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    • 2. 发明授权
    • Protein profiling of hyper acidic plants and high protein extraction compositions thereof
    • 高酸性植物的蛋白质分析及其高蛋白质提取组合物
    • US06893667B2
    • 2005-05-17
    • US10104062
    • 2002-03-22
    • Rajender Singh SangwanNeelam Singh SangwanBali Ram TyagiAvdhesh Kumar SrivastavaUsha Yadav
    • Rajender Singh SangwanNeelam Singh SangwanBali Ram TyagiAvdhesh Kumar SrivastavaUsha Yadav
    • C12Q1/28C12Q1/44C12Q1/52A61K35/78A01N37/18
    • C12Q1/52C12Q1/28C12Q1/44G01N2550/00
    • The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.
    • 本发明涉及一种用于从植物香味天竺葵(天竺葵属)的高酸性地上部分分析蛋白质提取物的方法,所述方法包括以下步骤:选择有效的提取介质,用0.2M碳酸钠提取百分比提取物 选择有效培养基以确定所述提取物中的多肽模式,多肽模式培养基由碳酸钠组成,范围在0.2至0.4M之间,0.2M碳酸钠与50%二甲基亚砜组成,选择有效培养基以确定酶多分子形式 在所述提取物中,用酶多分子形式的培养基由pH7.5的0.2M Tris-HCl缓冲液和任选的50%二甲基亚砜组成; 碳酸钠的浓度范围为0.2至0.4M,任选的50%的二甲基亚砜,并且使用生物化学和生物物理技术,包括凝胶电泳,分光光度法,离心,所述方法中的蛋白质估计,以确定所述蛋白质的定量,定性,结构和功能方面 提取物及其提取组合物。