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1. 发明授权

US06106825A Entomopoxvirus-vertebrate gene delivery vector and method 失效
标题翻译：昆虫毒素 - 脊椎动物基因递送载体和方法
公开(公告)号：US06106825A
公开(公告)日：2000-08-22
申请号：US852629
申请日：1997-05-07
申请人： Richard W. Moyer , Yi Li , Richard L. Hall
发明人： Richard W. Moyer , Yi Li , Richard L. Hall
IPC分类号： C12N7/01 , C12N15/863 , A01N63/00 , C12N5/10 , C12N15/63
CPC分类号： C12N15/86 , A61K2039/51 , C12N2710/24043
摘要： This invention provides recombinant entomopoxvirus as a novel vector and method of using such vector for delivery and expression of genes of biological significance to vertebrate cells, including human cells. This invention includes the use of an early entomopoxvirus or like gene promoter to drive the expression of an heterologous gene product in a vertebrate cell infected with the recombinant entomopoxvirus vector. We have discovered that, while the entomopoxvirus vector of this invention does not replicate in the vertebrate cell, it enters vertebrate cell cytoplasm and achieves expression of an heterologous gene inserted into the rEPV without causing cytopathic or cytotoxic effects. We further provide a method for expression of the heterologous gene under control of late, strong entomopoxvirus gene promoters, vertebrate gene or viral promoters, and for stable integration of the heterologous gene delivered by the rEPV into the vertebrate cell.
摘要翻译：本发明提供了重组昆虫痘病毒作为新的载体和使用这种载体递送和表达对包括人细胞在内的脊椎动物细胞具有生物学意义的基因的基因的方法。本发明包括使用早期昆虫病毒或类似基因启动子来驱动感染重组昆虫病毒载体的脊椎动物细胞中异源基因产物的表达。我们已经发现，虽然本发明的昆虫病毒载体在脊椎动物细胞中不复制，但是它进入脊椎动物细胞质，并且实现插入到rEPV中的异源基因的表达，而不引起细胞病变或细胞毒性作用。我们进一步提供了一种在晚期，强烈的昆虫痘病毒基因启动子，脊椎动物基因或病毒启动子的控制下表达异源基因的方法，并且将由rEPV递送的异源基因稳定整合到脊椎动物细胞中。

2. 发明授权

US5935777A Entomopoxvirus expression system 失效
公开(公告)号：US5935777A
公开(公告)日：1999-08-10
申请号：US544332
申请日：1995-10-17
申请人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl , Yi Li
发明人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl , Yi Li
IPC分类号： C07K14/065 , C12N9/12 , C12N15/65 , C12N15/85 , C12N15/863 , C12Q1/70 , C07H21/04 , C12N15/86 , C12P21/00
CPC分类号： C12N15/86 , C07K14/005 , C12N15/65 , C12N15/85 , C12N9/1211 , C12N2710/24022 , C12N2710/24043 , C12N2710/24143 , C12N2830/00 , C12N2830/60
摘要： The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.

3. 发明授权

US5721352A Entomopoxvirus expression system 失效
公开(公告)号：US5721352A
公开(公告)日：1998-02-24
申请号：US107755
申请日：1993-11-22
申请人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl
发明人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl
IPC分类号： C07K14/52 , C07K14/00 , C07K14/065 , C07K14/555 , C07K14/565 , C07K19/00 , C12N5/10 , C12N7/00 , C12N9/12 , C12N15/09 , C12N15/65 , C12N15/85 , C12N15/863 , C12P21/02 , C12R1/92 , C07H21/04
CPC分类号： C07K14/005 , C12N15/65 , C12N15/85 , C12N15/86 , C12N9/1211 , C12N2710/24022 , C12N2710/24143
摘要： The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.

4. 发明授权

US5476781A Entomopoxvirus spheroidin gene sequences 失效
标题翻译：昆虫痘病毒球状蛋白基因序列
公开(公告)号：US5476781A
公开(公告)日：1995-12-19
申请号：US991867
申请日：1992-12-07
申请人： Richard W. Moyer , Richard L. Hall
发明人： Richard W. Moyer , Richard L. Hall
IPC分类号： C07K14/52 , C07K14/00 , C07K14/065 , C07K14/555 , C07K14/565 , C07K19/00 , C12N5/10 , C12N7/00 , C12N9/12 , C12N15/09 , C12N15/40 , C12N15/65 , C12N15/85 , C12N15/863 , C12P21/02 , C12R1/92 , C12N15/11 , C12N15/39 , C12N15/62
CPC分类号： C12N15/86 , C07K14/005 , C12N15/65 , C12N15/85 , C12N9/1211 , C12N2710/24022 , C12N2710/24043 , C12N2710/24143
摘要： The subject invention pertains to novel Entomopoxvirus (EPV) spheroidin polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.
摘要翻译：本发明涉及与其与天然结合的其他病毒序列无关的新型昆虫痘病毒（EPV）球状蛋白多核苷酸序列，含有该序列的重组多核苷酸载体，含有该序列的重组病毒以及用该重组病毒感染的宿主细胞以及用于在昆虫和哺乳动物宿主细胞中表达异源蛋白质的方法。

