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1. 发明申请

US20110104768A1 METHOD FOR PRODUCTION OF POLYESTER COPOLYMER USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US20110104768A1
公开(公告)日：2011-05-05
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsuhiro Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/00 , C12N1/21
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3HB and LA via microbial fermentation with the use of a sugar as a starting material.The following is provided: a method for producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate, a protein capable of catalyzing a reaction of forming acetoacetyl-CoA from two acetyl-CoA molecules, a protein capable of catalyzing a reaction of acetoacetyl-CoA reduction, and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of at least one amino acid other than the amino acids at positions 130, 325, 477, and 481 or by insertion of at least one amino acid residue; and (2) collecting the polyester copolymer from the culture product obtained in step (1). According to the production method of the present invention, a polyester copolymer consisting of 3HB and LA can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种利用糖作为原料，通过微生物发酵有效地制备由3HB和LA组成的聚酯共聚物的方法。提供以下：由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的制造方法，其特征在于，包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸盐，能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的反应的蛋白质，能够催化乙酰乙酰辅酶A还原反应的蛋白质，以及能够催化由下列氨基组成的聚羟基链烷酸酯合成反应的蛋白质含有碳源的培养基中的酸序列（a）或（b）：（a）从SEQ ID NO：2所示的氨基酸序列衍生的氨基酸序列，通过将位置130的至少一个氨基酸置换，325,477和481具有不同的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的至少一个氨基酸的另外缺失或取代或通过插入至少一个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚酯共聚物。根据本发明的制造方法，可以使用廉价的碳源作为原料，有效地制造由3HB和LA组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

2. 发明授权

US08563281B2 Method for production of polyester copolymer using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚酯共聚物的方法
公开(公告)号：US08563281B2
公开(公告)日：2013-10-22
申请号：US12989333
申请日：2009-04-23
申请人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
发明人： Seiichi Taguchi , Kenji Tajima , Yasuharu Satoh , Ken'ichiro Matsumoto , Miwa Yamada , Shusei Obata , Hiromi Kambe , Katsunori Kohda , Katsuhiro Ohno , Takashi Shimamura
IPC分类号： C12P7/62 , C12N1/20 , C12N1/00
CPC分类号： C12P7/62
摘要： The object of the present invention is to provide a method for efficiently producing a polyester copolymer consisting of 3-hydroxybutyrate and lactate via microbial fermentation with the use of a sugar as a starting material. The method may comprise culturing a recombinant microorganism expressing: a protein capable of catalyzing the transfer of CoA to propionic acid and/or lactate; a protein capable of catalyzing the formation of acetoacetyl-CoA from two acetyl-CoA molecules; a protein capable of catalyzing acetoacetyl-CoA reduction; and a protein capable of catalyzing polyhydroxyalkanoate synthesis. According to the production method, a polyester copolymer consisting of 3-hydroxybutyrate and lactate can be efficiently produced using an inexpensive carbon source as a starting material, and thus the production cost of a biodegradable plastic can be reduced.
摘要翻译：本发明的目的是提供一种通过使用糖作为原料通过微生物发酵有效地制备由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物的方法。该方法可以包括培养表达：能够催化CoA转移到丙酸和/或乳酸盐的蛋白质的重组微生物; 能够催化从两个乙酰辅酶A分子形成乙酰乙酰辅酶A的蛋白质; 能够催化乙酰乙酰辅酶A还原的蛋白质; 和能够催化聚羟基链烷酸酯合成的蛋白质。根据该制造方法，可以使用廉价的碳源作为起始材料有效地制造由3-羟基丁酸酯和乳酸酯组成的聚酯共聚物，从而可以降低生物降解性塑料的制造成本。

3. 发明申请

US20110212497A1 METHOD FOR PRODUCTION OF POLYLACTATE USING RECOMBINANT MICROORGANISM 有权
标题翻译：使用重组微生物生产聚乙烯的方法
公开(公告)号：US20110212497A1
公开(公告)日：2011-09-01
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N1/00
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

