专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07871810B2 Multiaxis focusing mechanism for microarray analysis 有权
标题翻译：用于微阵列分析的多轴聚焦机制
公开(公告)号：US07871810B2
公开(公告)日：2011-01-18
申请号：US10980886
申请日：2004-11-03
申请人： Scott R. Warren , Louis B. Hoff , Mark F. Oldham
发明人： Scott R. Warren , Louis B. Hoff , Mark F. Oldham
IPC分类号： C12M1/00 , G01N15/06 , G01N21/47
CPC分类号： G06K9/00127 , G06K9/32
摘要： Systems and methods for positioning a multi-featured biological array relative to a signal acquisition device. Detection of the array's positional deviation may be achieved by a calibration beam reflected from the array surface and detected by a position sensitive detector (PSD). The PSD-measured positional deviation can be transformed and used in a control loop to correct for positional variations of the array. The calibration beam and PSD may also be used to detect the array or feature boundaries, thereby allowing lateral centering or positioning of the array relative to the signal acquisition device.
摘要翻译：用于相对于信号采集装置定位多功能生物阵列的系统和方法。通过从阵列表面反射并由位置敏感检测器（PSD）检测的校准光束可以实现阵列位置偏差的检测。可以将PSD测量的位置偏差变换并用于控制回路中以校正阵列的位置变化。校准光束和PSD也可用于检测阵列或特征边界，从而允许阵列相对于信号采集装置的侧向对中或定位。

2. 发明授权

US06744502B2 Shaped illumination geometry and intensity using a diffractive optical element 失效
$Shaped illumination geometry and intensity using a diffractive optical element$
标题翻译：使用衍射光学元件形成照明几何形状和强度
公开(公告)号：US06744502B2
公开(公告)日：2004-06-01
申请号：US09964778
申请日：2001-09-28
申请人： Louis B. Hoff , Mark F. Oldham
发明人： Louis B. Hoff , Mark F. Oldham
IPC分类号： G01J330
CPC分类号： G02B27/0944 , G01J1/20 , G01J3/10 , G02B19/0014 , G02B19/0052 , G02B27/0927
摘要： A method and apparatus to illuminate a target. The apparatus can comprise a first lens configured to receive light from the light source, a diffractive optical element configured to receive the light from the first lens and to regulate the light into regulated light, and second lens configured to receive the regulated light and to direct the regulated light to a selected area of the target.
摘要翻译：照明目标的方法和装置。该装置可以包括被配置为从光源接收光的第一透镜，被配置为接收来自第一透镜的光并将光调节为调节光的衍射光学元件，以及被配置为接收调节光并引导调节光到目标的选定区域。

3. 发明授权

US07006215B2 Shaped illumination geometry and intensity using a diffractive optical element 失效
$Shaped illumination geometry and intensity using a diffractive optical element$
标题翻译：使用衍射光学元件形成照明几何形状和强度
公开(公告)号：US07006215B2
公开(公告)日：2006-02-28
申请号：US10837616
申请日：2004-05-04
申请人： Louis B. Hoff , Mark F. Oldham
发明人： Louis B. Hoff , Mark F. Oldham
IPC分类号： G01J3/30
CPC分类号： G02B27/0944 , G01J1/20 , G01J3/10 , G02B19/0014 , G02B19/0052 , G02B27/0927
摘要： A method and apparatus to illuminate a target. The apparatus can comprise a first lens configured to receive light from the light source, a diffractive optical element configured to receive the light from the first lens and to regulate the light into regulated light, and second lens configured to receive the regulated light and to direct the regulated light onto a selected area of the target.
摘要翻译：照明目标的方法和装置。该装置可以包括被配置为从光源接收光的第一透镜，被配置为接收来自第一透镜的光并将光调节为调节光的衍射光学元件，以及被配置为接收调节光并引导调节光到目标的选定区域。