5. 发明授权

US06410221B1 Entomopoxvirus expression system 失效
标题翻译：昆虫毒素表达系统
公开(公告)号：US06410221B1
公开(公告)日：2002-06-25
申请号：US09370861
申请日：1999-08-09
申请人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl , Yi Li
发明人： Richard W. Moyer , Richard L. Hall , Michael E. Gruidl , Yi Li
IPC分类号： C12Q168
CPC分类号： C12N15/86 , C07K14/005 , C12N9/1211 , C12N15/65 , C12N15/85 , C12N2710/24022 , C12N2710/24043 , C12N2710/24143 , C12N2830/00 , C12N2830/60
摘要： The subject invention pertains to novel Entomopoxvirus (EPV) polynucleotide sequences free from association with other viral sequences with which they are naturally associated, recombinant polynucleotide vectors containing the sequences, recombinant viruses containing the sequences, and host cells infected with the recombinant viruses are provided herein, as well as methods for use thereof in the expression of heterologous proteins in both insect and mammalian host cells.
摘要翻译：本发明涉及与其与天然结合的其他病毒序列无关的新型昆虫痘病毒（EPV）多核苷酸序列，本文提供了含有该序列的重组多核苷酸载体，含有该序列的重组病毒和用该重组病毒感染的宿主细胞，以及用于在昆虫和哺乳动物宿主细胞中表达异源蛋白质的方法。

6. 发明授权

US6127172A Materials and methods for delivery and expression of heterologous DNA in vertebrate cells 失效
标题翻译：用于在脊椎动物细胞中递送和表达异源DNA的材料和方法
公开(公告)号：US6127172A
公开(公告)日：2000-10-03
申请号：US86651
申请日：1998-05-29
申请人： Richard W. Moyer , Yi Li
发明人： Richard W. Moyer , Yi Li
IPC分类号： A61K48/00 , A61P35/00 , C12N7/01 , C12N5/10 , C12N15/00
CPC分类号： A61K48/00
摘要： The subject invention pertains to recombinant entomopox vectors which are useful for the delivery and stable expression of heterologous DNA in vertebrate cells. Specifically exemplified is a recombinant EPV from amsacta moorei (AmEPV). Because of the capacity of the EPV to incorporate foreign or heterologous DNA sequences, the vectors of the subject invention can be used to deliver DNA inserts that are larger than 10 kb in size. Accordingly, one aspect of the present invention concerns use of the recombinant vectors for delivery and expression of biological useful proteins in gene therapy protocols.
摘要翻译：本发明涉及可用于在脊椎动物细胞中递送和稳定表达异源DNA的重组昆虫病毒载体。具体举例说明的是来自Amosacta moorei（AmEPV）的重组EPV。由于EPV引入外来或异源DNA序列的能力，本发明的载体可用于递送大于10kb的DNA插入片段。因此，本发明的一个方面涉及重组载体在基因治疗方案中递送和表达生物有用蛋白质的应用。

7. 发明授权

US06217882B1 Use of recombinant swine poxvirus as a live vaccine vector 失效
标题翻译：使用重组猪痘病毒作为活疫苗载体
公开(公告)号：US06217882B1
公开(公告)日：2001-04-17
申请号：US09299268
申请日：1999-04-26
申请人： Richard W. Moyer , Eladio Viñuela , E. P. J. Gibbs
发明人： Richard W. Moyer , Eladio Viñuela , E. P. J. Gibbs
IPC分类号： A61K39275
CPC分类号： C12N15/86 , A61K38/00 , A61K39/00 , C12N2710/16734 , C12N2710/24034 , C12N2710/24043
摘要： The present invention provides a recombinant swinepox virus vector containing a heterologous nucleotide sequence encoding a protein from a selected pathogen inserted into, or replacing, all or a portion of a swinepox virus gene, which gene is not essential to replication of the virus in a host cell. Also provided is a recombinant SPV vector into which a pseudorabies antigen is inserted within the TK gene, which is useful in diagnostic, therapeutic, and prophylactic compositions.
摘要翻译：本发明提供了一种重组羊膜蛋白病毒载体，其含有编码来自插入或替代全部或一部分羊膜蛋白病毒基因的选定病原体的蛋白质的异源核苷酸序列，该基因对病毒在宿主中的复制不是必需的细胞。还提供了在TK基因中插入伪狂犬病抗原的重组SPV载体，其可用于诊断，治疗和预防组合物。

