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1. 发明授权

US5443954A Immortalized non-tumorigenic human bronchial epithelial cell lines 失效
标题翻译：永生化的非致瘤性人支气管上皮细胞系
公开(公告)号：US5443954A
公开(公告)日：1995-08-22
申请号：US636712
申请日：1991-01-02
申请人： Roger R. Reddel , Yang Ke , Johng S. Rhim , Douglas E. Brash , Robert T. Su , John F. Lechner , Brenda I. Gerwin , Curtis C. Harris , Paul Amstad
发明人： Roger R. Reddel , Yang Ke , Johng S. Rhim , Douglas E. Brash , Robert T. Su , John F. Lechner , Brenda I. Gerwin , Curtis C. Harris , Paul Amstad
IPC分类号： C12N5/10 , C12N5/071 , C12N9/02 , C12N15/09 , C12Q1/02 , G01N33/50
CPC分类号： C12N9/0071 , C12N5/0688 , G01N33/5011 , G01N33/5017 , C12N2500/90
摘要： Immortalized human bronchial epithelial and human mesothelial cell lines have been obtained. Various uses of these cell lines have been described.
摘要翻译：已经获得了永生化的人支气管上皮和人间皮细胞系。已经描述了这些细胞系的各种用途。

2. 发明授权

US4885238A Immortalized human bronchial epitherial mesothelial cell lines 失效
标题翻译：永生化人支气管上皮细胞间皮细胞系
公开(公告)号：US4885238A
公开(公告)日：1989-12-05
申请号：US114508
申请日：1987-10-30
申请人： Roger R. Reddel , Ke Yang , Johng S. Rhim , Douglas Brash , Robert T. Su , John F. Lechner , Brenda I. Gerwin , Curtis C. Harris , Paul Amstad
发明人： Roger R. Reddel , Ke Yang , Johng S. Rhim , Douglas Brash , Robert T. Su , John F. Lechner , Brenda I. Gerwin , Curtis C. Harris , Paul Amstad
IPC分类号： C12N5/071 , C12N9/02 , G01N33/50
CPC分类号： C12N9/0071 , C12N5/0688 , G01N33/5011 , G01N33/5017 , C12N2500/90 , Y10S435/948
摘要： Immortalized human bronchial epithelial and human mesothelial cell lines have been obtained. Various uses of these cell lines have been described.
摘要翻译：已经获得了永生化的人支气管上皮和人间皮细胞系。已经描述了这些细胞系的各种用途。

3. 发明授权

US5665589A Human liver epithelial cell lines 失效
标题翻译：人肝上皮细胞系
公开(公告)号：US5665589A
公开(公告)日：1997-09-09
申请号：US25336
申请日：1993-03-03
申请人： Curtis C. Harris , Katharine H. Cole , John F. Lechner , Roger Reddel
发明人： Curtis C. Harris , Katharine H. Cole , John F. Lechner , Roger Reddel
IPC分类号： C12N5/071 , G01N33/50 , C12N5/06 , C12N5/10
CPC分类号： G01N33/5014 , C12N5/067 , G01N33/5017 , C12N2500/14 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/32 , C12N2500/84 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2503/02 , C12N2510/04 , G01N2333/914
摘要： Immortalized cell lines derived from normal adult human liver are described which express phenotypic characteristics of normal adult liver epithelial cells.
摘要翻译：描述了从正常成年人肝脏衍生的永生化细胞系，其表达正常成年肝上皮细胞的表型特征。

4. 发明授权

US4608339A Protoplast fusion method for high-frequency DNA transfection in human cells 失效
标题翻译：人体细胞高频DNA转染的原生质体融合方法
公开(公告)号：US4608339A
公开(公告)日：1986-08-26
申请号：US545257
申请日：1983-10-25
申请人： George H. Yoakum , Curtis C. Harris , Brent E. Korba , John F. Lechner
发明人： George H. Yoakum , Curtis C. Harris , Brent E. Korba , John F. Lechner
IPC分类号： C07K14/02 , C12N5/16 , C12N15/87 , C12N15/00 , C12N5/00 , C12P21/02
CPC分类号： C07K14/005 , C12N15/87 , C12N5/16 , C12N2510/00 , C12N2730/10122
摘要： A modified protoplast fusion method and cell line is disclosed that stably transfects human cells with pSV2-derived plasmids at frequencies greater than 10.sup.-3. This procedure makes it possible to test the biological effect of individual genes (i.e., oncogenes and other cellular genes, and viral genes). To demonstrate the utility of this invention, a pSV2gpt.sup.+ plasmid constructed to carry a subgenomic fragment of hepatitis B virus (HBV) that contained the core antigen gene (HBc gene) is transfected into human cells. Human cell lines are stably transfected with the HBC.sup.+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression; other selective markers, i.e., neomycin resistance, can also be used. Conditions for enhancing the expression of the transfected gene(s) have also been developed. For example, with this gpt.sup.+ /HBc.sup.+ cell line it is shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen.
摘要翻译：公开了修饰的原生质体融合方法和细胞系，其以大于10-3的频率以pSV2衍生的质粒稳定转染人细胞。该方法可以测试各基因（即癌基因和其他细胞基因以及病毒基因）的生物学效应。为了证明本发明的实用性，将构建用于携带含有核心抗原基因（HBc基因）的乙型肝炎病毒（HBV）亚基因组片段的pSV2gpt +质粒转染入人细胞。通过选择用于表达鸟嘌呤磷酸核糖转移酶表达的受体细胞，用HBC +基因稳定转染人细胞系; 也可以使用其他选择标记，即新霉素抗性。还开发了用于增强转染基因表达的条件。例如，使用这种gpt + / HBc +细胞系，显示无血清培养基的生长或用5'-氮杂胞苷碱处理刺激HBV核心抗原的产生。

