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1. 发明授权

US06465228B1 Levodione reductase 有权
标题翻译：左旋去氧还原酶
公开(公告)号：US06465228B1
公开(公告)日：2002-10-15
申请号：US09495523
申请日：2000-01-31
申请人： Shigeru Nakamori , Sakayu Shimizu , Masaru Wada
发明人： Shigeru Nakamori , Sakayu Shimizu , Masaru Wada
IPC分类号： C12N902
CPC分类号： C12N9/0008 , C12P7/26 , C12P41/002
摘要： A levodione reductase having the following physical properties is provided: molecular weight: from about 142,000 to about 155,000±10,000 (consisting of four homologous subunits having a molecular weight of 36,000±5,000); co-factor: nicotinamide adenine dinucleotide (AND/NADH); substrate specificity: active on levodione; optimum temperature: about 15° C. to about 20° C. at pH 7.0; optimum pH: 7.5; and activator: K+, Cs+, Rb+, Na+ and NH4+. The levodione reductase according to the present invention produces actinol, an important intermediate for the production of zeaxanthin, from levodione. This enzyme may be produced from a microorganism belonging to the genus Corynebacterium, preferably the microorganism Corynebacterium aquaticum AKU 611 (FERM BP-6448) or a functional equivalent, subculture, mutant or variant thereof. Also provided is a process for producing the levodione reductase that includes cultivating a microorganism of the genus Corynebacterium in an aqueous medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the levodione reductase from the cell-free extract. A process for producing actinol from levodione is also provided that includes contacting levodione with levodione reductase in the presence of the reduced form of nicotinamide adenine dinucleotide or a cell-free extract of the microorganism, and then isolating the resulting actinol from the reaction mixture.
摘要翻译：提供了具有以下物理性质的左旋二酮还原酶：分子量：约142,000至约155,000±10,000（由分子量为36,000±5,000的四个同源亚基组成）; 辅因子：烟酰胺腺嘌呤二核苷酸（AND / NADH）; 底物特异性：对左旋多巴有活性; 最适温度：约15℃至约20℃，pH 7.0; 最适pH：7.5; 和活化剂：K +，Cs +，Rb +，Na +和NH4 +。根据本发明的左氧多糖还原酶从左旋二酮生产作为玉米黄质生产的重要中间体的肌动蛋白。该酶可以由属于棒状杆菌属的微生物，优选微生物棒状杆菌属水杨酸AKU 611（FERM BP-6448）或其功能等同的传代培养，突变体或变体产生。还提供了生产左旋体还原酶的方法，其包括在需氧条件下在水性介质中培养棒状杆菌属的微生物，破坏微生物的细胞并从无细胞提取物中分离和纯化左旋体还原酶。还提供了用于从左旋二酮生产锕系元素的方法，包括在还原形式的烟酰胺腺嘌呤二核苷酸或微生物的无细胞提取物的存在下将左旋多糖与左旋二酮还原酶接触，然后从反应混合物中分离得到的锕系元素。

2. 发明申请

US20100093045A1 L-CYSTEINE PRODUCING MICROORGANISM AND A METHOD FOR PRODUCING L-CYSTEINE 有权
标题翻译：生产微生物的L-儿茶素和生产L- CYSTEINE的方法
公开(公告)号：US20100093045A1
公开(公告)日：2010-04-15
申请号：US12635404
申请日：2009-12-10
申请人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
发明人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
IPC分类号： C12P13/12
CPC分类号： C12P13/12
摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.
摘要翻译：通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

3. 发明申请

US20080076163A1 L-cysteine-producing microorganism and a method for producing L-cysteine 审中-公开
标题翻译： L-半胱氨酸生产微生物和L-半胱氨酸的生产方法
公开(公告)号：US20080076163A1
公开(公告)日：2008-03-27
申请号：US11070084
申请日：2005-03-03
申请人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
发明人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
IPC分类号： C12P13/12 , C12N1/21
CPC分类号： C12P13/12
摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.
摘要翻译：通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

4. 发明授权

US08114649B2 L-cysteine producing microorganism and a method for producing L-cysteine 有权
标题翻译： L-半胱氨酸生产微生物和L-半胱氨酸的生产方法
公开(公告)号：US08114649B2
公开(公告)日：2012-02-14
申请号：US12635404
申请日：2009-12-10
申请人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
发明人： Hiroshi Takagi , Shigeru Nakamori , Masaru Wada , Hirotada Mori
IPC分类号： C12N9/00 , C12N9/10 , C12P13/12 , C12N1/20
CPC分类号： C12P13/12
摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.
摘要翻译：通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，所述基因被修饰以使得半胱氨酸脱硫酸酶活性被降低或消除。将细菌培养在培养基中以产生并引起培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

