： C12P7/62 , C12P7/26 , C12P7/24 , C12P7/04 , C12P13/00 , C12P7/64 , C12N1/21 , C12N1/13 , C12P7/40 , C12P7/02
CPC分类号： C07K14/21 , C12P7/02 , C12P7/24 , C12P7/42 , C12P7/62 , C12P7/6436 , C12Y114/15003
摘要： The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.
摘要翻译：本发明提供了借助于alkL基因产物和在该方法中使用的微生物来氧化有机化合物的生物催化方法。

10. 发明授权

US08999684B2 Candida tropicalis cells and use thereof 有权
标题翻译：热带假丝酵母细胞及其用途
公开(公告)号：US08999684B2
公开(公告)日：2015-04-07
申请号：US13764996
申请日：2013-02-12
申请人： Markus Poetter , Hans-Georg Hennemann , Steffen Schaffer , Thomas Haas
发明人： Markus Poetter , Hans-Georg Hennemann , Steffen Schaffer , Thomas Haas
IPC分类号： C12P7/40 , C12P13/04 , C12N1/16 , C12N9/02 , C12P7/42 , C12P7/62
CPC分类号： C12P7/62 , C07C51/347 , C07C67/42 , C07C227/06 , C07C227/08 , C12N1/16 , C12N9/004 , C12N9/0042 , C12P7/42 , C12P7/625 , C12P13/04 , C12Y101/01001
摘要： The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.
摘要翻译：本发明涉及基因工程的热带假丝酵母细胞，其用途和生产ω-羟基羧酸和ω-羟基羧酸酯的方法。

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