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1. 发明授权

US5730850A Capillary array electrophoresis system 失效
标题翻译：毛细管阵列电泳系统
公开(公告)号：US5730850A
公开(公告)日：1998-03-24
申请号：US588776
申请日：1996-01-19
申请人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada , Yoshinobu Kohara
发明人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada , Yoshinobu Kohara
IPC分类号： G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2035/00237
摘要： A capillary array electrophoresis system by which measurement is conducted using a large number of capillaries. The electrophoresis system includes a plurality of capillary array sheets stacked one on top of another wherein end portions of the capillaries at a detection region are arranged two-dimensionally in such a manner as to elute two-dimensionally a sample from the distal end of each capillary. Excitation light is applied to the sample eluted into a buffer solution, and a two-dimensional fluorescent image is picked up by a detector.
摘要翻译：使用大量毛细管进行测量的毛细管阵列电泳系统。所述电泳系统包括多个毛细管阵列片，所述多个毛细管阵列片层叠在另一个之上，其中毛细管在检测区域的端部以二维排列，从每个毛细管的末端二维地抽出样品。将激发光施加到洗脱到缓冲溶液中的样品上，并通过检测器拾取二维荧光图像。

2. 发明授权

US6048444A Capillary electrophoresis apparatus 失效
标题翻译：毛细管电泳仪
公开(公告)号：US6048444A
公开(公告)日：2000-04-11
申请号：US979542
申请日：1997-11-26
申请人： Satoshi Takahashi , Hideki Kambara , Takashi Yamada
发明人： Satoshi Takahashi , Hideki Kambara , Takashi Yamada
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721
摘要： A capillary electrophoresis apparatus of the invention has: a plurality of capillaries which are filled with a migration medium and have first ends into which samples are injected and second ends in which components included in the samples are eluted; a sheath flow cell in which the second ends are arranged in a straight line at first predetermined intervals and are terminated and a sheath flow is formed; a buffer solution vessel for housing a buffer solution flowing in the sheath flow cell; a drain vessel housing the buffer solution flowed from the sheath flow cell; an optical system emitting laser light to a part near the second ends; and a fluorescent detection system for detecting fluorescent light generated from fluorophore labelling the components included in the sample eluted near the second ends by the emission of laser light, wherein the buffer solution flows from the lower part to the upper part of the sheath flow cell, thereby forming a sheath flow in the sheath flow cell.
摘要翻译：本发明的毛细管电泳装置具有：填充有迁移介质的多个毛细管，其具有注入样品的第一端和样品中包含的成分被洗脱的第二端; 鞘流池，其中第二端以第一预定间隔以直线布置并且终止并形成鞘流; 用于容纳在鞘流池中流动的缓冲溶液的缓冲溶液容器; 容纳从鞘流池流出的缓冲溶液的排水管; 将激光发射到靠近第二端的部分的光学系统; 以及荧光检测系统，用于检测由荧光团产生的荧光，其中通过发射激光来标记在第二端附近洗脱的样品中包含的成分，其中缓冲溶液从鞘流动池的下部流向上部，从而在鞘流动池中形成鞘流。

3. 发明授权

US5968331A Sample holding device for electrophoresis apparatus and sample injection method 失效
标题翻译：电泳仪样品保持装置及样品注射法
公开(公告)号：US5968331A
公开(公告)日：1999-10-19
申请号：US554808
申请日：1995-11-07
申请人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada
发明人： Hideki Kambara , Satoshi Takahashi , Takashi Anazawa , Takashi Yamada
IPC分类号： G01N33/50 , G01N27/447 , G01N27/26
CPC分类号： G01N27/44743
摘要： A sample holding device for electrophoresis apparatus according to the present invention wherein a plurality of sample holding capillaries are laid out in an array and are immobilized to the supporting jig. This sample holding device is configured to ensure the lower ends of the capillaries can contact the sample injection portion of the electrophoresis separation part of the electrophoresis apparatus, and provides easy sample injection and prevents the gel capillaries of the electrophoresis separation part from being damaged when sample holding capillaries are filled with gels, thereby allowing repeated use of the gel capillaries of the electrophoresis separation part.
摘要翻译：根据本发明的用于电泳装置的样品保持装置，其中多个样品保持毛细管以阵列布置并固定在支撑夹具上。该样品保持装置被配置为确保毛细血管的下端可以接触电泳装置的电泳分离部的样品注入部，并且提供容易的样品注射，并且防止当样品时电泳分离部分的凝胶毛细血管损伤保持毛细血管填充凝胶，从而允许重复使用电泳分离部分的凝胶毛细血管。

