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1. 发明授权

US08697063B2 Polypeptides having phytase activity and polynucleotides encoding same 有权
标题翻译：具有植酸酶活性的多肽和编码其的多核苷酸
公开(公告)号：US08697063B2
公开(公告)日：2014-04-15
申请号：US13327831
申请日：2011-12-16
申请人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
发明人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
IPC分类号： C12N9/16 , C12P7/06 , C07K14/195 , A61K38/16
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有植酸酶活性的多肽。这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。这些植酸酶具有改善的比活性。还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能。本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。

2. 发明申请

US20110154519A1 HAFNIA PHYTASE 有权
公开(公告)号：US20110154519A1
公开(公告)日：2011-06-23
申请号：US13032679
申请日：2011-02-23
申请人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
发明人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
IPC分类号： C12N9/14 , C12N15/11 , C12N15/55 , C12N1/00 , C12P21/02 , A01H5/00 , A01K67/00 , A23K1/16 , A23K1/165 , A23L1/302 , A23L1/305 , A23L1/304 , C12G1/00 , C12C11/00 , A23K1/06
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K50/00 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C12Y301/01004 , C12Y301/01005 , C12Y301/03008 , C12Y301/03026 , C12Y301/04003 , C12Y301/04004 , C12Y302/01001 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01022 , C12Y302/01032 , C12Y302/01089 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.
摘要翻译：本发明涉及具有植酸酶活性的分离的多肽和编码多肽的分离的多核苷酸。多肽与源自Hafnia alvei的植酸酶相关，其氨基酸序列在附加的序列表中显示为SEQ ID NO：10。本发明还涉及核酸构建体，载体和宿主细胞，其包含多核苷酸为以及用于产生和使用多肽的方法，特别是在动物饲料中。

3. 发明申请

US20080222740A1 Polypeptides Having Phytase Activity And Polynucleotides Encoding Same 有权
标题翻译：具有植酸酶活性的多肽和编码相同的多核苷酸
公开(公告)号：US20080222740A1
公开(公告)日：2008-09-11
申请号：US12022221
申请日：2008-01-30
申请人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
发明人： Carsten Sjoeholm , Soeren Flensted Lassen , Lars Kobberoee Skov , Leonardo De Maria
IPC分类号： A01K67/00 , C12N9/14 , C12N9/50 , C12N9/28 , C12N9/26 , A23J1/00 , A23K1/00 , C12P7/06 , C07H21/04 , C12N15/00 , C12N5/00 , C12P21/04 , A01H5/00
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有植酸酶活性的多肽。这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。这些植酸酶具有改善的比活性。还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能。本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。

4. 发明授权

US08663963B2 Hafnia phytase 有权
标题翻译： Hafnia植酸酶
公开(公告)号：US08663963B2
公开(公告)日：2014-03-04
申请号：US13032679
申请日：2011-02-23
申请人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
发明人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
IPC分类号： C12N9/16 , A23J1/00 , A61K38/46
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K50/00 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C12Y301/01004 , C12Y301/01005 , C12Y301/03008 , C12Y301/03026 , C12Y301/04003 , C12Y301/04004 , C12Y302/01001 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01022 , C12Y302/01032 , C12Y302/01089 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.
摘要翻译：本发明涉及具有植酸酶活性的分离的多肽和编码多肽的分离的多核苷酸。多肽与源自Hafnia alvei的植酸酶相关，其氨基酸序列在附加的序列表中显示为SEQ ID NO：10。本发明还涉及核酸构建体，载体和宿主细胞，其包含多核苷酸为以及用于产生和使用多肽的方法，特别是在动物饲料中。

5. 发明授权

US07923232B2 Hafnia phytase 有权
标题翻译： Hafnia植酸酶
公开(公告)号：US07923232B2
公开(公告)日：2011-04-12
申请号：US12055694
申请日：2008-03-26
申请人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
发明人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
IPC分类号： C12N9/16 , A23J1/00 , A61K38/46
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K50/00 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C12Y301/01004 , C12Y301/01005 , C12Y301/03008 , C12Y301/03026 , C12Y301/04003 , C12Y301/04004 , C12Y302/01001 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01022 , C12Y302/01032 , C12Y302/01089 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.
摘要翻译：本发明涉及具有植酸酶活性的分离的多肽和编码多肽的分离的多核苷酸。多肽与源自Hafnia alvei的植酸酶相关，其氨基酸序列在附加的序列表中显示为SEQ ID NO：10。本发明还涉及核酸构建体，载体和宿主细胞，其包含多核苷酸为以及用于产生和使用多肽的方法，特别是在动物饲料中。

