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1. 发明授权

US5288606A Reagent for specific determination of fructosamine 失效
标题翻译：具体测定果糖胺的试剂
公开(公告)号：US5288606A
公开(公告)日：1994-02-22
申请号：US911282
申请日：1992-07-09
申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
发明人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/30 , C12Q1/44 , C12Q1/54 , G01N33/66 , C12Q1/00 , A61K35/78 , A61K37/02 , C12P19/38
CPC分类号： C12Q1/26 , G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： The present invention provides a process for the specific determination of the serum fructosamine content in blood or samples derived from blood by reaction with an appropriate color reagent and measurement of the color change thereby brought about, wherein, before the color reaction, non-specific reducing-acting and/or turbidity-causing sample components are removed at approximately neutral pH value, subsequently the pH is adjusted to a value of from 10 to 12 and the color reagent is added thereto.The present invention also provides a reagent mixture for the specific determination of the serum fructosamine content in blood or samples derived from blood, wherein it comprises a reagent for the removal of non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent with a buffer which has a pH value in the range of from 10.5 to 12.5 and a color reagent for the detection of fructosamine.
摘要翻译：本发明提供一种用于通过与适当的着色试剂反应并测量由此引起的颜色变化来特异性测定血液或血液中衍生血液的血清果糖胺含量的方法，其中在显色反应之前，非特异性还原在大约中性pH值下去除起泡和/或浊度的样品组分，随后将pH调节至10至12的值，并向其中加入着色剂。本发明还提供了用于特异性测定血液或血液中衍生血液中的果糖胺含量的试剂混合物，其中它包括用于除去非特异性还原作用和/或引起浊度的样品组分的试剂，具有pH值在10.5〜12.5范围内的缓冲液和用于检测果糖胺的显色试剂的缓冲试剂。

2. 发明授权

US5156947A Process for reduction of the matrix effect in a fructosamine determination assay 失效
标题翻译：降低木糖胺测定法中基质效应的方法
公开(公告)号：US5156947A
公开(公告)日：1992-10-20
申请号：US064336
申请日：1987-06-19
申请人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
发明人： Joachim Siedel , Joachim Ziegenhorn , Lieselotte Schellong , Bernd Vogt
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/30 , C12Q1/44 , C12Q1/54 , G01N33/66
CPC分类号： C12Q1/26 , G01N33/66 , Y10S436/825 , Y10S436/904 , Y10T436/144444 , Y10T436/25125
摘要： This invention teaches a process for reducing protein matrix effects in assays for serum fructosamine. Blood or blood derived samples are used, and one adds two reagents, one of which reduces interference caused by non-specific reducing substances, the other of which eliminates turbidity. Incubation follows, and then the pH of the sample is adjusted and color forming reagent is added. In one embodiment, the incubation time is only 1-15 minutes. In another embodiment, the first reagent contains peroxidase.
摘要翻译：本发明教导了一种在血清果糖胺测定中降低蛋白质基质效应的方法。使用血液或血液来源的样品，并加入两种试剂，其中之一减少了由非特异性还原物质引起的干扰，另外一种可以消除浊度。进行培养，然后调节样品的pH并加入成色试剂。在一个实施方案中，孵育时间仅为1-15分钟。在另一个实施方案中，第一试剂含有过氧化物酶。

3. 发明授权

US5055388A Process and reagent composition for determination of fructosamine in body fluids 失效
标题翻译：体液中木糖胺的测定方法及试剂组合物
公开(公告)号：US5055388A
公开(公告)日：1991-10-08
申请号：US190600
申请日：1988-05-05
申请人： Bernd Vogt , Lieselotte Schellong , Joachim Siedel , Joachim Ziegenhorn
发明人： Bernd Vogt , Lieselotte Schellong , Joachim Siedel , Joachim Ziegenhorn
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/54
CPC分类号： C12Q1/26 , C12Q1/54 , Y10S436/904 , Y10T436/144444 , Y10T436/25 , Y10T436/25125
摘要： A process for the determination of fructosamine in body fluids by the reaction of a sample solution with a color reagent, wherein the sample liquid is mixed with a buffer solution having a pH value of from 9 to 12, a color-forming reagent and uricase, as well as with at least one detergent, and the chronological change of the extinction is measured kinetically in a temperature range of from 20.degree. to 40.degree. C. at the earliest after 5 minutes.
摘要翻译：通过将样品溶液与着色试剂反应来测定体液中的果糖胺的方法，其中将样品液与pH值为9〜12的缓冲溶液，成色剂和尿酸酶混合，以及至少一种洗涤剂，并且在5分钟后最早在20℃至40℃的温度范围内动态地测量消光的时间顺序变化。

