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    • 1. 发明授权
    • Method and reagent for determination of dehydrogenase or its substrate
    • 用于测定脱氢酶或其底物的方法和试剂
    • US5250420A
    • 1993-10-05
    • US746202
    • 1991-08-15
    • Shigeki AsanoHaruo WatanabeYuzo Hayashi
    • Shigeki AsanoHaruo WatanabeYuzo Hayashi
    • C12Q1/28C12Q1/32
    • C12Q1/32C12Q1/28Y10S435/81Y10S436/904
    • A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to dehydrogenase reaction in the presence of an electron carrier or a combination of a coenzyme and an electron carrier, allowing to form H.sub.2 O.sub.2 through transfer of electrons derived from the substrate by the dehydrogenase reaction to O.sub.2 via the electron carrier or via the coenzyme and electron carrier, stopping the dehydrogenase reaction, reacting said H.sub.2 O.sub.2 with a peroxidase and a chromogen, and measuring a formed dye, and a reagent for determining dehydrogenase or its substrate. By the method and the reagent, dehydrogenase or its substrate can be accurately determined in a short time regardless of the concentration of the dehydrogenase and/or the substrate and without staining tubes or cells of a determination device with a dye.
    • 一种用于在脱氢酶反应中测定脱氢酶或其底物的方法,其包括在电子载体或辅酶和电子载体的组合的存在下使脱氢酶及其底物进行脱氢酶反应,通过转移电子形成H 2 O 2 通过脱氢酶反应从底物通过电子载体或通过辅酶和电子载体脱氧酶,停止脱氢酶反应,使所述H 2 O 2与过氧化物酶和色原反应,并测量形成的染料,以及用于测定脱氢酶或其衍生物的试剂 基质。 通过该方法和试剂,可以在短时间内精确测定脱氢酶或其底物,而不管脱氢酶和/或底物的浓度如何,而不用用染料污染测定装置的管或细胞。