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1. 发明申请

US20120122165A1 RECOMBINANT MICROORGANISM AND METHOD FOR PRODUCING ALIPHATIC POLYESTER WITH THE USE OF THE SAME 审中-公开
标题翻译：重组微生物及其使用生产聚氨酯的方法
公开(公告)号：US20120122165A1
公开(公告)日：2012-05-17
申请号：US13387271
申请日：2010-07-27
申请人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
发明人： Shusei Obata , Masayoshi Muramatsu , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda
IPC分类号： C12P7/62 , C12N1/21
CPC分类号： C12N9/13 , C12N9/1029 , C12P7/62
摘要： This invention provides a recombinant microorganism exhibiting excellent aliphatic polyester productivity and a method for producing an aliphatic polyester with the use of the recombinant microorganism. In this invention, the Megasphaera elsdenii-derived pct gene (and/or the Staphylococcus aureus-derived pct gene) and the Pseudomonas sp. 61-3 strain-derived PHA synthase gene (phaC2 gene) (and/or the Alcanivorax borkumensis SK2 strain-derived PHA synthase gene) are introduced into a host microorganism.
摘要翻译：本发明提供使用重组微生物显示优异脂肪族聚酯生产率的重组微生物和生产脂肪族聚酯的方法。在本发明中，来自大疱疹病毒的巨噬细胞源基因（和/或金黄色葡萄球菌来源的pct基因）和假单胞菌属（Pseudomonas sp。 61-3菌株衍生的PHA合酶基因（phaC2基因）（和/或阿尔卡氏菌属Bclumum SK2株衍生的PHA合酶基因）引入宿主微生物中。

2. 发明授权

US08748137B2 Method for production of polylactate using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚乳酸的方法
公开(公告)号：US08748137B2
公开(公告)日：2014-06-10
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

3. 发明申请

US20110212497A1 METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚乙烯的方法
公开(公告)号：US20110212497A1
公开(公告)日：2011-09-01
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N1/00
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

4. 发明授权

US08563281B2 Method for production of polyester copolymer using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US08563281B2
公开(公告)日：2013-10-22
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/20 , C12N1/00
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

5. 发明申请

US20110104768A1 METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US20110104768A1
公开(公告)日：2011-05-05
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/00 , C12N1/21
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

6. 发明申请

US20200283804A1 NOVEL ALDEHYDE SYNTHASE GENE, RECOMBINANT MICROORGANISM COMPRISING THE SAME, AND METHOD FOR PRODUCING ALKANE USING THE SAME 审中-公开
公开(公告)号：US20200283804A1
公开(公告)日：2020-09-10
申请号：US16807495
申请日：2020-03-03
申请人： Masayoshi Muramatsu , Shusei Obata , Masakazu Ito , Jun Ogawa , Shigenobu Kishino
发明人： Masayoshi Muramatsu , Shusei Obata , Masakazu Ito , Jun Ogawa , Shigenobu Kishino
IPC分类号： C12P5/02 , C12N9/02
摘要： The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

7. 发明授权

US10894967B2 Aldehyde synthase gene, recombinant microorganism comprising the same, and method for producing alkane using the same 有权
公开(公告)号：US10894967B2
公开(公告)日：2021-01-19
申请号：US16807495
申请日：2020-03-03
申请人： Masayoshi Muramatsu , Shusei Obata , Masakazu Ito , Jun Ogawa , Shigenobu Kishino
发明人： Masayoshi Muramatsu , Shusei Obata , Masakazu Ito , Jun Ogawa , Shigenobu Kishino
IPC分类号： C12N9/02 , C12P5/02 , C12R1/19 , C12R1/22
摘要： The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.

