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1. 发明授权

US6037152A Method for reducing carryover contamination in an amplification procedure 失效
标题翻译：在扩增过程中减少残留污染的方法
公开(公告)号：US6037152A
公开(公告)日：2000-03-14
申请号：US376763
申请日：1995-01-23
申请人： Rodney M. Richards , Theodore Jones , Gregory S. Brown
发明人： Rodney M. Richards , Theodore Jones , Gregory S. Brown
IPC分类号： C12N15/09 , C12N15/00 , C12Q1/68 , C12Q1/70 , C12P19/34 , C12N9/14 , C12N9/24
CPC分类号： C12Q1/6848 , C12Q1/6862 , C12Q1/703 , Y10S435/81
摘要： The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.
摘要翻译：本发明提供了一种在放大过程中减少携带污染的有效且经济的方法。通过将至少一种修饰引入扩增产物，本发明的方法能够降低或消除由污染物扩增产物引起的背景。修饰的扩增产物可以容易地与测试样品中的靶序列区分开。在放大新的测试样品中的靶之前，可以处理样品以选择性地消除污染物扩增产物，使得其不能在新样品中扩增。

2. 发明授权

US5876976A Method for reducing carryover contamination in an amplification procedure 失效
公开(公告)号：US5876976A
公开(公告)日：1999-03-02
申请号：US376762
申请日：1995-01-23
申请人： Rodney M. Richards , Theodore Jones , David L. Snitman , Gregory S. Brown
发明人： Rodney M. Richards , Theodore Jones , David L. Snitman , Gregory S. Brown
IPC分类号： C12N15/09 , C12N15/00 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6862 , C12Q1/703 , Y10S435/81
摘要： The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.

3. 发明授权

US5650302A Method for reducing carryover contamination in an amplification procedure 失效
标题翻译：在扩增过程中减少残留污染的方法
公开(公告)号：US5650302A
公开(公告)日：1997-07-22
申请号：US469085
申请日：1995-06-05
申请人： Rodney M. Richards , Theodore Jones , Gregory S. Brown
发明人： Rodney M. Richards , Theodore Jones , Gregory S. Brown
IPC分类号： C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/6848
摘要： The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.
摘要翻译：本发明提供了一种在放大过程中减少携带污染的有效且经济的方法。通过将至少一种修饰引入扩增产物，本发明的方法能够降低或消除由污染物扩增产物引起的背景。修饰的扩增产物可以容易地与测试样品中的靶序列区分开。在放大新的测试样品中的靶之前，可以处理样品以选择性地消除污染物扩增产物，使得其不能在新样品中扩增。

4. 发明授权

US5427929A Method for reducing carryover contamination in an amplification procedure 失效
标题翻译：在扩增过程中减少残留污染的方法
公开(公告)号：US5427929A
公开(公告)日：1995-06-27
申请号：US057192
申请日：1993-05-03
申请人： Rodney M. Richards , Theodore Jones , David L. Snitman , Gregory S. Brown
发明人： Rodney M. Richards , Theodore Jones , David L. Snitman , Gregory S. Brown
IPC分类号： C12N15/09 , C12N15/00 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6862 , C12Q1/703 , Y10S435/81
摘要： The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively cleave the contaminant amplification product so that it cannot be amplified in the new sample.
摘要翻译：本发明提供了一种在放大过程中减少携带污染的有效且经济的方法。通过将至少一种修饰引入扩增产物，本发明的方法能够减少或消除由污染物扩增产物引起的背景。修饰的扩增产物可以容易地与测试样品中的靶序列区分开。在放大新的测试样品中的靶之前，可以处理样品以选择性地切割污染物扩增产物，使得其不能在新样品中扩增。

5. 发明授权

US07285401B1 Method and reagents for detecting nucleic acid sequences 失效
标题翻译：用于检测核酸序列的方法和试剂
公开(公告)号：US07285401B1
公开(公告)日：2007-10-23
申请号：US08480005
申请日：1995-06-07
申请人： Theodore Jones , Rodney M. Richards
发明人： Theodore Jones , Rodney M. Richards
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6844
摘要： The present invention is directed to a method for detecting an amplification product. Amplification is accomplished through the use of a plurality of detection probes. The present invention is also directed to a method for detecting a target nucleic acid sequence by contacting a test sample with an excess of at least three pairs of nucleic acid amplification probes.
摘要翻译：本发明涉及一种检测扩增产物的方法。通过使用多个检测探针实现扩增。本发明还涉及通过使测试样品与过量的至少三对核酸扩增探针接触来检测靶核酸序列的方法。

6. 发明授权

US5650271A Enzymatic synthesis of oligonucleotides 失效
公开(公告)号：US5650271A
公开(公告)日：1997-07-22
申请号：US41631
申请日：1993-04-01
申请人： Rodney M. Richards
发明人： Rodney M. Richards
IPC分类号： C12N15/09 , A61K20060101 , A61K31/70 , C07H20060101 , C07H1/00 , C07H15/12 , C07H21/00 , C12N15/10 , C12P20060101 , C12P19/34 , C12Q20060101 , C12Q1/68 , G01N20060101 , G01N33/566 , G01N33/58
CPC分类号： C12N15/10 , C12Q1/682 , C12Q1/6853 , Y02P20/582
摘要： The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.

7. 发明授权

US5863732A Diagnostic kits for detection of target nucleic acid sequences 失效
标题翻译：用于检测靶核酸序列的诊断试剂盒
公开(公告)号：US5863732A
公开(公告)日：1999-01-26
申请号：US769601
申请日：1996-12-18
申请人： Rodney M. Richards
发明人： Rodney M. Richards
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6853 , C12N15/10 , C12Q1/682 , C12Q1/6823 , Y02P20/582 , Y10S435/975
摘要： The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.
摘要翻译：本发明通过使用特别设计的模板序列使得序列特异性寡核苷酸的催化生成成为可能。反应可以在过量的核苷三磷酸，聚合剂和切割剂的存在下进行等温进行。因为该方法对于模板序列是催化的，所以理论上可以从单个模板分子产生无限量的寡核苷酸产物。当通过存在核酸靶序列引发该过程时，本发明的方法可用作诊断目的，作为增强灵敏度的扩增方法。通过采用这些模板序列的级联可以进一步提高诊断灵敏度。

8. 发明授权

US5645987A Enzymatic synthesis of oligonucleotides 失效
标题翻译：寡核苷酸的酶合成
公开(公告)号：US5645987A
公开(公告)日：1997-07-08
申请号：US200897
申请日：1994-02-23
申请人： Rodney M. Richards
发明人： Rodney M. Richards
IPC分类号： C12N15/10 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6853 , C12N15/10 , C12Q1/682 , C12Q1/6823 , Y02P20/582 , Y10S435/975
摘要： The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.
摘要翻译：本发明通过使用特别设计的模板序列使得序列特异性寡核苷酸的催化生成成为可能。反应可以在过量的核苷三磷酸，聚合剂和切割剂的存在下进行等温进行。因为该方法对于模板序列是催化的，所以理论上可以从单个模板分子产生无限量的寡核苷酸产物。当通过存在核酸靶序列引发该过程时，本发明的方法可用于诊断目的，作为增强灵敏度的扩增方法。通过采用这些模板序列的级联可以进一步提高诊断灵敏度。

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