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1. 发明申请

US20080050770A1 Method for the production and purification of adenoviral vectors 审中-公开
标题翻译：腺病毒载体的生产和纯化方法
公开(公告)号：US20080050770A1
公开(公告)日：2008-02-28
申请号：US11509486
申请日：2006-08-24
申请人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
发明人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
IPC分类号： C12P1/00
CPC分类号： C07K14/4746 , A61K35/761 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2710/10352 , C12N2750/14143
摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
摘要翻译：本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。在其他实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，存在改善的Ad-p53产生。提高产量也很重要的是使用洗涤剂溶解。本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

2. 发明授权

US07125706B2 Method for the production and purification of adenoviral vectors 有权
标题翻译：腺病毒载体的生产和纯化方法
公开(公告)号：US07125706B2
公开(公告)日：2006-10-24
申请号：US09880609
申请日：2001-06-12
申请人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
发明人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
IPC分类号： C12N7/01 , C12N15/861 , A61K39/235
CPC分类号： C07K14/4746 , A61K35/761 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2710/10352 , C12N2750/14143
摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
摘要翻译：本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。在其它实施方案中，发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的Ad-p53生产细胞有改善。提高产量也很重要的是使用洗涤剂溶解。本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

3. 发明授权

US07732129B1 Method for the production and purification of adenoviral vectors 有权
标题翻译：腺病毒载体的生产和纯化方法
公开(公告)号：US07732129B1
公开(公告)日：2010-06-08
申请号：US09203078
申请日：1998-12-01
申请人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
发明人： Shuyuan Zhang , Capucine Thwin , Zheng Wu , Toohyon Cho , Shawn Gallagher
IPC分类号： C12Q1/70
CPC分类号： C07K14/4746 , A61K35/761 , A61K38/00 , A61K48/00 , C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10351 , C12N2710/10352 , C12N2750/14143
摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.
摘要翻译：本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。在其他实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，存在改善的Ad-p53产生。提高产量也很重要的是使用洗涤剂溶解。本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

4. 发明授权

US08090521B2 Method and kit for engine emission control 有权
标题翻译：用于发动机排放控制的方法和试剂盒
公开(公告)号：US08090521B2
公开(公告)日：2012-01-03
申请号：US12632561
申请日：2009-12-07
申请人： Ryan John Goes , Shawn Gallagher , Eric Dillen , Rajani Boddu
发明人： Ryan John Goes , Shawn Gallagher , Eric Dillen , Rajani Boddu
IPC分类号： B60T7/12
CPC分类号： F02D41/0082 , F02D41/22 , F02D41/222 , F02D41/401 , F02D2200/0414 , Y02T10/44
摘要： Methods and systems are provided for operating an engine including a first and a second bank of cylinders. One example method comprises, adjusting engine injection timing based on a first temperature of a first intake of the first bank and a second temperature of a second intake of the second bank.
摘要翻译：提供了用于操作包括第一和第二气缸组的发动机的方法和系统。一个示例性方法包括：基于第一组的第一摄入的第一温度和第二组的第二摄入的第二温度来调节发动机喷射正时。

5. 发明申请

US20110061629A1 METHOD AND KIT FOR ENGINE EMISSION CONTROL 有权
标题翻译：发动机排放控制的方法和工具包
公开(公告)号：US20110061629A1
公开(公告)日：2011-03-17
申请号：US12632561
申请日：2009-12-07
申请人： Ryan John Goes , Shawn Gallagher , Eric Dillen , Rajani Boddu
发明人： Ryan John Goes , Shawn Gallagher , Eric Dillen , Rajani Boddu
IPC分类号： F02M7/00 , F02B33/00
CPC分类号： F02D41/0082 , F02D41/22 , F02D41/222 , F02D41/401 , F02D2200/0414 , Y02T10/44
摘要： Methods and systems are provided for operating an engine including a first and a second bank of cylinders. One example method comprises, adjusting engine injection timing based on a first temperature of a first intake of the first bank and a second temperature of a second intake of the second bank.
摘要翻译：提供了用于操作包括第一和第二气缸组的发动机的方法和系统。一个示例性方法包括：基于第一组的第一摄入的第一温度和第二组的第二摄入的第二温度来调节发动机喷射正时。

