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1. 发明授权

US07278876B2 Connector in which two housings holding a cable are firmly engaged with each other 有权
公开(公告)号：US07278876B2
公开(公告)日：2007-10-09
申请号：US11645467
申请日：2006-12-26
申请人： Seiichi Hara , Kiminobu Tabata , Takeshi Kai , Tsutomu Fukui , Masaru Shinmura , Tadashi Inazu , Shigeki Takahashi
发明人： Seiichi Hara , Kiminobu Tabata , Takeshi Kai , Tsutomu Fukui , Masaru Shinmura , Tadashi Inazu , Shigeki Takahashi
IPC分类号： H01R13/85
CPC分类号： H01R13/582
摘要： A connector includes two housings coupled with each other to hold a cable therebetween. One of the housings includes a cable receiving portion for receiving the cable, a restricting portion adjacent to the cable receiving portion, a first wall portion formed outside the restricting portion and defining a space between the first wall portion and the restricting portion, and a first engaging portion inwardly protruding from the first wall portion. Another of the housings includes a cable pressing portion for pressing the cable towards the cable receiving portion and the restricting portion, and a second wall portion formed adjacent to the cable pressing portion and inserted into the space. The second wall portion has a second engaging portion extending outward from the second wall portion. The first and the second engaging potions are engaged with each other to prevent the housings from separating from each other.

2. 发明申请

US20070167066A1 Connector in which two housings holding a cable are firmly engaged with each other 有权
标题翻译：连接器，其中保持电缆的两个壳体彼此牢固地接合
公开(公告)号：US20070167066A1
公开(公告)日：2007-07-19
申请号：US11645467
申请日：2006-12-26
申请人： Seiichi Hara , Kiminobu Tabata , Takeshi Kai , Tsutomu Fukui , Masaru Shinmura , Tadashi Inazu , Shigeki Takahashi
发明人： Seiichi Hara , Kiminobu Tabata , Takeshi Kai , Tsutomu Fukui , Masaru Shinmura , Tadashi Inazu , Shigeki Takahashi
IPC分类号： H01R13/58
CPC分类号： H01R13/582
摘要： A connector includes two housings coupled with each other to hold a cable therebetween. One of the housings includes a cable receiving portion for receiving the cable, a restricting portion adjacent to the cable receiving portion, a first wall portion formed outside the restricting portion and defining a space between the first wall portion and the restricting portion, and a first engaging portion inwardly protruding from the first wall portion. Another of the housings includes a cable pressing portion for pressing the cable towards the cable receiving portion and the restricting portion, and a second wall portion formed adjacent to the cable pressing portion and inserted into the space. The second wall portion has a second engaging portion extending outward from the second wall portion. The first and the second engaging potions are engaged with each other to prevent the housings from separating from each other.
摘要翻译：连接器包括彼此联接以在其间保持电缆的两个壳体。其中一个壳体包括用于接收电缆的电缆接收部分，与电缆接收部分相邻的限制部分，形成在限制部分外部并限定第一壁部分和限制部分之间的空间的第一壁部分，以及第一接合部从第一壁部向内突出。另一个壳体包括用于将电缆朝向电缆接收部分和限制部分按压的电缆按压部分，以及邻近电缆按压部分形成并插入到该空间中的第二壁部分。第二壁部具有从第二壁部向外延伸的第二接合部。第一和第二接合部分彼此接合以防止壳体彼此分离。

