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1. 发明申请

US20100152419A1 FUSION PROTEIN CONTAINING HIGHLY-EXPRESSED AND SECRETED INSULIN PRECURSOR, DNA ENCODING SAME, AND METHOD OF PRODUCING INSULIN 审中-公开
标题翻译：含有高表达和分泌胰岛素前体的融合蛋白，编码其的DNA及其生产胰岛素的方法
公开(公告)号：US20100152419A1
公开(公告)日：2010-06-17
申请号：US12162473
申请日：2008-02-12
申请人： Seiji Sato , Masa-aki Kondo , Toshiyuki Kudo , Ko-suke Endo
发明人： Seiji Sato , Masa-aki Kondo , Toshiyuki Kudo , Ko-suke Endo
IPC分类号： C07K14/62 , C07H21/04 , C12N15/63 , C12N1/21 , C12P21/02
CPC分类号： C07K14/62 , C07K2319/01
摘要： A DNA which encodes a fused protein containing an insulin precursor of the overexpression secretion type for producing transgenic insulin, and a method of producing insulin with the use of this DNA.
摘要翻译：编码含有用于产生转基因胰岛素的过表达分泌型胰岛素前体的融合蛋白的DNA，以及使用该DNA生产胰岛素的方法。

2. 发明授权

US5461145A Sexing method of bovine embyros 失效
标题翻译：牛的embyros的性别的方法
公开(公告)号：US5461145A
公开(公告)日：1995-10-24
申请号：US984044
申请日：1992-12-02
申请人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
发明人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6879
摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
摘要翻译：本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

3. 发明授权

US07037684B2 Process for producing polypeptide having disulfide bond 失效
标题翻译：制备具有二硫键的多肽的方法
公开(公告)号：US07037684B2
公开(公告)日：2006-05-02
申请号：US10221677
申请日：2001-03-13
申请人： Shigezo Udaka , Seiji Sato , Toshiyuki Kudo , Shusaku Oka , Naohiko Higashikuni , Masaaki Kondo
发明人： Shigezo Udaka , Seiji Sato , Toshiyuki Kudo , Shusaku Oka , Naohiko Higashikuni , Masaaki Kondo
IPC分类号： C07H21/04 , C12P21/04 , C12N1/20 , C12N15/00 , A61K38/28
CPC分类号： C12P21/02 , C07K14/32 , C07K14/62 , C07K2319/02 , C07K2319/035 , C07K2319/75 , C12N9/90 , C12N15/625 , C12N15/75
摘要： The invention relates to a DNA that enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds. The invention further relates to a process of producing polypeptides using the DNA and a process of enhancing the efficiency of formation of correct disulfide bond in an expression system of a gene recombinant polypeptide having disulfide bonds.
摘要翻译：本发明涉及能够共表达蛋白质二硫键异构酶和具有二硫键的多肽的DNA。本发明还涉及使用该DNA产生多肽的方法和在具有二硫键的基因重组多肽的表达系统中提高正确二硫键形成的效率的方法。

4. 发明授权

US06506595B2 DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs 失效
标题翻译：编码新融合蛋白的DNA和通过DNA表达制备有用多肽的方法
公开(公告)号：US06506595B2
公开(公告)日：2003-01-14
申请号：US09280030
申请日：1999-03-26
申请人： Seiji Sato , Naohiko Higashikuni , Toshiyuki Kudo , Masaaki Kondo
发明人： Seiji Sato , Naohiko Higashikuni , Toshiyuki Kudo , Masaaki Kondo
IPC分类号： C12N1500
CPC分类号： C12N15/625 , C07K14/605 , C07K14/61 , C07K14/62 , C07K14/655 , C07K2319/034 , C07K2319/21 , C07K2319/50 , C07K2319/75
摘要： This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of signal peptide for Bacillus cell wall protein (CWP); a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, the sequences being linked in order, the signal peptide, tag and linker being optional sequences; and wherein the nucleotide sequence encoding a fusion protein is ligated to 3′-end of a nucleic acid sequence comprising a Bacillus promoter region; to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium.
摘要翻译：本发明涉及包含编码融合蛋白的核苷酸序列的DNA，其中融合蛋白包含：用于芽孢杆菌细胞壁蛋白（CWP）的信号肽序列; 用于分离和纯化融合蛋白的标签序列; 接头序列; 用于化学或酶切割的序列; 和外源多肽序列，序列依次连接，信号肽，标签和接头是任选的序列; 并且其中将编码融合蛋白的核苷酸序列连接到包含芽孢杆菌启动子区的核酸序列的3'-末端; 涉及包含该DNA的载体; 涉及属于包含载体的芽孢杆菌属的细菌; 以及通过细菌培养制备有用多肽的方法。

