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1. 发明授权

US06472188B1 Method for producing hydroxycarboxylic acids by auto-degradation of polyhydroxyalkanoates 失效
标题翻译：通过聚羟基链烷酸酯的自分解生产羟基羧酸的方法
公开(公告)号：US06472188B1
公开(公告)日：2002-10-29
申请号：US09554948
申请日：2000-05-22
申请人： Sang Yup Lee , Fulai Wang , Young Lee
发明人： Sang Yup Lee , Fulai Wang , Young Lee
IPC分类号： C12P740
CPC分类号： C12P7/42 , Y10S435/822
摘要： The present invention provides a method for producing. optically active hydroxycarboxylic acid monomers by auto-degradation of polyhydroxyalkanoates (PHAs). In particular, the present invention provides a method for producing hydroxycarboxylic acid monomers (mostly optically active in (R)-(−)-configuration) comprising the steps of: (a) synthesizing and accumulating PHAs by culturing various microorganisms; and (b) preparing optically active hydroxycarboxylic acids which are monomers of PHAs, by auto-degradation of PHAs by keeping the cultured microorganism in a degradation solution such as water, salt solution, mixture of water and organic solvents, and buffer solution. The present invention also provides a method for the separation of the prepared (R)-(−)-hydroxycarboxylic acids using liquid chromatography (LC) or high performance liquid chromatography (HPLC), and also provides further purification method by removing impurities from the purely separated (R)-(−)-hydroxycarboxylic acids by organic solvent extraction and powder-making process of drying the purified hydroxycarboxylic acids. The present method is economical since hydroxycarboxylic acids can be efficiently produced with high purity and yield by simple process. Also, the present method is environmentally friendly since organic solvents, which are required in large amounts in conventional methods, are used only in minimal amounts in the present invention.
摘要翻译：本发明提供一种制造方法。光学活性羟基羧酸单体通过聚羟基链烷酸酯（PHA）的自分解。特别地，本发明提供了一种生产羟基羧酸单体（主要是（R） - （ - ） - 构型的光学活性）的方法，包括以下步骤：（a）通过培养各种微生物合成和积累PHA; 和（b）通过将培养的微生物保持在降解溶液如水，盐溶液，水和有机溶剂的混合物以及缓冲溶液中，通过PHA的自动降解来制备作为PHA单体的光学活性羟基羧酸。本发明还提供了使用液相色谱（LC）或高效液相色谱（HPLC）分离制备的（R） - （ - ） - 羟基羧酸的方法，并且还提供了从纯的方法除去杂质的进一步纯化方法通过有机溶剂萃取和干燥纯化的羟基羧酸的粉末制备方法分离（R） - （ - ） - 羟基羧酸。本方法是经济的，因为通过简单的方法可以高纯度和高产率地有效地生产羟基羧酸。此外，本方法是环境友好的，因为在本发明中仅以最少量使用常规方法中大量需要的有机溶剂。

2. 发明申请

US20130017588A1 RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
标题翻译：具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法
公开(公告)号：US20130017588A1
公开(公告)日：2013-01-17
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N15/01
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

3. 发明授权

US09096872B2 Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same 有权
标题翻译：具有提高丁醇生产能力的重组微生物和使用其生产丁醇的方法
公开(公告)号：US09096872B2
公开(公告)日：2015-08-04
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N9/04 , C12N9/10
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

4. 发明申请

US20110027845A1 ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME 审中-公开
标题翻译：生产微生物的增强的乙醇和丁醇和使用它们制备乙醇和丁醇的方法
公开(公告)号：US20110027845A1
公开(公告)日：2011-02-03
申请号：US12809323
申请日：2008-12-22
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Kwang Seop Jung , Jae Hyun Kim
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Kwang Seop Jung , Jae Hyun Kim
IPC分类号： C12P7/16 , C12N1/00 , C12N1/21 , C12P7/06
CPC分类号： C12P7/065 , C12N9/0006 , C12N9/13 , C12P7/16 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.
摘要翻译：本发明涉及具有提高乙醇和丁醇生产能力的重组微生物，以及使用该重组微生物制备乙醇和丁醇的方法，更具体地说，涉及生产乙醇和丁醇的能力增强的重组微生物，其中基因引入编码CoA转移酶和编码醇/醛脱氢酶的基因，以及使用其制备乙醇和丁醇的方法。根据本发明的通过操纵微生物代谢途径获得的重组微生物能够仅生产丁醇和乙醇而不产生任何副产物，因此可用作产生工业溶剂和运输燃料的微生物。

5. 发明授权

US09145583B2 Multi-spot metal-capped nanostructure array nucleic acid chip for diagnosis of corneal dystrophy and preparation method thereof 有权
标题翻译：用于诊断角膜营养不良的多点金属封端纳米结构阵列核酸芯片及其制备方法
公开(公告)号：US09145583B2
公开(公告)日：2015-09-29
申请号：US13391167
申请日：2010-08-18
申请人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
发明人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
IPC分类号： C12Q1/68 , C12M1/36 , B82Y30/00
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.
摘要翻译：一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

