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1. 发明申请

US20070028326A1 Method for producing multiple unsaturated fatty acids in plants 有权
标题翻译：在植物中生产多种不饱和脂肪酸的方法
公开(公告)号：US20070028326A1
公开(公告)日：2007-02-01
申请号：US10511621
申请日：2003-04-25
申请人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
发明人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
IPC分类号： A01H1/00 , C12N9/04 , C12N15/82 , C12N5/04
CPC分类号： C12P7/6427 , C12N9/0083 , C12N15/8247 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a Δ6-desaturase activity, Δ6-elongase activity or Δ5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
摘要翻译：本发明涉及一种生产脂肪酸酯的方法，所述脂肪酸酯包含具有至少三个双键的不饱和脂肪酸，以及游离不饱和脂肪酸，所述不饱和脂肪酸的含量为至少1重量％通过表达至少一个编码具有Δ6-去饱和酶活性的多肽的核酸序列和至少一个编码具有Δ6-延长酶活性的多肽的核酸序列的植物。有利地，如果合适，这些核酸序列可以与编码具有Δ5-去饱和酶活性的多肽的第三核酸序列一起在转基因植物中表达。本发明还涉及定义的编码具有Δ6-去饱和酶活性的多肽，Δ6-延长酶活性或选自一组核酸序列的Δ5-去饱和酶活性的定义的核酸序列的用途，和/或使用核酸构建体包含上述核酸序列。

2. 发明授权

US07893320B2 Method for producing multiple unsaturated fatty acids in plants 有权
标题翻译：在植物中生产多种不饱和脂肪酸的方法
公开(公告)号：US07893320B2
公开(公告)日：2011-02-22
申请号：US10511621
申请日：2003-04-25
申请人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
发明人： Petra Cirpus , Andreas Renz , Jens Lerchl , Anne-Marie Kuijpers
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12P7/6427 , C12N9/0083 , C12N15/8247 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a method for the production of fatty acid esters which comprise unsaturated fatty acids with at least three double bonds, and to free unsaturated fatty acids with a content of at least 1% by weight based on the total fatty acids present in the plants, by expressing at least one nucleic acid sequence which encodes a polypeptide with Δ6-desaturase activity and at least one nucleic acid sequence which encodes a polypeptide with Δ6-elongase activity. Advantageously, these nucleic acid sequences can, if appropriate, be expressed in the transgenic plant together with a third nucleic acid sequence which encodes a polypeptide with Δ5-desaturase activity. The invention furthermore relates to the use of defined nucleic acid sequences which encode polypeptides with a Δ6-desaturase activity, Δ6-elongase activity or Δ5-desaturase activity selected from a group of nucleic acid sequences, and/or to the use of nucleic acid constructs comprising the abovementioned nucleic acid sequences.
摘要翻译：本发明涉及一种生产脂肪酸酯的方法，所述脂肪酸酯包含具有至少三个双键的不饱和脂肪酸，以及游离不饱和脂肪酸，所述不饱和脂肪酸的含量为至少1重量％通过表达编码具有＆Dgr.6-去饱和酶活性的多肽的至少一个核酸序列和至少一个编码具有＆Dgr.6-延伸酶活性的多肽的核酸序列的植物。有利的是，如果合适，这些核酸序列可以与编码具有＆Dgr。5-去饱和酶活性的多肽的第三核酸序列一起在转基因植物中表达。本发明进一步涉及定义的核酸序列的用途，所述核酸序列编码具有选自一组核酸序列的＆Dgr .6-去饱和酶活性和/或Dgr。5-去饱和酶活性的多肽，和/或使用包含上述核酸序列的核酸构建体。

3. 发明授权

US10190131B2 Method for producing polyunsaturated fatty acids 有权
公开(公告)号：US10190131B2
公开(公告)日：2019-01-29
申请号：US12280090
申请日：2007-02-21
申请人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： A61K31/20 , C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

4. 发明授权

US08278506B2 Sugar and lipid metabolism regulators in plants II 失效
标题翻译：植物中的糖和脂质代谢调节剂II
公开(公告)号：US08278506B2
公开(公告)日：2012-10-02
申请号：US13103572
申请日：2011-05-09
申请人： Volker Mittendorf , Heiko A. Haertel , Petra Cirpus
发明人： Volker Mittendorf , Heiko A. Haertel , Petra Cirpus
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8247
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供了来自拟南芥的脂质代谢蛋白（LMP）和编码核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

