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    • 4. 发明申请
    • HETEROGENOUS ENZYMATIC CATALYST, PREPARATION METHOD, AND USE
    • 异构酶催化剂,制备方法和使用
    • US20120196337A1
    • 2012-08-02
    • US13380929
    • 2010-07-05
    • Nicolas BrunAnnick Babeau-GarciaClément SanchezRénal Backov
    • Nicolas BrunAnnick Babeau-GarciaClément SanchezRénal Backov
    • C12P7/64C12N11/02C12N11/14
    • C12N9/20C11C1/045C11C3/003C11C3/10C12N11/14
    • The invention relates to a heterogenous enzymatic catalyst that takes the form of a cellular monolith consisting of a silica or organically modified silica matrix, said monolith including macropores, mesopores, and micropores, said pores being interconnected, and wherein the inner surface of the macropores is functionalized by a coupling agent selected from among silanes, said inner surface moreover having an unpurified enzyme attached thereon by means of a covalent or electrostatic bond. The invention also relates to the method for preparing said catalyst, said method comprising: a first step for preparing a solid silica impression that takes the form of a cellular monolith such as defined above; a second step for functionalizing the inner surface of the macropores via a coupling agent, selected from among silanes, by vacuum-soaking the cellular monolith by dissolving the coupling agent in an organic solvent; and a third step for vacuum-soaking the thus-functionalized monolith by means of an aqueous solution or aqueous dispersion of at least one unpurified enzyme. Finally, the invention relates to the use of such catalyst to carry out catalyzed chemical reactions.
    • 本发明涉及一种异源酶催化剂,其采用由二氧化硅或有机改性的二氧化硅基质组成的细胞整体的形式,所述整料包括大孔,介孔和微孔,所述孔相互连接,并且其中大孔的内表面是 通过选自硅烷的偶联剂官能化,所述内表面还具有通过共价或静电键连接在其上的未纯化的酶。 本发明还涉及制备所述催化剂的方法,所述方法包括:制备呈上述定义的细胞整体形式的固体二氧化硅印模的第一步骤; 通过将偶联剂溶解在有机溶剂中,通过将选自硅烷的偶联剂通过真空浸泡多孔整体而使大孔的内表面官能化的第二步骤; 以及通过至少一种未纯化的酶的水溶液或水分散体将如此官能化的整料真空浸泡的第三步骤。 最后,本发明涉及这种催化剂用于催化化学反应的用途。