专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10745686B2 Method for separating DNA by size 有权
公开(公告)号：US10745686B2
公开(公告)日：2020-08-18
申请号：US14764045
申请日：2014-02-07
申请人： QIAGEN GmbH
发明人： Tanya Sperling , Nicola Scholle , Thorsten Singer , Margit Hiesinger
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869 , G01N33/50
摘要： The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.

2. 发明授权

US10711265B2 Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach 有权
公开(公告)号：US10711265B2
公开(公告)日：2020-07-14
申请号：US15576353
申请日：2016-06-01
申请人： Qiagen GmbH
发明人： Thorsten Singer , Sarah Fakih
IPC分类号： G01N27/447 , C12N15/10 , B01D15/20 , B01D15/42 , B01D57/02
摘要： Provided is inter alia to an electrophoresis assisted method for purifying a target nucleic acid from a nucleic acid containing sample, comprising (a) binding the target nucleic acid to a solid phase; (b) placing the solid phase with the bound target nucleic acid into a loading chamber of a device, wherein the device comprises a passage which comprises the loading chamber, optionally a liquid permeable separation matrix adjacent to the loading chamber, and a liquid permeable collection matrix and wherein the solid phase with the bound target nucleic acid is present in the loading chamber in a liquid medium comprising at least one water-miscible organic solvent and wherein the target nucleic acid remains bound to the solid phase in said liquid medium; (c) generating an electric field between a cathode and an anode and using a running solution that conducts the electric current, wherein the running solution dilutes the liquid medium comprised in the loading chamber resulting in elution of the bound target nucleic acid, and wherein the eluted target nucleic acid migrates according to its charge in the electric field and is retained by the collection matrix; (d) collecting the purified target nucleic acid. The method is particularly suitable for isolating RNA. The liquid medium delays elution of the RNA from the solid phase, thereby preventing a degradation of the RNA by e.g. RNases.

3. 发明授权

US10794859B2 Electrophoresis assisted method and device for purifying a charged target molecule from a sample 有权
公开(公告)号：US10794859B2
公开(公告)日：2020-10-06
申请号：US15576310
申请日：2016-06-01
申请人： QIAGEN GMBH
发明人： Thorsten Singer , Sarah Fakih , Sabine Kuchler , Corinna Küppers , Lother Breitkopf , Maximilian Focke , Nicole Seip
IPC分类号： G01N27/447 , C12N15/10
摘要： The invention inter alia pertains to an electrophoresis assisted method for purifying at least one charged target molecule, preferably a nucleic acid, from a sample. Moreover, a device for use in a method for purifying a charged target molecule by electrophoresis is provided.

4. 发明申请

US20180202968A1 Electrophoresis assisted method and device for purifying a charged target molecule from a sample 审中-公开
公开(公告)号：US20180202968A1
公开(公告)日：2018-07-19
申请号：US15576310
申请日：2016-06-01
申请人： QIAGEN GMBH
发明人： Thorsten Singer , Sarah Fakih , Sabine Kuchler , Corinna Küppers , Lother Breitkopf , Maximilian Focke , Nicole Seip
IPC分类号： G01N27/447 , C12N15/10
CPC分类号： G01N27/44739 , C12N15/101 , G01N27/44747
摘要： The invention inter alia pertains to an electrophoresis assisted method for purifying at least one charged target molecule, preferably a nucleic acid, from a sample. Moreover, a device for use in a method for purifying a charged target molecule by electrophoresis is provided.

5. 发明申请

US20180148710A1 Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach 审中-公开
公开(公告)号：US20180148710A1
公开(公告)日：2018-05-31
申请号：US15576353
申请日：2016-06-01
申请人： Qiagen GmbH
发明人： Thorsten Singer , Sarah Fakih
IPC分类号： C12N15/10 , B01D15/20 , B01D15/42 , B01D57/02
摘要： Provided is inter alia to an electrophoresis assisted method for purifying a target nucleic acid from a nucleic acid containing sample, comprising (a) binding the target nucleic acid to a solid phase; (b) placing the solid phase with the bound target nucleic acid into a loading chamber of a device, wherein the device comprises a passage which comprises the loading chamber, optionally a liquid permeable separation matrix adjacent to the loading chamber, and a liquid permeable collection matrix and wherein the solid phase with the bound target nucleic acid is present in the loading chamber in a liquid medium comprising at least one water-miscible organic solvent and wherein the target nucleic acid remains bound to the solid phase in said liquid medium; (c) generating an electric field between a cathode and an anode and using a running solution that conducts the electric current, wherein the running solution dilutes the liquid medium comprised in the loading chamber resulting in elution of the bound target nucleic acid, and wherein the eluted target nucleic acid migrates according to its charge in the electric field and is retained by the collection matrix; (d) collecting the purified target nucleic acid. The method is particularly suitable for isolating RNA. The liquid medium delays elution of the RNA from the solid phase, thereby preventing a degradation of the RNA by e.g. RNases.

6. 发明授权

US10655122B2 Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample 有权
公开(公告)号：US10655122B2
公开(公告)日：2020-05-19
申请号：US15568098
申请日：2015-04-20
申请人： QIAGEN GmbH
发明人： Johann Kubicek , Thorsten Singer , Antje-Katrin Sander , Eva Hänssler , Dominic O'Neil
IPC分类号： C12Q1/6806 , C12N1/06 , C12N15/10 , C12N9/22
摘要： The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

7. 发明申请

US20180142231A1 Method, Lysis Solution and Kit for Selectively Depleting Animal Nucleic Acids in a Sample 审中-公开
公开(公告)号：US20180142231A1
公开(公告)日：2018-05-24
申请号：US15568098
申请日：2015-04-20
申请人： QIAGEN GmbH
发明人： Johann Kubicek , Thorsten Singer , Antje-Katrin Sander
IPC分类号： C12N15/10 , C12N9/22 , C12Q1/6806
CPC分类号： C12N15/1003 , C12N9/22 , C12Q1/6806 , C12Q2521/301 , C12Q2521/537 , C12Q2523/113
摘要： The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式