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    • 7. 发明申请
    • Droplet Actuator Devices and Methods for Immunoassays and Washing
    • 滴液致动器装置和免疫测定和洗涤方法
    • US20090263834A1
    • 2009-10-22
    • US12393534
    • 2009-02-26
    • Ramakrishna SistaMichael G. PollackVamsee K. PamulaVijay Srinivasan
    • Ramakrishna SistaMichael G. PollackVamsee K. PamulaVijay Srinivasan
    • G01N33/542
    • G01N33/54333
    • Droplet actuator devices and methods for immunoassays and washing are provided. According to one embodiment, a method of providing a droplet in contact with a surface of a super paramagnetic bead with a reduced concentration of a substance is provided and includes: (a) providing a super paramagnetic bead in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets. The set of droplets includes: (i) a droplet in contact with the super paramagnetic bead having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the super paramagnetic bead.
    • 提供液滴致动器装置和免疫测定和洗涤方法。 根据一个实施方案,提供了一种提供与物质浓度降低的超顺磁珠的表面接触的液滴的方法,包括:(a)提供与包含起始浓度的液滴接触的超顺磁珠 并开始物质的起始体积; (b)进行一个或多个液滴操作以将洗涤液滴与步骤(a)中提供的液滴合并以产生组合液滴; 和(c)进行一个或多个液滴操作以分开组合液滴以产生一组液滴。 一组液滴包括:(i)与超顺磁珠接触的液滴,其物质相对于起始浓度降低; 和(ii)从超顺磁珠分离的液滴。