6. 发明授权

US08895276B2 Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same 有权
标题翻译：具有烃生产能力的微生物变体及使用其生产烃的方法
公开(公告)号：US08895276B2
公开(公告)日：2014-11-25
申请号：US13702920
申请日：2011-06-10
申请人： Sang Yup Lee , Yong Jun Choi
发明人： Sang Yup Lee , Yong Jun Choi
IPC分类号： C12P5/00 , C12N9/02 , C12N9/18 , C12N9/88 , C12N9/00 , C12P5/02 , C12N15/52 , C12N15/70 , C07K14/245
CPC分类号： C12P5/00 , C07K14/245 , C12N9/001 , C12N9/18 , C12N9/88 , C12N9/93 , C12N15/52 , C12N15/70 , C12P5/005 , C12P5/02 , C12P5/026
摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.
摘要翻译：本发明涉及具有生产碳氢化合物（包括烷烃）的能力的微生物变异体，以及使用该方法生产烃类的方法，包括使用烷烃的烷烃，更具体地说，涉及通过引入编码转化脂肪酶的基因而获得的微生物变体对游离脂肪酸的酰基-Acp，编码将游离脂肪酸转化为脂肪酰辅酶A的酶的基因，编码将脂肪酰基-CoA转化为脂肪醛的酶的基因和编码将脂肪醛转化为烷烃的酶的基因转化为微生物改进以适于生产包括烷烃在内的烃类，以及使用其制备包括烷烃在内的烃类的方法。本发明的微生物变体具有很高的潜力用于通过附加的代谢通量工程来改善菌株，因此可用于包括烷烃在内的烃的工业生产。

7. 发明授权

US08877482B2 Homo-succinic acid producing microorganism variant and process for preparing succinic acid using the same 有权
标题翻译：产琥珀酸的微生物变异体及使用其制备琥珀酸的方法
公开(公告)号：US08877482B2
公开(公告)日：2014-11-04
申请号：US12668868
申请日：2008-01-15
申请人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
发明人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
IPC分类号： C12N1/20 , C12N15/00 , C12N15/74 , C12P7/46 , C12N9/04 , C12N9/12
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏乳酸脱氢酶编码基因（idhA）和一种乙酸激酶编码基因（ackA），以及一种高浓度均聚琥珀酸的制备方法，其包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

8. 发明申请

US20140243222A1 MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME 有权
标题翻译：多点金属填充纳米结构阵列核苷酸芯片，用于诊断角膜晶状体及其制备方法
公开(公告)号：US20140243222A1
公开(公告)日：2014-08-28
申请号：US13391167
申请日：2010-08-18
申请人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
发明人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.
摘要翻译：一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

9. 发明申请

US20120301936A1 RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME 有权
标题翻译：具有增加生产酒精的能力的重组突变体微生物和使用该醇的生产醇的方法
公开(公告)号：US20120301936A1
公开(公告)日：2012-11-29
申请号：US13497736
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
发明人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
IPC分类号： C12P7/16 , C12N1/21 , C12N15/74
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物。由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

10. 发明授权

US08067341B2 Method for fabricating a biochip using the high density carbon nanotube film or pattern 有权
标题翻译：使用高密度碳纳米管膜或图案制造生物芯片的方法
公开(公告)号：US08067341B2
公开(公告)日：2011-11-29
申请号：US10805044
申请日：2004-03-19
申请人： Hee Tae Jung , Sang Yup Lee , Dae Hwan Jung , Byung Hun Kim , Young Koan Ko
发明人： Hee Tae Jung , Sang Yup Lee , Dae Hwan Jung , Byung Hun Kim , Young Koan Ko
IPC分类号： C40B60/00
CPC分类号： G01N33/54353 , B82Y30/00 , G01N33/551 , Y10S977/742
摘要： Carbon nanotube (CNT) films, patterns and biochips and methods of making the same are provided. Such a biochip comprises a bio-receptor attached by means of an exposed chemical functional group on a surface of a high density CNT film or pattern produced by repeated lamination of CNTs on a substrate with exposed amine groups. Various types of CNT-biochips may be fabricated by bonding of bio-receptors to a CNT pattern (or film) containing exposed carboxyl groups or modified by various chemical functional groups. Further, the CNT-biochip may be used to measure an electrical or electrochemical signal using both conductor and semiconductor properties of the CNT, thereby not needing labeling. Upon fluorescent measurement of DNA hybridization using such a CNT-DNA chip it is possible to show more distinct signals useful for genotyping, mutation detection, pathogen identification and the like.
摘要翻译：提供了碳纳米管（CNT）膜，图案和生物芯片及其制造方法。这样的生物芯片包括通过暴露的化学官能团在高密度CNT膜的表面上附着的生物受体或通过在具有暴露的胺基的基底上重复层压CNT而产生的图案。可以通过将生物受体结合到包含暴露的羧基或被各种化学官能团修饰的CNT图案（或膜）来制造各种类型的CNT-生物芯片。此外，CNT-生物芯片可以用于使用CNT的导体和半导体性质来测量电或电化学信号，从而不需要标记。在使用这种CNT-DNA芯片进行DNA杂交的荧光测量时，可能显示更有用于基因分型，突变检测，病原体鉴定等的不同信号。

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