4. 发明授权

US5534703A Electrophoresis analyzer with wavelength selective detection 失效
标题翻译：具有波长选择性检测的电泳分析仪
公开(公告)号：US5534703A
公开(公告)日：1996-07-09
申请号：US220261
申请日：1994-03-30
申请人： Hideki Kambara , Takashi Anazawa , Satoshi Takahashi , Katsuhiko Murakami
发明人： Hideki Kambara , Takashi Anazawa , Satoshi Takahashi , Katsuhiko Murakami
IPC分类号： G01J3/427 , G01N21/27 , G01N21/64 , G01N27/447 , G01N27/26
CPC分类号： G01N21/6456 , G01N27/44721
摘要： An electrophoresis analyzer includes a light detector for detecting images of a linear fluorescence emitting region, the linear fluorescence emitting region including a plurality of fluorescence emitting points disposed along a straight line, each of the fluorescence emitting points being a point at which samples emit light including a plurality of wavelengths, the light detector including a line sensor, the line sensor including a plurality of photopixels disposed along a straight line, the photopixels constituting a detection surface of the line sensor, an image forming unit for receiving the light emitted by the samples at the fluorescence emitting points and forming a plurality of images of each of the fluorescence emitting points on the detection surface of the line sensor at mutually different positions disposed at a predetermined interval along the straight line along which the photopixels are disposed, and a filter unit disposed between the linear fluorescence emitting region and the light detector for selectively filtering the light emitted by the samples at the fluorescence emitting points such that the images of each of the fluorescence emitting points are formed by light including mutually different ones of the wavelengths.
摘要翻译：电泳分析仪包括用于检测线性荧光发射区域的图像的光检测器，所述线性荧光发射区域包括沿着直线设置的多个荧光发射点，每个荧光发射点是样品发射的点，包括多个波长，所述光检测器包括线传感器，所述线传感器包括沿着直线设置的多个照相像素，构成所述线传感器的检测表面的所述光像素，用于接收由所述样本发射的光的图像形成单元在荧光发射点处，并且沿着沿着设置有像素的直线以预定间隔设置的相互不同的位置，在线传感器的检测表面上形成每个荧光发射点的多个图像，以及滤光器单元设置在线性荧光发射区域a之间 nd光检测器，用于在荧光发射点选择性地过滤由样品发射的光，使得每个荧光发射点的图像由包括相互不同的波长的光形成。

5. 发明授权

US5938908A Capillary array electrophoresis system 失效
标题翻译：毛细管阵列电泳系统
公开(公告)号：US5938908A
公开(公告)日：1999-08-17
申请号：US847735
申请日：1997-04-22
申请人： Takashi Anazawa , Hideki Kambara , Satoshi Takahashi , Kazumichi Imai , Hisanori Nasu
发明人： Takashi Anazawa , Hideki Kambara , Satoshi Takahashi , Kazumichi Imai , Hisanori Nasu
IPC分类号： G01N21/64 , B01D57/02 , G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44721 , G01N27/44782 , G01N2035/00237
摘要： A capillary electrophoresis system comprising a plurality of capillaries filled with a migration medium in which samples to which fluorephore labels are added migrate, a light source providing an excitation light exciting the fluorephore labels, a photo detector detecting a fluorescence radiated from the fluorephore labels, and light focusing means placed between the plurality of capillaries to which the excitation light is irradiated. The parts of the plurality of capillaries to which the excitation light is irradiated and the light focusing means are arranged in a plane shape. The excitation light is irradiated through the plurality of capillaries and the light focusing means, the capillaries and light focusing means alternatively placed in the parts where an excitation light is irradiated. The light focusing means consisting of cylindrical rod lenses. The axis of the cylindrical rod lenses is placed substantially parallel with the capillaries, which can detect the fluorescence from samples by irradiating a plurality of capillaries substantially at the same time in a batch without mechanical scanning of a plurality of capillaries or without optical scanning of a light beam, providing a highly sensitive detection of samples.
摘要翻译：一种毛细管电泳系统，包括填充有迁移介质的多个毛细管，其中添加有荧光标记的样品迁移，提供激发荧光标记的激发光的光源，检测从荧光标记物辐射的荧光的光电检测器，以及放置在激发光照射到的多个毛细管之间的光聚焦装置。照射激发光的多个毛细管的部分和光聚焦装置被布置成平面形状。激发光通过多个毛细管和光聚焦装置照射，毛细管和光聚焦装置交替地放置在照射激发光的部分中。光聚焦装置由圆柱形棒状透镜组成。圆柱形棒状透镜的轴线基本上与毛细管平行放置，其可以通过基本上同时照射多个毛细管而检测来自样品的荧光，而不需要对多个毛细管进行机械扫描，或者不进行光学扫描光束，提供高灵敏度的样品检测。

