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1. 发明授权

US07803587B2 Method for developing culture medium using genome information and in silico analysis 有权
标题翻译：使用基因组信息和计算机分析开发培养基的方法
公开(公告)号：US07803587B2
公开(公告)日：2010-09-28
申请号：US11743668
申请日：2007-05-02
申请人： Sang Yup Lee , Ho Nam Chang , Hyohak Song , Tae Yong Kim , Bo-Kyung Choi
发明人： Sang Yup Lee , Ho Nam Chang , Hyohak Song , Tae Yong Kim , Bo-Kyung Choi
IPC分类号： C12P7/44 , C12N1/20 , C12Q1/00 , C12Q1/68 , G06G7/48
CPC分类号： C12N1/20 , C12N5/0018 , C12N2500/32 , C12N2500/38 , C12N2500/40 , C12P7/46 , G06F19/12
摘要： A method for developing a culture medium using genome information and in silico analysis. In one implementation, the method involves developing a minimal synthetic medium, including the steps of constructing a metabolic network using genome information of prokaryotic cell or eukaryotic cell, selecting components of the minimal synthetic medium removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation, and determining a final culture medium by actual culture.
摘要翻译：使用基因组信息和计算机分析开发培养基的方法。在一个实施方案中，该方法包括开发最小的合成培养基，包括使用原核细胞或真核细胞的基因组信息构建代谢网络的步骤，从构建的代谢网络中选择去除外部代谢物中的任何一种的最小合成培养基的组分，以及使用计算机模拟进行代谢通量分析，并通过实际培养确定最终培养基。

2. 发明授权

US08765446B2 Recombinant mutant microorganisms having increased ability to produce alcohols and method of producing alcohols using the same 有权
标题翻译：具有增加生产醇的能力的重组突变体微生物和使用其制备醇的方法
公开(公告)号：US08765446B2
公开(公告)日：2014-07-01
申请号：US13497736
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
发明人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
IPC分类号： C12N1/20 , C12N15/74 , C12N9/02 , C12N15/00 , C12P7/16 , C12Q1/26 , C07H21/04
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物。由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

3. 发明授权

US09217138B2 Engineered microorganism producing homo-succinic acid and method for preparing succinic acid using the same 有权
标题翻译：产生同型琥珀酸的工程微生物及使用其制备琥珀酸的方法
公开(公告)号：US09217138B2
公开(公告)日：2015-12-22
申请号：US12359322
申请日：2009-01-25
申请人： Sang Yup Lee , Sung Won Lim , Hyohak Song
发明人： Sang Yup Lee , Sung Won Lim , Hyohak Song
IPC分类号： C12P7/46 , C12N9/12 , C12N1/20 , C12N15/90 , C12N9/04 , C12N9/10
CPC分类号： C12P7/46 , C12N1/20 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N15/902 , C12Y203/01054 , Y02P20/52
摘要： The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.
摘要翻译：本发明涉及一种突变体微生物，其选自人嗜血杆菌属（Mannheimia），放线杆菌属（Actinobacillus）和放线虫属（Anaerobiospirillum）属，产生同型琥珀酸，以及使用其制备均琥珀酸的方法，更具体地说，涉及突变体通过破坏编码乳酸脱氢酶（ldhA）的基因，编码磷酸转乙酰酶（pta）的基因和编码乙酸激酶（ackA）的基因而获得的在厌氧条件下产生很少或不存在其它有机酸的微生物，产生高浓度的琥珀酸），而不破坏编码丙酮酸甲酸裂合酶（pfl）的基因，以及使用其生产琥珀酸的方法。与生产琥珀酸的现有菌株相比，本发明的突变微生物具有生长速度快和琥珀酸生产率少的特性，同时产生很少或不含有机酸。因此，本发明的突变微生物可用于生产用于工业用途的琥珀酸。

4. 发明申请

US20110269183A1 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
标题翻译：具有使用蔗糖作为碳源的能力的重组微生物
公开(公告)号：US20110269183A1
公开(公告)日：2011-11-03
申请号：US12808533
申请日：2008-12-18
申请人： Sang Yup Lee , Jeong Wook Lee , Hyohak Song , Ji Mahn Kim , Sol Choi , Jin Hwan Park
发明人： Sang Yup Lee , Jeong Wook Lee , Hyohak Song , Ji Mahn Kim , Sol Choi , Jin Hwan Park
IPC分类号： C12P21/00 , C12N9/26 , C12N15/63 , C12N15/56 , C12N1/19 , C12N1/21 , C12P1/04 , C12P1/02 , C12N9/12 , C12N1/15
CPC分类号： C12N15/70 , C12N1/20 , C12N9/1205 , C12N9/16 , C12N9/2408 , C12P1/04 , C12P7/065 , C12P7/40 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/08 , C12Y207/01004 , C12Y207/01069 , C12Y302/01026 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.
摘要翻译：本发明涉及能够代谢蔗糖的重组微生物，更具体地说，涉及能够代谢蔗糖的重组微生物，其中导入编码蔗糖磷酸转移酶和/或编码蔗糖-6-磷酸水解酶的基因的基因或重组微生物能够代谢其中引入编码“呋喃果糖苷酶”的基因的蔗糖。根据本发明，提供了能够使用廉价的蔗糖作为碳源代替昂贵的葡萄糖的重组微生物。此外，在不能使用蔗糖作为碳源的微生物的培养方法中，蔗糖可以替代包括葡萄糖在内的其它碳源。

