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1. 发明授权

US5262320A Cell-culturing apparatus and method employing a macroporous support 失效
标题翻译：细胞培养装置和使用大孔支持物的方法
公开(公告)号：US5262320A
公开(公告)日：1993-11-16
申请号：US921920
申请日：1992-07-29
申请人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
发明人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
IPC分类号： C12M1/04 , C12M1/40 , C12M3/06 , C12N5/00 , A01N1/02 , C12M3/00
CPC分类号： C12M25/18 , C12M23/24 , C12M29/04 , C12M29/06 , C12N5/0068
摘要： An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.
摘要翻译：公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量包装床或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。

2. 发明授权

US5510262A Cell-culturing apparatus and method employing a macroporous support 失效
标题翻译：细胞培养装置和使用大孔支持物的方法
公开(公告)号：US5510262A
公开(公告)日：1996-04-23
申请号：US153204
申请日：1993-11-16
申请人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
发明人： Gregory Stephanopoulos , Rahul Singhvi , Seujeung Park , Maria Flytzani-Stephanopoulos , Mark A. Applegate
IPC分类号： C12M1/04 , C12M1/40 , C12M3/06 , C12N5/00 , C12M3/00
CPC分类号： C12M25/18 , C12M23/24 , C12M29/04 , C12M29/06 , C12N5/0068
摘要： An apparatus and method are disclosed for culturing cells by flowing a medium through a packed bed of biocompatible macroporous ceramic particles disposed in a cell-culture reactor. In one embodiment, oxygen and other nutrients in the medium are transported significantly by convective flow through pores of the biocompatible macroporous ceramic particles to cells disposed within the pores. Oxygen can be introduced to the cell-culture reactor by sparging oxygen gas into the cell-culture reactor. Oxygen content in the medium can also be increased by increasing the solubility of oxygen in the medium, such as by adding perfluorocarbon or other oxygen carriers to the medium. The oxygen capacity of the reactor can be further increased by sparging oxygen-containing gas into the medium at a plurality of points in the packed bed, or by introducing oxygen gas to the medium through a solid-phase oxygen gas-permeable membrane disposed in the packed bed. Alternatively, an oxygen-containing gas can be directed through an oxygen-permeable conduit disposed within at least one passage of a biocompatible macroporous support.
摘要翻译：公开了一种用于通过使介质流过细胞培养反应器中的生物相容性大孔陶瓷颗粒的填充床来培养细胞的装置和方法。在一个实施方案中，培养基中的氧和其他营养物质通过生物相容性大孔陶瓷颗粒的孔对流流动被显着转运到设置在孔内的细胞中。可以通过将氧气喷射到细胞培养反应器中来将氧气引入细胞培养反应器。培养基中的氧含量也可以通过增加介质中氧的溶解度来增加，例如通过向培养基中加入全氟化碳或其它氧载体。通过在填充床中的多个点处将含氧气体喷射到介质中，或者通过将氧气引入到介质中，通过设置在所述填充床中的固相氧气可渗透膜，可以进一步增加反应器的氧气容量包装床或者，可以将含氧气体引导通过设置在生物相容性大孔载体的至少一个通道内的透氧导管。

3. 发明授权

US07993905B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07993905B2
公开(公告)日：2011-08-09
申请号：US12166287
申请日：2008-07-01
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
IPC分类号： C12N1/00 , C12N11/00 , C12N11/08
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

4. 发明授权

US07410791B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07410791B2
公开(公告)日：2008-08-12
申请号：US11331849
申请日：2006-01-13
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory Stephanopoulos
IPC分类号： C12N11/00 , C12N11/08 , C12N1/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

5. 发明申请

US20060210595A1 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
公开(公告)号：US20060210595A1
公开(公告)日：2006-09-21
申请号：US11331849
申请日：2006-01-13
申请人： Rahul Singhvi , Amit Kumar , George Whitesides , Donald Ingber , Gabriel Lopez , Daniel Wang , Gregory Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George Whitesides , Donald Ingber , Gabriel Lopez , Daniel Wang , Gregory Stephanopoulos
IPC分类号： A61F2/06 , A61F13/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

6. 发明申请

US20090011493A1 DEVICE CONTAINING CYTOPHILIC ISLANDS THAT ADHERE CELLS SEPARATED BY CYTOPHOBIC REGIONS 失效
标题翻译：含有CYPOPHOBIC区域分离细胞的胞质岛的装置
公开(公告)号：US20090011493A1
公开(公告)日：2009-01-08
申请号：US12166287
申请日：2008-07-01
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
IPC分类号： C12M1/00
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