5. 发明申请

US20090158462A1 Method for Producing Polyunsaturated C20 and C22 Fatty Acids with at Least Four Double Bonds in Transgenic Plants 有权
标题翻译：在转基因植物中用最少四个双键生产多不饱和C20和C22脂肪酸的方法
公开(公告)号：US20090158462A1
公开(公告)日：2009-06-18
申请号：US11886857
申请日：2006-03-21
申请人： Petra Cirpus , Jörg Bauer
发明人： Petra Cirpus , Jörg Bauer
IPC分类号： C12N15/11 , C11B1/00 , A23D9/00 , A61K47/00 , C07H21/04 , C07K14/00 , C12N15/63
CPC分类号： C12P7/6472 , C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427
摘要： The present invention relates to a process for the production of polyunsaturated fatty acids in transgenic plants, by introducing, into the plant, the nucleic acids which code for polypeptides with Δ6-desaturase, Δ6-elongase, a Δ5-desaturase, Δ5-elongase, Δ4-desaturase, Δ12-desaturase and/or ω3-desaturase activity. These desaturases and elongases are advantageously derived from Phytophthora sojae. The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences according to the invention, vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic plants comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.A further part of the invention relates to fatty acid compositions produced by the process according to the invention and to their use.
摘要翻译：本发明涉及在转基因植物中生产多不饱和脂肪酸的方法，通过向植物中引入编码具有Δ6-去饱和酶，Δ6-延伸酶，Δ5-去饱和酶，Δ5-延伸酶， Δ4-去饱和酶，Δ12-去饱和酶和/或ω3-去饱和酶活性。这些去饱和酶和延长酶有利地来源于疫霉属（Phytophthora sojae）。本发明还涉及包含根据本发明的核酸序列的核酸序列，核酸构建体，载体和生物体，包含核酸序列和/或核酸构建体的载体和转基因植物包含上述核酸序列，核酸构建体和/或载体。本发明的另一部分涉及通过根据本发明的方法生产的脂肪酸组合物及其用途。

6. 发明申请

US20090094707A1 Method for Producing Polyunsaturated Long-Chain Fatty Acids in Transgenic Organisms 有权
标题翻译：生物多不饱和长链脂肪酸在转基因生物中的应用
公开(公告)号：US20090094707A1
公开(公告)日：2009-04-09
申请号：US11793854
申请日：2005-12-20
申请人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
发明人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
IPC分类号： A01K67/027 , C12N15/11 , C12N9/12 , C12N15/00 , C11B1/00 , C12P7/64 , C12N1/21 , A01H5/00
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity am introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及在生物体中生产多不饱和脂肪酸的方法，根据该方法将编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽的核酸引入生物体中。有利地，所述核酸序列可以任选与编码脂肪酸或脂质代谢多肽的其它核酸序列一起在转基因生物体中表达。本发明还涉及本发明的核酸序列，含有本发明核酸序列的核酸构建体，含有本发明核酸序列和/或核酸构建体的载体，以及含有所述核酸序列，核酸构建体和/ 或向量。本发明还涉及根据本发明方法生产的油，脂和/或脂肪酸及其用途。

7. 发明授权

US08835714B2 Sugar and lipid metabolism regulators in plants II 有权
标题翻译：植物中的糖和脂质代谢调节剂II
公开(公告)号：US08835714B2
公开(公告)日：2014-09-16
申请号：US13623212
申请日：2012-09-20
申请人： Volker Mittendorf , Heiko A. Haertel , Petra Cirpus
发明人： Volker Mittendorf , Heiko A. Haertel , Petra Cirpus
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N15/29 , C12N15/82 , C07H21/04 , C07K14/415
CPC分类号： C07K14/415 , C12N15/8247
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供了来自拟南芥的脂质代谢蛋白（LMP）和编码核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

8. 发明授权

US08373024B2 Method for producing unsaturated ω-3-fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US08373024B2
公开(公告)日：2013-02-12
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： A01H5/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明核酸序列的至少一种核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