6. 发明申请

US20080263688A1 Hafnia Phytase 有权
标题翻译： Hafnia植酸酶
公开(公告)号：US20080263688A1
公开(公告)日：2008-10-23
申请号：US12055694
申请日：2008-03-26
申请人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
发明人： Soeren Flensted Lassen , Carsten Sjoeholm , Lars Kobberoee Skov
IPC分类号： A01K67/027 , C12N9/16 , C12N15/11 , C12N15/00 , C12P7/10 , A23K1/16 , C12N1/20 , C12P21/04 , A01H5/00
CPC分类号： C12N9/16 , A23K20/147 , A23K20/174 , A23K20/189 , A23K50/00 , A23K50/30 , A23K50/75 , A23K50/80 , A23L33/18 , C12Y301/01004 , C12Y301/01005 , C12Y301/03008 , C12Y301/03026 , C12Y301/04003 , C12Y301/04004 , C12Y302/01001 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01022 , C12Y302/01032 , C12Y302/01089 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to isolated polypeptides having phytase activity and isolated polynucleotides encoding the polypeptides. The polypeptides are related to a phytase derived from Hafnia alvei, the amino acid sequence of which is shown in the appended sequence listing as SEQ ID NO: 10. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides, in particular within animal feed.
摘要翻译：本发明涉及具有植酸酶活性的分离的多肽和编码多肽的分离的多核苷酸。多肽与源自Hafnia alvei的植酸酶相关，其氨基酸序列在附加的序列表中显示为SEQ ID NO：10。本发明还涉及核酸构建体，载体和宿主细胞，其包含多核苷酸为以及用于产生和使用多肽的方法，特别是在动物饲料中。

7. 发明申请

US20100083392A1 HAFNIA PHYTASE VARIANTS 有权
公开(公告)号：US20100083392A1
公开(公告)日：2010-04-01
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： A01K67/00 , C12N9/16 , C12P1/00 , C12P7/06 , C07H21/00 , C12N15/63 , C12N1/00 , C12P21/00 , A01H5/00 , A23L1/28 , A23K1/18 , A23K1/16 , C12G1/00 , C12C11/00 , C12C7/00
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

8. 发明申请

US20110039301A1 Polypeptides Having Organophosphorous Hydrolase Activity and Polynucleotides Encoding Same 审中-公开
标题翻译：具有有机磷水解酶活性的多肽和编码相同的多核苷酸
公开(公告)号：US20110039301A1
公开(公告)日：2011-02-17
申请号：US12988096
申请日：2009-04-23
申请人： Soeren Flensted Lassen , Steffen Danielsen , Lars Henrik Oestergaard , Lars Kobberoee Skov
发明人： Soeren Flensted Lassen , Steffen Danielsen , Lars Henrik Oestergaard , Lars Kobberoee Skov
IPC分类号： C12N9/14 , C12N15/55 , C12N15/63 , C12N1/00 , C12P21/00 , A62D3/02
CPC分类号： C12N9/16 , A62D3/02 , A62D2101/02 , A62D2101/04 , A62D2101/26 , C12N15/8257 , C12N15/8259
摘要： The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译：本发明涉及具有有机磷水解酶活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

9. 发明申请

US20120225468A1 Hafnia Phytase Variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US20120225468A1
公开(公告)日：2012-09-06
申请号：US13472822
申请日：2012-05-16
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/16
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

10. 发明授权

US08206962B2 Hafnia phytase variants 有权
标题翻译： Hafnia植酸酶变体
公开(公告)号：US08206962B2
公开(公告)日：2012-06-26
申请号：US12568312
申请日：2009-09-28
申请人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
发明人： Soeren Flensted Lassen , Leonardo De Maria , Esben Peter Friis , Tomoko Matsui , Allan Noergaard , Lars Kobberoee Skov , Jesper Vind
IPC分类号： C12N9/14 , C12Q1/34 , C07H21/04
CPC分类号： C12N9/16 , A23K20/189 , A23K50/30 , A23K50/75 , Y02E50/17
摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译：本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度简介和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。

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