5. 发明授权

US5529920A Human liver epithelial cell line and culture media therefor 失效
标题翻译：人肝上皮细胞系及其培养基
公开(公告)号：US5529920A
公开(公告)日：1996-06-25
申请号：US879165
申请日：1992-05-01
申请人： Katharine H. Cole , John F. Lechner , Roger Reddel , Curtis C. Harris , Andrea M. Pfeifer
发明人： Katharine H. Cole , John F. Lechner , Roger Reddel , Curtis C. Harris , Andrea M. Pfeifer
IPC分类号： C12N5/071 , C12Q1/04 , G01N33/50 , C12N5/06 , A01N63/00 , C12N5/10 , C12Q1/02
CPC分类号： C12N5/067 , C12Q1/045 , G01N33/5017 , C12N2500/14 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/32 , C12N2500/84 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2510/04 , G01N2333/914
摘要： The present invention relates to long term multiplication and permanent establishment of a cell line of human liver epithelial cells(hepatocytes). The human liver epithelial cell line is capable of mitotically proliferating and continuously growing in vitro under suitable environmental conditions in suitable culture media. A method of producing an immortalized human liver epithelial cell line is also disclosed. The invention also relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
摘要翻译：本发明涉及长期繁殖和永久建立人肝上皮细胞（肝细胞）的细胞系。人类肝上皮细胞系能够在合适的环境条件下在合适的培养基中体外增殖并连续生长。还公开了永生化人肝上皮细胞系的制备方法。本发明还涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的无血清细胞培养基。介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。

6. 发明授权

US5759765A Human liver epithelial cell lines 失效
标题翻译：人肝上皮细胞系
公开(公告)号：US5759765A
公开(公告)日：1998-06-02
申请号：US458878
申请日：1995-06-02
申请人： Curtis C. Harris , Katharine H. Cole , John F. Lechner , Roger Reddel
发明人： Curtis C. Harris , Katharine H. Cole , John F. Lechner , Roger Reddel
IPC分类号： C12N5/071 , G01N33/50 , C12N7/00
CPC分类号： G01N33/5014 , C12N5/067 , G01N33/5017 , C12N2500/14 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/32 , C12N2500/84 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2503/02 , C12N2510/04 , G01N2333/914
摘要： Immortalized cell lines derived from normal adult human liver are described which express phenotypic characteristics of normal adult liver epithelial cells. The invention further provides methods of using the immortalized cells to evaluate the cytotoxicity or carcinogenicity of a compound.
摘要翻译：描述了从正常成年人肝脏衍生的永生化细胞系，其表达正常成年肝上皮细胞的表型特征。本发明还提供了使用永生化细胞来评价化合物的细胞毒性或致癌性的方法。

7. 发明授权

US5342777A Cell culture medium for human liver epithelial cell line 失效
标题翻译：人肝上皮细胞系细胞培养液
公开(公告)号：US5342777A
公开(公告)日：1994-08-30
申请号：US844873
申请日：1992-03-03
申请人： Katharine H. Cole , John F. Lechner , Curtis C. Harris
发明人： Katharine H. Cole , John F. Lechner , Curtis C. Harris
IPC分类号： C12N5/071 , C12Q1/04 , G01N33/50 , C12N1/00 , C12N5/00 , C12N15/00 , C12Q1/00
CPC分类号： G01N33/5017 , C12N5/067 , C12Q1/045 , C12N2500/14 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/32 , C12N2500/84 , C12N2500/90 , C12N2501/01 , C12N2501/11 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2510/04 , G01N2333/914 , Y10S435/948
摘要： The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
摘要翻译：本发明涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的细胞培养基。介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。

8. 发明申请

US20100136541A1 IDENTIFICATION AND VERIFICATION OF METHYLATION MARKER SEQUENCES 审中-公开
标题翻译：甲基标记序列的鉴定和验证
公开(公告)号：US20100136541A1
公开(公告)日：2010-06-03
申请号：US12489502
申请日：2009-06-23
申请人： Chris Beard , Chris Burgess , Allison Gannon , Jeanne Harvey , John F. Lechner , Zheng Li
发明人： Chris Beard , Chris Burgess , Allison Gannon , Jeanne Harvey , John F. Lechner , Zheng Li
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6809 , C12Q1/6827 , C12Q2600/112 , C12Q2600/136 , C12Q2600/154 , C12Q2523/125 , C12Q2565/501
摘要： The present invention relates to methods for identifying among the genes that are down-regulated in cells or tissues having disease including cancer, the CpG sites within the CpG islands of said genes, wherein the identified CpG sites show great potential for diagnostic utility. In another aspect, the present invention also provides methods of using the selected CpG sites for purposes of diagnosis, prognosis, staging, assessing or monitoring the therapy of or recovery from a disease such as cancer.
摘要翻译：本发明涉及在具有疾病的细胞或组织中被下调的基因中鉴定的方法，所述基因在所述基因的CpG岛内具有CpG位点，其中所鉴定的CpG位点具有很大的诊断效用潜力。另一方面，本发明还提供了使用所选择的CpG位点用于诊断，预后，分期，评估或监测疾病如癌症的治疗或恢复的方法。

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