8. 发明授权

US5651972A Use of recombinant swine poxvirus as a live vaccine vector 失效
标题翻译：使用重组猪痘病毒作为活疫苗载体
公开(公告)号：US5651972A
公开(公告)日：1997-07-29
申请号：US307499
申请日：1994-09-14
申请人： Richard W. Moyer , Eladio Vinuela , E. P. J. Gibbs
发明人： Richard W. Moyer , Eladio Vinuela , E. P. J. Gibbs
IPC分类号： A61K38/00 , A61K39/00 , A61P31/00 , C12N7/01 , C12N15/863 , A61K39/275 , A61K39/245 , C12N15/86
CPC分类号： C12N15/86 , A61K38/00 , A61K39/00 , C12N2710/16734 , C12N2710/24034 , C12N2710/24043
摘要： The present invention provides a recombinant swinepox virus vector containing a heterologous nucleotide sequence encoding a protein from a selected pathogen inserted into, or replacing, all or a portion of a swinepox virus gene, which gene is not essential to replication of the virus in a host cell. Also provided is a recombinant SPV vector into which a pseudorabies antigen is inserted within the TK gene, which is useful in diagnostic, therapeutic, and prophylactic compositions.
摘要翻译：本发明提供了一种重组羊膜蛋白病毒载体，其含有编码来自插入或替代全部或一部分羊膜蛋白病毒基因的选定病原体的蛋白质的异源核苷酸序列，该基因对病毒在宿主中的复制不是必需的细胞。还提供了在TK基因中插入伪狂犬病抗原的重组SPV载体，其可用于诊断，治疗和预防组合物。

9. 发明授权

US5212057A Biological system for constructing and testing viral vaccines 失效
标题翻译：用于构建和测试病毒疫苗的生物系统
公开(公告)号：US5212057A
公开(公告)日：1993-05-18
申请号：US455483
申请日：1989-12-22
申请人： Richard W. Moyer , David C. Bloom
发明人： Richard W. Moyer , David C. Bloom
IPC分类号： C12N15/863 , C12P7/00 , C12Q1/70
CPC分类号： C12N15/86 , C12P7/00 , C12Q1/70 , C12N2710/24143 , C12N2710/24145
摘要： Disclosed is a novel biological system for 1) the evaluation of potential complications of live virus vaccines prior to actual testing in animals or in the field, and 2) the construction of vaccine vectors in which the ability to grow within specific organs or tissues has selectively been eliminated from the virus.
摘要翻译：公开了一种新颖的生物系统，用于1）在动物或现场实际测试之前评估活病毒疫苗的潜在并发症，以及2）在特定器官或组织内生长能力有选择性的疫苗载体的构建被从病毒中消除。

10. 发明授权

US06933145B1 Materials and methods for delivery and expression of heterologous DNA in vertebrate cells 失效
标题翻译：用于在脊椎动物细胞中递送和表达异源DNA的材料和方法
公开(公告)号：US06933145B1
公开(公告)日：2005-08-23
申请号：US09662254
申请日：2000-09-14
申请人： Richard W. Moyer , Yi Li
发明人： Richard W. Moyer , Yi Li
IPC分类号： A61K48/00 , C07K14/065 , C12N5/08 , C12N15/863 , C12N15/87 , C07H21/04 , C12N15/00
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/24022 , C12N2710/24122 , C12N2710/24143
摘要： The subject invention pertains to recombinant entomopox vectors which are useful for the delivery and stable expression of heterologous DNA in vertebrate cells. Specifically exemplified is a recombinant EPV from amsacta moorei (AmEPV). Because of the capacity of the EPV to incorporate foreign or heterologous DNA sequences, the vectors of the subject invention can be used to deliver DNA inserts that are larger than 10 kb in size. Accordingly, one aspect of the present invention concerns use of the recombinant vectors for delivery and expression of biological useful proteins in gene therapy protocols. In addition, the subject invention concerns novel AmEVP polypeptides and the polynucleotide sequences which encode these polypeptides.
摘要翻译：本发明涉及可用于在脊椎动物细胞中递送和稳定表达异源DNA的重组昆虫病毒载体。具体举例说明的是来自Amosacta moorei（AmEPV）的重组EPV。由于EPV引入外来或异源DNA序列的能力，本发明的载体可用于递送大于10kb的DNA插入片段。因此，本发明的一个方面涉及重组载体在基因治疗方案中递送和表达生物有用蛋白质的应用。此外，本发明涉及新型AmEVP多肽和编码这些多肽的多核苷酸序列。

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