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1. 发明申请

US20080087547A1 Electrophoresis apparatus 失效
标题翻译：电泳仪
公开(公告)号：US20080087547A1
公开(公告)日：2008-04-17
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/447 , G01N21/64
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译：为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。

2. 发明授权

US07655125B2 Electrophoresis apparatus 失效
标题翻译：电泳仪
公开(公告)号：US07655125B2
公开(公告)日：2010-02-02
申请号：US11905539
申请日：2007-10-02
申请人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
发明人： Shin Nakamura , Rintaro Yamamoto , Akira Harada , Makoto Hazama , Takashi Ikegami , Toru Kaji , Naoya Endo , Hidesato Kumagai , Tetsuo Ohashi , Atsushi Inami , Keisuke Miyamoto , Shinichi Utsunomiya
IPC分类号： G01N27/453
CPC分类号： G01N27/44791 , G01N27/44721 , G01N27/44743 , G01N27/44782
摘要： For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.
摘要翻译：为了使电泳装置的操作自动化并提高通过量，本电泳装置具有能够控制放置在其上的电泳板的温度的两个压板，用于在压力下发送加载介质的装载介质充电单元，具有连接到装载介质充电单元的一对喷嘴，用于分配样品以对放置在压板上的电泳板的分配开口进行采样的移液器机构，用于存储样品板的堆垛机构，装载缓冲溶液供应机构，装载缓冲溶液连接到装载缓冲溶液供应机构的分配机构，用于允许放置在台板上的每个电泳板电泳分离的动力单元，以及用于光学检测通过电泳板的每个电泳流路移动的组分的检测器。

3. 发明申请

US20060201809A1 Sample injection method using capillary plate 审中-公开
标题翻译：采用毛细管样品注射法
公开(公告)号：US20060201809A1
公开(公告)日：2006-09-14
申请号：US11369814
申请日：2006-03-08
申请人： Naoya Endo , Tetsuo Ohashi , Shin Nakamura
发明人： Naoya Endo , Tetsuo Ohashi , Shin Nakamura
IPC分类号： C07K1/26
CPC分类号： C07K1/26
摘要： In a sample injection method to a capillary plate, a first liquid is provided into a large-capacity reservoir and a small-capacity reservoir formed at a bottom of the large-capacity reservoir. A sample is dissolved in a second liquid having heavier specific gravity than the first liquid, and the sample dissolved in the second liquid is injected through the first liquid into the small-capacity reservoir.
摘要翻译：在向毛细板的样品注入方法中，将第一液体提供到大容量储存器和形成在大容量储存器的底部的小容量储存器。将样品溶解在比第一液体重的比重的第二液体中，溶解在第二液体中的样品通过第一液体注入小容量储存器。

4. 发明申请

US20080257734A1 Method for analyzing analytical sample in high-throughput type automatic analytical device 审中-公开
标题翻译：高通量型自动分析装置分析样品分析方法
公开(公告)号：US20080257734A1
公开(公告)日：2008-10-23
申请号：US12078820
申请日：2008-04-07
申请人： Tetsuo Ohashi , Shin Nakamura
发明人： Tetsuo Ohashi , Shin Nakamura
IPC分类号： G01N27/26
CPC分类号： G01N27/44747
摘要： In a method for analyzing a sample by using an analytical device having capillary channels, a solvent for the sample is prepared. The solvent includes at least one component selected from the group consisting of polyvalent alcohols, sugars, and hydrophilic polymers. A sample solution is prepared by mixing an analytical sample with the solvent, and the sample solution is analyzed by using the analytical device.
摘要翻译：在通过使用具有毛细通道的分析装置分析样品的方法中，制备用于样品的溶剂。溶剂包括至少一种选自多元醇，糖和亲水性聚合物的组分。通过将分析样品与溶剂混合来制备样品溶液，并使用分析装置分析样品溶液。