4. 发明授权

US08748137B2 Method for production of polylactate using recombinant microorganism 有权
标题翻译：使用重组微生物生产聚乳酸的方法
公开(公告)号：US08748137B2
公开(公告)日：2014-06-10
申请号：US13126157
申请日：2009-10-27
申请人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
发明人： Shusei Obata , Hiromi Kambe , Masakazu Ito , Takashi Shimamura , Katsunori Kohda , Seiichi Taguchi
IPC分类号： C12P7/62 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/62 , C12N9/1029
摘要： It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.The method is a method for producing a polylactate, which comprises the steps of: (1) culturing a recombinant microorganism having a protein capable of catalyzing a reaction of transferring CoA to propionic acid and/or lactate and a protein capable of catalyzing a reaction of polyhydroxyalkanoate synthesis consisting of the following amino acid sequence (a) or (b) in a medium containing a carbon source: (a) an amino acid sequence derived from the amino acid sequence shown in SEQ ID NO: 2 by substitution of at least one of the amino acids at positions 130, 325, 477, and 481 with different amino acid(s); or (b) an amino acid sequence derived from the protein specified in (a) by additional deletion or substitution of one or several amino acid(s) other than the amino acids at positions 130, 325, 477, and 481 or by insertion of one or several amino acid residue(s); and (2) collecting the polylactate from the culture product obtained in step (1).
摘要翻译：本发明的目的是提供一种利用糖作为起始原料通过微生物发酵有效生产聚乳酸的方法。该方法是制备聚乳酸的方法，其包括以下步骤：（1）培养具有能够催化将CoA转移到丙酸和/或乳酸的反应的蛋白质的蛋白质和能够催化反应的蛋白质的重组微生物在含有碳源的培养基中由以下氨基酸序列（a）或（b）组成的聚羟基链烷酸酯合成物：（a）通过至少一个取代至少一个氨基酸序列的氨基酸序列，所述氨基酸序列来自SEQ ID NO：2所示的氨基酸序列的具有不同氨基酸的位置130,325,477和481处的氨基酸; 或（b）衍生自（a）中规定的蛋白质的氨基酸序列，通过除了位置130,325,477和481之外的氨基酸以外的一个或几个氨基酸的另外缺失或取代，或通过插入一个或几个氨基酸残基; 和（2）从步骤（1）获得的培养产物中收集聚乳酸。

5. 发明申请

US20090226988A1 Method for producing polymer 审中-公开
标题翻译：生产聚合物的方法
公开(公告)号：US20090226988A1
公开(公告)日：2009-09-10
申请号：US12291779
申请日：2008-11-13
申请人： Kenji Tajima , Yasuharu Satou , Masanobu Munekata , Seiichi Taguchi , Tokuo Matsushima
发明人： Kenji Tajima , Yasuharu Satou , Masanobu Munekata , Seiichi Taguchi , Tokuo Matsushima
IPC分类号： C12P7/62 , C08G63/02
CPC分类号： C12P7/625
摘要： A method for producing a polymer including a chemical thioester exchange reaction for forming an acetyl-CoA by reacting an acetyl-thioester with CoA, a monomer-producing reaction for forming a (monomer precursor)-CoA derivative by reacting at least one monomer precursor compound with the acetyl-CoA and a polymerization reaction for forming the polymer comprising units of the monomer by polymerizing the (monomer precursor)-CoA derivative.
摘要翻译：一种制备聚合物的方法，所述聚合物包括通过使乙酰基 - 硫酯与CoA反应形成乙酰辅酶A的化学硫酯交换反应，用于形成（单体前体）-CoA衍生物的单体制备反应，使至少一种单体前体化合物与乙酰辅酶A和通过聚合（单体前体）-CoA衍生物形成包含单体单元的聚合物的聚合反应。

