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1. 发明申请

US20120196294A1 WORKFLOW FOR DETECTION OF LIGANDS USING NUCLEIC ACIDS 审中-公开
标题翻译：检测使用核酸的配体的工作流程
公开(公告)号：US20120196294A1
公开(公告)日：2012-08-02
申请号：US13352237
申请日：2012-01-17
申请人： Shiaw-Min Chen , Elana Swartzman , David Ruff , Mark Shannon , Julia Lu , Stephen Hendricks
发明人： Shiaw-Min Chen , Elana Swartzman , David Ruff , Mark Shannon , Julia Lu , Stephen Hendricks
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6804 , C12Q2537/101 , C12Q2561/101 , C12Q2561/125
摘要： This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.
摘要翻译：本申请涉及用于连接与靶核酸互补的寡核苷酸的方法，并扩增连接的寡核苷酸，其中连接和扩增发生在相同的反应混合物中。

2. 发明授权

US08932813B2 Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions 有权
标题翻译：通过多磷酸裂解（APP）反应的活化聚合核酸
公开(公告)号：US08932813B2
公开(公告)日：2015-01-13
申请号：US13324676
申请日：2011-12-13
申请人： Lei (Larry) Xi , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
发明人： Lei (Larry) Xi , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
摘要： This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
摘要翻译：本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。

3. 发明授权

US08703461B2 Mutant RB69 DNA polymerase 有权
标题翻译：突变型RB69 DNA聚合酶
公开(公告)号：US08703461B2
公开(公告)日：2014-04-22
申请号：US13600416
申请日：2012-08-31
申请人： Marian Peris , Michael Phelan , Barnett Rosenblum , Stephen Hendricks
发明人： Marian Peris , Michael Phelan , Barnett Rosenblum , Stephen Hendricks
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
摘要翻译：本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。这些突变型聚合酶能够选择性地结合标记的核苷酸。这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

4. 发明申请

US20050149033A1 Bioabsorbable fasteners for preparing and securing ligament replacement grafts 审中-公开
标题翻译：用于制备和固定韧带置换移植物的生物可吸收紧固件
公开(公告)号：US20050149033A1
公开(公告)日：2005-07-07
申请号：US10971721
申请日：2004-10-22
申请人： David McGuire , Stephen Hendricks
发明人： David McGuire , Stephen Hendricks
IPC分类号： A61B17/16 , A61B17/17 , A61F2/08 , A61B17/56
CPC分类号： A61F2/0811 , A61B17/1635 , A61B17/1714 , A61B17/1764 , A61F2002/0829 , A61F2002/087 , A61F2002/0882 , A61F2210/0004 , A61F2240/005
摘要： A bioabsorbable implantable device for replacing sutures in construction of a composite graft in ligament replacement surgery. In certain embodiments the device has a female component and a male component where the female component receives and secures the male component. In other embodiments the components of the device are in the shape of a rivet or a staple. The bioabsorbable implantable devices can be used for securing tendon grafts to bone blocks and for holding together the fibers of the tendon graft when the bone-tendon-bone graft is inserted into a patient during surgery. The bioabsorbable implantable device may also be part of a package for use in surgery. The package includes a sterile container for holding at least a graft that is of a predetermined length and width. The package may also include bone blocks. The package is marked as to the graft size including the width and the length of the graft. The graft and the bone blocks may be autogenous, allogenic or constructed from man-made materials.
摘要翻译：一种生物可吸收的可植入装置，用于在韧带置换手术中替代复合移植物的结构中的缝合线。在某些实施例中，该装置具有阴部件和阳部件，其中阴部件接收并固定阳部件。在其它实施例中，装置的部件是铆钉或钉的形状。生物可吸收的可植入装置可以用于将腱移植物固定到骨块上，并且当在手术期间将骨腱 - 骨移植物插入患者时，将腱移植物的纤维保持在一起。生物可吸收可植入装置也可以是用于手术的包装的一部分。该包装包括用于至少保持具有预定长度和宽度的移植物的无菌容器。包装还可以包括骨块。包装被标记为移植物尺寸，包括移植物的宽度和长度。移植物和骨块可以是自生的，同种异体的或由人造材料构成的。

5. 发明申请

US20120196329A1 POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS 有权
标题翻译：使用多磷酸（APP）反应活化的核酸的聚合
公开(公告)号：US20120196329A1
公开(公告)日：2012-08-02
申请号：US13324676
申请日：2011-12-13
申请人： Lei (Larry) XI , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
发明人： Lei (Larry) XI , Paul Kenney , Zhaochun Ma , Dennis Prosen , Stephen Hendricks , Roland Nagel
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
摘要： This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
摘要翻译：本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。

6. 发明申请

US20110159551A1 cDNA SYNTHESIS USING A REVERSIBLY INACTIVATED REVERSE TRANSCRIPTASE 审中-公开
标题翻译：使用反义寡核苷酸逆转录酶进行cDNA合成
公开(公告)号：US20110159551A1
公开(公告)日：2011-06-30
申请号：US12900794
申请日：2010-10-08
申请人： Lei (a.k.a Larry) Xi , Roland Nagel , Stephen Hendricks , Jennifer Berkman , Marian Peris , Yulei Wang
发明人： Lei (a.k.a Larry) Xi , Roland Nagel , Stephen Hendricks , Jennifer Berkman , Marian Peris , Yulei Wang
IPC分类号： C12P19/34 , C12N9/10
CPC分类号： C12N9/1276 , C12N15/1096 , C12Q2521/107 , C12Y207/07049
摘要： The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a signficant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.
摘要翻译：本发明提供使用可逆失活的逆转录酶进行逆转录反应的组合物和方法。可逆灭活的逆转录酶由化学修饰产生，其使逆转录酶失活。通过在反转录反应之前或作为逆转录反应的一部分的升高温度下温育反应物来回收逆转录酶的活性。本发明的逆转录酶提供了明显减少模板核酸分子的非特异性逆转录，因为反应混合物的配方不支持在逆转录酶激活之前形成逆转录产物。

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