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1. 发明授权

US08183017B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US08183017B2
公开(公告)日：2012-05-22
申请号：US12915793
申请日：2010-10-29
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.
摘要翻译：当棒状细菌含有其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏的天冬氨酸激酶时，棒状细菌可以产生L-赖氨酸的能力和速度得到改善。这是通过连续增强编码二氢吡啶二羧酸还原酶的DNA，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA来实现的。

2. 发明授权

US07846698B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US07846698B2
公开(公告)日：2010-12-07
申请号：US10226136
申请日：2002-08-23
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.
摘要翻译：通过连续增强编码二氢吡啶二羧酸还原酶的DNA，在含有赖氨酸和L-苏氨酸的反馈抑制实质上脱敏的天麸蛋白的棒状细菌的L-赖氨酸生产能力和L-赖氨酸生产速度得到改善，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA，以及编码二氨基庚二酸脱氢酶的DNA。

3. 发明授权

US6090597A Method of producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US6090597A
公开(公告)日：2000-07-18
申请号：US852730
申请日：1997-05-07
申请人： Seiko Hirano , Masakazu Sugimoto , Eiichi Nakano , Masako Izui , Atsushi Hayakawa , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Hirano , Masakazu Sugimoto , Eiichi Nakano , Masako Izui , Atsushi Hayakawa , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/06 , C12N9/88 , C12N15/77 , C12P13/08 , C12R1/13 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0016 , C12N15/77 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.
摘要翻译：在培养基中培养其中编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA的棒状细菌在培养基中培养以使L-赖氨酸在培养物中产生并积累，从培养物中收集L-赖氨酸。

4. 发明授权

US06221636B1 Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US06221636B1
公开(公告)日：2001-04-24
申请号：US08985916
申请日：1997-12-05
申请人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P1308
CPC分类号： C12N9/1217 , C12N9/88 , C12P13/08
摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.
摘要翻译：在棒状杆菌细胞的细胞中自主复制的重组DNA，其中包含编码天冬氨酸激酶的DNA序列，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法，其特征在于，在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，从培养物中收集L-赖氨酸。

5. 发明授权

US06893852B1 Dna encoding sucrose pts enzyme II 失效
标题翻译： Dna编码蔗糖酶Ⅱ
公开(公告)号：US06893852B1
公开(公告)日：2005-05-17
申请号：US10019284
申请日：2000-06-30
申请人： Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Masako Izui , Masakazu Sugimoto , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N9/12 , C12N15/54 , C07H21/04
CPC分类号： C12N9/1205 , C12Y207/01069
摘要： A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.
摘要翻译：通过盒式连接介导的PCR扩增棒状杆菌型细菌的蔗糖酶基因下游的区域，得到编码构成棒状细菌的蔗糖PTS的蛋白质的基因，得到编码下述定义的蛋白质的蔗糖PTS酶II的DNA（ A）或（B）：（A）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质; （B）序列表中具有SEQ ID NO：2的氨基酸序列的蛋白质，包括一个或几个氨基酸的取代，缺失，插入，添加或转化，以及与蔗糖结合的活性。

6. 发明授权

US5804414A Method of amplifying genes using artificial transposons in coryneform bacteria 失效
标题翻译：在棒状细菌中使用人工转座子扩增基因的方法
公开(公告)号：US5804414A
公开(公告)日：1998-09-08
申请号：US674168
申请日：1996-07-01
申请人： Mika Moriya , Hiroshi Matsui , Kenzo Yokozeki , Seiko Hirano , Atsushi Hayakawa , Masako Izui , Masakazu Sugimoto
发明人： Mika Moriya , Hiroshi Matsui , Kenzo Yokozeki , Seiko Hirano , Atsushi Hayakawa , Masako Izui , Masakazu Sugimoto
IPC分类号： C12N1/21 , C12N9/02 , C12N9/12 , C12N9/88 , C12N15/67 , C12N15/77 , C12N15/87 , C12N15/90 , C12P13/04 , C12P13/08 , C12P21/00 , C12P21/04
CPC分类号： C12N9/001 , C12N15/67 , C12N15/77 , C12N15/90 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted into an insertion sequence of the coryneform bacterium, and introducing said artificial transposon into the coryneform bacterium. In accordance with the method of the present invention, a desired gene can be amplified in a chromosome in coryneform bacteria which are used in the industrial production of amino acids or nucleic acids.
摘要翻译：一种扩增棒状细菌染色体中所需基因的方法，其特征在于包括形成其中将耐药基因和所需基因插入棒状细菌的插入序列的人工转座子，并将所述人工转座子引入棒状细胞细菌。根据本发明的方法，可以在用于工业生产氨基酸或核酸的棒状细菌的染色体中扩增所需的基因。

