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1. 发明授权

US4868139A Aqueous cholesterol standard solution and process for its preparation 失效
标题翻译：水性胆固醇标准溶液及其制备方法
公开(公告)号：US4868139A
公开(公告)日：1989-09-19
申请号：US122019
申请日：1987-11-17
申请人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
发明人： Rolf Deeg , Gisela Dengler , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , Y10T436/104165 , Y10T436/108331
摘要： The present invention provides an aqueous cholesterol standard solution with a definite content of cholesterol, wherein it contains a detergent mixture of 10 to 90% of cholic acid and 90 to 10% of desoxycholic acid or of appropriate salts or derivatives of these acids.The present invention also provides a process for the preparation of this aqueous cholesterol standard solution, wherein a detergent mixture of cholic acid and desoxycholic acid or of appropriate salts and derivatives of these acids is dissolved in distilled water or in 0.9% aqueous sodium chloride solution, an appropriate preservation agent and/or a buffer effective in the pH range of from 7 to 9 optionally added thereto, and a definite, precisely defined amount of cholesterol is dissolved in the solution thus obtained, while stirring and warming to 40.degree. to 60.degree. C.
摘要翻译：本发明提供具有一定胆固醇含量的胆固醇水溶液，其中含有10-90％胆酸和90-10％脱氧胆酸或这些酸的适当盐或衍生物的洗涤剂混合物。本发明还提供了制备这种胆固醇水溶液标准溶液的方法，其中将胆酸和脱氧胆酸的洗涤剂混合物或这些酸的适当盐和衍生物溶解在蒸馏水中或在0.9％氯化钠水溶液中，适当的保存剂和/或在任意添加的pH范围为7〜9的有效缓冲液中，将一定精确定量的胆固醇溶于所得溶液中，同时搅拌并升温至40〜60℃ C。

2. 发明授权

US4816393A Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
标题翻译：核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途
公开(公告)号：US4816393A
公开(公告)日：1989-03-28
申请号：US704712
申请日：1985-02-25
申请人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
发明人： Joachim Siedel , Rolf Deeg , Albert Roder , Joachim Ziegenhorn , Hans Mollering , Helmgard Gauhl
IPC分类号： C12N9/14 , C12N9/86 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/13 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/32
CPC分类号： C12N9/86 , C12Q1/34 , G01N33/70 , G01N2333/986 , Y10S435/81
摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.
摘要翻译：本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解，测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

3. 发明授权

US4438204A Determination of glycosilated hemoglobin 失效
标题翻译：测定糖化血红蛋白
公开(公告)号：US4438204A
公开(公告)日：1984-03-20
申请号：US426510
申请日：1982-09-29
申请人： Rolf Deeg , Urban Schmitt , Joachim Ziegenhorn
发明人： Rolf Deeg , Urban Schmitt , Joachim Ziegenhorn
IPC分类号： G01N33/52 , G01N21/78 , G01N30/88 , G01N33/53 , G01N33/66 , G01N33/72
CPC分类号： G01N33/723 , Y10T436/107497
摘要： A process for the determination of glycosilated hemoglobin in a blood sample comprises liberating glycosilated and non-glycosilated hemoglobin from the erythrocytes by chemical or physical treatment; converting, if desired, the hemoglobin into methemoglobin, and thereafter differentiating the glycosilated and non-glycosilated hemoglobin portion using its reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated hemoglobin portion.The present invention also provides a reagent for the determination of glycosilated hemoglobin in a blood sample, as well as a reagent for the differentiation of glycosilated and non-glycosilated hemoglobin, comprising haptoglobin.
摘要翻译：用于测定血液样品中糖基化血红蛋白的方法包括通过化学或物理处理从红细胞释放糖基化和非糖基化血红蛋白; 如果需要，将血红蛋白转化成高铁血红蛋白，然后使用其与触珠蛋白的反应来分化糖基化和非糖基化血红蛋白部分，并测定糖基化和/或非糖基化血红蛋白部分。本发明还提供了用于测定血液样品中的糖基化血红蛋白的试剂，以及包含触珠蛋白的糖基化和非糖化血红蛋白的分化试剂。