4. 发明授权

US5543026A Real-time scanning fluorescence electrophoresis apparatus for the analysis of polynucleotide fragments 失效
标题翻译：实时扫描荧光电泳仪用于分析多核苷酸片段
公开(公告)号：US5543026A
公开(公告)日：1996-08-06
申请号：US192485
申请日：1994-02-07
申请人： Louis B. Hoff , Eric W. Lachenmeier , Yefim M. Raysberg , Eric S. Nordman
发明人： Louis B. Hoff , Eric W. Lachenmeier , Yefim M. Raysberg , Eric S. Nordman
IPC分类号： G01N21/64 , G01N27/447 , G01N33/68 , C02F1/40 , C02F11/00 , C25B9/00 , C25B11/00
CPC分类号： G01N27/44721
摘要： This invention relates to an improved real-time scanning fluorescence electrophoresis apparatus for the electrophoretic analysis of fluorescently-labeled polynucleotide fragments. The apparatus is characterized by having an electrophoresis chamber containing an electrophoretic separation medium capable of accommodating multiple electrophoresis lanes arranged in a planar array, a fluorescence detector mounted on a translatable stage, a light source for exciting fluorescent molecules, and a computer for collecting data consisting of time, location, fluorescence wavelength and fluorescent intensity information. The improvements herein disclosed include, (i) using a spectral-array detector for detecting the emission light from the fluorescently-labeled polynucleotide fragments including the simultaneous detection of multiple fluorescent labels, and, (ii) a temperature control means to control the temperature of the electrophoretic separation medium during electrophoresis.
摘要翻译：本发明涉及用于荧光标记的多核苷酸片段的电泳分析的改进的实时扫描荧光电泳装置。该装置的特征在于具有包含能够容纳布置在平面阵列中的多个电泳泳道的电泳分离介质的电泳室，安装在可平移台上的荧光检测器，用于激发荧光分子的光源，以及用于收集数据的计算机，的时间，位置，荧光波长和荧光强度信息。本文公开的改进包括：（i）使用光谱阵列检测器来检测来自荧光标记的多核苷酸片段的发射光，包括同时检测多个荧光标记，和（ii）控制温度的温度控制装置电泳过程中的电泳分离介质。

5. 发明授权

US5443791A Automated molecular biology laboratory 失效
标题翻译：自动分子生物学实验室
公开(公告)号：US5443791A
公开(公告)日：1995-08-22
申请号：US927254
申请日：1992-08-07
申请人： G. Richard Cathcart , Thomas Brennan-Marquez , John A. Bridgham , George S. Golda , Harry A. Guiremand , Marianne Hane , Louis B. Hoff , Eric Lachenmeier , Melvyn N. Kronick , Douglas H. Keith , Paul E. Mayrand , Michael L. Metzker , William J. Mordan , Lincoln J. McBride , John Shigeura , Chen-Hanson Ting , Norman M. Whiteley
发明人： G. Richard Cathcart , Thomas Brennan-Marquez , John A. Bridgham , George S. Golda , Harry A. Guiremand , Marianne Hane , Louis B. Hoff , Eric Lachenmeier , Melvyn N. Kronick , Douglas H. Keith , Paul E. Mayrand , Michael L. Metzker , William J. Mordan , Lincoln J. McBride , John Shigeura , Chen-Hanson Ting , Norman M. Whiteley
IPC分类号： B01L3/14 , G01N35/00 , G01N35/02 , G01N35/04 , G01N35/10 , G01N21/01 , G01N1/10 , G01N21/11 , G01N35/08
CPC分类号： G01N35/0099 , B01L3/50825 , G01N35/0098 , B01F2215/0037 , G01N2035/00079 , G01N2035/00237 , G01N2035/00356 , G01N2035/00435 , G01N2035/0403 , G01N2035/0475 , G01N2035/0491 , G01N2035/1013 , G01N2035/102 , G01N2035/1034 , G01N2035/1058 , G01N2035/106 , G01N35/028 , G01N35/1004 , G01N35/109 , Y10S435/809 , Y10T436/119163 , Y10T436/2575
摘要： A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for programming and editing. A gauge block registered on the worksurface provides for calibration using the sensing tip, and register cavities on the worksurface provide for modular stations. There is a wash station fop the pipette tip on the worksurface. An automated laboratory based on the liquid-handling system has heating and cooling and a sealable incubation station as well as a magnetic separation station. Methods are disclosed using the apparatus to convey droplets of liquid, to aspirate with minimum tip contamination, to mix liquids in containers, and to validate the worksurface. A duck-billed closure is disclosed for minimizing evaporation and cross-contamination during processing, and is a part of a container disclosed for storing and transporting liquids.
摘要翻译：液体处理仪器具有工作表面，其具有用于模块化站的注册以支持液体容器，具有连接到感测电路的移液管尖端的移液管装置，用于移动吸头的机器人翻译系统以及具有标识用户界面的控制系统用于编程和编辑。在工作表面上注册的量块提供了使用感应尖端的校准，工件表面上的注册腔提供模块化站。在工作台上有一个洗手台，用于吸取吸头。基于液体处理系统的自动化实验室具有加热和冷却以及可密封的孵化站以及磁选机。公开了使用装置输送液体的方法，以最小的尖端污染抽吸以将容器中的液体混合并验证工作面。公开了一种用于最小化加工期间的蒸发和交叉污染的鸭嘴盖，并且是用于储存和运输液体的容器的一部分。

