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1. 发明授权

US5852175A P-selectin glycoprotein ligand blocking antibodies 失效
标题翻译： P-选择素糖蛋白配体阻断抗体
公开(公告)号：US5852175A
公开(公告)日：1998-12-22
申请号：US438280
申请日：1995-05-10
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K38/00 , C07K14/705 , C07K16/28 , C07K16/18
CPC分类号： C07K16/2896 , C07K14/705 , C07K14/70564 , C07K14/70596 , C07K16/2854 , A61K2039/505 , A61K38/00
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of �.sup.3 H!glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
摘要翻译：已经证明P-选择蛋白主要结合中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P-选择素的亲和层析进行评估时。该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂。

2. 发明授权

US6124267A O-glycan inhibitors of selectin mediated inflammation derived from PSGL-1 失效
标题翻译：来自PSGL-1的选择素介导的炎症的O-聚糖抑制剂
公开(公告)号：US6124267A
公开(公告)日：2000-09-26
申请号：US63237
申请日：1998-04-20
申请人： Rodger P. McEver , Richard D. Cummings , Kevin L. Moore
发明人： Rodger P. McEver , Richard D. Cummings , Kevin L. Moore
IPC分类号： C07K14/705 , C07K16/28 , A61K31/70
CPC分类号： C07K16/2896 , C07K14/705 , C07K2317/34
摘要： Tyrosine sulfate on PSGL-1, particularly at least one of residues 46, 48 and 51, functions in conjunction with sialylated and fucosylated glycans, most preferably Thr-57, to mediate high affinity binding to P-selectin. PSGL-1 O-glycans have been determined to consist of disialylated or neutral forms of the core-2 tetrasaccharide Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.6(Gal.beta.1.fwdarw.3)GalNAcOH. A minority of the O-glycans are .alpha.1,3 fucosylated that occur as two major species containing the sialyl Lewis x antigen--one species is a disialylated monofucosylated glycan: Fuc.alpha.1 .dwnarw. 3 NeuAc.alpha.2.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1 .dwnarw. 6 NeuAc.alpha.2.fwdarw.3Gal.beta.1.fwdarw.3GalNAc-R, and the other is a monosialylated, trifucosylated glycan having a polylactosamine backbone: Fuc.alpha.l .dwnarw. 3 NeuAc.alpha.2.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw. Fuc.alpha.l Fuc.alpha.l .dwnarw. .dwnarw. 3 3 4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1 .dwnarw. 6 Gal.beta.1.fwdarw.3GalNAc-R wherein R=H, OH, another sugar or an aglycone such as an amino acid, peptide, or polypeptide. The O-glycans defined herewith can be used to inhibit inflammation mediated by P-selectin.
摘要翻译： PSGL-1上的酪氨酸硫酸盐，特别是残基46,48和51中的至少一个，与唾液酸化和岩藻糖基化聚糖结合起来，最优选Thr-57，以介导与P-选择素的高亲和力结合。已经确定PSGL-1 O-聚糖由二核苷酸或中性形式的核心二糖Galβ1-> 4GlcNAcβ1-> 6（Galβ1-> 3）GalNAcOH组成。少数O聚糖是α1,3-岩藻糖基化的，它们作为含有唾液酸基路易斯x抗原的两种主要物质发生，一种是二唾液酸化单糖基化聚糖： - Fucα1 - ＆darr＆ - 3 - NeuAc alpha 2-> 3Gal beta 1-> 4GlcNAc beta 1 - ＆darr＆ - 6 - NeuAc alpha 2-> 3Gal beta 1-> 3GalNAc-R， - 另一种是具有多聚乳糖胺骨架的单唾液酸化的三糖基聚糖： - Fucα1 - ＆amp; alpha 2-> 3Gal beta 1-> 4GlcNAc beta 1-> 3Gal beta 1-> - - Fuc alpha l Fuc alpha l - ＆darr＆＆darr＆ - 3 3 - 4GlcNAc beta 1-> 3Gal beta 1-> 4GlcNAc beta 1 - ＆darr＆ 6-Galβ1-> 3GalNAc-R - 其中R = H，OH，另一种糖或糖苷配基如氨基酸，肽或多肽。本文定义的O-聚糖可用于抑制P-选择素介导的炎症。

3. 发明授权

US06667036B2 Methods of treatment using antibodies to P-selectin glycoprotein ligand 失效
标题翻译：使用P-选择蛋白糖蛋白配体的抗体治疗方法
公开(公告)号：US06667036B2
公开(公告)日：2003-12-23
申请号：US10299917
申请日：2002-11-18
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K39395
CPC分类号： C07K16/2896 , A61K38/00 , A61K2039/505 , C07K14/70564 , C07K14/70596 , C07K16/2854
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [3H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells, and to treat various conditions involving leukocyte binding via P-selectin glycoprotein ligand.
摘要翻译：已经证明P-选择蛋白主要结合嗜中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P的亲和层析 - 选择该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂，并且用于治疗涉及白细胞结合的各种病症 P-选择素糖蛋白配体。

4. 发明授权

US06309639B1 Method for inhibiting an inflammatory response using antibodies to P-selectin glycoprotein ligand 失效
标题翻译：使用P-选择蛋白糖蛋白配体的抗体抑制炎症反应的方法
公开(公告)号：US06309639B1
公开(公告)日：2001-10-30
申请号：US09635297
申请日：2000-08-09
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K39395
CPC分类号： C07K16/2896 , A61K38/00 , A61K2039/505 , C07K14/70564 , C07K14/70596 , C07K16/2854
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [3H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
摘要翻译：已经证明P-选择蛋白主要结合中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P-选择素的亲和层析进行评估时。该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂。