4. 发明授权

US4503144A Method and composition for the determination of cholesterol 失效
标题翻译：测定胆固醇的方法和组成
公开(公告)号：US4503144A
公开(公告)日：1985-03-05
申请号：US328350
申请日：1981-12-07
申请人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
发明人： Rolf Deeg , Helmut Schlumberger , Joachim Ziegenhorn
IPC分类号： G01N33/50 , C12Q1/60 , C12Q1/26 , C12Q1/28 , C12Q1/44 , C12R1/465
CPC分类号： C12Q1/60 , Y10S435/805 , Y10S435/81 , Y10S435/886
摘要： The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol.The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.
摘要翻译：本发明提供了一种通过胆固醇氧化酶和任选的胆固醇酯酶通过测量所形成的过氧化氢或形成的胆碱酯的氧消耗来测定游离或结合形式的胆固醇的方法，其中进行测定动态地使用已经从链霉菌属的微生物获得的胆固醇氧化酶，并加入3,4-二氯苯酚。本发明还提供用于胆固醇的动力学测定的试剂，其包含胆固醇氧化酶和用于测定过氧化氢或胆石素和任选的胆固醇酯酶的系统，其中还含有3,4-二氯苯酚，所用的胆固醇氧化酶源自链霉菌属的微生物。

5. 发明授权

US4645739A Process and reagent for the determination of N-carbamoylsarcosine with the use of a new enzyme 失效
标题翻译：使用新酶测定N-氨基甲酰肌氨酸的方法和试剂
公开(公告)号：US4645739A
公开(公告)日：1987-02-24
申请号：US562072
申请日：1983-12-16
申请人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
发明人： Rolf Deeg , Albert Roder , Joachim Siedel , Helmgard Gauhl , Joachim Ziegenhorn
IPC分类号： C12N9/80 , C12Q1/34 , C12R1/01 , C12R1/06 , C12R1/265 , G01N33/70 , C12Q1/26 , C12Q1/00 , C12Q1/28
CPC分类号： G01N33/70 , C12N9/80 , C12Q1/34 , G01N2333/98 , Y10S435/81 , Y10S435/814 , Y10S435/815 , Y10S435/816 , Y10S435/822 , Y10S435/83 , Y10S435/859
摘要： The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined.The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.
摘要翻译：本发明提供了测定N-氨基甲酰基肌氨酸的方法，其中将含有N-氨基甲酰基肌氨酸的样品溶液与N-氨基甲酰基肌氨酸 - 酰胺水解酶反应，得到肌氨酸，然后测定。本发明还提供了N-氨基甲酰基肌氨酸 - 酰氨基水解酶，其获得方法和含有它的试剂。

6. 发明授权

US4695552A Process and reagent for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin 失效
标题翻译：在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法和试剂
公开(公告)号：US4695552A
公开(公告)日：1987-09-22
申请号：US601801
申请日：1984-04-19
申请人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
发明人： Urban Schmitt , Rolf Deeg , Joachim Ziegenhorn
IPC分类号： C12Q1/28 , G01N33/72
CPC分类号： C12Q1/28 , G01N33/725 , C12Q2326/30 , Y10T436/25125
摘要： The present invention provides a process for the determination of the hemoglobin-haptoglobin complex in the presence of free hemoglobin by utilization of the different peroxidate properties of free and of bound hemoglobin, wherein, for the selective inhibition of the peroxidase activity of the free hemoglobin, a detergent is added and the residual peroxidate activity of the reaction mixture is measured.The present invention also provides a reagent for carrying out this process wherein, besides the substances required for the determination of the peroxidase activity, it contains a detergent for the inhibition of the peroxidase activity of free hemoglobin.Furthermore, the present invention provides a process for determining the haptoglobin content of a sample, as well as a process for determining glycosilated hemoglobin in a sample.
摘要翻译：本发明提供了一种通过利用游离血红蛋白和结合血红蛋白的不同过氧化物特性在游离血红蛋白存在下测定血红蛋白 - 触珠蛋白复合物的方法，其中，对于选择性抑制游离血红蛋白的过氧化物酶活性，添加洗涤剂，并测量反应混合物的过氧化物的残留活性。本发明还提供了一种用于进行该方法的试剂，其中除了确定过氧化物酶活性所需的物质外，还含有用于抑制游离血红蛋白的过氧化物酶活性的洗涤剂。此外，本发明提供了测定样品的触珠蛋白含量的方法，以及测定样品中糖基化血红蛋白的方法。