8. 发明授权

US06974685B2 High production method of prenyl alcohol using saccharomyces cells 失效
标题翻译：使用酵母细胞制备异戊醇的高生产方法
公开(公告)号：US06974685B2
公开(公告)日：2005-12-13
申请号：US10022434
申请日：2001-12-20
申请人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
发明人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
IPC分类号： C12N1/38 , C12P7/04 , C12R1/645 , C12P7/02
CPC分类号： C12N1/38 , C12P7/04
摘要： The present invention provides a high production method of prenyl alcohol, which comprises culturing prenyl alcohol-producing cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene to produce and accumulate prenyl alcohol in the cells; allowing the accumulated prenyl alcohol to be secreted from the cells; and then collecting prenyl alcohol. The present invention enables prenyl alcohol to be highly produced in and effectively secreted from prenyl alcohol-producing cells by culturing the cells in a medium with an increased sugar content in the presence of at least one member selected from the group consisting of a surfactant, a fat or oil, and a terpene.
摘要翻译：本发明提供了高产的异戊醇的制备方法，其包括在选自表面活性剂，脂肪或油中的至少一种的存在下，在具有增加的糖含量的培养基中培养异戊醇产生细胞，和萜烯在细胞中产生和积累异戊醇; 允许从细胞中分泌积累的异戊醇; 然后收集异戊醇。本发明通过在选自表面活性剂，表面活性剂，表面活性剂的至少一种的存在下，在具有增加的糖含量的介质中培养细胞，使异戊烯醇高产生并由异戊烯醇产生细胞有效分泌脂肪或油，和萜烯。

9. 发明授权

US06972191B2 Method for production of geranylgeraniol and analogous compounds thereof by microorganisms 失效
标题翻译：通过微生物生产香叶基香叶醇及其类似物的方法
公开(公告)号：US06972191B2
公开(公告)日：2005-12-06
申请号：US10022695
申请日：2001-12-20
申请人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
发明人： Masayoshi Muramatsu , Shusei Obata , Sakayu Shimizu
IPC分类号： C07C33/02 , C12P7/04 , C12R1/85 , C12P7/02
CPC分类号： C12P7/04
摘要： The present invention provides a method for producing geranylgeraniol and analogous compounds thereof, which comprises culturing yeast cells (ascomycetes and deuteromycetes), bacterial cells, actinomycete cells or filamentous fungus cells, all of which are capable of producing geranylgeraniol and analogous compounds thereof, in a medium to produce and accumulate geranylgeraniol and analogous compounds thereof in the cells and/or in the extracellular environment; and then collecting these compounds. The present invention enables inexpensive mass production of geranylgeraniol and analogous compounds thereof by using microorganisms capable of producing geranylgeraniol, farnesol and/or nerolidol useful as biosynthetic intermediates of terpenes, carotenoids and/or steroids.
摘要翻译：本发明提供了一种生产香叶基香叶醇及其类似化合物的方法，其包括培养酵母细胞（子囊菌和氘核菌素），细菌细胞，放线菌细胞或丝状真菌细胞，所有这些能够产生香叶基香叶醇及其类似物培养基以在细胞和/或细胞外环境中产生和积累香叶基香叶醇及其类似物; 然后收集这些化合物。本发明可以通过使用能够产生香叶基香叶醇，法呢醇和/或橙花叔醇作为萜烯，类胡萝卜素和/或类固醇的生物合成中间体的微生物，从而廉价地批量生产香叶基香叶醇及其类似物。

10. 发明授权

US09005953B2 Recombinant microorganism having butanol production capacity and butanol production method 有权
标题翻译：具有丁醇生产能力的重组微生物和丁醇生产方法
公开(公告)号：US09005953B2
公开(公告)日：2015-04-14
申请号：US12775048
申请日：2010-05-06
申请人： Masayoshi Muramatsu , Shusei Obata , Satoshi Yoneda , Masahiro Sugimura , Tomohisa Kuzuyama
发明人： Masayoshi Muramatsu , Shusei Obata , Satoshi Yoneda , Masahiro Sugimura , Tomohisa Kuzuyama
IPC分类号： C12N1/21 , C12P7/16 , C12N9/02 , C12N9/10
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/1029 , Y02E50/10
摘要： This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism.
摘要翻译：本发明涉及具有改善的丁醇生产能力和使用这种重组微生物的丁醇生产的重组微生物，其效率很高。在本发明中，编码能够从丙二酰辅酶A和乙酰辅酶A合成乙酰乙酰辅酶A的酶的乙酰乙酰辅酶A合成酶基因以及能够从乙酰乙酰辅酶A合成丁醇的丁醇生物合成基因的一组被引入宿主微生物。

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