6. 发明授权

US5302989A Method of projecting stereoscopic image and apparatus for printing stereoscopic photograph 失效
标题翻译：突出立体图像的方法和打印立体照片的装置
公开(公告)号：US5302989A
公开(公告)日：1994-04-12
申请号：US955061
申请日：1992-10-01
申请人： Seiichi Taguchi , Samon Hosoya , Shunkichi Igarashi
发明人： Seiichi Taguchi , Samon Hosoya , Shunkichi Igarashi
IPC分类号： G03B35/00 , G03B35/08 , G03B35/14 , G03B35/24
CPC分类号： G03B35/14 , G03B35/24
摘要： In the improved method for projection a stereoscopic image, an additional lenticular sheet that has an appropriate level difference and a different pitch of lenticular lenses than a lenticular recording material such as a lenticular light-sensitive material is either placed directly on top of said lenticular recording material or inserted above and in the vicinity of said lenticular recording material, with said additional lenticular sheet being preferably moved either in one direction or reciprocally in a direction generally perpendicular to its own generatrix. This method can be implemented with the improved apparatus for printing a stereoscopic photograph. Using the improved method and apparatus, the development of moire can be suppressed and, at the same time, the line width of linear image elements to be recorded can be easily expanded.
摘要翻译：在用于投影立体图像的改进方法中，具有与透镜状记录材料（例如透镜感光材料）相比的具有适当水平差和双凸透镜的不同节距的附加透镜片或被直接放置在所述透镜记录的顶部材料或插入所述透镜状记录材料的上方和附近，其中所述附加的双凸透镜片优选地在一个方向上或在大致垂直于其自身母线的方向上往复移动。该方法可以用用于打印立体照片的改进的装置来实现。使用改进的方法和装置，可以抑制莫尔条纹的发展，并且同时可以容易地扩展要记录的线性图像元素的线宽。

7. 发明授权

US5539487A Method and apparatus for recording stereoscopic images and lenticular recording material used therefor 失效
标题翻译：用于记录立体图像和用于其的透镜记录材料的方法和装置
公开(公告)号：US5539487A
公开(公告)日：1996-07-23
申请号：US24761
申请日：1993-03-02
申请人： Seiichi Taguchi , Shunkichi Igarashi
发明人： Seiichi Taguchi , Shunkichi Igarashi
IPC分类号： G03B35/00 , G02B3/00 , G02B26/12 , G02B27/22 , G03B35/24 , G03C9/00 , G03C9/08 , H04N13/00 , H04N13/04 , G03B35/08
CPC分类号： G02B3/005 , G02B26/123 , G02B27/2214 , G03B35/24 , H04N13/0055 , H04N13/0221 , H04N13/0228 , H04N13/0282 , H04N13/0025 , H04N13/0051 , H04N13/0059 , H04N13/0257 , H04N13/0285 , H04N13/0296 , H04N13/0404 , H04N13/0422 , H04N13/0445 , H04N13/0459
摘要： The improved method and apparatus for recording a stereoscopic image read a plurality of original images photoelectrically, split each original image into linear images of a desired pitch, perform any necessary image processing operations such as arranging the linear images in the recording order, and record the linear images by scan exposure on a lenticular light-sensitive material having a lenticular sheet and a recording layer. The method and apparatus permit the lenticular light-sensitive material to be scan exposed from the rear side and they also enable the linear images to be recorded with increased line width and a stereoscopic image to be formed with satisfactory registration between the principal objects of the original images. The improved lenticular light-sensitive material has an anti-halation layer provided between the lenticular sheet and the recording layer, thus permitting scan exposure to be effected from the rear side. According to the present invention, stereoscopic images of high quality that have no perceivable unnaturalness such as flickers can be recorded with great ease and at any desired magnification.
摘要翻译：用于记录立体图像的改进方法和装置以光电方式读取多个原始图像，将每个原始图像分割为期望间距的线性图像，执行任何必要的图像处理操作，例如以记录顺序布置线性图像，并且记录通过在具有透镜片和记录层的透镜感光材料上进行扫描曝光的线性图像。该方法和装置允许双凸透镜感光材料从后侧扫描曝光，并且还使线性图像能够以增加的线宽被记录，并且立体图像将被形成为具有令人满意的原始对象之间的对准图片。改进的透镜感光材料在双凸透镜片和记录层之间具有防晕层，从而允许从背面进行扫描曝光。根据本发明，可以非常容易地并且以任何期望的放大率来记录不具有可感知的不自然的诸如闪烁的高质量的立体图像。