5. 发明申请

US20060213775A1 Solvent for preparation of analytical sample 审中-公开
标题翻译：溶剂用于制备分析样品
公开(公告)号：US20060213775A1
公开(公告)日：2006-09-28
申请号：US11386808
申请日：2006-03-23
申请人： Tetsuo Ohashi , Shin Nakamura
发明人： Tetsuo Ohashi , Shin Nakamura
IPC分类号： G01N27/00 , C07K1/26
CPC分类号： G01N27/44747
摘要： A solvent for use in the preparation of an analytical sample includes at least one component selected from the group consisting of polyvalent alcohols, sugars, and hydrophilic polymers, and has a boiling point at one atmosphere of from 101° C. to 300° C. The solvent facilitates the preparation of samples for use in analyzing extremely small quantities in analytical devices having capillary channels. By virtue of its boiling point, the solvent can prevent the concentration of analytical samples without requiring special devices or tools, even when a minute quantity of sample solution is left for a long period of time or is placed in a high-temperature environment.
摘要翻译：用于制备分析样品的溶剂包括至少一种选自多元醇，糖和亲水性聚合物的组分，并且在一个气氛中的沸点为101℃至300℃。溶剂有助于制备样品，用于分析具有毛细管通道的分析装置中极少量的样品。由于其沸点，即使在少量样品溶液长时间放置或放置在高温环境中时，溶剂也可以防止分析样品的浓度而不需要特殊的装置或工具。

6. 发明申请

US20090191594A1 Microchemistry reaction method and device 有权
标题翻译：微化学反应方法及装置
公开(公告)号：US20090191594A1
公开(公告)日：2009-07-30
申请号：US12010443
申请日：2008-01-25
申请人： Tetsuo Ohashi
发明人： Tetsuo Ohashi
IPC分类号： C12P19/34 , B01J19/08
CPC分类号： C12P19/34 , B01J2219/00495 , B01J2219/00527 , B01J2219/00655 , B01L3/5027 , B01L3/502792 , B01L7/525 , B01L2400/043
摘要： A chemical reaction is conducted in a fluid of a droplet inside a reaction receptacle or on a surface of a reaction substrate. Fluctuations of a magnetic field are applied to the droplet including an aqueous solution having magnetic body particles with a hydrophilic surface, and a physical force is transmitted to the surrounding aqueous solution through the magnetic body particles. The droplet is thus moved by the physical force to conduct an operation necessary for a chemical reaction.
摘要翻译：化学反应在反应容器内部或反应基材表面上的液滴中进行。磁场的波动被施加到包括具有亲水表面的磁性体颗粒的水溶液的液滴中，物理力通过磁性体颗粒传递到周围的水溶液。因此，液滴通过物理力移动以进行化学反应所需的操作。

7. 发明授权

US5795717A Oligonucleotides for detecting bacteria and detection process 失效
公开(公告)号：US5795717A
公开(公告)日：1998-08-18
申请号：US328710
申请日：1994-10-25
申请人： Tomoko Nakayama , Jun Tada , Shigeru Fukushima , Tetsuo Ohashi
发明人： Tomoko Nakayama , Jun Tada , Shigeru Fukushima , Tetsuo Ohashi
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/689
摘要： A synthetic oligonucleotide which is complementary to a nucleotide sequence of a gene selected from the group consisting of the Shiga toxin gene of Shigella species, the ipaH gene of Shigella species and EIEC, the invE gene of Shigella species and EIEC, the araC gene of Salmonella species, the Verocytotoxin-1 gene of EHEC or VTEC, the Verocytotoxin-2 gene of EHEC or VTEC, the toxic shock syndrome toxin-1 gene of Staphylococcus aureus, the ctx gene of Vibrio cholerae, and the enterotoxin gene of Clostridium perfringens; a method for detecting a bacterial strain by amplifying a region of the above gene by PCR using the above oligonucleotides as primers and detecting the amplified region; and a kit for the detection of the bacterial strain.

