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    • 10. 发明授权
    • Process for separating porphyrins
    • 分离卟啉的方法
    • US4436663A
    • 1984-03-13
    • US404054
    • 1982-08-02
    • Kenji MaruhashiIchiro KojimaYutaka OguchiNoboru EndohTetsuo Satoh
    • Kenji MaruhashiIchiro KojimaYutaka OguchiNoboru EndohTetsuo Satoh
    • C12P17/06B01J20/26B01J20/34C07D487/22C12P17/18C12R1/06
    • C12P17/182B01J20/26
    • A process for separating porphyrins, which comprises(1) adjusting the pH of the liquid phase of a culture broth containing at least two porphyrins to about 2.5 to about 4 and collecting the resulting solid containing the porphyrins,(2) either (a) preparing an acidic aqueous solution of said solid and adjusting the pH of its liquid phase left after removal of insoluble matter to about 6 to about 8 to form an aqueous solution containing the porphyrins, or (b) preparing an acid-containing lower alcohol solution of said solid, adjusting the pH of its liquid phase left after removal of insoluble matter, adding water in an amount at least twice the amount of the solution to precipitate lower alkyl esters of the porphyrins, collecting the precipitates and dissolving them in an organic solvent to form an organic solvent solution of the porphyrin esters,(3) contacting the solution obtained in (a) or (b) in step (2) with a copolymer having a surface area of at least about 700 m.sup.2 /g and derived from(i) divinylbenzene,(ii) styrene or its functional derivative,(iii) an alkenyl ester of a benzenepolycarboxylic acid represented by the following formula ##STR1## wherein R represents an alkenyl group having 3 to 10 carbon atoms, and n is 2 or 3,thereby permitting adsorption of the porphyrins or their esters to the copolymer, and(4) thereafter, eluting the copolymer to obtain fractions of the individual porphyrins or their esters; and an adsorbent for use in the above-mentioned process.
    • 一种分离卟啉的方法,其包括(1)将含有至少两个卟啉的培养液的液相pH调节至约2.5至约4并收集所得到的含有卟啉的固体,(2)制备 将所述固体的酸性水溶液调节其去除不溶物后留下的液相的pH至约6至约8以形成含有卟啉的水溶液,或(b)制备所述含贱金属的低级醇溶液 固体,调节去除不溶物后留下的液相的pH值,加入至少两倍于溶液量的水,以沉淀卟啉的低级烷基酯,收集沉淀物并将其溶解在有机溶剂中形成 卟啉酯的有机溶剂溶液,(3)使步骤(2)中(a)或(b)中获得的溶液与表面积至少为约700m 2 / g的共聚物接触,得到 (i)二乙烯基苯,(ii)苯乙烯或其官能衍生物,(iii)由下式表示的苯多羧酸的链烯基酯,其中R表示碳原子数3〜10的烯基,n为2 或3,从而允许将卟啉或其酯吸附到共聚物上,和(4)此后,将共聚物洗脱得到各个卟啉或其酯的部分; 和用于上述方法的吸附剂。