8. 发明授权

US5349419A Method and apparatus for recording stereoscopic images 失效
标题翻译：用于记录立体图像的方法和装置
公开(公告)号：US5349419A
公开(公告)日：1994-09-20
申请号：US046838
申请日：1993-04-14
申请人： Seiichi Taguchi , Shunkichi Igarashi
发明人： Seiichi Taguchi , Shunkichi Igarashi
IPC分类号： G03B35/00 , G03B35/08 , G03B35/14 , G03C9/08 , H04N1/23 , H04N13/00 , G03B27/32
CPC分类号： H04N13/0055 , G03B35/14 , H04N1/00201 , H04N1/23 , H04N13/0239 , H04N13/0003 , H04N13/0037 , H04N13/0051 , H04N13/0228 , H04N13/0404
摘要： The improved method and apparatus for recording a stereoscopic image calculate the linear image recording positions for each lenticular lens in connection with all of the linear images of the nth original image in such a way that all of the linear images of the nth original image that are to be recorded in association with the respective lenticular lenses will fall within the same area at the intended viewing position, and record the linear images by an image forming means on the basis of the results of said calculation. Therefore, according to the present invention, stereoscopic images of high quality can be recorded using the lenticular sheet without causing any serious defects such as multiple image or image flickering.
摘要翻译：用于记录立体图像的改进方法和装置结合第n个原始图像的所有线性图像计算每个双凸透镜的线性图像记录位置，使得第n个原始图像的所有线性图像与相应的双凸透镜相关联地记录的记录将落在预期观看位置的相同区域内，并且基于所述计算的结果，通过图像形成装置记录线性图像。因此，根据本发明，可以使用透镜片来记录高品质的立体图像，而不会引起多重图像或图像闪烁等严重缺陷。

9. 发明授权

US4851327A Photographic color photosensitive material with two layer reflective support 失效
标题翻译：摄影彩色感光材料，双层反光支架
公开(公告)号：US4851327A
公开(公告)日：1989-07-25
申请号：US73977
申请日：1987-07-15
申请人： Tetsuro Fuchizawa , Masaru Sano , Seiichi Taguchi , Keisuke Shiba , Tadashi Ogawa
发明人： Tetsuro Fuchizawa , Masaru Sano , Seiichi Taguchi , Keisuke Shiba , Tadashi Ogawa
IPC分类号： G03C1/76
CPC分类号： G03C1/76
摘要： The present invention relates to a photographic support having a secondary diffuse-reflective surface, and a color photosensitive material comprising at least one silver halide emulsion layer formed on the secondary diffuse-reflective surface of a photographic support. A photographic support of the present invention provides an image having remarkably improved brightness, saturation, tone reproducibility in shadow areas and sharpness. A color photosensitive material of the present invention provides an image having remakably improved brightness, saturation, tone reproducibility in shades and sharpness and also capable of rapid development.
摘要翻译：本发明涉及一种具有次级漫反射表面的照相支架，以及一种彩色光敏材料，其包括形成在照相支撑体的次级漫反射表面上的至少一个卤化银乳剂层。本发明的摄影载体提供了一种具有显着提高的亮度，饱和度，阴影区域中的色调再现性和清晰度的图像。本发明的彩色感光材料提供了具有可改善的亮度，饱和度，色调和色调的再现性以及清晰度并且还能够快速显影的图像。

10. 发明申请

US20100143970A1 TRANSGLUTAMINASE HAVING DISULFIDE BOND INTRODUCED THEREIN 有权
标题翻译：具有衍生物的衍生蛋白酶的转移蛋白酶
公开(公告)号：US20100143970A1
公开(公告)日：2010-06-10
申请号：US12541220
申请日：2009-08-14
申请人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
发明人： Keiichi Yokoyama , Mototaka Suzuki , Tatsuki Kashiwagi , Eiichiro Suzuki , Masayo Date , Seiichi Taguchi
IPC分类号： C12P21/00 , C12N9/10 , C07H21/00 , C12N15/74 , C12N1/21
CPC分类号： C12N9/1044 , C12Y203/02013
摘要： A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).
摘要翻译：突变以具有改善的耐热性和/或pH稳定性的转谷氨酰胺酶蛋白。在能够形成二硫键的半胱氨酸残基（SS键）上引入WT转谷氨酰胺酶的突变。

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