7. 发明授权

US5288606A Reagent for specific determination of fructosamine 失效
标题翻译：具体测定果糖胺的试剂
公开(公告)号：US5288606A
公开(公告)日：1994-02-22
申请号：US911282
申请日：1992-07-09
申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
发明人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/30 , C12Q1/44 , C12Q1/54 , G01N33/66 , C12Q1/00 , A61K35/78 , A61K37/02 , C12P19/38
CPC分类号： C12Q1/26 , G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.
摘要翻译：本发明提供一种用于通过与适当的着色试剂反应并测量由此引起的颜色变化来特异性测定血液或血液中衍生血液的血清果糖胺含量的方法，其中在显色反应之前，非特异性还原在大约中性pH值下去除起泡和/或浊度的样品组分，随后将pH调节至10至12的值，并向其中加入着色剂。本发明还提供了用于特异性测定血液或血液中衍生血液中的果糖胺含量的试剂混合物，其中它包括用于除去非特异性还原作用和/或引起浊度的样品组分的试剂，具有pH值在10.5〜12.5范围内的缓冲液和用于检测果糖胺的显色试剂的缓冲试剂。

8. 发明授权

US5108913A Process for preparing a maltoheptaose derivative 失效
标题翻译：制备MALTOHEPTAOSE衍生物的方法
公开(公告)号：US5108913A
公开(公告)日：1992-04-28
申请号：US545092
申请日：1990-06-27
申请人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
发明人： Elli Rauscher , Ulrich Neumann , August W. Wahlefeld , Alexander Hagen , Wolfgang Gruber , Joachim Ziegenhorn , Eugen Schaich , Ulfert Deneke , Michal Gerhard , Gunter Weimann
IPC分类号： G01N33/50 , C07H15/04 , C07H15/203 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10 , Y10S435/805 , Y10S435/81
摘要： .alpha.-Amylase is determined by the enzymatic splitting of an .alpha.-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula ##STR1## wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by .alpha.-amylase, are also provided. A process for the preparation of a maltoheptaose will Bacillus macerans amylase, free of p-nitrophenyl-alpha-glucoside splitting activity is disclosed.

9. 发明授权

US4892815A Process and reagent for the specific determination of the cholesterol of the HDL fraction 失效
$Process and reagent for the specific determination of the cholesterol of the HDL fraction$
标题翻译：用于特异性测定HDL级分胆固醇的方法和试剂
公开(公告)号：US4892815A
公开(公告)日：1990-01-09
申请号：US107467
申请日：1987-10-06
申请人： Lorenz Kerscher , Brigitte Pautz , Gisela Trunk , Joachim Ziegenhorn
发明人： Lorenz Kerscher , Brigitte Pautz , Gisela Trunk , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92
CPC分类号： C12Q1/60 , G01N33/92 , G01N2800/044 , Y10S435/805 , Y10S435/962 , Y10S436/81 , Y10S436/824
摘要： The present invention provides a process for the specific determination of the cholesterol of the HDL fraction in the presence of the LDL fraction of serum lipoproteins. Pancreatic cholesterol esterase is used to liberate cholesterol, and the liberated cholesterol then reacts with cholesterol oxidase and oxygen to form hydrogen peroxide. The kinetics of either of hydrogen peroxide formation or oxygen consumption is measured within 2 to 15 minutes after the start of the reaction between cholesterol and the oxidase. The temperature is maintained within a range of 20.degree. C., during a predetermined time interval. Specific concentrations of reactants are maintained in the reaction solution, i.e., from 0.05 to 30 U/ml pancreatic cholesterol esterase; from 0.1 to 50 U/ml cholesterol oxidase; from 1.0 to 20 mMole/liter of a tenside of the bile acid group, and 0.1 to 10 g/liter of a non-ionic detergent. The pH is kept within a range of 5 to 9. In addition, a reagent is provided which is used for specific determination of HDL fraction cholesterol in the presence of the LDL fraction of serum lipoproteins. The reagent contains pancreatic esterase (0.05 to 30 U/ml); cholesterol oxidase (0.1 to 50 U/ml) a tenside of the bile acid grop (1.5 to 8 mMole/liter) and a non-ionic detergent (0.1 to 10 g/liter), all concentrations referring to the dilution used in the test. The reagent also contains a buffer at a pH of from 5 to 9, and a system for photometric determination of hydrogen peroxide.
摘要翻译：本发明提供了在血清脂蛋白的LDL级分存在下具体测定HDL级分的胆固醇的方法。胰胆固醇酯酶用于释放胆固醇，然后释放的胆固醇与胆固醇氧化酶和氧气反应形成过氧化氢。在胆固醇和氧化酶反应开始后2至15分钟内测量过氧化氢形成或氧气消耗的动力学。在预定的时间间隔内将温度保持在20℃的范围内。反应物的特定浓度保持在反应溶液中，即0.05至30U / ml胰胆固醇酯酶; 0.1至50U / ml胆固醇氧化酶; 1.0至20mMole /升的胆汁酸基的表面活性剂和0.1至10g /升的非离子型洗涤剂。 pH值保持在5〜9的范围。此外，提供了在血清脂蛋白的LDL级分存在下用于特异性测定HDL级分胆固醇的试剂。试剂含有胰酯酶（0.05〜30U / ml）; 胆固醇氧化酶（0.1至50U / ml）胆汁酸组织（1.5至8mMole /升）的表面活性剂和非离子型洗涤剂（0.1至10g /升），所有浓度均指试验中使用的稀释液。该试剂还含有pH为5至9的缓冲液和用于光度测定过氧化氢的系统。

