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1. 发明授权

US06468432B1 Methods of modification of selected cells in a magnetic cell separation column 有权
标题翻译：在磁性细胞分离柱中修饰所选择的细胞的方法
公开(公告)号：US06468432B1
公开(公告)日：2002-10-22
申请号：US09654186
申请日：2000-09-01
申请人： Stefan Miltenyi , Mario Assenmacher , Jürgen Schmitz
发明人： Stefan Miltenyi , Mario Assenmacher , Jürgen Schmitz
IPC分类号： C02F148
CPC分类号： C12N13/00 , B03C1/002 , B03C1/01 , B03C1/034 , C12N2510/00 , Y10S436/806
摘要： A method is provided for modifying cells retained in a high gradient magnetic cell separation column (HGMS). Selected cells within a heterogeneous mixture are labeled with a label comprising a magnetic particle and specific for the selected cells. The labeled, selected cells are applied to the magnetic cell separation column, which retains the selected cells. The selected cells are then modified while retained in the column and then removed to the original suspension or to a purified, homogenous suspension.
摘要翻译：提供了用于修饰保留在高梯度磁性细胞分离柱（HGMS）中的细胞的方法。不均匀混合物中的选定细胞用包含磁性颗粒并且对所选细胞特异的标记进行标记。将标记的所选择的细胞施用于保留所选择的细胞的磁性细胞分离柱。然后将所选择的细胞修饰，同时保留在柱中，然后除去至原始悬浮液或纯化的均匀悬浮液。

2. 发明授权

US06576428B1 Direct selection of antigen-specific T cells, compositions obtained thereby and methods of use thereof 有权
标题翻译：抗原特异性T细胞的直接选择，由此获得的组合物及其使用方法
公开(公告)号：US06576428B1
公开(公告)日：2003-06-10
申请号：US09309199
申请日：1999-05-10
申请人： Mario Assenmacher , Stefan Miltenyi , Jurgen Schmitz
发明人： Mario Assenmacher , Stefan Miltenyi , Jurgen Schmitz
IPC分类号： G01N3353
CPC分类号： G01N33/56966 , C12N5/0636 , G01N33/5005 , G01N33/505
摘要： The invention provides a method for convenient analysis and cell separation of antigen-specific T cells based on one or more products secreted by these cells in response to antigen stimulation. The T cells are provided with a capture moiety for the product, which can then be used directly as a label in some instances, or the bound product can be further labeled via label moieties that bind specifically to the product and that are labeled with traditional labeling materials such as fluorophores, radioactive isotopes, chromophores or magnetic particles. The labeled cells are then separated using standard cell sorting techniques based on these labels. Such techniques include flow cytometry, magnetic gradient separation, centrifugation, and the like.
摘要翻译：本发明提供了一种基于一种或多种由这些细胞响应于抗原刺激分泌的产物的方法分析和细胞分离抗原特异性T细胞的方法。为T细胞提供产品的捕获部分，然后可以在某些情况下直接用作标记，或者可以通过与产物特异性结合的标记部分进一步标记结合产物，并用传统标记材料如荧光团，放射性同位素，发色团或磁性颗粒。然后使用基于这些标记的标准细胞分选技术分离标记的细胞。这些技术包括流式细胞术，磁梯度分离，离心等。

3. 发明授权

US06190870B1 Efficient enrichment and detection of disseminated tumor cells 失效
公开(公告)号：US06190870B1
公开(公告)日：2001-02-20
申请号：US08667464
申请日：1996-06-21
申请人： Jürgen Schmitz , Stefan Miltenyi , Fawn Qian , Aaron Kantor
发明人： Jürgen Schmitz , Stefan Miltenyi , Fawn Qian , Aaron Kantor
IPC分类号： G01N33553
CPC分类号： G01N33/54326 , G01N33/574 , Y10S435/962 , Y10S436/813 , Y10S436/824 , Y10S436/825
摘要： Tumor cells, particularly carcinoma cells, are separated from peripheral blood by magnetic sorting. The tumor cells are magnetically labeled with antibodies directed to tissue specific antigens, preferably cytoplasmic proteins. Labeling for cytoplasmic antigens is accomplished first permeabilizing, then fixing the cells. The cells are separated on a magnetic matrix. The number of tumor cells in the enriched fraction is used to calculate the number of tumor cells present in a patient hematopoietic sample.