5. 发明授权

US07148047B2 L-cysteine producing bacterium and method for producing L-cysteine 失效
公开(公告)号：US07148047B2
公开(公告)日：2006-12-12
申请号：US10060218
申请日：2002-02-01
申请人： Hiroshi Takagi , Masaru Wada , Shigeru Nakamori
发明人： Hiroshi Takagi , Masaru Wada , Shigeru Nakamori
IPC分类号： C12N9/10 , C12N1/12 , C12P13/12 , C07K1/00 , C07H21/02
CPC分类号： C12P13/12
摘要： L-cysteine is produced by culturing a coryneform bacterium in which intracellular serine acetyltransferase activity is increased by transformation with a gene coding for serine acetyltransferase, such modification of an expression control sequence of a gene coding for serine acetyltransferase that expression of the gene in a cell should be enhanced and for forth, preferably in which L-cysteine decomposition system is further suppressed, in a medium to produce and accumulate L-cysteine in culture and collecting the L-cysteine from the culture.

6. 发明授权

US06946268B2 L-cysteine producing bacterium and method for producing L-cysteine 有权
标题翻译： L-半胱氨酸生产细菌和L-半胱氨酸的制备方法
公开(公告)号：US06946268B2
公开(公告)日：2005-09-20
申请号：US10254763
申请日：2002-09-26
申请人： Hiroshi Takagi , Masaru Wada , Shigeru Nakamori
发明人： Hiroshi Takagi , Masaru Wada , Shigeru Nakamori
IPC分类号： C12N1/21 , C12P13/12 , C12R1/19 , C07H21/04 , C12N1/20 , C12N9/10 , C12N15/00
CPC分类号： C12R1/19 , C12N9/88 , C12P13/12
摘要： L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-β-lyase activity or cystathionine-β-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.
摘要翻译：通过培养具有L-半胱氨酸生产能力的埃希氏杆菌属细菌产生L-半胱氨酸，并进行修饰，以使得在培养基中减少或消除胱硫醚-β-裂解酶活性和色氨酸酶活性在培养基中产生并积累L-半胱氨酸，并从培养基中收集L-半胱氨酸。

7. 发明申请

US20050196861A1 Physiologically active peptides 失效
标题翻译：生理活性肽
公开(公告)号：US20050196861A1
公开(公告)日：2005-09-08
申请号：US11010800
申请日：2004-12-13
申请人： Shigeru Nakamori , Hiroshi Takagi , Masakazu Takahashi , Kazuhisa Tsujimoto , Hideyuki Yamada
发明人： Shigeru Nakamori , Hiroshi Takagi , Masakazu Takahashi , Kazuhisa Tsujimoto , Hideyuki Yamada
IPC分类号： C07K7/06 , C07K14/435 , C12N5/00 , C12N5/07 , A61K38/18 , C07K14/475 , C12N5/02
CPC分类号： C07K14/43586 , C12N5/0018 , C12N5/0601 , C12N2501/998 , C12N2501/999
摘要： A peptide according to the present invention is selected from the group consisting of: (a) a peptide having the amino acid sequence represented by SEQ ID NO: 1; (b) a peptide having a modified amino acid sequence of the amino acid sequence described in (a) above, in which one or more amino acid residues are deleted, substituted, inserted or added, and having cell death-preventing activity and/or cell growth-promoting activity; and (c) a peptide which has an amino acid sequence having at least 80% homology with the peptide consisting of the amino acid sequence described in (a) above and has cell death-preventing activity and/or cell growth-promoting activity. The peptide has cell death-preventing activity and/or cell growth-promoting activity, is highly safe, and can be produced without difficulty. The peptide is useful as a culture supplement or as an effective component for a cell culture medium.
摘要翻译：根据本发明的肽选自：（a）具有由SEQ ID NO：1表示的氨基酸序列的肽; （b）具有上述（a）中所述的氨基酸序列的修饰氨基酸序列的肽，其中一个或多个氨基酸残基被缺失，取代，插入或添加并具有细胞死亡预防活性和/或细胞生长促进活性; 和（c）具有与由上述（a）中所述的氨基酸序列组成的肽具有至少80％同源性的氨基酸序列并具有细胞死亡预防活性和/或细胞生长促进活性的肽。肽具有细胞死亡预防活性和/或细胞生长促进活性，是高度安全的，可以毫无困难地制备。该肽可用作培养物补充物或作为细胞培养基的有效成分。

