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1. 发明授权

US5866378A Process for the synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses 失效
标题翻译：用于合成核苷酸-6-脱氧-D-木糖-4-己糖苷的方法
公开(公告)号：US5866378A
公开(公告)日：1999-02-02
申请号：US731189
申请日：1996-10-07
申请人： Ruediger Marquardt , Brigitte Hoersch , Andreas Seiffert-Stoeriko , Andreas Stein , Astrid Zervosen , Lothar Elling , Maria Regina Kula , Stefan Verseck , Juergen Distler , Wolfgang Piepersberg
发明人： Ruediger Marquardt , Brigitte Hoersch , Andreas Seiffert-Stoeriko , Andreas Stein , Astrid Zervosen , Lothar Elling , Maria Regina Kula , Stefan Verseck , Juergen Distler , Wolfgang Piepersberg
IPC分类号： C07H19/04 , C07H19/10 , C07H19/20 , C12P19/30 , C12P19/02 , C12N11/18 , C12P19/04
CPC分类号： C12P19/30 , C07H19/04
摘要： The present invention relates to processes for the enzymatic synthesis of nucleotide-6-deoxy-D-xylo-4-hexuloses starting from a nucleoside monophosphate (NMP). These processes comprise simultaneous incubation of the following substances in a buffer solution:(a) substrates comprising a nucleoside monophosphate, phosphoenolpyruvate, adenosine triphosphate, and sucrose; and(b) enzymes comprising pyruvate kinase, nucleoside-monophosphate kinase, sucrose synthase and deoxythymidine-D-glucose 4,6dehydratase.
摘要翻译：本发明涉及从核苷单磷酸（NMP）开始的酶合成核苷酸-6-脱氧-D-木糖-4-己糖苷的方法。这些方法包括将以下物质同时孵育在缓冲溶液中：（a）包含核苷单磷酸，磷酸烯醇丙酮酸，三磷酸腺苷和蔗糖的底物; 和（b）包含丙酮酸激酶，核苷单磷酸激酶，蔗糖合成酶和脱氧胸苷-D-葡萄糖四氢叶酸酶的酶。

2. 发明授权

US5962275A Method for the enzymatic galactosylation of monosaccharides and oligosaccharides 失效
标题翻译：单糖和寡糖的酶促半乳糖基化方法
公开(公告)号：US5962275A
公开(公告)日：1999-10-05
申请号：US952197
申请日：1998-02-13
申请人： Brigitte Horsch , Andreas Seiffert-Storiko , Rudiger Marquardt , Astrid Zervosen , Lothar Elling , Maria Regina Kula
发明人： Brigitte Horsch , Andreas Seiffert-Storiko , Rudiger Marquardt , Astrid Zervosen , Lothar Elling , Maria Regina Kula
IPC分类号： C12P19/26 , C07H3/04 , C07H3/06 , C12P19/12 , C12P19/18 , C12P19/24 , C12P19/14
CPC分类号： C12P19/18 , C07H3/04 , C07H3/06 , C12P19/12 , C12P19/24
摘要： The invention relates to an improved process for enzymically galactosylating monosaccharides and oligosaccharides, with in-situ regeneration of the nucleotide sugar (or of the nucleoside diphosphate sugar), in the presence of sucrose synthase, .beta.-1-4-galactosyl transferase and uridine diphosphate-glucose 4'-epimerase, (UDP-glucose 4'-epimerase), in which process the uridine diphosphate-glucose 4'-epimerase is reactivated with a ketosugar derivative.
摘要翻译： PCT No.PCT / EP96 / 01828 Sec。 371日期1998年2月13日 102（e）1998年2月13日PCT PCT 1996年5月2日PCT公布。公开号WO96 / 35801 日期：1996年11月14日本发明涉及一种在蔗糖合成酶β-4-异构体存在下酶解半乳糖基化单糖和寡糖的改进方法，其中核苷酸糖（或核苷二磷酸糖）的原位再生，半乳糖基转移酶和尿苷二磷酸 - 葡萄糖4'-差向异构酶（UDP-葡萄糖4'-差向异构酶），其中尿苷二磷酸 - 葡萄糖4'-差向异构酶与酮糖衍生物再活化。

3. 发明授权

US07217550B2 Nucleotide-sugar-synthesizing enzymes from nonparasitic protists 失效
标题翻译：来自非寄生性原生生物的核苷酸 - 糖合成酶
公开(公告)号：US07217550B2
公开(公告)日：2007-05-15
申请号：US10436382
申请日：2003-05-12
申请人： Thomas Kiy , Lothar Elling , Maria Regina Kula
发明人： Thomas Kiy , Lothar Elling , Maria Regina Kula
IPC分类号： C12N9/12
CPC分类号： C12N9/1241 , Y10S435/947
摘要： The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.
摘要翻译：本发明涉及从非寄生型原生生物的核苷酸 - 糖合成酶（具有核苷酸转移酶或焦磷酸化酶活性的酶），其制备方法及其制备核苷酸 - 糖的用途。根据本发明的酶使得可能或大大简化了低成本前体在工业规模上的各种核糖 - 糖的酶制剂。借助所发现的酶可以制备例如GDP-岩藻糖，GDP-甘露糖，UDP-葡萄糖，UDP-葡糖胺，UDP-半乳糖，UDP-半乳糖胺，UDP-N-乙酰氨基葡萄糖和UDP-N- 乙酰半乳糖经济量。