8. 发明授权

US08852868B2 Method for real-time nucleic acid amplification by droplet manipulation 有权
标题翻译：通过液滴操作实时核酸扩增的方法
公开(公告)号：US08852868B2
公开(公告)日：2014-10-07
申请号：US13643986
申请日：2011-02-02
申请人： Tetsuo Ohashi , Masayuki Watanabe
发明人： Tetsuo Ohashi , Masayuki Watanabe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2527/101 , C12Q2527/15 , C12Q2561/113 , C12Q2563/143 , C12Q2563/159
摘要： The present invention provides a real time nucleic acid amplification reaction method comprising performing a nucleic acid amplification reaction in a droplet present in a container. The droplet is composed of a nucleic acid amplification reaction liquid including a nucleic acid to be amplified and magnetic particles. The container holds a droplet encapsulating medium immiscible with the nucleic acid amplification reaction liquid forming the droplet, and has a transport surface having a temperature gradient. Fluorochrome is initially contained in the droplet encapsulating medium, and optionally in the droplet, at start of the nucleic acid amplification reaction. The droplet is transported together with the magnetic particles by generating and applying a magnetic field so that the droplet is placed on the transport surface at a temperature point at which the nucleic acid synthesis reaction is started and maintained, thereby controlling a temperature of the reaction liquid.
摘要翻译：本发明提供了一种实时核酸扩增反应方法，包括在存在于容器中的液滴中进行核酸扩增反应。液滴由包含待扩增的核酸和磁性颗粒的核酸扩增反应液组成。容器容纳与形成液滴的核酸扩增反应液不混溶的液滴封装介质，并具有温度梯度的输送面。在核酸扩增反应开始时，荧光染料最初包含在液滴包封介质中，并且任选地包含在液滴中。通过产生和施加磁场使液滴与磁性颗粒一起输送，使得液滴在开始和维持核酸合成反应的温度点放置在输送表面上，从而控制反应液体的温度。

9. 发明授权

US5683883A Oligonucleotides for detecting Salmonella species and detection process using the same 失效
标题翻译：用于检测沙门氏菌物种的寡核苷酸和使用其的检测过程
公开(公告)号：US5683883A
公开(公告)日：1997-11-04
申请号：US637902
申请日：1996-04-26
申请人： Tetsuo Ohashi , Reiko Tanaka
发明人： Tetsuo Ohashi , Reiko Tanaka
IPC分类号： G01N33/53 , C07H21/04 , C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/42 , G01N33/566 , G01N33/569 , C07H19/00 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/689
摘要： Synthetic oligonucleotides having a nucleotide sequence contained in the enterotoxin genes of Salmonella species; a method for detecting a bacterial strain of Salmonella species in test specimens by amplifying a region of the genes by PCR using the above oligonucleotides as primers; and a kit for the detection of Salmonella species.
摘要翻译：具有包含在沙门氏菌属的肠毒素基因中的核苷酸序列的合成寡核苷酸; 通过使用上述寡核苷酸作为引物，通过PCR扩增基因区域来检测试样中沙门氏菌属细菌菌株的方法; 以及用于检测沙门氏菌物种的试剂盒。

10. 发明授权

US5468852A Oligonucleotides for detecting bacteria 失效
标题翻译：用于检测细菌的寡核苷酸
公开(公告)号：US5468852A
公开(公告)日：1995-11-21
申请号：US932379
申请日：1992-08-19
申请人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
发明人： Tetsuo Ohashi , Jun Tada , Shigeru Fukushima , Hiroko Ozaki , Naoyuki Nishimura , Yoshinari Shirasaki , Koichi Yamagata
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , Y10S435/848 , Y10S435/909
摘要： Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.
摘要翻译：可选择性地与副溶血弧菌特异性基因杂交的寡核苷酸（SEQ ID NO：1-8），与产碱性大肠杆菌线粒体的LT基因选择性杂交的寡核苷酸（SEQ ID NO：9-13），寡核苷酸（SEQ ID NO：14-21）选择性杂交与产毒性大肠杆菌的STh或STp基因，与金黄色葡萄球菌的entA，B，C或D基因选择性杂交的寡核苷酸（SEQ ID NO：22-47）或选择性与下列物质选择性杂交的寡核苷酸（SEQ ID NO：48-53）制备Staplyloccus金黄色葡萄球菌的entE基因并用作基因扩增的引物，从而仅选择性地检测引起食物中毒的相应微生物。

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