4. 发明申请

US20110003380A1 Sample Processing System and Methods 有权
标题翻译：样品处理系统和方法
公开(公告)号：US20110003380A1
公开(公告)日：2011-01-06
申请号：US12745688
申请日：2008-12-08
申请人： Stefan Miltenyi , Winfried Schimmelpfennig , Holger Lantow , Niklas Elmar , Martin Biehl , Eiad Kabaha , Jürgen Schulz
发明人： Stefan Miltenyi , Winfried Schimmelpfennig , Holger Lantow , Niklas Elmar , Martin Biehl , Eiad Kabaha , Jürgen Schulz
IPC分类号： C12N5/00 , C12M1/00 , C12M1/34
CPC分类号： G01N33/58 , A61M1/0209 , A61M1/3618 , A61M1/3633 , A61M1/3679 , A61M1/3692 , A61M1/3693 , A61M1/3696 , A61M2202/0071 , A61M2202/0413 , A61M2202/0427 , A61M2202/10 , B01D15/3809 , B01D15/3885 , B01D21/26 , B03C1/002 , B03C1/027 , B03C1/032 , B03C1/0332 , B03C1/0335 , B03C1/034 , B03C1/288 , B03C2201/18 , B03C2201/26 , B04B1/12 , B04B5/00 , B04B5/04 , B04B5/0442 , B04B5/10 , B04B7/08 , B04B13/00 , B04B2005/0464 , B04B2013/006 , C12M23/28 , C12M33/10 , C12M41/48 , C12M47/04 , C12N5/0636 , C12N5/0646 , C12N5/0647 , C12N5/0669 , G01N35/0098 , G01N2333/70596
摘要： The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.
摘要翻译：本发明涉及一种系统，包括：a）样品处理单元，包括输入端口和耦合到具有至少一个样品室的旋转容器的输出端口，所述样品处理单元配置为样品提供第一处理步骤或使容器旋转以对沉积在室中的样品施加离心力，并分离沉积样品的至少第一组分和第二组分; 以及b）样品分离单元，其耦合到所述样品处理单元的输出端口，所述细胞分离单元包括分离柱保持器（42），泵（64）和多个阀（1-11），其被配置为至少部分地控制流体流过流体回路和位于所述保持器中的分离柱（40），所述分离柱构造成分离流过所述塔的样品的标记和未标记组分。

5. 发明授权

US6020210A Methods and materials for high gradient magnetic separation of biological materials 失效
标题翻译：生物材料高梯度磁选分离方法与材料
公开(公告)号：US6020210A
公开(公告)日：2000-02-01
申请号：US377774
申请日：1995-01-23
申请人： Stefan Miltenyi
发明人： Stefan Miltenyi
IPC分类号： B03C1/00 , B03C1/01 , B03C1/033 , B03C1/034 , G01N33/543 , G01N33/553 , G01N33/48 , G01N33/567 , A61L3/00
CPC分类号： G01N33/5434 , B03C1/002 , B03C1/01 , B03C1/0332 , B03C1/034 , G01N2400/22 , G01N2446/20 , G01N2446/30 , G01N2446/90 , Y10S436/807 , Y10S436/824
摘要： Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. The use of plastic coated matrices especially small spheres or balls which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS. The selection of small spheres in combination with the coating provides for uniform matrices of high stability.
摘要翻译：公开了用于进行高梯度磁选（HGMS）的现有程序和材料的改进。公开了使用形成优异的磁梯度增强载体的塑料涂层基质，特别是小球或球，以及改进的用于进行HGMS的方法和装置。与涂层组合的小球的选择提供了高稳定性的均匀基质。

