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1. 发明授权

US06444460B1 Microbial production of actinol 有权
标题翻译：微生物生成的肌动蛋白
公开(公告)号：US06444460B1
公开(公告)日：2002-09-03
申请号：US09375129
申请日：1999-08-16
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12P726
CPC分类号： C12R1/06 , C12P7/26 , C12P41/002 , C12R1/01 , C12R1/15
摘要： A process for making (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone by contacting (6R)-2,2,6-trimethylcyclohexanedione with a microorganism which is selected from microorganisms of the genera Cellulomonas, Corynebacterium, Planococcus and Arthrobacter and which is capable of the selective asymmetric reduction of (6R)-2,2,6-trimethylcyclohexanedione to (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone, and recovering the resulting (4R, 6R)-4-hydroxy-2,2,6-trimethylcyclohexanone from the reaction mixture. The selective asymmetric reduction can be effected in the presence of a co-factor, such as, nicotinamide adenine dinucleotide (NAD), nicotinamide adenine dinucleotide phosphate (NADP), or said co-factor with glucose and glucose dehydrogenase (GDH), and/or in the presence of a surfactant. The product is useful for the synthesis of carotenoids, such as, zeaxanthin.
摘要翻译：通过使（6R）-2,2,6-三甲基环己二酮与微生物接触，制备（4R，6R）-4-羟基-2,2,6-三甲基环己酮的方法，所述微生物选自Cellulomonas，Corynebacterium，Planococcus 并且能够（6R）-2,2,6-三甲基环己二酮选择性不对称还原成（4R，6R）-4-羟基-2,2,6-三甲基环己酮，并回收得到的（4R，6R ）-4-羟基-2,2,6-三甲基环己酮。选择性不对称还原可以在辅因子存在下进行，例如烟酰胺腺嘌呤二核苷酸（NAD），烟酰胺腺嘌呤二核苷酸磷酸（NADP）或与葡萄糖和葡萄糖脱氢酶（GDH）的所述辅因子，和/ 或在表面活性剂存在下。该产品可用于合成类胡萝卜素，如玉米黄质。

2. 发明授权

US06218156B1 Gene encoding carbonyl reductase, and methods for its use 有权
标题翻译：基因编码羰基还原酶及其使用方法
公开(公告)号：US06218156B1
公开(公告)日：2001-04-17
申请号：US09367012
申请日：1999-11-24
申请人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
发明人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
IPC分类号： C12P762
CPC分类号： C12P7/62 , C12N9/0006 , C12P41/002
摘要： An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.
摘要翻译：具有不对称还原羰基化合物以产生光学活性醇的羰基还原活性的酶，编码该酶的DNA，具有该DNA的质粒，作为用该质粒转化的细胞的转化体的转化体和光学活性物质的制备方法提供使用酶和/或转化细胞的醇。

3. 发明授权

US06534297B2 Recombinantly producing levodione reductase 失效
公开(公告)号：US06534297B2
公开(公告)日：2003-03-18
申请号：US09773748
申请日：2001-01-31
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12N902
CPC分类号： C12N9/0008 , C12P7/26
摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.

4. 发明授权

US06448052B2 Carbonyl reductase enzyme and methods for its use 有权
标题翻译：羰基还原酶及其使用方法
公开(公告)号：US06448052B2
公开(公告)日：2002-09-10
申请号：US09777157
申请日：2001-02-05
申请人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
发明人： Yoshihiko Yasohara , Noriyuki Kizaki , Junzo Hasegawa , Masaru Wada , Sakayu Shimizu , Michihiko Kataoka , Kazuhiko Yamamoto , Hiroshi Kawabata , Keiko Kita
IPC分类号： C12P762
CPC分类号： C12P7/62 , C12N9/0006 , C12P41/002
摘要： An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.
摘要翻译：具有不对称还原羰基化合物以产生光学活性醇的羰基还原活性的酶，编码该酶的DNA，具有该DNA的质粒，作为用该质粒转化的细胞的转化体的转化体和光学活性物质的制备方法提供使用酶和/或转化细胞的醇。

