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1. 发明授权

US07557266B2 Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize D9 gene and methods of use 失效
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子及其使用方法
公开(公告)号：US07557266B2
公开(公告)日：2009-07-07
申请号：US11736615
申请日：2007-04-18
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： A01H5/00 , C12N5/14 , C12N15/63 , C07H21/04
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。

2. 发明申请

US20120005786A1 Isolated Polynucleotide Molecules Corresponding to Mutant and Wild-Type Alleles of the Maize D9 Gene and Methods of Use 审中-公开
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子和使用方法
公开(公告)号：US20120005786A1
公开(公告)日：2012-01-05
申请号：US13206081
申请日：2011-08-09
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： A01H1/00 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides and transformed plants, seeds and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。

3. 发明申请

US20090172841A1 Isolated Polynucleotide Molecules Corresponding to Mutant and Wild-Type Alleles of the Maize D9 Gene and Methods of Use 审中-公开
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子和使用方法
公开(公告)号：US20090172841A1
公开(公告)日：2009-07-02
申请号：US12392169
申请日：2009-02-25
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： C12N15/82 , C07K14/00
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了修饰涉及使用这些分离的多核苷酸分子，分离的多肽以及转化的植物，种子和细胞的植物生长的方法。

4. 发明申请

US20110023190A1 USE OF DIMERIZATION DOMAIN COMPONENT STACKS TO MODULATE PLANT ARCHITECTURE 审中-公开
标题翻译：使用二维域组件堆栈来调节植物建筑
公开(公告)号：US20110023190A1
公开(公告)日：2011-01-27
申请号：US12837553
申请日：2010-07-16
申请人： Shai J. Lawit , Dwight T. Tomes
发明人： Shai J. Lawit , Dwight T. Tomes
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8261 , C07C31/08 , C07K14/415 , C12N15/8242 , C12N15/8255 , C12N15/8269 , C12N15/827 , C12N15/8293 , C12N15/8297 , C12P7/06 , Y02A40/146
摘要： This invention provides means for altering the harvest index of crop plants by modulating the expression of transgenic genes using dimerization domain and component stacks, thereby modulating plant architecture. The transgene/dimerization domain stacks are provided in a single transformation vector unit and are used to modulate plant growth, yield, and harvest index in plants.
摘要翻译：本发明提供了通过使用二聚化域和组分堆栈调节转基因基因的表达来改变作物植物收获指数的方法，从而调节植物结构。提供转基因/二聚化结构域的单个转化载体单位，并用于调节植物的植物生长，产量和收获指数。

5. 发明申请

US20100269229A1 Isolated Polynucleotide Molecules Corresponding to Mutant and Wild-Type Alleles of the Maize D9 Gene and Methods of Use 审中-公开
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子和使用方法
公开(公告)号：US20100269229A1
公开(公告)日：2010-10-21
申请号：US12823240
申请日：2010-06-25
申请人： Shai J. Lawit , Suman Kundu , Aragula G Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G Rao , Dwight T. Tomes
IPC分类号： C12N15/82 , C12N5/10 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。

6. 发明申请

US20130180006A1 Pollen Preferred Promoters and Methods of Use 有权
标题翻译：花粉优选的启动子和使用方法
公开(公告)号：US20130180006A1
公开(公告)日：2013-07-11
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , C12N5/10
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

7. 发明申请

US20130180010A1 Methods and Compositions for Modulating Expression or Activity of a RKD Polypeptide in a Plant 审中-公开
公开(公告)号：US20130180010A1
公开(公告)日：2013-07-11
申请号：US13445447
申请日：2012-04-12
申请人： Mark A. Chamberlin , Shai J. Lawit
发明人： Mark A. Chamberlin , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , A01H5/00 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8261 , C12N15/8287
摘要： Methods and compositions are provided to increase the activity/level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity/level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.

8. 发明申请

US20130180007A1 Somatic Ovule Specific Promoters and Methods of Use 审中-公开
标题翻译：体细胞特异性启动子和使用方法
公开(公告)号：US20130180007A1
公开(公告)日：2013-07-11
申请号：US13445391
申请日：2012-04-12
申请人： Mark A. Chamberlin , Shai J. Lawit
发明人： Mark A. Chamberlin , Shai J. Lawit
IPC分类号： C12N15/82
CPC分类号： C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12N15/8278 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8283 , C12N15/8286 , C12N15/8287 , C12N15/8289 , Y02A40/146
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for several Arabidopsis thaliana ovule somatic tissue-preferred promoters AT-CYP86C1, AT-PPM, AT-EXT, AT-GILT1 and AT-TT2. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括几个拟南芥胚珠体细胞组织优选启动子AT-CYP86C1，AT-PPM，AT-EXT，AT-GILT1和AT-TT2的核苷酸序列。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

9. 发明申请

US20130180005A1 Method to Screen Plants for Genetic Elements Inducing Parthenogenesis in Plants 审中-公开
标题翻译：筛选植物遗传元素诱导植物中孤雌生殖的方法
公开(公告)号：US20130180005A1
公开(公告)日：2013-07-11
申请号：US13445276
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： A01H1/06 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8287 , C12N15/8233
摘要： Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.
摘要翻译：提供了缺乏性衍生胚胎的植物群体的组合物和方法。组合物包括编码多核苷酸的抑制盒和产生单性生成的启动子。进一步提供的是孤雌生殖遗传元件，用于防止自我繁殖植物中的有性繁殖。方法包括：利用作为无菌近交维持系统保持的母体胚胎缺陷隐性突变，允许产生对于隐性突变体等位基因是纯合的群体，但是经过补充。方法包括利用通过卵细胞特异性启动子表达的毒素基因，产生显性的无胚胎表型，通过雌性配子不可透过。用驱动解毒剂的卵细胞启动子，花粉消融PTU和用于鉴定转基因种子的种子颜色标记物转化所产生的半合子植物。一代植物50％的女性可育，有种子，当下一代种植时，将产生具有50％活力的转基因种子和50％不能存活的无胚芽种子的植物。

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