4. 发明授权

US5750389A Purified saccharose-synthase, process for its production and its use 失效
标题翻译：纯化的蔗糖合成酶，其生产和使用的过程
公开(公告)号：US5750389A
公开(公告)日：1998-05-12
申请号：US367178
申请日：1995-01-06
申请人： Lothar Elling , Maria-Regina Kula
发明人： Lothar Elling , Maria-Regina Kula
IPC分类号： C07H19/10 , C07H19/20 , C12N9/10 , C12P19/30 , C12P19/38
CPC分类号： C07H19/10 , C07H19/20 , C12N9/1062 , C12P19/30 , C12P19/38
摘要： Nucleotide sugars, especially UDP, ADP, CDP or TDP saccharoses can be enzymatically obtained by the reaction of nucleoside diphosphates with di or trisaccharides with a saccharose synthase in which the virtual absence of nucleoside phosphatases (0.1% or less) can be ensured by special purification methods and sensitive detection. The purification of the raw extract, obtained preferably from rice grains, comprises especially the application of the ultra-filtered extract containing 50 mM KCl with a pH 8 on a sepharose Q column and a gradient elution out of the column at a pH 8 with 50 to 500 mM KCl.
摘要翻译： PCT No.PCT / DE93 / 00562 Sec。 371 1995年1月6日第 102（e）日期1995年1月6日PCT Filed June 26，1993 PCT Pub。第WO94 / 01540BC号公报日期1994年1月20日核苷酸糖，特别是UDP，ADP，CDP或TDP蔗糖可以通过核苷二磷酸与二糖或三糖与蔗糖合酶的反应获得酶，其中虚拟不存在核苷磷酸酶（0.1％或更少）可以通过特殊的净化方法和敏感检测来保证。优选从米粒获得的原始提取物的纯化特别包括在Sepharose Q柱上应用含有pH8的50mM KCl的超滤提取物，并在pH8下用柱梯度洗脱，用50 至500mM KCl。

5. 发明授权

US6143539A UDP-glucose pyro phosphorylase enzymes from nonparasitic protozoa 失效
标题翻译：来自非寄生虫原生动物的UDP-葡萄糖焦磷酸化酶
公开(公告)号：US6143539A
公开(公告)日：2000-11-07
申请号：US796697
申请日：1997-03-03
申请人： Thomas Kiy , Lothar Elling , Maria Regina Kula
发明人： Thomas Kiy , Lothar Elling , Maria Regina Kula
IPC分类号： C12N9/10 , C12N9/12 , C12P19/30 , C12R1/90
CPC分类号： C12N9/1241 , Y10S435/947
摘要： The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.
摘要翻译：本发明涉及从非寄生型原生生物的核苷酸 - 糖合成酶（具有核苷酸转移酶或焦磷酸化酶活性的酶），其制备方法及其制备核苷酸 - 糖的用途。根据本发明的酶使得可能或大大简化了低成本前体在工业规模上的各种核糖 - 糖的酶制剂。借助所发现的酶可以制备例如GDP-岩藻糖，GDP-甘露糖，UDP-葡萄糖，UDP-葡糖胺，UDP-半乳糖，UDP-半乳糖胺，UDP-N-乙酰氨基葡萄糖和UDP-N- 乙酰半乳糖经济量。

6. 发明授权

US06444454B1 Method for increasing the gene expression of saccharose synthase 失效
标题翻译：增加蔗糖合酶基因表达的方法
公开(公告)号：US06444454B1
公开(公告)日：2002-09-03
申请号：US09486291
申请日：2000-05-19
申请人： Wolf-Bernd Frommer , Henning Schrader , Lothar Elling
发明人： Wolf-Bernd Frommer , Henning Schrader , Lothar Elling
IPC分类号： C12N900
CPC分类号： C12N9/1062 , C12N15/81
摘要： The invention relates to a method for increasing the gene expression of saccharose synthase. To this end the invention provides for the expression of the saccharose synthase gene to be placed under the control of a proton-ATPase promoter. According to a preferred version of the invention gene expression of the saccharose synthase is also increased by increasing the number of copies of the saccharose synthase gene and of the proton-ATPase promoter.
摘要翻译：本发明涉及增加蔗糖合酶基因表达的方法。为此，本发明提供将蔗糖合成酶基因置于质子-ATP酶启动子控制下的表达。根据本发明的优选方案，蔗糖合成酶的基因表达也通过增加蔗糖合酶基因和质子-ATPase启动子的拷贝数而增加。

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