7. 发明授权

US6004773A Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US6004773A
公开(公告)日：1999-12-21
申请号：US985908
申请日：1997-12-05
申请人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/53 , C12N15/54 , C12N15/60 , C12N15/77 , C12P13/08 , C12P1/00 , C07H21/04 , C12N15/63 , C12P21/02
CPC分类号： C12N9/001 , C12N15/77 , C12N9/1096 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.
摘要翻译：含有赖氨酸和L-苏氨酸的反馈抑制基本脱敏的天冬氨酸激酶的棒状细菌，包含编码二氢吡啶二羧酸还原酶的增强型DNA序列，编码二氢吡啶啉酮还原酶的增强型DNA序列，编码二氢吡啶酸盐的增强型DNA序列合成酶，编码二氨基庚二酸脱羧酶的增强的DNA序列和编码天冬氨酸转氨酶的增强的DNA序列; 一种生产L-赖氨酸的方法，包括以下步骤：在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，并从培养物中收集L-赖氨酸; 以及可用于生产棒状细菌的重组DNA。

8. 发明授权

US5616480A Temperature sensitive plasmid 失效
标题翻译：温度敏感质粒
公开(公告)号：US5616480A
公开(公告)日：1997-04-01
申请号：US408188
申请日：1995-03-22
申请人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
发明人： Masakazu Sugimoto , Hiroyuki Kojima , Akiko Tanaka , Hiroshi Matsui , Katsuaki Sato , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C12N1/21 , C12N9/00 , C12N9/12 , C12N15/63 , C12N15/77 , C12P13/08 , C12P13/22 , C12R1/13 , C12N1/20
CPC分类号： C12N9/00 , C12N15/77 , C12N9/12 , C12P13/08 , C12P13/22
摘要： A plasmid having a temperature-sensitive replication origin which has the following properties:a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and,b) when a cell containing said plasmid is cultured at 31.degree. to 37.degree. C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid.
摘要翻译：具有温度敏感复制起点的质粒，其具有以下性质：a）能够在棒状杆菌中自主复制并保留在棒状杆菌中; 和b）当含有所述质粒的细胞在31℃至37℃下培养时，质粒的复制被抑制，同时从细胞体中除去质粒; 以及使用该质粒在棒状杆菌中进行同源重组的方法。

9. 发明授权

US06258573B1 Method of producing L-serine by fermentation 失效
标题翻译：通过发酵生产L-丝氨酸的方法
公开(公告)号：US06258573B1
公开(公告)日：2001-07-10
申请号：US09222786
申请日：1998-12-30
申请人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
发明人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
IPC分类号： C12P1306
CPC分类号： C12N9/0006 , C12P13/06 , C12R1/13 , C12R1/15 , C12R1/19
摘要： Disclosed is a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.
摘要翻译：公开了具有对丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有L-丝氨酸生产力的抗性的棒状细菌。此外，公开了衍生自棒状细菌的D-3-磷酸甘油酸脱氢酶，其中L-丝氨酸的反馈抑制被脱敏; D-3-磷酸甘油酸脱氢酶，其可从具有对β-丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有抗丝氨酸生产力的棒状细菌获得; 具有序列表中SEQ ID NO：12所示氨基酸序列的D-3-磷酸甘油酸脱氢酶或包含一个或多个氨基酸的取代，添加或缺失的序列，其中对应于第325位谷氨酸残基的氨基酸残基 SEQ ID NO：12中的氨基酸序列被除了谷氨酸以外的氨基酸替换; 具有序列表中SEQ ID NO：11所示的氨基酸序列的D-3-磷酸甘油酸脱氢酶; 编码上述D-3-磷酸甘油酸脱氢酶的DNA; 具有序列表中SEQ ID NO：13所示碱基序列的DNA; 含有含DNA的重组DNA的棒状细菌; 以及生产L-丝氨酸的方法，包括以下步骤：在培养基中培养上述细菌以允许L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

10. 发明授权

US6037154A Method of producing L-serine by fermentation 有权
标题翻译：通过发酵生产L-丝氨酸的方法
公开(公告)号：US6037154A
公开(公告)日：2000-03-14
申请号：US222817
申请日：1998-12-30
申请人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
发明人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/04 , C12N15/53 , C12P13/06 , C12R1/13 , C12N1/12 , C12N9/14
CPC分类号： C12N9/0006 , C12P13/06 , C12R1/13 , C12R1/15 , C12R1/19
摘要： L-serine is produced by cultivating in a medium a coryneform bacterium having L-serine productivity in which an activity of at least one of phosphoserine phosphatase and phosphoserine transaminase is enhanced, preferably, further having introduced therein a gene coding for D-3-phosophoglycerate dehydrogenase in which feedback inhibition by L-serine is desensitized, allowing L-serine to accumulate in the medium, and collecting the L-serine from the medium.
摘要翻译：通过在培养基中培养具有L-丝氨酸生产力的棒状细菌，其中磷酸丝氨酸磷酸酶和磷酸丝氨酸转氨酶中的至少一种的活性增强，优选进一步在其中引入编码D-3-磷酸甘油酸的基因而产生L-丝氨酸其中L-丝氨酸的反馈抑制脱敏，使L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

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