10. 发明授权

US4578361A Creatinine antibody 失效
标题翻译：肌酸酐抗体
公开(公告)号：US4578361A
公开(公告)日：1986-03-25
申请号：US656957
申请日：1984-10-02
申请人： Joachim Siedel , Ulrich Neumann , Joachim Ziegenhorn , Hans-Georg Batz , Helmut Lenz , Brigitte Pautz , Winfried Albert
发明人： Joachim Siedel , Ulrich Neumann , Joachim Ziegenhorn , Hans-Georg Batz , Helmut Lenz , Brigitte Pautz , Winfried Albert
IPC分类号： G01N33/53 , A61K38/00 , C07K16/18 , G01N33/531 , G01N33/543 , G01N33/577 , G01N33/70 , A61K39/00
CPC分类号： C07K16/18 , G01N33/531 , G01N33/54306 , G01N33/577 , G01N33/70 , A61K38/00
摘要： The present invention provides a creatinine antibody and a process for the preparation thereof, wherein a conjugate of creatinine and a material suitable for antiserum formation, which are connected via an aliphatic or araliphatic carboxylic acid as bridge member, is used as immunogen for antiserum formation.The present invention is also concerned with the use of the above antibody for the immunological determination of creatinine, wherein the antibody is incubated with a creatinine-containing sample solution, reacted with a conjugate of creatinine with a hapten carrier substance, whereby one of the components, antibody and conjugate, is present in the solid phase or in dissolved form and the other component is present in dissolved form and the inhibition of the binding reaction between the antibody and the creatinine conjugate is measured.Furthermore, the present invention provides a reagent for the immunological determination of creatinine, wherein it contains the above antibody, a conjugate of creatinine with a hapten carrier substance and buffer substance.
摘要翻译：本发明提供一种肌酸酐抗体及其制备方法，其中通过脂肪族或脂环族羧酸作为桥接剂连接肌酸酐和适于抗血清形成的物质的缀合物，用作抗血清形成的免疫原。本发明还涉及上述抗体用于肌酸酐免疫学测定的用途，其中将抗体与含肌酸酐的样品溶液一起温育，与肌酸酐的缀合物与半抗原载体物质反应，由此其中一种成分，抗体和缀合物以固相或溶解形式存在，另一组分以溶解形式存在，并测量抗体与肌酐结合物之间结合反应的抑制。此外，本发明提供了用于肌酸酐免疫测定的试剂，其中含有上述抗体，肌酸酐与半抗原载体物质和缓冲物质的结合物。

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