5. 发明授权

US06177547B1 Antibodies to P-selectin glycoprotein ligand 失效
标题翻译： P-选择素糖蛋白配体的抗体
公开(公告)号：US06177547B1
公开(公告)日：2001-01-23
申请号：US09207375
申请日：1998-12-08
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： C07K1628
CPC分类号： C07K16/2896 , A61K38/00 , A61K2039/505 , C07K14/705 , C07K14/70564 , C07K14/70596 , C07K16/2854
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [3H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
摘要翻译：已经证明P-选择蛋白主要结合中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P-选择素的亲和层析进行评估时。该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂。

6. 发明授权

US5880091A Glycoprotein ligand for P-selectin and methods of use thereof 失效
公开(公告)号：US5880091A
公开(公告)日：1999-03-09
申请号：US473253
申请日：1995-06-07
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K38/00 , C07K14/705 , C07K16/28 , A61K38/15 , C07H5/04
CPC分类号： C07K16/2896 , C07K14/705 , C07K14/70564 , C07K14/70596 , C07K16/2854 , A61K2039/505 , A61K38/00
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of �.sup.3 H!glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.

7. 发明授权

US06506382B2 Method for inhibiting reperfusion injury using antibodies to P-selectin glycoprotein ligand 失效
标题翻译：使用P-选择蛋白糖蛋白配体的抗体抑制再灌注损伤的方法
公开(公告)号：US06506382B2
公开(公告)日：2003-01-14
申请号：US10039729
申请日：2001-10-29
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K39395
CPC分类号： C07K16/2896 , A61K38/00 , A61K2039/505 , C07K14/70564 , C07K14/70596 , C07K16/2854
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [3H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
摘要翻译：已经证明P-选择蛋白主要结合中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P-选择素的亲和层析进行评估时。该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂。

8. 发明授权

US5464778A Glycoprotein ligand for P-selectin and methods of use thereof 失效
标题翻译： P-选择蛋白的糖蛋白配体及其使用方法
公开(公告)号：US5464778A
公开(公告)日：1995-11-07
申请号：US278551
申请日：1994-07-21
申请人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
发明人： Richard D. Cummings , Kevin L. Moore , Rodger P. McEver
IPC分类号： A61K38/00 , C07K14/705 , C07K16/28 , G01N33/564
CPC分类号： C07K16/2896 , C07K14/705 , C07K14/70564 , C07K14/70596 , C07K16/2854 , A61K2039/505 , A61K38/00
摘要： P-selectin has been demonstrated to bind primarily to a single major glycoprotein ligand on neutrophils and HL-60 cells, when assessed by blotting assays and by affinity chromatography of [.sup.3 H]glucosamine-labeled HL-60 cell extracts on immobilized P-selectin. This molecule was characterized and distinguished from other well-characterized neutrophil membrane proteins with similar apparent molecular mass. The purified ligand, or fragments thereof (including both the carbohydrate and protein components), or antibodies to the ligand, or fragments thereof, can be used as inhibitors of binding of P-selectin to cells.
摘要翻译：已经证明P-选择蛋白主要结合中性粒细胞和HL-60细胞上的单个主要糖蛋白配体，当通过印迹测定和通过[3 H]葡糖胺标记的HL-60细胞提取物对固定化P-选择素的亲和层析进行评估时。该分子的特征与其他具有相似表观分子量的其他具有良好表征的嗜中性粒细胞膜蛋白区别。纯化的配体或其片段（包括碳水化合物和蛋白质组分）或配体的抗体或其片段可用作P-选择素与细胞结合的抑制剂。

9. 发明授权

US06605455B1 Tyrosylprotein sulfotransferases and methods of use thereof 失效
标题翻译：酪氨酸蛋白磺酸转移酶及其使用方法
公开(公告)号：US06605455B1
公开(公告)日：2003-08-12
申请号：US09785343
申请日：2001-02-16
申请人： Kevin L. Moore
发明人： Kevin L. Moore
IPC分类号： C12N910
CPC分类号： C12N9/13 , C07K14/70596 , C07K16/40 , C12Q1/48 , G01N33/573
摘要： Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosyiprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. The enzymes can be used to sulfate peptides or proteins requiring sulfation.
摘要翻译：描述了酪氨酰蛋白质磺酰转移酶和编码酪氨酸蛋白磺酸转移酶的核酸。已经在人，小鼠和秀丽隐杆线虫中鉴定了酶和编码所述酶的核酸的双重同位素。多肽和多核苷酸表现出广泛的同源性。多核苷酸可用于转化或转染宿主细胞以产生基本上纯的形式的酶，或用于在表达系统内产生的蛋白质或肽的翻译后酪氨酸硫酸化的表达系统中。酶可用于需要硫酸化的硫酸肽或蛋白质。

10. 发明授权

US06207414B1 Tyrosylprotein sulfotransferases and methods of use thereof 失效
公开(公告)号：US06207414B1
公开(公告)日：2001-03-27
申请号：US09374824
申请日：1999-08-13
申请人： Kevin L. Moore
发明人： Kevin L. Moore
IPC分类号： C12P2106
CPC分类号： C07K14/70596 , C07K16/40 , C12N9/13 , C12Q1/48 , G01N33/573
摘要： Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosylprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. The enzymes can be used to sulfate peptides or proteins requiring sulfation.

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