5. 发明授权

US5661011A Sexing method of bovine embryos 失效
标题翻译：牛胚胎的性别方法
公开(公告)号：US5661011A
公开(公告)日：1997-08-26
申请号：US458393
申请日：1995-06-02
申请人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
发明人： Toshiyuki Kudo , Yoshiaki Itagaki , Seiji Sato , Shizuyo Sutou , Toyoo Nakamura
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6879
摘要： The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
摘要翻译：本发明提供可以容易地获得牛胚胎性别的PCR引物，并提供使用这些引物确定牛胚胎性别的实用，快速和可靠的方法。用于确定牛胚胎性别的方法的特征在于区分通过用雄性特异性和性别中性引物的PCR扩增特异性DNA序列获得的PCR产物。这些引物衍生自与牛雄性基因组特异性杂交的DNA，以及与牛雄性和雌性基因组性别中性杂交的DNA。

6. 发明授权

US06841361B1 Methods of producing recombinant insulin from novel fused proteins 失效
标题翻译：从新型融合蛋白生产重组胰岛素的方法
公开(公告)号：US06841361B1
公开(公告)日：2005-01-11
申请号：US09959548
申请日：2000-04-26
申请人： Shusaku Oka , Seiji Sato , Naohiko Higashikuni , Masaaki Kondo , Toshiyuki Kudo , Shigeaki Watanabe , Yoshihiro Waki , Hirotaka Yuki
发明人： Shusaku Oka , Seiji Sato , Naohiko Higashikuni , Masaaki Kondo , Toshiyuki Kudo , Shigeaki Watanabe , Yoshihiro Waki , Hirotaka Yuki
IPC分类号： C12N15/09 , C07K14/575 , C07K14/62 , C07K19/00 , C12N1/21 , C12N15/00 , C12N15/17 , C12P21/02 , C12R1/08 , C12P21/04
CPC分类号： C07K14/62 , C07K14/575 , C07K2319/00
摘要： This invention relates to a DNA encoding a fusion protein of formula (I) [Y]-[X1]-[B-chain]-[X2]-[Linker]-[X3]-[A-chain] (I) wherein Y is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue; X1 is an amino acid sequence which is cleavable enzymatically or chemically; B-chain is the amino acid sequence of insulin B chain; X2 is an amino acid sequence which is cleavable enzymatically; Linker is a linker sequence comprising at least one amino acid residue; X3 is an amino acid sequence which is cleavable enzymatically; and A- chain is the amino acid sequence of insulin A chain, and to a method for producing insulin using an expression system containing the DNA in high efficiency and high yield.
摘要翻译：本发明涉及编码式（I）的融合蛋白的DNA，其中Y是蛋白质的表达和分泌的前导肽序列，其包含至少一个氨基酸残基; X1是可酶切或化学切割的氨基酸序列 B链是胰岛素B链的氨基酸序列; X2是可酶切的氨基酸序列;接头是包含至少一个氨基酸残基的接头序列; X3是可酶切的氨基酸序列; A链是胰岛素A链的氨基酸序列，以及使用含有高效率和高收率的DNA的表达系统来生产胰岛素的方法。

7. 发明授权

US4450551A Keel-tipped stylus, and method and device for making keel-tipped stylus 失效
标题翻译：龙骨触针，以及制作龙骨触针的方法和装置
公开(公告)号：US4450551A
公开(公告)日：1984-05-22
申请号：US388634
申请日：1982-06-15
申请人： Toshiyuki Kudo , Kazuo Itoh , Masafumi Kitamura
发明人： Toshiyuki Kudo , Kazuo Itoh , Masafumi Kitamura
IPC分类号： G11B11/00 , B24B19/16 , G11B3/44 , G11B3/46 , G11B9/07 , G11B9/06 , G11B3/00
CPC分类号： G11B3/46 , B24B19/16 , G11B3/445 , G11B9/075
摘要： A keel-tipped stylus has a constricted terminal portion which is mounted on narrow shoulders which is in turn mounted on wide shoulders. The keel-tipped stylus is formed from a V-shaped stylus which engages into a spiral groove formed in an abrasive lapping disc. As the lapping disc rotates, the V-shaped stylus runs along the groove to lap the constricted terminal portion together with the narrow and wide shoulders.
摘要翻译：龙骨式触针具有收缩的端子部分，该端部部分安装在窄肩部上，肩部又安装在宽肩部上。龙骨笔的触针由V形触针形成，该V形触针接合到形成在磨料研磨盘中的螺旋槽中。当研磨盘旋转时，V形触针沿着凹槽延伸，以将狭窄的末端部分与窄而宽的肩部一起搭接。