8. 发明授权

US4885245A Process for producing L-tryptophan 失效
标题翻译：生产L-色氨酸的方法
公开(公告)号：US4885245A
公开(公告)日：1989-12-05
申请号：US760930
申请日：1985-07-31
申请人： Masaaki Ishida , Kiyoshi Miwa , Shigeru Nakamori , Konosuke Sano
发明人： Masaaki Ishida , Kiyoshi Miwa , Shigeru Nakamori , Konosuke Sano
IPC分类号： C12N15/09 , C12N1/21 , C12N9/88 , C12N15/00 , C12N15/77 , C12P13/22 , C12R1/13 , C12R1/15
CPC分类号： C12P13/227 , C12N15/77 , C12N9/88
摘要： A process for producing L-tryptophan in which a suitable substrate such as a carbohydrate, indole or anthranilic acid is contacted with Coryneform bacteria, where the Coryneform bacteria bear recombinant DNA constructed by connecting a gene coding for tryptophan synthetase with a plasmid vector capable of proliferating in Coryneform bacteria.
摘要翻译：一种生产L-色氨酸的方法，其中适当的底物如碳水化合物，吲哚或邻氨基苯甲酸与棒状细菌接触，其中棒状细菌携带通过将编码色氨酸合成酶的基因与能够增殖的质粒载体连接而构建的重组DNA 在棒状细菌。

9. 发明授权

US4778762A Plasmid 失效
标题翻译：质粒
公开(公告)号：US4778762A
公开(公告)日：1988-10-18
申请号：US434853
申请日：1982-10-18
申请人： Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
发明人： Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
IPC分类号： C12P13/08 , C12P13/14 , C12N15/00 , C12N1/20 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/13 , C12R1/15
CPC分类号： C12R1/13 , C12P13/08 , C12P13/14 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要： A substantially pure plasmid which is characterized by a molecular weight of 3.0.+-.0.1 megadalton and the restriction endonuclease-cleavage map shown in the Figure. This plasmid is capable of propagating in Corynebacteria and due to its size is useful as a vector for the cloning of exogenous genes.
摘要翻译：一种基本上纯的质粒，其特征在于分子量为3.0 +/- 0.1兆道尔顿和限制性内切核酸酶切割图，如图所示。该质粒能够在棒状杆菌中繁殖，并且由于其大小可用作克隆外源基因的载体。

10. 发明授权

US4757009A Recombinant DNA having a phosphoenol pyruvate carboxylase gene inserted therein, bacteria carrying said recombinant DNA and a process for producing amino acids using said bacteria 失效
标题翻译：插入有磷酸烯醇丙酮酸羧化酶基因的重组DNA，携带所述重组DNA的细菌和使用所述细菌产生氨基酸的方法
公开(公告)号：US4757009A
公开(公告)日：1988-07-12
申请号：US645107
申请日：1984-08-28
申请人： Konosuke Sano , Koichi Ito , Kiyoshi Miwa , Shigeru Nakamori
发明人： Konosuke Sano , Koichi Ito , Kiyoshi Miwa , Shigeru Nakamori
IPC分类号： C12N15/09 , C12N1/20 , C12N9/88 , C12N15/00 , C12N15/77 , C12P13/04 , C12P13/08 , C12P13/14 , C12P13/24 , C12R1/13 , C12R1/15 , C12N1/00 , C12P13/20
CPC分类号： C12P13/04 , C12N15/77 , C12N9/88 , C12P13/08 , C12P13/14 , C12P13/24 , Y10S435/84 , Y10S435/843
摘要： A recombinant DNA molecule comprising a plasmid vector having operationally inserted therein a gene coding for phosphoenol pyruvate carboxylase is disclosed along with bacteria containing this recombinant DNA molecule and methods of using these bacteria to produce amino acids in large quantities.
摘要翻译：公开了包含可操作地插入其中编码磷酸烯醇丙酮酸羧化酶的基因的质粒载体的重组DNA分子以及含有该重组DNA分子的细菌以及使用这些细菌大量生成氨基酸的方法。

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