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1. 发明授权

US11123656B2 Separation/analysis method for mixture of oligonucleotides 有权
公开(公告)号：US11123656B2
公开(公告)日：2021-09-21
申请号：US16609938
申请日：2018-06-21
申请人： SHOWA DENKO K.K.
发明人： Junji Sasuga , Hirobumi Aoki , Yuzuru Kokido
IPC分类号： B01D15/32 , B01J20/285 , G01N30/34 , G01N30/50 , G01N30/72 , G01N30/74 , G01N30/88 , B01D15/16 , B01D15/42 , B01D15/20 , B01J20/32 , C07K1/20 , B01J20/28
摘要： The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.

2. 发明授权

US09434965B2 Production method for organic acid using CoA-transferase 有权
标题翻译：使用CoA转移酶的有机酸的生产方法
公开(公告)号：US09434965B2
公开(公告)日：2016-09-06
申请号：US14351497
申请日：2012-10-10
申请人： SHOWA DENKO K.K. , NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
发明人： Ken'ichiro Matsumoto , Seiichi Taguchi , Hirobumi Aoki
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.
摘要翻译：本发明涉及一种生产有机酸的方法，包括使用乙酰辅酶A作为底物生物合成有机酸-CoA，并在乙酸存在下使用CoA转移酶进行有机酸-CoA的CoA消除反应，其中通过用乙酰CoA作为底物和CoA转移酶基因培养具有用于合成有机酸-CoA的酶基因簇的转化微生物获得有机酸。

3. 发明申请

US20140234927A1 PRODUCTION METHOD FOR ORGANIC ACID USING COA-TRANSFERASE 有权
标题翻译：使用COA转移的有机酸的生产方法
公开(公告)号：US20140234927A1
公开(公告)日：2014-08-21
申请号：US14351497
申请日：2012-10-10
申请人： SHOWA DENKO K.K. , NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
发明人： Ken'ichiro Matsumoto , Seiichi Taguchi , Hirobumi Aoki
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.
摘要翻译：本发明涉及一种生产有机酸的方法，包括使用乙酰辅酶A作为底物生物合成有机酸-CoA，并在乙酸存在下使用CoA转移酶进行有机酸-CoA的CoA消除反应，其中通过用乙酰CoA作为底物和CoA转移酶基因培养具有用于合成有机酸-CoA的酶基因簇的转化微生物获得有机酸。

4. 发明授权

US09677096B2 Manufacturing method for 1,4-butanediol, microbe, and gene 有权
公开(公告)号：US09677096B2
公开(公告)日：2017-06-13
申请号：US14440116
申请日：2013-11-28
申请人： SHOWA DENKO K.K.
发明人： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
IPC分类号： C12P7/18 , C12N9/90 , C12N9/88 , C12N9/10 , C12N9/04 , C12N15/52
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/1029 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01035 , C12Y101/01036 , C12Y101/01157 , C12Y203/01009 , C12Y402/01017 , C12Y402/01055 , C12Y402/01119 , C12Y402/0112 , C12Y503/03003
摘要： A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.

5. 发明申请

US20150291985A1 METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE 审中-公开
标题翻译：制备1,4-丁二醇和微生物的方法
公开(公告)号：US20150291985A1
公开(公告)日：2015-10-15
申请号：US14439834
申请日：2013-09-20
申请人： SHOWA DENKO K.K.
发明人： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
IPC分类号： C12P7/18 , C12N9/04
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01036 , C12Y101/01157 , C12Y402/0112 , C12Y503/03003
摘要： A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.
摘要翻译：通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A的酶反应体系依次使用微生物和/或其培养物制备1,4-丁二醇的方法，其中3-羟基丁酰基 - CoA是光学活性物质，其中微生物包括（1）编码烯酰辅酶A水合酶的基因，（2）编码乙酰乙酰辅酶Aδ异构酶的基因，（3）编码4-羟基丁酰辅酶A脱水酶的基因，（4）编码酰基辅酶A还原酶的基因，其底物特异性具有与3-羟基丁酰辅酶A相反的光学选择性。