7. 发明申请

US20060251722A1 Multi-component vitamin and mineral supplement for the optimal absorption of components 审中-公开
标题翻译：多组分维生素和矿物质补充剂可以最佳地吸收组分
公开(公告)号：US20060251722A1
公开(公告)日：2006-11-09
申请号：US11120767
申请日：2005-05-03
申请人： Stephen Bandak , Rahul Singhvi
发明人： Stephen Bandak , Rahul Singhvi
IPC分类号： A61K9/24
CPC分类号： A61K9/5073 , A61K9/2081 , A61K9/209
摘要： The present invention relates to compositions and methods for administration of vitamin-mineral supplements, including those given to women prenatally. A multi-component vitamin delivery system is described comprising a first layer able to release a first set of components at a first location in the gastrointestinal tract, the first set of components includes iron in a form absorbable at the first location and a second layer able to release a second set of components at a second location in the gastrointestinal tract, the second location being further downstream in the gastrointestinal tract than the first location. The second set of components includes at least one material that reduces or inhibits the absorption of iron or whose absorption is inhibited by iron. Components are incorporated into the different layers of the delivery system to minimize the potential for interactions that may impair absorption and maximize conditions for optimal absorption.
摘要翻译：本发明涉及用于施用维生素矿物质补充剂的组合物和方法，包括给予妇女前列腺素的补充剂。描述了多组分维生素递送系统，其包括能够在胃肠道中的第一位置释放第一组成分的第一层，第一组成分包括在第一位置可吸收的形式的铁和第二层，以在胃肠道的第二位置释放第二组成分，第二位置在第一位置处在胃肠道的下游。第二组成分包括至少一种减少或抑制铁的吸收或其吸收被铁抑制的材料。将组分并入到递送系统的不同层中以最小化可能损害吸收并最大化最佳吸收条件的相互作用的可能性。

8. 发明授权

US07067306B2 Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US07067306B2
公开(公告)日：2006-06-27
申请号：US10032280
申请日：2001-12-21
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
IPC分类号： C12Q1/02 , C12Q1/04 , C12N11/02 , C12N11/08
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).
摘要翻译：本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。

9. 发明授权

US06368838B1 Adhering cells to cytophilic islands separated by cytophobic regions to form patterns and manipulate cells 有权
标题翻译：将细胞粘附到由细胞疏植区域分离的细胞色素岛以形成模式并操纵细胞
公开(公告)号：US06368838B1
公开(公告)日：2002-04-09
申请号：US09373334
申请日：1999-08-12
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
IPC分类号： C12N1102
CPC分类号： C12N1/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C1/00206 , B81C2201/034 , B82Y5/00 , B82Y30/00 , C12N5/0068 , C12N5/067 , C12N2535/10 , C12Q1/02 , C40B60/14
摘要： Methods and a device are provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere. The cytophobic regions can be wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. Further, the islands or the regions or both may be formed of a self-assembled monolayer (SAM). The methods are for forming a patterned surface for a population of cells and for selectively manipulating individual cells formed on the surface of the plate of the device. Furthermore, the device is used in immobilization of cells at a surface and for controlling shape of the cells.
摘要翻译：提供了用于将细胞粘附在特定和预定位置的方法和装置。该装置包括限定表面的板和附着由细胞不粘附细胞渗透区域分离的细胞的多个细胞色素岛。细胞疏植区域可以足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。此外，岛或区域或两者可以由自组装单层（SAM）形成。这些方法用于形成用于细胞群的图案化表面并且用于选择性地操纵形成在装置的板的表面上的单个细胞。此外，该装置用于在表面固定细胞并用于控制细胞的形状。

10. 发明授权

US5976826A Device containing cytophilic islands that adhere cells separated by cytophobic regions 失效
标题翻译：包含细胞吸附岛的装置，其附着由细胞疏植区域分离的细胞
公开(公告)号：US5976826A
公开(公告)日：1999-11-02
申请号：US951886
申请日：1997-10-16
申请人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
发明人： Rahul Singhvi , Amit Kumar , George M. Whitesides , Donald E. Ingber , Gabriel P. Lopez , Daniel I. C. Wang , Gregory N. Stephanopoulos
IPC分类号： B01J19/00 , B81C1/00 , C12N5/00 , C12N5/02 , C12N5/071 , C40B60/14 , C12Q1/02 , C12N11/02 , C12N11/08 , C12Q1/04
CPC分类号： C12N5/067 , B01J19/0046 , B81C1/00206 , B82Y30/00 , B82Y5/00 , C12N5/0068 , B01J2219/00382 , B01J2219/00385 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/0072 , B81B2201/0214 , B81C2201/034 , C12N2535/10 , C40B60/14
摘要： A device is provided for adhering cells in a specific and predetermined position. The device comprises a plate defining a surface and a plurality of cytophilic islands that adhere cells which are isolated by cytophobic regions to which cells do not adhere and further is contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). Further, the cytophobic regions are wide enough such that less than 10 percent of the cells adhered to the cytophilic islands are allowed to form bridges across the cytophobic regions and contact each other. The device is used in a method for culturing cells on a surface or in a medium and also for performing cytometry. Furthermore, the device is used in immobilization of cells at a surface and for controlling the shape of a cell.
摘要翻译：提供了用于将细胞粘附在特定和预定位置的装置。该装置包括限定表面的板和多个细胞亲和岛，所述细胞亲和岛附着细胞，细胞被细胞悬浮区域分离，细胞不粘附并进一步与细胞色素岛邻接。岛或区域或两者可以由自组装单层（SAM）形成。此外，细胞疏气区域足够宽，使得少于10％的细胞粘附到细胞色素岛被允许跨细胞疏漏区域形成桥接并彼此接触。该装置用于在表面或培养基中培养细胞并用于进行细胞计数的方法。此外，该装置用于在表面固定细胞并用于控制细胞的形状。

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