5. 发明授权

US06673583B2 Recombinantly producing levodione reductase 失效
标题翻译：重组产左旋酮还原酶
公开(公告)号：US06673583B2
公开(公告)日：2004-01-06
申请号：US10327726
申请日：2002-12-23
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12P724
CPC分类号： C12N9/0008 , C12P7/26
摘要： The present invention is directed to genetic material useful for the preparation of actinol, such as an isolated DNA including a nucleotide sequence coding for an enzyme having levodione reductase activity, a polypeptide encoded by such a DNA, recombinant organisms, and the like. These genetic materials may originate from Corynebacterium, Cellulomonas, Planococcus, Arthrobacter, and the like. The present invention also provides a process for the production of actinol.
摘要翻译：本发明涉及可用于制备锕系元素的遗传物质，例如分离的DNA，包括编码具有左旋体还原酶活性的酶的核苷酸序列，由这种DNA编码的多肽，重组生物等。这些遗传物质可以来自棒状杆菌属，丝杆菌属，球菌属，节杆菌属等。本发明还提供了制备肌动蛋白的方法。

6. 发明申请

US20050244941A1 Process for producing levodione 审中-公开
标题翻译：生产左氧氟沙星的方法
公开(公告)号：US20050244941A1
公开(公告)日：2005-11-03
申请号：US10505314
申请日：2003-02-15
申请人： Sakayu Shimizu , Masaru Wada
发明人： Sakayu Shimizu , Masaru Wada
IPC分类号： C12N15/09 , C12N9/02 , C12N15/53 , C12P7/26 , C12N1/18 , C12N15/74
CPC分类号： C12P7/26
摘要： An enone reductase characterized by a molecular mass of 61,300±5,000 Da; NADPH and NADH as co-factor; a temperature optimum of 55-60° C. at pH 7.4; a pH optimum of 4.5-8.5 and a substrate specificity on α,β-unsaturated ketones, especially derived from a yeast and a process for the preparation of levodione from ketoisophorane.
摘要翻译：特征在于分子量为61,300±5,000Da的烯酮还原酶; NADPH和NADH作为辅因子; 温度最适为55-60℃，pH 7.4; pH最适为4.5-8.5，对α，β-不饱和酮的底物特异性，特别是衍生自酵母，以及由酮二异戊酸制备左旋二酮的方法。