6. 发明授权

US06224733B1 DNA detector and DNA detection method 失效
标题翻译： DNA检测器和DNA检测方法
公开(公告)号：US06224733B1
公开(公告)日：2001-05-01
申请号：US09400438
申请日：1999-09-21
申请人： Satoshi Takahashi , Hideki Kambara
发明人： Satoshi Takahashi , Hideki Kambara
IPC分类号： G01N2726
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2035/00237
摘要： An electrophoresis apparatus includes a plurality of capillaries through which samples labeled with a plurality of fluorophores migrate, a pair of electrodes which generate an electric field which causes the samples labeled with the fluorophores to migrate through the capillaries, a light source which emits laser light which irradiates the samples labeled with the fluorophores at irradiation positions respectively corresponding to the capillaries in a direction which is perpendicular to a direction in which the samples migrate and which is parallel to a plane formed by the capillaries, thereby exciting the fluorophores to emit fluorescence at the irradiation positions, a photodetector which detects the fluorescence emitted by the fluorophores, and a holder which holds the capillaries at intervals of 1 mm or less near the irradiation positions.
摘要翻译：电泳装置包括多个毛细管，多个荧光团标记的样品通过该毛细管迁移，产生电场的一对电极，使得用荧光团标记的样品迁移穿过毛细管;发出激光的光源，在分别对应于毛细管的照射位置处以与样品迁移的方向垂直的方向照射荧光团的样品并且平行于由毛细管形成的平面，从而激发荧光团以在荧光团上发射荧光照射位置，检测由荧光团发出的荧光的光电检测器，以及在照射位置附近以1mm或更小的间隔保持毛细管的保持器。

7. 发明授权

US6156177A DNA detector and DNA detection method 失效
标题翻译： DNA检测器和DNA检测方法
公开(公告)号：US6156177A
公开(公告)日：2000-12-05
申请号：US525024
申请日：2000-03-14
申请人： Satoshi Takahashi , Hideki Kambara
发明人： Satoshi Takahashi , Hideki Kambara
IPC分类号： G01N27/447 , G01N35/00 , G01N27/26
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2035/00237
摘要： A method for analyzing samples includes the steps of introducing samples labeled with fluorophores into a first end of each of a plurality of electrophoresis lanes including a separation medium, a second end of each of the electrophoresis lanes being disposed in an optical cell filled with a buffer solution, applying an electric field to the electrophoresis lanes to separate the samples labeled with the fluorophores and cause the separated samples labeled with the fluorophores to migrate through the electrophoresis lanes into the buffer solution in the optical cell, exciting, with light from a light source, the fluorophores labeling the separated samples in the buffer solution in the optical cell to emit fluorescence in the buffer solution in the optical cell, and detecting, with a photodetector, the fluorescence emitted by the fluorophores labeling the separated samples in the buffer solution in the optical cell. The light source is selected from the group consisting of a He--Ne laser which emits light having a wavelength of 594 nm, a He--Ne laser which emits light having a wavelength of 632.8 nm, a YAG laser which emits light having a wavelength of 532 nm, and a semiconductor laser which emits light having a wavelength of 670 nm.
摘要翻译：分析样品的方法包括以下步骤：将荧光团标记的样品引入到包括分离介质的多个电泳泳道的每一个的第一端中，每个电泳通道的第二端设置在填充有缓冲液的光学池中溶液，将电场施加到电泳通道以分离用荧光团标记的样品，并使分离的用荧光团标记的样品通过电泳泳道迁移到光学池中的缓冲溶液中，用来自光源的光荧光团将分离的样品标记在光学单元中的缓冲溶液中以在光学单元中的缓冲溶液中发射荧光，并用光电检测器检测由荧光团发射的荧光，将分离的样品标记在缓冲溶液中的分离样品中光电池。光源选自发射波长为594nm的光的He-Ne激光器，发射波长为632.8nm的光的He-Ne激光器，发射波长为 532nm的激光，以及发射波长为670nm的光的半导体激光器。

8. 发明授权

US5824481A DNA analyzing method 失效
标题翻译： DNA分析方法
公开(公告)号：US5824481A
公开(公告)日：1998-10-20
申请号：US812698
申请日：1997-03-06
申请人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
发明人： Hideki Kambara , Kazunori Okano , Satoshi Takahashi , Keiichi Nagai , Kazuko Kawamoto , Hiroko Furuyama
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6846
摘要： A DNA analyzing method which bonds a first oligomer of known base sequence to a DNA fragment obtained by digesting a DNA sample with a restrictive enzyme. The oligomer and DNA fragment are hybridized to other oligomers which have the sequences of all combinations of the types of bases within the length of several bases following the known base sequence. The presence or absence of hybridization or complementary DNA strand extension is determined and identifies the DNA fragment terminal sequence from this result. The DNA fragments are then fractionated and analyzed to determine the sequence. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.
摘要翻译：将已知碱基序列的第一寡聚体与限制性酶消化DNA样品获得的DNA片段结合的DNA分析方法。寡聚体和DNA片段与其它寡核苷酸杂交，其具有在已知碱基序列之后的几个碱基长度内的碱基类型的所有组合的序列。确定是否存在杂交或互补DNA链延伸，并从该结果鉴定DNA片段末端序列。然后将DNA片段分级并分析以确定序列。该DNA分析方法提供了长DNA或DNA片段的混合物的有效分析。