6. 发明申请

US20070012294A1 Common fuel rail fuel system for locomotive engine 有权
标题翻译：机车发动机常用燃油轨燃油系统
公开(公告)号：US20070012294A1
公开(公告)日：2007-01-18
申请号：US11181655
申请日：2005-07-14
申请人： Jose Casabianca , Shawn Gallagher , Eric Dillen
发明人： Jose Casabianca , Shawn Gallagher , Eric Dillen
IPC分类号： F02M69/46
CPC分类号： F02M63/027 , F02B2075/1848 , F02D2041/3881 , F02M55/025 , F02M59/102 , F02M63/0205 , F02M63/0225 , F02M63/0295
摘要： A common rail fuel injection apparatus (1) for a multi-bank, diesel locomotive engine (10). A common rail (20,22) is disposed proximate each bank (20,22) of cylinders (12) of the engine to provide high pressure fuel (30) to a fuel flow control apparatus (14) associated with each respective cylinder. A plurality of high-pressure fuel pumps (34) provides high-pressure fuel to at least one of the common rails. A fluid cross connection (38) is provided to convey high pressure fuel between the two common rails, thereby providing for the continued delivery of fuel to all cylinders in the event of a failure of one of the high pressure pumps. The high-pressure pumps are motivated by fuel lobes (64) located on camshaft sections (50a, 50b, 50c) adjoined at a gear driven end 58 of the camshaft (50). Camshaft sections (50d, 50e, 50f) adjoined at an idler end (60) of the camshaft carry lower torque loads than those sections having fuel lobes and may be formed from a lower strength material or may have a smaller shaft diameter.
摘要翻译：一种用于多排柴油机车发动机（10）的共轨燃料喷射装置（1）。公共轨道（20,22）设置在发动机的气缸（12）的每个排（20,22）附近，以向与每个相应气缸相关联的燃料流量控制装置（14）提供高压燃料（30）。多个高压燃料泵（34）向至少一个公共轨道提供高压燃料。提供流体交叉连接（38）以在两个公共轨道之间传送高压燃料，从而在高压泵之一发生故障的情况下提供燃料持续输送到所有气缸。高压泵由位于凸轮轴（50）的齿轮驱动端58处的凸轮轴部分（50a，50b，50c）上的燃料凸起（64）驱动。在凸轮轴的惰轮端（60）处邻接的凸轮轴部分（50d，50e，50f）承载比具有燃料凸角的部分更低的扭矩负载，并且可以由较低强度材料形成或者可以具有较小的轴直径。

7. 发明申请

US20060057974A1 System and method of transmitting data from an aircraft 有权
公开(公告)号：US20060057974A1
公开(公告)日：2006-03-16
申请号：US10942630
申请日：2004-09-16
申请人： James Ziarno , Shawn Gallagher
发明人： James Ziarno , Shawn Gallagher
IPC分类号： H04B1/02 , G06F7/00
CPC分类号： H04B1/3822 , H01Q1/28 , H04B7/18506 , H04L67/12
摘要： A system and method of transmitting data from an aircraft includes a PC card that acquires aircraft data and transmits the aircraft data over a radio frequency communications signal into the skin of the aircraft, which radiates the radio frequency communications signal to a location remote from the aircraft.

8. 发明申请

US20050182554A1 Cam sensor elimination in compression-ignition engines 有权
标题翻译：压缩点火发动机中的凸轮传感器消除
公开(公告)号：US20050182554A1
公开(公告)日：2005-08-18
申请号：US11098975
申请日：2005-04-05
申请人： Vincent Dunsworth , Shawn Gallagher , James Mischler
发明人： Vincent Dunsworth , Shawn Gallagher , James Mischler
IPC分类号： F02D41/00 , F02D41/06 , F02D41/34 , F02D41/40 , F02D41/30
CPC分类号： F02D41/009 , F02D41/008 , F02D41/062 , F02D41/402 , F02D2041/0092
摘要： A method for controlling start of a compression ignition engine having a plurality of cylinders is provided without a cam sensor is provided. Each cylinder includes a respective piston reciprocally movable between respective top and bottom positions along a cylinder longitudinal axis. The method comprises providing a respective fuel delivery assembly for each cylinder. In one embodiment the method further comprises retrieving from memory a set of fuel delivery assembly firing rules and then processing the firing rules so that a firing signal is delivered to each fuel delivery assembly on every crank revolution during a cranking mode of operation. The fuel delivery assembly is arranged to be responsive to any firing signal received during an injection window leading to the top position along the longitudinal axis so as to supply fuel to each cylinder during that injection window. The fuel delivery assembly is further arranged to be insensitive to any firing signal received during an exhaust stroke leading to the top position along said longitudinal axis so that no fuel is delivered to each cylinder during that exhaust stroke.
摘要翻译：提供了一种用于控制具有多个气缸的压燃式发动机起动的方法，而不设置凸轮传感器。每个气缸包括相应的活塞，该活塞可沿气缸纵向轴线在相应的顶部和底部位置之间往复运动。该方法包括为每个气缸提供相应的燃料输送组件。在一个实施例中，该方法还包括从存储器中取回一组燃料输送组件点火规则，然后处理点火规则，使得在起动操作模式期间，在每个曲柄转动时将点火信号传送到每个燃料输送组件。燃料输送组件被布置成响应于在喷射窗口期间接收的任何点火信号，其导致沿着纵向轴线的顶部位置，以便在该喷射窗口期间向每个气缸供应燃料。燃料输送组件还被布置成对于在排气冲程期间接收到的任何点火信号不敏感，导致沿着所述纵向轴线的顶部位置，使得在该排气冲程期间没有燃料被输送到每个气缸。