4. 发明授权

US4961970A Method for determining iron in a body fluid sample 失效
标题翻译：用于测定体液样品中铁的方法
公开(公告)号：US4961970A
公开(公告)日：1990-10-09
申请号：US238620
申请日：1988-08-30
申请人： Joachim Siedel , Lieselotte Schellong , Johnny Staepels , Uwe Herrmann , Michael-Harold Town
发明人： Joachim Siedel , Lieselotte Schellong , Johnny Staepels , Uwe Herrmann , Michael-Harold Town
IPC分类号： G01N33/84
CPC分类号： G01N33/84 , Y10S436/91
摘要： The present invention provides a process for the determination of iron in body fluids by liberation of the bound iron, reduction to Fe.sup.2+, addition of a color system appropriate for the detection of iron and photometric measurement in a tenside-containing sample solution, wherein a fatty acid polyethylene glycol ester, an alkanol polyglycol ether and at least 1 mole/liter quanidine hydrochloride are added to the sample solution. The present invention also provides a reagent for the determination of iron in serum, containing a reducing agent, a color material system appropriate for the detection of iron and guanidine hydrochloride and at least one tenside, wherein it contains a fatty acid polyethylene glycol ester and a alkanol polyglycol ether.
摘要翻译：本发明提供了一种通过释放结合的铁，还原成Fe2 +，添加适合于检测铁的颜色系统和在含有表面活性剂的样品溶液中进行光度测量来测定体液中铁的方法，其中脂肪酸性聚乙二醇酯，链烷醇聚乙二醇醚和至少1摩尔/升盐酸氢化物加入到样品溶液中。本发明还提供了一种用于测定血清中含铁的试剂，其含有还原剂，适用于检测铁和胍盐酸盐和至少一种表面活性剂的着色材料体系，其中它含有脂肪酸聚乙二醇酯和链烷醇聚乙二醇醚。

5. 发明授权

US06207459B1 Method for the analysis of medical samples containing haemoglobin 有权
标题翻译：分析含有血红蛋白的医学样品的方法
公开(公告)号：US06207459B1
公开(公告)日：2001-03-27
申请号：US09147288
申请日：1998-11-20
申请人： Ralph Weisheit , Lieselotte Schellong
发明人： Ralph Weisheit , Lieselotte Schellong
IPC分类号： G01N3372
CPC分类号： G01N33/52 , G01N33/6827 , G01N33/72 , G01N33/84
摘要： The invention concerns a method for the determination of an analyte in a sample containing free haemoglobin in which the determination is carried out by an optical measurement and the value measured for the analyte concentration is mathematically corrected. This method is in particular suitable for determining the parameters total protein, iron and albumin in a medical sample e.g. in a serum or plasma sample. The correction of the measured value for the analyte concentration is achieved by the steps (a) measuring the blank value of the sample to be analysed, (b) measuring the blank value of a haemoglobin-free reference sample, (c) measuring the uncorrected value for the analyte concentration and (d) correcting the value obtained in step (c) by correlation with the values obtained in step (a) and (b) in order to obtain the corrected value for the analyte concentration.
摘要翻译：本发明涉及一种用于测定含有游离血红蛋白的样品中的分析物的方法，其中通过光学测量进行测定，并且数学地校正测量的分析物浓度的值。该方法特别适用于确定医学样品中的总蛋白，铁和白蛋白的参数，例如。在血清或血浆样品中。通过步骤（a）测量要分析的样品的空白值，（b）测量无血红蛋白参考样品的空白值，（c）测量未校正的样品，测量分析物浓度的测量值的校正（d）通过与步骤（a）和（b）中获得的值相关来校正步骤（c）中获得的值，以获得分析物浓度的校正值。

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