6. 发明申请

US20060141540A1 Direct selection of cells by secretion product 审中-公开
标题翻译：通过分泌产物直接选择细胞
公开(公告)号：US20060141540A1
公开(公告)日：2006-06-29
申请号：US11359328
申请日：2006-02-21
申请人： Stefan Miltenyi , Andreas Radbruch , Rudi Manz
发明人： Stefan Miltenyi , Andreas Radbruch , Rudi Manz
IPC分类号： G01N33/567 , C12N5/06
CPC分类号： G01N33/5005 , G01N33/53 , G01N33/56966 , G01N33/56972 , G01N33/58
摘要： Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled cells can then be further coupled to suitable labels, if desired, and separated according to the presence, absence, or amount of product.
摘要翻译：细胞可以通过将其表面上的细胞与产物的特异性结合配偶体偶联而以有效的方式分泌和释放的产物进行标记，并允许产物在分泌和释放时被特异性结合配偶体捕获。然后，如果需要，将产物标记的细胞进一步偶合到合适的标记上，并根据产品的存在，不存在或数量分离。

7. 发明授权

US5779892A Magnetic separator with magnetic compensated release mechanism for separating biological material 失效
标题翻译：具有用于分离生物材料的磁补偿释放机构的磁选机
公开(公告)号：US5779892A
公开(公告)日：1998-07-14
申请号：US749573
申请日：1996-11-15
申请人： Stefan Miltenyi , Gerard Buchholz , Robert Herz
发明人： Stefan Miltenyi , Gerard Buchholz , Robert Herz
IPC分类号： B03C1/033 , B01P35/06
CPC分类号： B03C1/0332 , B03C1/033
摘要： A magnetic separator device is provided for separating biological materials from carrier fluids using the technique of high gradient magnetic separation. A release compensator reduces the amount of force that an operator needs to apply in order to remove a separation column from the magnetic field of the separator device. The release compensator may be a mechanical compensator, but is preferably a magnetic compensator. The magnetic separator device also preferably includes a mount for mounting the device to a wall. The mount is preferably a pair of additional magnets.
摘要翻译：提供了一种使用高梯度磁选法分离生物材料与载体流体的磁选机。释放补偿器减少了操作者为了从分离器装置的磁场中移除分离柱而需要施加的力的量。释放补偿器可以是机械补偿器，但优选地是磁补偿器。磁选机装置还优选地包括用于将装置安装到墙壁上的支架。该安装件优选地是一对额外的磁体。

8. 发明授权

US5711871A Magnetic separation apparatus 失效
标题翻译：磁选机
公开(公告)号：US5711871A
公开(公告)日：1998-01-27
申请号：US487277
申请日：1995-06-07
申请人： Stefan Miltenyi
发明人： Stefan Miltenyi
IPC分类号： A61M1/36 , B01D35/06 , B03C1/00 , B03C1/025 , G01N1/34 , G01N33/543
CPC分类号： B03C1/002 , A61M1/3618 , A61M1/362 , B01D35/06 , B03C1/025 , G01N33/54326 , A61M1/36 , G01N1/34 , Y10S436/806
摘要： Improved magnetic separation devices for magnetic separation procedures are provided. The improved separation devices contain matrices which provide uniform pores or channels that reduce the entrapment of air or non-target substances, and decrease the loss of target substances due to mechanical disruption. Target cells, from various systems and organs, or other target biological substances are labeled in conjunction with a suitable specific binding member, and isolated using the devices and methods of the present invention. In its simplest form, the cell separation system of the present invention has two main components: a magnetic separator and a cell separation reagent. A more complex separation device includes fluid passages, collection and storage containers and the separation column. The fluid circuitry can be constructed with integrated valves, or the valves may be applied externally to the fluid pathways.
摘要翻译：提供了用于磁分离程序的改进的磁分离装置。改进的分离装置包含提供均匀的孔或通道的基质，其减少空气或非目标物质的捕获，并减少由于机械破坏导致的目标物质的损失。来自各种系统和器官的靶细胞或其它目标生物物质与合适的特异性结合成员一起标记，并使用本发明的装置和方法进行分离。在其最简单的形式中，本发明的细胞分离系统具有两个主要成分：磁选机和细胞分离试剂。更复杂的分离装置包括流体通道，收集和储存容器以及分离塔。流体回路可以用集成阀构成，或者阀可以在流体路径的外部施加。