3. 发明授权

US08691302B2 Peptide-containing seasoning 有权
标题翻译：含肽调味料
公开(公告)号：US08691302B2
公开(公告)日：2014-04-08
申请号：US13001931
申请日：2009-07-02
申请人： Takeharu Nakahara , Riichiro Uchida , Yoichi Hanada , Yoshikazu Endo , Shigeki Tanizawa , Hitomi Aota , Seiichi Hara
发明人： Takeharu Nakahara , Riichiro Uchida , Yoichi Hanada , Yoshikazu Endo , Shigeki Tanizawa , Hitomi Aota , Seiichi Hara
IPC分类号： A23L1/238
CPC分类号： A23L27/50
摘要： A fermented seasoning which does not have the bitterness specific to peptides and can be easily ingested orally, in spite of containing large amount of peptides is provided. Namely, a fermented seasoning obtained by adding salt water to an Aspergillus culture or a mixture of the Aspergillus culture with a plant-derived protein material and by controlling the leucine aminopeptidase-I activity in the moromi to be 1.0 U/ml or less, and also controlling the leucine aminopeptidase-II activity in the moromi to be 0.5 U/ml or less, within 10 days after the preparation.
摘要翻译：提供了不含有肽特异性并且可以容易地口服摄取的发酵调味料，尽管含有大量的肽。即，将通过向曲霉菌培养物或曲霉菌培养物的混合物中加入盐水和植物来源的蛋白质材料而得到的发酵调味料，并且通过将茉莉酸中的亮氨酸氨基肽酶-1活性控制为1.0U / ml以下，在制备后10天内，还可以将moromi中的亮氨酸氨基肽酶II活性控制在0.5U / ml以下。

4. 发明授权

US08518665B2 Methods for making 3-indole-pyruvic acid from tryptophan using a tryptophan deaminase 有权
标题翻译：使用色氨酸脱氨酶从色氨酸制备3-吲哚 - 丙酮酸的方法
公开(公告)号：US08518665B2
公开(公告)日：2013-08-27
申请号：US13684888
申请日：2012-11-26
申请人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Yasuaki Takakura , Seiichi Hara , Toshiki Taba , Shunichi Suzuki , Masakazu Sugiyama , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C12P1/00 , C12P21/06
CPC分类号： C12P17/10 , C12N9/0022
摘要： It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.
摘要翻译：本发明的目的是提供一种实现廉价且简单的3-吲哚 - 丙酮酸生产的方法。使用具有编码具有氧化酶活性的蛋白质的特异性核苷酸序列的多核苷酸制备转化体，通过在培养基中培养转化体以在培养基和/或转化体中积累氧化酶而产生氧化酶。此外，在转化体和/或其培养物存在下，将色氨酸转化为3-吲哚 - 丙酮酸，生成3-吲哚 - 丙酮酸。

5. 发明授权

US07736871B2 Peptide-forming enzyme gene 有权
公开(公告)号：US07736871B2
公开(公告)日：2010-06-15
申请号：US11841151
申请日：2007-08-20
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
IPC分类号： C12P21/06 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/00 , C12N9/93 , C12P21/00 , C12P21/02
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

6. 发明授权

US07361458B2 Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
标题翻译：生产α-L-天冬氨酰-L-苯丙氨酸-β-酯的方法及其制备α-天冬氨酰-L-苯丙氨酸-α-甲酯的方法
公开(公告)号：US07361458B2
公开(公告)日：2008-04-22
申请号：US10876673
申请日：2004-06-28
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06 , C12Q1/00 , C12Q1/68
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.
摘要翻译：制备α-L-天冬氨酰基-L-苯丙氨酸-β-酯（也称为α-L-（β-邻位取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L-天冬氨酰基提供了L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;商品名：阿斯巴甜），容易地以高产率和低成本地经历复合合成方法。此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易，便宜且高产量的制备方法。使用具有催化反应能力的酶或含酶物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸生产α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯，其中 L-苯丙氨酸不对L-天冬氨酸-α，β-二酯的β-酯位点进行亲核攻击，但对其α-酯位点进行亲核攻击。