7. 发明授权

US06465228B1 Levodione reductase 有权
标题翻译：左旋去氧还原酶
公开(公告)号：US06465228B1
公开(公告)日：2002-10-15
申请号：US09495523
申请日：2000-01-31
申请人： Shigeru Nakamori , Sakayu Shimizu , Masaru Wada
发明人： Shigeru Nakamori , Sakayu Shimizu , Masaru Wada
IPC分类号： C12N902
CPC分类号： C12N9/0008 , C12P7/26 , C12P41/002
摘要： A levodione reductase having the following physical properties is provided: molecular weight: from about 142,000 to about 155,000±10,000 (consisting of four homologous subunits having a molecular weight of 36,000±5,000); co-factor: nicotinamide adenine dinucleotide (AND/NADH); substrate specificity: active on levodione; optimum temperature: about 15° C. to about 20° C. at pH 7.0; optimum pH: 7.5; and activator: K+, Cs+, Rb+, Na+ and NH4+. The levodione reductase according to the present invention produces actinol, an important intermediate for the production of zeaxanthin, from levodione. This enzyme may be produced from a microorganism belonging to the genus Corynebacterium, preferably the microorganism Corynebacterium aquaticum AKU 611 (FERM BP-6448) or a functional equivalent, subculture, mutant or variant thereof. Also provided is a process for producing the levodione reductase that includes cultivating a microorganism of the genus Corynebacterium in an aqueous medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the levodione reductase from the cell-free extract. A process for producing actinol from levodione is also provided that includes contacting levodione with levodione reductase in the presence of the reduced form of nicotinamide adenine dinucleotide or a cell-free extract of the microorganism, and then isolating the resulting actinol from the reaction mixture.
摘要翻译：提供了具有以下物理性质的左旋二酮还原酶：分子量：约142,000至约155,000±10,000（由分子量为36,000±5,000的四个同源亚基组成）; 辅因子：烟酰胺腺嘌呤二核苷酸（AND / NADH）; 底物特异性：对左旋多巴有活性; 最适温度：约15℃至约20℃，pH 7.0; 最适pH：7.5; 和活化剂：K +，Cs +，Rb +，Na +和NH4 +。根据本发明的左氧多糖还原酶从左旋二酮生产作为玉米黄质生产的重要中间体的肌动蛋白。该酶可以由属于棒状杆菌属的微生物，优选微生物棒状杆菌属水杨酸AKU 611（FERM BP-6448）或其功能等同的传代培养，突变体或变体产生。还提供了生产左旋体还原酶的方法，其包括在需氧条件下在水性介质中培养棒状杆菌属的微生物，破坏微生物的细胞并从无细胞提取物中分离和纯化左旋体还原酶。还提供了用于从左旋二酮生产锕系元素的方法，包括在还原形式的烟酰胺腺嘌呤二核苷酸或微生物的无细胞提取物的存在下将左旋多糖与左旋二酮还原酶接触，然后从反应混合物中分离得到的锕系元素。

8. 发明授权

US08273562B2 Method for producing 4-hydroxy-L-isoleucine 有权
公开(公告)号：US08273562B2
公开(公告)日：2012-09-25
申请号：US13344110
申请日：2012-01-05
申请人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Vitaly Grigorievich Paraskevov, legal representative , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
发明人： Tomohiro Kodera , Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Yury Ivanovich Kozlov , Sakayu Shimizu , Jun Ogawa , Makoto Hibi
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12Q1/68 , C12P21/06 , C12P21/04 , C07H21/04
CPC分类号： C12N9/0069 , C12P13/06
摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

9. 发明授权

US07927843B2 Method of preparing 2-deoxyribose 5-phosphate 有权
标题翻译： 2-脱氧核糖5-磷酸的制备方法
公开(公告)号：US07927843B2
公开(公告)日：2011-04-19
申请号：US11878558
申请日：2007-07-25
申请人： Sakayu Shimizu , Jun Ogawa
发明人： Sakayu Shimizu , Jun Ogawa
IPC分类号： C12P19/02
CPC分类号： C12R1/22 , C12N9/88 , C12N9/90 , C12P19/02
摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.
摘要翻译：本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸盐的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法，微生物。本发明还公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸盐的微生物本身存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者源于微生物的酶。

10. 发明申请

US20080261281A1 Novel Carbonyl Reductase, Gene Thereof and Method of Using the Same 有权
标题翻译：新型羰基还原酶，其基因及其使用方法
公开(公告)号：US20080261281A1
公开(公告)日：2008-10-23
申请号：US11659481
申请日：2005-08-01
申请人： Noriyuki Kizaki , Miho Yano , Masahiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
发明人： Noriyuki Kizaki , Miho Yano , Masahiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12N9/14 , C12N15/52 , C12N1/21 , C12P13/02
CPC分类号： C12P13/02 , C12N9/0006 , C12P41/002
摘要： The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant.Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
摘要翻译：本发明涉及具有不对称还原（3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁酮的活性以产生（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4- 从属于Ogataea的微生物分离的丁氧基羰基氨基-4-苯基-2-丁醇，编码多肽的DNA和产生多肽的转化体。本发明还涉及利用多肽或转化体制备（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇的方法。使用本发明的多肽或转化体，可以有效地制备光学活性醇如（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇等。

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