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1. 发明授权

US08383385B2 Variants of glycoside hydrolases 有权
标题翻译：糖苷水解酶的变体
公开(公告)号：US08383385B2
公开(公告)日：2013-02-26
申请号：US13009524
申请日：2011-01-19
申请人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12N9/42 , C12S3/00 , C12S3/02 , C12P19/14 , C11D3/386
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

2. 发明授权

US07932073B2 Variants of glycoside hydrolases 有权
标题翻译：糖苷水解酶的变体
公开(公告)号：US07932073B2
公开(公告)日：2011-04-26
申请号：US11891249
申请日：2007-08-08
申请人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Connie Ward , Joel Cherry , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12N9/42 , C12S3/00 , C11D3/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

3. 发明申请

US20050048619A1 Variants of glycoside hydrolases 审中-公开
标题翻译：糖苷水解酶的变体
公开(公告)号：US20050048619A1
公开(公告)日：2005-03-03
申请号：US10926223
申请日：2004-08-25
申请人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12N9/42 , C07H21/04 , C12N9/24 , C12P21/04
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176 , Y02E50/16
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

4. 发明申请

US20110136167A1 Variants of glycoside hydrolases 有权
公开(公告)号：US20110136167A1
公开(公告)日：2011-06-09
申请号：US13009524
申请日：2011-01-19
申请人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C12P1/00 , C12N9/24 , C11D3/386
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

5. 发明申请

US20080167214A1 Variants of glycoside hydrolases 有权
标题翻译：糖苷水解酶的变体
公开(公告)号：US20080167214A1
公开(公告)日：2008-07-10
申请号：US11891249
申请日：2007-08-08
申请人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
发明人： Sarah Teter , Joel Cherry , Connie Ward , Aubrey Jones , Paul Harris , Jung Yi
IPC分类号： C11D3/386 , C12N9/24 , C07H21/04 , C12N15/63 , C12N5/06 , C12P21/02 , B09C1/10
CPC分类号： C12N9/2437 , C11D3/38645 , C12N9/2434 , C12N9/2445 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01021 , C12Y302/01091 , C12Y302/01176
摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译：本发明涉及母体糖苷水解酶的变体，其包含在对应于位置21,94,157,205,206,247,337,350,373,383,438,455,467的一个或多个位置的取代，和 486，SEQ ID NO：2的氨基酸1至513，并且任选地还包含在对应于位置8,22,41,49,57,113,193,196,226,227,246,29的一个或多个位置的取代， SEQ ID NO：2的氨基酸1至513的251,255,259,301,356,371,411和462在对应于位置8,22,41,49,57,113， SEQ ID NO：2的氨基酸1至513的193,196,226,227,246,251,255,259,301,356,371,411和462，其中所述变体具有糖苷水解酶活性。本发明还涉及编码变体糖苷水解酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。

6. 发明授权

US09045748B2 Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权
标题翻译：用DNA文库转化和表达筛选丝状真菌细胞的方法
公开(公告)号：US09045748B2
公开(公告)日：2015-06-02
申请号：US11140150
申请日：2005-05-26
申请人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
发明人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
IPC分类号： C12N15/10 , C12N15/80 , C40B40/02
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.
摘要翻译：本发明涉及丝状真菌转化体的表达筛选方法，其包括：（a）分离导入大肠杆菌的DNA文库的单个菌落转化体; （b）从每个单个菌落的大肠杆菌转化体制备DNA; （c）将步骤（b）的每种DNA制剂的样品引入到丝状真菌的原生质体的分离悬浮液中以获得其转化体，其中每个转化体含有来自DNA文库的单个多核苷酸的一个或多个拷贝; （d）在选择性生长培养基上生长步骤（c）的单个丝状真菌转化体，从而允许丝状真菌转化体生长，同时抑制未转化的丝状真菌的生长; 和（e）测量由各个多核苷酸编码的每种多肽的活性或性质。本发明还涉及通过这些方法获得的编码感兴趣的多肽的分离的多核苷酸，包括分离的多核苷酸的核酸构建体，表达载体和重组宿主细胞，以及由分离的多核苷酸编码的多肽的制备方法。

7. 发明申请

US20060172379A1 Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权
公开(公告)号：US20060172379A1
公开(公告)日：2006-08-03
申请号：US11140150
申请日：2005-05-26
申请人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
发明人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
IPC分类号： C12P21/06 , C12N1/16 , C12N15/74
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

8. 发明申请

US20080299613A1 Compositions for degrading cellulosic material 有权
标题翻译：降解纤维素材料的组合物
公开(公告)号：US20080299613A1
公开(公告)日：2008-12-04
申请号：US12130838
申请日：2008-05-30
申请人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
发明人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
IPC分类号： C12P21/04 , C12N1/14 , C12N9/42 , C12P1/00
CPC分类号： C12N9/2437 , C07K14/37 , C12N9/2445 , C12P7/10 , C12P19/14 , C12P19/16 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.
摘要翻译：本发明涉及用于降解或转化含纤维素材料的纤维素分解组合物以及制备和使用该组合物的方法。

9. 发明授权

US09969993B2 Filamentous fungal host cells and methods of recombinantly producing proteins 有权
公开(公告)号：US09969993B2
公开(公告)日：2018-05-15
申请号：US12130838
申请日：2008-05-30
申请人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
发明人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
IPC分类号： A61K38/00 , C12N9/42 , C07K14/37 , C12P7/10 , C12P19/14 , C12P19/16
CPC分类号： C12N9/2437 , C07K14/37 , C12N9/2445 , C12P7/10 , C12P19/14 , C12P19/16 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.

10. 发明申请

US20060019301A1 Methods of producing mutant polynucleotides 审中-公开
标题翻译：产生突变体多核苷酸的方法
公开(公告)号：US20060019301A1
公开(公告)日：2006-01-26
申请号：US11186641
申请日：2005-07-20
申请人： Peter Hansen , Mads Bjoernvad , Joel Cherry , Aubrey Jones , Amanda Fischer
发明人： Peter Hansen , Mads Bjoernvad , Joel Cherry , Aubrey Jones , Amanda Fischer
IPC分类号： C40B40/10 , C40B40/08 , C12Q1/68 , C07H21/04
CPC分类号： C40B50/06 , C12N15/102 , C12N15/1082 , C40B40/08
摘要： The present invention relates to methods of producing mutants of a polynucleotide and to mutant polynucleotides and artificial variants encoded by the mutant polynucleotides.
摘要翻译：本发明涉及产生多核苷酸的突变体的方法和由突变体多核苷酸编码的突变体多核苷酸和人造变体的方法。

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