9. 发明申请

US20050010355A1 CAM SENSOR ELIMINATION IN COMPRESSION-IGNITION ENGINES 失效
标题翻译：压缩点火机中的CAM传感器消除
公开(公告)号：US20050010355A1
公开(公告)日：2005-01-13
申请号：US10615439
申请日：2003-07-08
申请人： Vincent Dunsworth , Shawn Gallagher , James Mischler
发明人： Vincent Dunsworth , Shawn Gallagher , James Mischler
IPC分类号： F02D41/00 , F02D41/06 , F02D41/34 , F02D41/40
CPC分类号： F02D41/009 , F02D41/008 , F02D41/062 , F02D41/402 , F02D2041/0092
摘要： A method for controlling start of a compression ignition engine having a plurality of cylinders without a cam sensor is provided. Each cylinder includes a respective piston reciprocally movable between respective top and bottom positions along a cylinder longitudinal axis. The method comprises providing a respective fuel delivery assembly for each cylinder. In one embodiment the method further comprises retrieving from memory a set of fuel delivery assembly firing rules and then processing the firing rules so that a firing signal is delivered to each fuel delivery assembly on every crank revolution during a cranking mode of operation. The fuel delivery assembly is arranged to be responsive to any firing signal received during an injection window leading to the top position along the longitudinal axis so as to supply fuel to each cylinder during that injection window. The fuel delivery assembly is further arranged to be insensitive to any firing signal received during an exhaust stroke leading to the top position along said longitudinal axis so that no fuel is delivered to each cylinder during that exhaust stroke.
摘要翻译：提供了一种用于控制具有多个没有凸轮传感器的气缸的压燃式发动机起动的方法。每个气缸包括相应的活塞，该活塞可沿气缸纵向轴线在相应的顶部和底部位置之间往复运动。该方法包括为每个气缸提供相应的燃料输送组件。在一个实施例中，该方法还包括从存储器中取回一组燃料输送组件点火规则，然后处理点火规则，使得在起动操作模式期间，在每个曲柄转动时将点火信号传送到每个燃料输送组件。燃料输送组件被布置成响应于在喷射窗口期间接收的任何点火信号，其导致沿着纵向轴线的顶部位置，以便在该喷射窗口期间向每个气缸供应燃料。燃料输送组件还被布置成对于在排气冲程期间接收到的任何点火信号不敏感，导致沿着所述纵向轴线的顶部位置，使得在该排气冲程期间没有燃料被输送到每个气缸。

10. 发明申请

US20050007304A1 Burst pulse circuit for signal lights and method 有权
标题翻译：脉冲脉冲电路用于信号灯和方法
公开(公告)号：US20050007304A1
公开(公告)日：2005-01-13
申请号：US10617280
申请日：2003-07-10
申请人： Shawn Gallagher , Matthew Johnson , Timothy Zink
发明人： Shawn Gallagher , Matthew Johnson , Timothy Zink
IPC分类号： H05B33/08 , G09G3/00
CPC分类号： H05B33/0842 , H05B33/0803 , H05B33/0818
摘要： A circuit is provided for over-driving a super-luminescent light emitting diode having a maximum forward continuous current rating. A power supply provides a pulse width modulated signal to an analog memory connected to the power supply and a pulse generator. The pulse generator includes a window comparator engaged with the analog memory, and is responsive to a portion of the pulse width modulated signal. A power driver that is controlled by the output of the pulse generator, is operably connected with the super-luminescent light emitting diode and with the power supply so as to energize the super-luminescent light emitting diode with a current that is above the maximum forward continuous current rating by between two and ten times that rated current. A signal is also provided along with a method of over-driving a super-luminescent light emitting diode.
摘要翻译：提供一种用于过驱动具有最大正向连续电流额定值的超发光发光二极管的电路。电源向连接到电源的模拟存储器和脉冲发生器提供脉宽调制信号。脉冲发生器包括与模拟存储器接合的窗口比较器，并且响应脉冲宽度调制信号的一部分。由脉冲发生器的输出控制的功率驱动器与超发光发光二极管和电源可操作地连接，以便以超过最大向前的电流激励超发光发光二极管连续额定电流为额定电流的两倍和十倍。还提供了一种过驱动超发光发光二极管的方法。

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