6. 发明申请

US20150232830A1 GENE, MICROBE, CONVERSION METHOD, AND MANUFACTURING METHOD 审中-公开
标题翻译：基因，MICROBE，转换方法和制造方法
公开(公告)号：US20150232830A1
公开(公告)日：2015-08-20
申请号：US14429459
申请日：2013-09-19
申请人： SHOWA DENKO K.K.
发明人： Hirobumi Aoki , Yuzuru Kokido , Tadashi Yoneda
IPC分类号： C12N9/88 , C12P7/18 , C12P19/32
CPC分类号： C12N9/88 , C12P7/16 , C12P7/18 , C12P19/32 , C12Y402/01017 , Y02E50/10
摘要： A gene is selected from the group consisting of a gene (a) that has a base sequence of sequence number 1, a gene (b) that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene (b) has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and a gene (c) that hybridizes with a gene that has a base sequence complimentary with a gene that has a base sequence of sequence number 1 on a stringent condition, wherein the selected gene in combination with a gene that codes enoyl-CoA hydratase is able to provide a microbe or a culture of the microbe with an ability to convert 3-hydroxybutyryl-CoA into 4-hydroxybutyryl-CoA or 4-hydroxybutyryl-CoA into 3-hydroxybutyryl-CoA.
摘要翻译：基因选自具有序列号1的碱基序列的基因（a），具有碱基序列的基因（b），使得一个或多个碱基被缺失，取代或加入到碱基中序列号1的序列，其中基因（b）具有相对于序列号1的碱基序列具有大于或等于90％的同一性的碱基序列，以及与具有与在严格条件下具有序列号1的碱基序列的基因互补的碱基序列，其中所选择的基因与编码烯酰辅酶A水合酶的基因组合能够提供微生物或微生物的培养物将3-羟基丁酰辅酶A转化为4-羟基丁酰辅酶A或4-羟基丁酰辅酶A的能力转化为3-羟基丁酰辅酶A。

7. 发明申请

US20150275236A1 METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE 审中-公开
标题翻译：制备1,4-丁二醇和微生物的方法
公开(公告)号：US20150275236A1
公开(公告)日：2015-10-01
申请号：US14438292
申请日：2013-09-13
申请人： SHOWA DENKO K.K.
发明人： Hirobumi Aoki , Yuzuru Kokido , Yoko Hashimoto , Tadashi Yoneda
IPC分类号： C12P7/18 , C12N9/02 , C12N9/90 , C12N9/04 , C12N9/88
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01035 , C12Y101/01036 , C12Y101/01157 , C12Y102/0101 , C12Y402/01017 , C12Y402/01055 , C12Y402/01119 , C12Y402/0112 , C12Y503/03003
摘要： A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.
摘要翻译：通过使用乙酰乙酰辅酶A还原酶和烯酰辅酶A水合酶的酶反应体系，通过乙酰乙酰辅酶A，3-羟基丁酰辅酶A和巴豆酰基-CoA的方法，使用微生物和/或其培养物制备1,4-丁二醇的方法， CoA，其中每个乙酰乙酰辅酶A还原酶和烯酰辅酶A水合酶对3-羟基丁酰辅酶A的立体异构体是特异性的。

8. 发明申请

US20150252390A1 MANUFACTURING METHOD FOR A BUTANEDIOL 审中-公开
标题翻译：丁二醇的制造方法
公开(公告)号：US20150252390A1
公开(公告)日：2015-09-10
申请号：US14429453
申请日：2013-08-20
申请人： SHOWA DENKO K.K.
发明人： Hirobumi Aoki , Yuzuru Kokido , Tadashi Yoneda
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N15/52 , C12Y101/01036 , C12Y203/01194
摘要： A method of manufacturing a butanediol includes a step of culturing a microbe that contains a gene that codes acetoacetyl-CoA synthase that catalyzes a reaction that irreversibly produces acetoacetyl-CoA from acetyl-CoA and malonyl-CoA, acetoacetyl-CoA reductase that catalyzes a reaction that produces 3-hydroxybutyryl-CoA from acetoacetyl-CoA, and an enzyme that catalyzes a reaction for producing a butanediol from 3-hydroxybutyryl-CoA.
摘要翻译：制备丁二醇的方法包括培养含有编码乙酰乙酰辅酶A合成酶的基因的微生物的步骤，其催化乙酰辅酶A和丙二酰辅酶A不可逆地产生乙酰乙酰辅酶A的反应，催化反应的乙酰乙酰辅酶A还原酶其从乙酰乙酰辅酶A产生3-羟基丁酰辅酶A，以及催化由3-羟基丁酰辅酶A生成丁二醇的反应的酶。

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