9. 发明授权

US5414508A Optical cell and optical detection systems light absorption 失效
标题翻译：光电池和光学检测系统的光吸收
公开(公告)号：US5414508A
公开(公告)日：1995-05-09
申请号：US25232
申请日：1993-03-02
申请人： Satoshi Takahashi , Hideki Kambara
发明人： Satoshi Takahashi , Hideki Kambara
IPC分类号： G01N21/05 , G01N21/03 , G01N27/447 , G01N30/74 , G01N15/02
CPC分类号： G01N21/0303 , G01N2021/0346 , G01N2021/0392 , G01N21/031 , G01N21/05
摘要： An optical cell or an optical detection system, of a simple structure having a small light path length, such as, for example, an optical cell having a light path length equal to that of a capillary. Light incidence and exit surfaces of the optical cell are each formed by a half transmitting mirror, and light is made incident on the optical cell in a direction perpendicular to the cell. The reflectance of the half transmitting mirror formed at the incidence surface is set smaller than that of the half transmitting mirror formed at the exit surface. The said optical cell or optical detection system is used as an optical detector portion of a sample separation and detection system which is for separation and detection of a sample using a liquid chromatography system or an electrophoresis system. Because of multi-reflection of light between both half transmitting mirrors, an effective increase of absorbance is obtained and even a sample whose absorbance is not higher than the noise level can be detected easily in high sensitivity. Further, since even a low concentration of sample does not cause lowering in the intensity of fluorescence, it is possible to detect both absorbance and fluorescence simultaneously.
摘要翻译：具有光路长度小的简单结构的光学单元或光学检测系统，例如具有与毛细管相同的光程长度的光学单元。光学单元的光入射面和出射面各自由半透射镜形成，并且使光在垂直于单元的方向入射到光学单元上。形成在入射面处的半透射镜的反射率被设定为小于形成在出射面处的半透射镜的反射率。所述光学单元或光学检测系统用作样品分离和检测系统的光学检测器部分，其用于使用液相色谱系统或电泳系统分离和检测样品。由于两个半透射反射镜之间的光的多次反射，所以可以有效地增加吸光度，并且甚至可以容易地以高灵敏度检测吸光度不高于噪声水平的样品。此外，由于即使低浓度的样品不会引起荧光强度的降低，也可以同时检测吸光度和荧光。

10. 发明授权

US06576108B1 DNA detector and DNA detection method 失效
标题翻译： DNA检测器和DNA检测方法
公开(公告)号：US06576108B1
公开(公告)日：2003-06-10
申请号：US09524269
申请日：2000-03-13
申请人： Satoshi Takahashi , Hideki Kambara
发明人： Satoshi Takahashi , Hideki Kambara
IPC分类号： G01N2726
CPC分类号： G01N27/44782 , G01N27/44721 , G01N2035/00237
摘要： An electrophoresis apparatus includes an optical cell having an outlet for fluid and filled with a buffer solution, and a plurality of electrophoresis lanes, each including a separation medium, which separate samples labeled with fluorophores, one end of each of the electrophoresis lanes being disposed in the optical cell to enable the separated samples labeled with the fluorophores to migrate from the electrophoresis lanes into the buffer solution in the optical cell. There is also provided a vessel containing a buffer solution, the vessel being disposed so that a level of the buffer solution in the vessel is higher than the ends of the electrophoresis lanes disposed in the optical cell, a passage connecting the vessel with the optical cell, a light source which emits light which excites the fluorophores to emit fluorescence, and a photodetector which detects the fluorescence emitted by the fluorophores.
摘要翻译：电泳装置包括具有流体出口并填充有缓冲溶液的光学单元和多个电泳通道，每个电泳通道均包含分离介质，分离介质分离出用荧光团标记的样品，每个电泳通道的一端设置在光电池使得用荧光团标记的分离样品从电泳泳道迁移到光学池中的缓冲溶液中。还提供了一种容纳缓冲溶液的容器，该容器被设置成使得容器中的缓冲溶液的水平高于设置在光学单元中的电泳通道的端部，连接容器与光学单元的通道发出激发荧光团发出荧光的光的光源，以及检测由荧光团发出的荧光的光检测器。

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