6. 发明授权

US5798032A Method and apparatus for automated carbohydrate mapping and sequencing 失效
标题翻译：自动碳水化合物测绘和测序的方法和设备
公开(公告)号：US5798032A
公开(公告)日：1998-08-25
申请号：US558047
申请日：1995-11-13
申请人： Shaheer H. Khan , Roger A. O'Neill , Louis B. Hoff
发明人： Shaheer H. Khan , Roger A. O'Neill , Louis B. Hoff
IPC分类号： G01N27/447 , C25B7/00 , C25B9/00
CPC分类号： G01N27/44773 , G01N27/44743
摘要： Methods and apparatus are disclosed for improved mapping and sequencing of carbohydrate polymers. In one aspect, the improvement includes a means for coupling two capillary electrophoresis (CE) tubes in such a way so as to (i) efficiently transfer a selected sample component from a first CE capillary to a second CE capillary or, (ii) introduce a supplementary reagent into the separation path between the two capillaries, e.g., an internal standard, binding agent, enzyme, and the like. In an additional aspect, the invention includes improved apparatus and methods for electrophoresis of labeled carbohydrates in which a sample carbohydrate labeled with a first label is separated by CE and its migration behavior is compared with an internal standard labeled with one or more second labels which are distinguishable from the first label. In yet another aspect, the invention includes improved method and apparatus for sequencing carbohydrates in which a sample mixture is separated in a first CE dimension, a component of the sample mixture is selected, the selected component is incubated with an enzyme reagent, the reaction products are separated in a second CE dimension, the electrophoretic behavior of the reaction products is correlated with the known specificity of the enzyme reagent, and the process is repeated to determine the complete sequence of the carbohydrate sample.
摘要翻译：公开了用于改进碳水化合物聚合物的测绘和测序的方法和装置。在一个方面，改进包括以这样的方式耦合两个毛细管电泳（CE）管的方法，以便（i）有效地将选定的样品组分从第一CE毛细管转移到第二CE毛细管，或者（ii）引入在两个毛细管之间的分离路径中的补充试剂，例如内标，结合剂，酶等。在另一方面，本发明包括用于电泳标记的碳水化合物的改进的装置和方法，其中用CE标记的样品碳水化合物被CE分离，并将其迁移行为与标记有一个或多个第二标记的内标进行比较与第一个标签区分开来。另一方面，本发明包括用于测序碳水化合物的改进方法和装置，其中样品混合物在第一CE维度上分离，选择样品混合物的组分，将所选择的组分与酶试剂一起温育，反应产物在第二CE维度上分离，反应产物的电泳行为与酶试剂的已知特异性相关，并重复该过程以确定碳水化合物样品的完整序列。

7. 发明授权

US5338426A Horizontal polyacrylamide gel electrophoresis 失效
标题翻译：卧式聚丙烯酰胺凝胶电泳
公开(公告)号：US5338426A
公开(公告)日：1994-08-16
申请号：US936979
申请日：1992-08-28
申请人： John Shigeura , John A. Bridgham , Louis B. Hoff , P. Eric Mayrand
发明人： John Shigeura , John A. Bridgham , Louis B. Hoff , P. Eric Mayrand
IPC分类号： G01N27/447 , C25B9/00
CPC分类号： G01N27/44704
摘要： An electrophoresis apparatus has a gel film cast between two plates and buffer reservoirs at each end of the film with electrodes connectable to an external power supply for providing electromotive force for driving electrophoresis. The reservoirs are configured to wet the ends of the gel film and submerge the electrodes with the apparatus positioned either horizontally or vertically, so gel films can be cast horizontally with sample wells formed in the end of the gel between the plates. Samples may be added to the wells and run into the gel with the apparatus positioned vertically, and the analytical separation may be performed with the apparatus again positioned horizontally, such as in an automatic scanning apparatus.
摘要翻译：电泳装置具有在两个板之间的凝胶膜和在膜的每个端部的缓冲储存器，其电极可连接到外部电源，以提供用于驱动电泳的电动势。储存器被配置为润湿凝胶膜的端部并使设备浸没在水平或垂直方向上，因此凝胶膜可以在形成在凝胶的端部之间的样品阱之间水平地铸造。可以将样品加入到孔中，并使装置垂直放置在凝胶中，并且可以在水平再次定位的装置上进行分析分离，例如在自动扫描装置中。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式