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1. 发明申请

US20060292553A1 Method for study of the genetic and functional variability of HIV and kit for using it 审中-公开
标题翻译：研究HIV遗传和功能变异性的方法及其使用方法
公开(公告)号：US20060292553A1
公开(公告)日：2006-12-28
申请号：US11073972
申请日：2005-03-07
申请人： Sophie Lebel-Binay , Elisabeth Dam , Luc Boblet , Dominique Costantini
发明人： Sophie Lebel-Binay , Elisabeth Dam , Luc Boblet , Dominique Costantini
IPC分类号： C12Q1/70 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/703
摘要： A method of analyzing a sample possibly containing an HIV virus, including a) extracting viral RNA in a biological sample that possibly contains an HIV virus; b) reverse transcription of the RNA obtained of (a) and amplification with a first pair of primers to obtain an amplified product of reverse transcription including all or part of at least two successive genes of a genome of an HIV virus; and one or both of c) and d): c) sequencing the amplified product of (b) to establish a genotype of HIV virus present in the sample and identify mutations that may be present in the amplified product; d1) amplifying the product of (b) with a second pair of primers complementary to the first pair of (b) and capable of generating an amplification product that can be inserted by homologous recombination into a retroviral vector that is defective in a region corresponding to the amplified product; d2) homologously recombining the product of (d1) with the defective vector; d3) functionally analyzing the viral proteins coded by all or part of the at least two successive genes of the product of (d1); and d4) measuring replicating capacity of recombinant viruses of (d2) in the presence or in the absence of at least one active substance.
摘要翻译：分析可能含有HIV病毒的样品的方法，包括a）在可能含有HIV病毒的生物样品中提取病毒RNA; b）用（a）获得的RNA的逆转录和用第一对引物扩增以获得包括HIV病毒基因组的全部或部分至少两个连续基因的逆转录的扩增产物; 和c）和d）中的一个或两个：c）对（b）的扩增产物进行测序以建立存在于样品中的HIV病毒的基因型并鉴定可能存在于扩增产物中的突变; d1）用与第一对（b）互补的第二对引物扩增（b）的产物，并且能够产生可以通过同源重组插入的扩增产物到对应于扩增产物; d2）将（d1）的产物与缺陷载体同源重组; d3）功能性分析由（d1）的产物的全部或部分至少两个连续基因编码的病毒蛋白; 和d4）在存在或不存在至少一种活性物质的情况下测量（d2）重组病毒的复制能力。

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