9. 发明授权

US08747290B2 Centrifuge for separating a sample into at least two components 失效
标题翻译：用于将样品分离成至少两个组分的离心机
公开(公告)号：US08747290B2
公开(公告)日：2014-06-10
申请号：US12745689
申请日：2008-12-08
申请人： Stefan Miltenyi , Winfried Schimmelpfennig , Holger Lantow , Niklas Elmar Neuschäfer , Martin Biehl , Eiad Kabaha , Jürgen Schulz
发明人： Stefan Miltenyi , Winfried Schimmelpfennig , Holger Lantow , Niklas Elmar Neuschäfer , Martin Biehl , Eiad Kabaha , Jürgen Schulz
IPC分类号： B04B1/12 , B04B7/08 , B04B5/04 , B04B13/00
CPC分类号： G01N33/58 , A61M1/0209 , A61M1/3618 , A61M1/3633 , A61M1/3679 , A61M1/3692 , A61M1/3693 , A61M1/3696 , A61M2202/0071 , A61M2202/0413 , A61M2202/0427 , A61M2202/10 , B01D15/3809 , B01D15/3885 , B01D21/26 , B03C1/002 , B03C1/027 , B03C1/032 , B03C1/0332 , B03C1/0335 , B03C1/034 , B03C1/288 , B03C2201/18 , B03C2201/26 , B04B1/12 , B04B5/00 , B04B5/04 , B04B5/0442 , B04B5/10 , B04B7/08 , B04B13/00 , B04B2005/0464 , B04B2013/006 , C12M23/28 , C12M33/10 , C12M41/48 , C12M47/04 , C12N5/0636 , C12N5/0646 , C12N5/0647 , C12N5/0669 , G01N35/0098 , G01N2333/70596
摘要： The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber.
摘要翻译：本发明涉及一种用于将样品分离成至少两个组分的离心机，包括用于接收要离心的样品的室。根据本发明，离心机还包括用于控制样品分离进度位于腔室的装置。

10. 发明授权

US08663985B2 Anchoring/capturing system for selecting or analyzing a CHO cell according to a product secreted by the CHO cell 有权
标题翻译：用于根据CHO细胞分泌的产物选择或分析CHO细胞的锚定/捕获系统
公开(公告)号：US08663985B2
公开(公告)日：2014-03-04
申请号：US13098498
申请日：2011-05-02
申请人： Stefan Miltenyi , Susanne Hoher-Peters , Monika Winkels
发明人： Stefan Miltenyi , Susanne Hoher-Peters , Monika Winkels
IPC分类号： C12N5/07 , G01N33/53 , G01N33/574
CPC分类号： G01N33/54306
摘要： An anchoring/capturing system for selecting or analyzing a CHO cell according to a product secreted by the CHO cell is described. The anchoring/capturing system comprises a first antibody or a first antigen-binding fragment for anchoring to the extracellular surface of the CHO cell, and a second antibody or a second antigen-binding fragment for binding the secreted product. Uses and methods involving the anchoring/capturing system are provided.
摘要翻译：描述了根据CHO细胞分泌的产物选择或分析CHO细胞的锚定/捕获系统。锚定/捕获系统包括用于锚定到CHO细胞的细胞外表面的第一抗体或第一抗原结合片段，以及用于结合分泌产物的第二抗体或第二抗原结合片段。提供了涉及锚定/捕获系统的使用和方法。

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