7. 发明授权

US07354746B1 Method for producing optically active IHOG and monatin 失效
标题翻译：用于制备光学活性的IHOG和莫纳甜的方法
公开(公告)号：US07354746B1
公开(公告)日：2008-04-08
申请号：US11609018
申请日：2006-12-11
申请人： Shunichi Suzuki , Seiichi Hara , Toshiki Taba , Yasuaki Takakura
发明人： Shunichi Suzuki , Seiichi Hara , Toshiki Taba , Yasuaki Takakura
IPC分类号： C12P7/42 , C12P21/08 , C12N9/10 , C07H21/04
CPC分类号： C12P17/10 , C12N9/88
摘要： The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and an aldolase which can be used in these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).
摘要翻译：本发明涉及一种用于制备光学活性的IHOG的方法，其可以用于制备莫纳甜。本发明还涉及可用于这些方法的光学活性莫纳甜的制备方法和醛缩酶。因此，本发明能够合成具有高光学纯度的4-（吲哚-3-基甲基）-4-羟基-2-氧代戊二酸，其可用作在光学活性莫纳甜的合成中的吲哚丙酮酸的中间体酸和丙酮酸（或草酰乙酸）。

8. 发明授权

US07288389B2 Peptide-forming enzyme gene 有权
标题翻译：肽形成酶基因
公开(公告)号：US07288389B2
公开(公告)日：2007-10-30
申请号：US10855533
申请日：2004-05-28
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
IPC分类号： C12P21/06 , C12N9/48 , C12N9/52 , C12N1/20 , C07H21/04
CPC分类号： C12N9/00 , C12N9/93 , C12P21/00 , C12P21/02
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
摘要翻译：公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

9. 发明授权

US07052317B2 Connector capable of being firmly fixed to an object and a fixing member used in the connector 有权
标题翻译：能够牢固地固定在物体上的连接器和在连接器中使用的固定部件
公开(公告)号：US07052317B2
公开(公告)日：2006-05-30
申请号：US11092536
申请日：2005-03-29
申请人： Seiichi Hara , Tadashi Ishiwa , Yasufumi Hayashi
发明人： Seiichi Hara , Tadashi Ishiwa , Yasufumi Hayashi
IPC分类号： H01R13/60
CPC分类号： H01R12/7029
摘要： In a connector including an insulator and a contact held by the insulator, a fixing member is coupled to the insulator for fixing the insulator to an object. The fixing member includes a main body received in a receiving portion formed to the insulator. A leg mechanism extends from the main body for being fixed to the object. In addition, a first engaging mechanism extends from the main body and is engaged with the receiving portion in a first direction. On the other hand, the second engaging mechanism extends from the main body and is engaged with the insulator in a second direction opposite to the first direction.
摘要翻译：在包括绝缘体和由绝缘体保持的接触件的连接器中，固定构件联接到绝缘体，以将绝缘体固定到物体上。固定构件包括容纳在形成于绝缘体的容纳部中的主体。腿部机构从主体延伸以固定到物体。此外，第一接合机构从主体延伸并且沿着第一方向与接收部分接合。另一方面，第二接合机构从主体延伸并与第一方向相反的第二方向与绝缘体接合。

10. 发明申请

US20050186654A1 Method for producing dipeptide 失效
标题翻译：二肽生产方法
公开(公告)号：US20050186654A1
公开(公告)日：2005-08-25
申请号：US11008203
申请日：2004-12-10
申请人： Seiichi Hara , Kenzo Yokozeki
发明人： Seiichi Hara , Kenzo Yokozeki
IPC分类号： C12N15/09 , C07K1/06 , C07K5/06 , C12P21/02 , C12P21/06 , C07C237/10 , C07K5/04
CPC分类号： C07K5/06026 , C12P21/02 , Y10S435/823 , Y10S435/849
摘要： A method for producing a dipeptide including the step of exposing a carboxy component and an amine component to an enzyme having an activity to hydrolyze amino acid ester. Such a hydrolase has been found among enzymes in bacteria. The method is useful for producing a peptide simply and inexpensively with a high yield without taking complicate synthetic methods.
摘要翻译：一种制备二肽的方法，包括将羧基组分和胺组分暴露于具有水解氨基酸酯活性的酶的步骤。这种水解酶已经在细菌的酶中发现。该方法可用于在不采取复杂的合成方法的情况下以高产率简单且廉价地生产肽。

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