5. 发明授权

US08877482B2 Homo-succinic acid producing microorganism variant and process for preparing succinic acid using the same 有权
标题翻译：产琥珀酸的微生物变异体及使用其制备琥珀酸的方法
公开(公告)号：US08877482B2
公开(公告)日：2014-11-04
申请号：US12668868
申请日：2008-01-15
申请人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
发明人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
IPC分类号： C12N1/20 , C12N15/00 , C12N15/74 , C12P7/46 , C12N9/04 , C12N9/12
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏乳酸脱氢酶编码基因（idhA）和一种乙酸激酶编码基因（ackA），以及一种高浓度均聚琥珀酸的制备方法，其包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

6. 发明申请

US20120301936A1 RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME 有权
标题翻译：具有增加生产酒精的能力的重组突变体微生物和使用该醇的生产醇的方法
公开(公告)号：US20120301936A1
公开(公告)日：2012-11-29
申请号：US13497736
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
发明人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
IPC分类号： C12P7/16 , C12N1/21 , C12N15/74
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物。由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

7. 发明申请

US20100159544A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 有权
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：US20100159544A1
公开(公告)日：2010-06-24
申请号：US12668868
申请日：2008-01-15
申请人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
发明人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
IPC分类号： C12P7/46 , C12N1/00 , C12N1/21 , C12N15/00
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏乳酸脱氢酶编码基因（idhA）和一种乙酸激酶编码基因（ackA），以及一种高浓度均聚琥珀酸的制备方法，其包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

8. 发明授权

US07241594B2 Gene encoding formate dehydrogenases D & E and method for preparing succinic acid using the same 有权
标题翻译：编码甲酸脱氢酶D＆E的基因及使用其制备琥珀酸的方法
公开(公告)号：US07241594B2
公开(公告)日：2007-07-10
申请号：US11228945
申请日：2005-09-16
申请人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Sung Won Lim
发明人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Sung Won Lim
IPC分类号： C12P1/00 , C12P21/04
CPC分类号： C12P7/46 , C12N9/0008
摘要： Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.
摘要翻译：编码甲酸脱氢酶D＆E的核苷酸序列和使用其编码琥珀酸的方法，更具体地说，将甲酸转化为二氧化碳和氢的甲酸脱氢酶D＆E，编码甲酸脱氢酶D＆E的fdhD和fdhE核苷酸序列，重组载体含有核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

9. 发明授权

US07470770B2 Gene encoding malic enzyme and method for preparing succinic acid using the same 有权
标题翻译：基因编码苹果酸酶及使用其制备琥珀酸的方法
公开(公告)号：US07470770B2
公开(公告)日：2008-12-30
申请号：US11229368
申请日：2005-09-16
申请人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Jeong Wook Lee
发明人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Jeong Wook Lee
IPC分类号： C07K14/00 , C12N15/11 , C12N15/63 , C12N1/20 , C12P13/20
CPC分类号： C12N9/0006 , C12P7/46
摘要： A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.
摘要翻译：编码苹果酸的核苷酸序列和使用该琥珀酸的琥珀酸的制备方法，特别是编码具有将丙酮酸或丙酮酸转化成苹果酸或苹果酸的活性的苹果酸B的maeB核苷酸序列，反之亦然，含有该基因的重组载体，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

10. 发明授权

US07572615B2 Gene encoding formate dehydrogenases D and E and method for preparing succinic acid using the same 有权
标题翻译：编码甲酸脱氢酶D和E的基因及使用其制备琥珀酸的方法
公开(公告)号：US07572615B2
公开(公告)日：2009-08-11
申请号：US11738463
申请日：2007-04-20
申请人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Sung Won Lim
发明人： Sang Yup Lee , Hyohak Song , Yu Sin Jang , Sung Won Lim
IPC分类号： C12N9/00 , C12P21/00
CPC分类号： C12P7/46 , C12N9/0008
摘要： Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences, microorganisms transformed with the recombinant vectors, and a method for preparing succinic acid using the transformed microorganism.
摘要翻译：编码甲酸脱氢酶D＆E的核苷酸序列和使用其编码琥珀酸的方法，更具体地说，将甲酸转化为二氧化碳和氢的甲酸脱氢酶D＆E，编码甲酸脱氢酶D＆E的fdhD和fdhE核苷酸序列，重组载体含有核苷酸序列，用重组载体转化的微生物，以及使用转化的微生物制备琥珀酸的方法。

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