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    • 1. 发明授权
    • Genes for synthesis of FR-008 polyketides
    • 用于合成FR-008聚酮化合物的基因
    • US07595156B2
    • 2009-09-29
    • US10819386
    • 2004-04-06
    • Sang Yup LeeZixin DengShi ChenKi Jun JeongXiufen Zhou
    • Sang Yup LeeZixin DengShi ChenKi Jun JeongXiufen Zhou
    • C12Q1/68C07H21/04
    • C12P19/62C07H21/04C07K14/36C12N15/52C12P17/08
    • The present invention relates to the base sequence of whole genes involved in the biosynthesis of FR-008 polyketides derived from Streptomyces sp. FR-008. This base sequence comprises genes coding for ketosynthase (KS), acyl transferase (AT), acyl carrier protein (ACP), ketoreductase (KR), dehydratase (DH) and enoyl reductase (ER) domains, and genes coding for modifier enzymes, such as ABC transporter, cytochrome P450 monooxygenase, ferredoxin, thioesterase, sugar synthetic protein, FAD-dependent monooxygenase, 4-amino-4-deoxychorismate (ADC) synthase and ADC lyase. The gene base sequence according to the present invention can be used to increase the productivity of the existing FR-008 polyketides or produce new FR-008 polyketides, through modification of its parts.
    • 本发明涉及参与由链霉菌属(Streptomyces sp。)生产的FR-008聚酮化合物的生物合成的全基因的碱基序列。 FR-008。 该碱基序列包括编码酮解酶(KS),酰基转移酶(AT),酰基载体蛋白(ACP),酮还原酶(KR),脱水酶(DH)和烯酰还原酶(ER)结构域的基因,以及编码修饰酶的基因, 作为ABC转运蛋白,细胞色素P450单加氧酶,铁氧还蛋白,硫酯酶,糖合成蛋白,FAD依赖性单加氧酶,4-氨基-4-脱氧激酶(ADC)合酶和ADC裂解酶。 根据本发明的基因碱基序列可用于通过改变其部分来提高现有FR-008聚酮化合物的生产率或生产新的FR-008聚酮化合物。