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    • 4. 发明授权
    • Method for counting living cells of microbes and apparatus therefor
    • 计数微生物活细胞的方法及其设备
    • US5389544A
    • 1995-02-14
    • US658646
    • 1991-02-21
    • Kiyoshi SugataRyohei UedaTakashi DoiTakashi OnishiKazunori Matsumoto
    • Kiyoshi SugataRyohei UedaTakashi DoiTakashi OnishiKazunori Matsumoto
    • G01N15/10G01N15/14C12M1/34C12M1/00G01N21/00
    • G01N15/1459G01N2015/1486G01N21/6428Y10S435/808Y10S436/80Y10T436/117497
    • A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluorescein, capable of reacting with one or more substances intrinsic of the living cell, such as enzyme, to form an accumulative fluorescent product within the living cells; a reactor inserted in the flow line of the sample and being provided for the reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to the reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.
    • 一种用于在流动样品的同时流动样品时连续测量流体样品中的微生物的活细胞的方法,该装置包括用于以预定速率向样品的流动管线供应诸如荧光素的衍生物的试剂,所述试剂能够 与活细胞固有的一种或多种物质如酶反应,在活细胞内形成累积荧光产物; 插入样品流线中的反应器,用于使试剂与活细胞中的细胞固有物质反应; 光检测系统,其布置在所述反应器之后,用于在通过激发光线照射所述荧光产品时,从漂浮在流动样品中的每个活细胞中的荧光产物中检测作为各个发光点发出的荧光; 以及包括用于计数从发光点的每个荧光产生的电脉冲的脉冲计数器的电子单元。
    • 5. 发明申请
    • Method of liquid detoxification and apparatus therefor
    • 液体解毒方法及其设备
    • US20090078654A1
    • 2009-03-26
    • US12292454
    • 2008-11-19
    • Kazuki NishizawaKazuhiko FujiseMasayuki TabataKiyoshi SugataRyouhei UedaTakashi UedaHirokazu OkadaTomoaki Oomura
    • Kazuki NishizawaKazuhiko FujiseMasayuki TabataKiyoshi SugataRyouhei UedaTakashi UedaHirokazu OkadaTomoaki Oomura
    • C02F1/467C02F1/76C02F103/08C02F1/34
    • B63J4/002B63B35/44C02F1/28C02F1/34C02F1/38C02F1/4674C02F2103/008Y02W10/37
    • Conversion of an untreated liquid to a clean harmless treated liquid through microbe removal, characterized in that a mechanical treatment for damaging microbes present in a liquid to thereby effect extinction thereof and sterilization, combined with a chlorination in which a chlorine-containing substance is formed from a liquid and injected into a liquid to thereby effect microbe extinction and sterilization, is applied to a liquid. There is further provided an electrolytic circulation system comprising applying a detoxification treatment for extinction of microbes in seawater and sterilization by means of detoxification facilities on land or on the sea to seawater introduced through a seawater introduction channel and accommodating the seawater having been thus treated in a ballast water tank. As a result, facility and operating costs can be reduced. Extinction of microbes of unlimited size and sterilization can be securely achieved without any strength drop on the side of treated liquid accommodation body. Further, the space for installation of detoxification apparatus for ballast water in ships can be reduced to thereby enable increasing of loading space for cargo, etc. Still further, on existing ships, the hull rework cost for installation of detoxification apparatus can be minimized.
    • 通过微生物去除将未处理液体转化为清洁的无害处理液体,其特征在于,对存在于液体中的微生物进行机械处理,从而实现其灭菌和灭菌,以及其中形成含氯物质的氯化物 将液体注入液体中,从而进行微生物灭绝和杀菌,将其施加于液体。 还提供了一种电解循环系统,包括对海水灭菌微生物进行解毒处理,并通过在陆地或海上的解毒设施对通过海水导入通道引入的海水进行灭菌,并将经过如此处理的海水容纳在 压载水箱。 因此,可以降低设施和运营成本。 在处理液体容纳体的一侧没有任何强度下降的情况下,可以可靠地实现无限尺寸和灭菌的微生物的灭绝。 此外,可以减少用于船舶中的压载水解毒装置的空间,从而能够增加货物的装载空间等。另外,在现有的船舶上,可以使安装解毒装置的船体返工成本最小化。
    • 6. 发明申请
    • Method of Liquid Detoxification and Apparatus Therefor
    • 液体排毒方法及其设备
    • US20080164217A1
    • 2008-07-10
    • US10569483
    • 2005-02-10
    • Kazuki NishizawaKazuhiko FujiseMasayuki TabataKiyoshi SugataRyouhei UedaTakashi UedaHirokazu OkadaTomoaki Oomura
    • Kazuki NishizawaKazuhiko FujiseMasayuki TabataKiyoshi SugataRyouhei UedaTakashi UedaHirokazu OkadaTomoaki Oomura
    • C02F9/00C02F9/06C02F1/76C02F1/38C02F1/46
    • B63J4/002B63B35/44C02F1/28C02F1/34C02F1/38C02F1/4674C02F2103/008Y02W10/37
    • Conversion of an untreated liquid to a clean harmless treated liquid through microbe removal, characterized in that a mechanical treatment for damaging microbes present in a liquid to thereby effect extinction thereof and sterilization, combined with a chlorination in which a chlorine-containing substance is formed from a liquid and injected into a liquid to thereby effect microbe extinction and sterilization, is applied to a liquid. There is further provided an electrolytic circulation system comprising applying a detoxification treatment for extinction of microbes in seawater and sterilization by means of detoxification facilities on land or on the sea to seawater introduced through a seawater introduction channel and accommodating the seawater having been thus treated in a ballast water tank. As a result, facility and operating costs can be reduced. Extinction of microbes of unlimited size and sterilization can be securely achieved without any strength drop on the side of treated liquid accommodation body. Further, the space for installation of detoxification apparatus for ballast water in ships can be reduced to thereby enable increasing of loading space for cargo, etc. Still further, on existing ships, the hull rework cost for installation of detoxification apparatus can be minimized.
    • 通过微生物去除将未处理液体转化为清洁的无害处理液体,其特征在于,对存在于液体中的微生物进行机械处理,从而实现其灭菌和灭菌,以及其中形成含氯物质的氯化物 将液体注入液体中,从而进行微生物灭绝和杀菌,将其施加于液体。 还提供了一种电解循环系统,包括对海水灭菌微生物进行解毒处理,并通过在陆地或海上的解毒设施对通过海水导入通道引入的海水进行灭菌,并将经过如此处理的海水容纳在 压载水箱。 因此,可以降低设施和运营成本。 在处理液体容纳体的一侧没有任何强度下降的情况下,可以可靠地实现无限尺寸和灭菌的微生物的灭绝。 此外,可以减少用于船舶中的压载水解毒装置的空间,从而能够增加货物的装载空间等。另外,在现有的船舶上,可以使安装解毒装置的船体返工成本最小化。