9. 发明授权

US07964769B2 Sugar and lipid metabolism regulators in plants II 有权
标题翻译：植物中的糖和脂质代谢调节剂II
公开(公告)号：US07964769B2
公开(公告)日：2011-06-21
申请号：US11998203
申请日：2007-11-29
申请人： Volker Mittendorf , Heiko Haertel , Petra Cirpus
发明人： Volker Mittendorf , Heiko Haertel , Petra Cirpus
IPC分类号： A01H5/00 , A01H5/10 , A01H1/00 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8247
摘要： Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation are provided. In particular, lipid metabolism proteins (LMP) and encoding nucleic acids originating from Arabidopsis thaliana are provided. The nucleic acids and proteins are used in methods of producing transgenic plants and modulating levels of seed storage compounds. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.
摘要翻译：提供了与脂质和糖代谢调节相关的分离的核酸和蛋白质。特别地，提供了来自拟南芥的脂质代谢蛋白（LMP）和编码核酸。核酸和蛋白质用于产生转基因植物和调节种子储存化合物水平的方法中。优选地，种子储存化合物是脂质，脂肪酸，淀粉或种子储存蛋白。

10. 发明申请

US20090172837A1 PROCESS FOR PRODUCING ARACHIDONIC ACID AND/OR EICOSAPENTAENOIC ACID IN PLANTS 审中-公开
标题翻译：在植物中生产芳香酸和/或异柠檬酸的方法
公开(公告)号：US20090172837A1
公开(公告)日：2009-07-02
申请号：US12374429
申请日：2007-07-11
申请人： Michael Geiger , Jörg Bauer , Petra Cirpus
发明人： Michael Geiger , Jörg Bauer , Petra Cirpus
IPC分类号： C12N15/82 , C11B1/00
CPC分类号： C12N15/8247 , A23L33/12 , C12N9/0083 , C12N9/1029
摘要： The present invention relates to a process for the production of arachidonic acid (=APA) or eicosapentaenoic acid (=EPA) or arachidonic acid and eicosapentaenoic acid, advantageously in the seed of transgenic plants of the family Brassicaceae with a content of ARA or EPA or ARA and EPA of at least 3% by weight based on the total lipid content of the transgenic plant, by introducing, into the organism, nucleic acids which code polypeptides with Δ6-desaturase, Δ6-elongase and Δ5-desaturase activity, where, as the result of the enzymatic activity of the introduced enzymes, a fatty acid selected from the group consisting of the fatty acids oleic acid [C18:1Δ9], linoleic acid [C18:2Δ9, 12], α-linolenic acid [C18:3Δ6, 9, 12], icosenoic acid (20:1Δ11) and erucic acid [C22:1Δ11] is reduced by at least 10% in comparison with the nontransgenic wild-type plant. Advantageously, further enzymes selected from the group of the enzymes (ω3-desaturases, Δ12-desaturases, Δ6-desaturases, Δ6-elongases, Δ5-desaturases, Δ5-elongases and/or Δ4-desaturases can be introduced into the plants.
摘要翻译：本发明涉及一种生产花生四烯酸（= APA）或二十碳五烯酸（= EPA）或花生四烯酸和二十碳五烯酸的方法，有利地在含有ARA或EPA的甘蓝科植物的转基因植物的种子中 ARA和EPA基于转基因植物的总脂质含量至少为3重量％，通过向生物体引入用Delta6-去饱和酶，Δ6-延长酶和Δ5-去饱和酶活性编码多肽的核酸，其中，作为引入的酶的酶活性的结果，选自脂肪酸油酸[C18：1Delta9]，亚油酸[C18：2Delta12,12]，α-亚麻酸[C18：3Delta6， 9，12]，二十碳烯酸（20：1Delta11）和芥酸[C22：1Delta11]与非转基因野生型植物相比降低至少10％。有利地，可以将植物中的酶（ω3-去饱和酶，Δ12-去饱和酶，Δ6-去饱和酶，Δ6-延长酶，Δ5-去饱和酶，Δ5-延伸酶和/或Delta4-去饱和酶）的其他酶引入。

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