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1. 发明授权

US06541254B1 Glycosylation variants of iduronate 2-sulfatase 有权
标题翻译：糖尿病糖苷酸2-硫酸酯酶的糖基化变体
公开(公告)号：US06541254B1
公开(公告)日：2003-04-01
申请号：US09685844
申请日：2000-10-10
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： C12N510
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
摘要翻译：本发明提供了高度糖基化的糖尿病2-硫酸酯酶，其包含与天然来源例如纯化的伊曲膦酸酯-2-硫酸酯酶相比，具有至少5千道尔顿（kDa）多糖的伊万二酸-2-硫酸酯酶多肽。人肝。本发明还提供了这样一种高度糖基化的阿魏酸-2-硫酸酯酶的酶活性多肽片段或变体。本发明还提供了编码艾杜糖醛酸-2-硫酸酯酶的分离的核酸，以及表达载体，宿主细胞和用于制备本发明的高度糖基化的阿拉伯糖酸-2-硫酸酯酶的方法。在一个实施方案中，本发明涉及一种生产糖基化的阿魏酸-2-硫酸酯酶的方法，其包括培养含有编码酶活性的阿魏酸-2-硫酸酯酶多肽的核酸的宿主细胞，其中宿主细胞将多肽糖基化成比由天然人肝细胞表达的天然异二烯酸2-硫酸酯酶多肽更大程度。

2. 发明授权

US5728381A Glycosylation variants of iduronate 2-sulfatase 失效
公开(公告)号：US5728381A
公开(公告)日：1998-03-17
申请号：US484493
申请日：1995-06-07
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46 , A61K38/47 , C12N9/00
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

3. 发明授权

US06153188A Glycosylation variants of iduronate 2-sulfatase 有权
公开(公告)号：US06153188A
公开(公告)日：2000-11-28
申请号：US249003
申请日：1999-02-12
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

4. 发明授权

US5932211A Glycosylation variants of iduronate 2-sulfatase 失效
标题翻译：糖尿病糖苷酸2-硫酸酯酶的糖基化变体
公开(公告)号：US5932211A
公开(公告)日：1999-08-03
申请号：US345212
申请日：1994-11-28
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present intention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
摘要翻译：本发明提供了高度糖基化的糖尿病2-硫酸酯酶，其包含与天然来源例如纯化的伊曲膦酸酯-2-硫酸酯酶相比，具有至少5千道尔顿（kDa）多糖的伊万二酸-2-硫酸酯酶多肽。人肝。本发明还提供了这样一种高度糖基化的阿魏酸-2-硫酸酯酶的酶活性多肽片段或变体。本发明还提供了编码艾杜糖醛酸-2-硫酸酯酶的分离的核酸，以及表达载体，宿主细胞和用于生产本发明的高度糖基化的糖尿病2-硫酸酯酶的方法。在一个实施方案中，本发明涉及一种生产糖基化的阿魏酸-2-硫酸酯酶的方法，其包括培养含有编码酶活性的阿魏酸-2-硫酸酯酶多肽的核酸的宿主细胞，其中宿主细胞将多肽糖基化成比由天然人肝细胞表达的天然异二烯酸2-硫酸酯酶多肽更大程度。

5. 发明授权

US5798239A Glycosylation variants of iduronate 2-sulfatase 失效
公开(公告)号：US5798239A
公开(公告)日：1998-08-25
申请号：US484494
申请日：1995-06-07
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46 , A61K38/47 , C12N9/00
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

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