8. 发明申请

US20080280542A1 CLEANING APPARATUS FOR A PROBE 审中-公开
标题翻译：清洁设备探针
公开(公告)号：US20080280542A1
公开(公告)日：2008-11-13
申请号：US12115495
申请日：2008-05-05
申请人： Yuji Miyagi , Tetsuya Iwabuchi , Toshiyuki Kudo
发明人： Yuji Miyagi , Tetsuya Iwabuchi , Toshiyuki Kudo
IPC分类号： B24B7/00
CPC分类号： B24B19/16 , B24B19/226
摘要： The present invention provides a cleaning apparatus capable of removing foreign matters attached to a tip of a probe effectively without impairing the durability of the probe. The cleaning apparatus for the probe comprises a base plate having a rough surface and a surface layer formed to conform to and cover the rough surface for the purpose of providing a polishing surface for the probe and having lower hardness than hardness of the probe tip of the probe.
摘要翻译：本发明提供一种清洁装置，其能够有效地除去附着于探针的尖端的异物，而不会损害探针的耐久性。用于探针的清洁装置包括具有粗糙表面的基板和形成为符合并覆盖粗糙表面的表面层，以提供用于探针的抛光表面，并且具有比硬度更高的探针尖端的硬度探测。

9. 发明授权

US06952686B1 Electronic contract safekeeping method, electronic contract verifying method, contractor server, contract safekeeping server, electronic contract safekeeping system, and memory medium 失效
标题翻译：电子合同保管方式，电子合同验证方法，承包商服务器，合同保管服务器，电子合同保管系统和存储介质
公开(公告)号：US06952686B1
公开(公告)日：2005-10-04
申请号：US09926148
申请日：2000-09-25
申请人： Toshiyuki Kudo
发明人： Toshiyuki Kudo
IPC分类号： G06Q30/00 , G21C17/60
CPC分类号： G06Q30/06 , G06Q20/401 , G06Q50/188
摘要： An electronic safekeeping method, including displaying a contractual coverage of a contract at a covenantee terminal connected to a contractor server storing information of the contractual coverage; inputting contract agreement information representing whether agreeing to the contractual coverage or not, at the covenantee terminal; inputting safekeeping agreement information representing whether agreeing to a safekeeping of the contractual coverage or not, at the covenantee terminal, when the contract agreement information agreeing to the contractual coverage is inputted; requesting a contract safekeeping server to store the contractual coverage, when the safekeeping agreement information agreeing to the safekeeping is inputted; issuing a contract ID for identifying the contract at the contract safekeeping server in accordance with the request; and transferring the issued contract ID from the contract safekeeping server to the covenantee terminal and the contractor server.
摘要翻译：一种电子保管方法，包括在与承包商服务器连接的公约终端上显示合同的合同范围，存储合同范围的信息; 在契约方终端输入代表是否同意合同范围的合同协议信息; 在合同协议信息同意输入合同协议信息时，在契约终端输入代表是否同意保管合同范围的保管协议信息; 当输入同意保管的保管协议信息时，请求合同保管服务器存储合同范围; 根据要求，在合同保管服务器上签发合同识别合同; 并将已签发的合同编号从合同保管服务器转移给契约终端和承包商服务器。

10. 发明授权

US08680880B2 Method and apparatus for testing integrated circuit 有权
标题翻译：集成电路测试方法和装置
公开(公告)号：US08680880B2
公开(公告)日：2014-03-25
申请号：US12637656
申请日：2009-12-14
申请人： Hidehiro Kiyofuji , Tetsuya Iwabuchi , Toshiyuki Kudo , Seiji Kanazawa
发明人： Hidehiro Kiyofuji , Tetsuya Iwabuchi , Toshiyuki Kudo , Seiji Kanazawa
IPC分类号： G01R31/20
CPC分类号： G01R31/2875
摘要： An embodiment of a method for testing an integrated circuit comprises a first step for determining at least one of a group selected from whether or not the chuck top receiving the integrated circuit exists near a probe card which transmits and receives electrical signals to and from the integrated circuit, whether or not the integrated circuit is under testing, and whether or not the probe card has a given temperature, and a second step for adjusting power for heating to be supplied to a heating element provided in the probe card according to the determination result in the first step.
摘要翻译：用于测试集成电路的方法的一个实施例包括第一步骤，用于确定选自接收集成电路的卡盘顶部是否存在于探针卡附近的组中的至少一个，所述探针卡向集成电路发送和接收电信号电路，集成电路是否在测试中，以及探针卡是否具有给定的温度，以及第二步骤，用于根据确定结果调节供应到设置在探针卡中的加热元件的加热功率在第一步。

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