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    • 3. 发明授权
    • Polyionic insoluble hydrogels comprising xanthan and chitosan
    • 包含黄原胶和壳聚糖的聚阴离子不溶性水凝胶
    • US5620706A
    • 1997-04-15
    • US416025
    • 1995-04-03
    • Severian DumitriuEsteban ChornetPierre Vidal
    • Severian DumitriuEsteban ChornetPierre Vidal
    • A61K9/00A61K9/16A61K38/46A61K47/36C12N11/04A61K9/10A61K9/14
    • C12N11/04A61K38/14A61K38/465A61K38/47A61K38/48A61K45/06A61K47/36A61K9/0014A61K9/1652Y10S514/944
    • A method for the preparation of insoluble hydrogels from the complexation of polycations and xanthan is reported. Stable hydrogels capable of retaining between 65 and 95 % weight water were prepared particularly with chitosan and xanthan. The water retention and properties of the hydrogels were studied as a function of the degree of acetylation of chitosan and the ratio chitosan/xanthan used in the preparation of the gel. The chitosan-xanthan complex was used to immobilize biological material. Hydrogels containing enzymes (for example endo-1,4-.beta.-xylanase and protease) either as single enzymes or as a binary system have been prepared. Immobilization varied between 85 and 98 % . The immobilized xylanase activity was significantly greater with respect to the free enzyme while the binary enzyme system promoted protease activity. Other hydrogels prepared with polybasic drugs complexed to xanthan with or without chitosan have been prepared. These complexes slowly dissociate in acidic media and provide for sustained release of compounds in near neutral pHs. Gels containing chitosan are stable in all physiological pHs and the immobilized molecules are released therefrom by diffusion.
    • 报道了从聚阳离子和黄原胶的络合制备不溶性水凝胶的方法。 特别是用壳聚糖和黄原胶制备能够保持65至95重量%水的稳定的水凝胶。 研究了水凝胶的保水性和性能,作为凝胶制备中壳聚糖的乙酰化程度和壳聚糖/黄原胶比例的函数。 壳聚糖 - 黄原胶配合物用于固定生物材料。 已经制备了含有酶(例如内-4,4-β-木聚糖酶和蛋白酶)的单独酶或二元体系的水凝胶。 固定在85%和98%之间变化。 相对于游离酶,固定化的木聚糖酶活性显着更大,而二元酶系统促进蛋白酶活性。 已经制备了与具有或不具有壳聚糖的黄原胶复合的多元药物制备的其它水凝胶。 这些复合物在酸性介质中缓慢解离并提供化合物在近中性pH值的持续释放。 含有壳聚糖的凝胶在所有生理pH中都是稳定的,并且通过扩散将固定的分子从其中释放出来。