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1. 发明授权

US06541254B1 Glycosylation variants of iduronate 2-sulfatase 有权
标题翻译：糖尿病糖苷酸2-硫酸酯酶的糖基化变体
公开(公告)号：US06541254B1
公开(公告)日：2003-04-01
申请号：US09685844
申请日：2000-10-10
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： C12N510
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
摘要翻译：本发明提供了高度糖基化的糖尿病2-硫酸酯酶，其包含与天然来源例如纯化的伊曲膦酸酯-2-硫酸酯酶相比，具有至少5千道尔顿（kDa）多糖的伊万二酸-2-硫酸酯酶多肽。人肝。本发明还提供了这样一种高度糖基化的阿魏酸-2-硫酸酯酶的酶活性多肽片段或变体。本发明还提供了编码艾杜糖醛酸-2-硫酸酯酶的分离的核酸，以及表达载体，宿主细胞和用于制备本发明的高度糖基化的阿拉伯糖酸-2-硫酸酯酶的方法。在一个实施方案中，本发明涉及一种生产糖基化的阿魏酸-2-硫酸酯酶的方法，其包括培养含有编码酶活性的阿魏酸-2-硫酸酯酶多肽的核酸的宿主细胞，其中宿主细胞将多肽糖基化成比由天然人肝细胞表达的天然异二烯酸2-硫酸酯酶多肽更大程度。

2. 发明授权

US06491913B2 Synthetic mammalian sulphamidase and genetic sequences encoding same 有权
标题翻译：合成哺乳动物磺酰胺酶和编码相同的遗传序列
公开(公告)号：US06491913B2
公开(公告)日：2002-12-10
申请号：US09729499
申请日：2000-12-04
申请人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
发明人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
IPC分类号： A61K3846
CPC分类号： C12N9/14 , A61K38/00 , Y02A50/395
摘要： The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.
摘要翻译：本发明一般涉及哺乳动物磺酰胺酶和编码它们的遗传序列，以及这些在用于疑似或患有硫胺酶缺乏症的受试者的调查，诊断和治疗中的用途。

3. 发明授权

US06200563B1 Synthetic mammalian sulphamidase and genetic sequences encoding same 有权
标题翻译：合成哺乳动物磺酰胺酶和编码相同的遗传序列
公开(公告)号：US06200563B1
公开(公告)日：2001-03-13
申请号：US09370867
申请日：1999-08-09
申请人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
发明人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
IPC分类号： A61K3846
CPC分类号： C12N9/14 , A61K38/00 , Y02A50/395
摘要： The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.
摘要翻译：本发明一般涉及哺乳动物磺酰胺酶和编码它们的遗传序列，以及这些在用于疑似或患有硫胺酶缺乏症的受试者的调查，诊断和治疗中的用途。

4. 发明授权

US5972333A Synthetic mammalian sulphamidase and genetic sequences encoding same 失效
公开(公告)号：US5972333A
公开(公告)日：1999-10-26
申请号：US874763
申请日：1997-06-13
申请人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
发明人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
IPC分类号： A61K38/00 , A61P3/00 , C12N9/14 , A61K38/47 , C07H21/04 , C07K1/00
CPC分类号： C12N9/14 , A61K38/00
摘要： The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.

5. 发明授权

US5849544A Amplification and detection process 失效
标题翻译：扩增和检测过程
公开(公告)号：US5849544A
公开(公告)日：1998-12-15
申请号：US374764
申请日：1995-01-24
申请人： Raymond John Harris , Charles Phillip Morris
发明人： Raymond John Harris , Charles Phillip Morris
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6834 , C12Q1/6816 , C12Q1/6844 , C12Q1/686
摘要： This method for detecting a target nucleic acid sequence involves amplification and detection in the same vessel and comprises: (a) amplification of the target nucleic sequence in a vessel which is provided with a solid phase capture probe comprising a nucleic acid sequence capable of hybridizing to at least a portion of said amplified target nucleic acid sequence, said capture probe being incapable of participating or not participating in standard nucleic acid sequence amplification processes, (b) bringing a sample suspected of comprising said target nucleic acid sequence into contact with said capture probe under conditions which allow said amplified target nucleic acid sequence to be bound by said capture probe, and (c) detecting the presence of bound target nucleic acid sequence. In a further aspect, the present invention provides an assay system or kit, for detecting a target nucleic acid sequence in a sample suspected of comprising said target nucleic acid sequence, comprising: (a) a capture probe comprising a nucleic acid sequence capable of hydridizing to at least a portion of said amplified target nucleic acid sequence, said capture probe being immobilized on a solid phase support which forms a part of or is insertable into a container for the sample, and said capture probe being incapable of participating in standard nucleic acid sequence amplification processes, (b) reagents for amplification of said target nucleic acid sequence, and (c) means for detecting said target nucleic acid sequence, when bound by said capture probe.
摘要翻译： PCT No.PCT / AU93 / 00379 Sec。 371日期1995年1月24日 102（e）1995年1月24日PCT PCT 1993年7月26日PCT公布。公开号WO94 / 02634 日本1994年2月3日该方法用于检测靶核酸序列涉及在同一容器中的扩增和检测，包括：（a）在提供有包含核酸的固相捕获探针的容器中扩增靶核酸序列能够与所述扩增的靶核酸序列的至少一部分杂交的序列，所述捕获探针不能参与或不参与标准核酸序列扩增过程，（b）将疑似含有所述靶核酸序列的样品置于在允许所述扩增的靶核酸序列被所述捕获探针结合的条件下与所述捕获探针接触，和（c）检测结合的靶核酸序列的存在。在另一方面，本发明提供了一种用于检测疑似含有所述靶核酸序列的样品中的靶核酸序列的测定系统或试剂盒，其包含：（a）包含能够进行杂交的核酸序列的捕获探针至少部分所述扩增的靶核酸序列，所述捕获探针固定在固相载体上，所述固相载体形成样品的一部分或可插入样品的容器中，并且所述捕获探针不能参与标准核酸序列扩增方法，（b）用于扩增所述靶核酸序列的试剂，和（c）用所述捕获探针结合时检测所述靶核酸序列的方法。

6. 发明授权

US06458579B2 Synthetic mammalian sulphamidase and genetic sequences encoding same 有权
公开(公告)号：US06458579B2
公开(公告)日：2002-10-01
申请号：US09729472
申请日：2000-12-04
申请人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
发明人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Lianne Cheryl Blanch , Xiao Hui Guo
IPC分类号： C12N120
CPC分类号： C12N9/14 , A61K38/00 , Y02A50/395
摘要： The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.

7. 发明授权

US06238662B1 Synthetic &agr;-L-iduronidase and genetic sequences encoding same 失效
标题翻译：合成的α-L-艾杜糖醛酸酶和编码相同的遗传序列
公开(公告)号：US06238662B1
公开(公告)日：2001-05-29
申请号：US09385707
申请日：1999-08-30
申请人： Hamish Steel Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
发明人： Hamish Steel Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
IPC分类号： A61K3847
CPC分类号： C12Y302/01076 , A61K38/00 , C07K2319/00 , C12N9/2402
摘要： The present invention relates generally to &agr;-L-iduronidase and to genetic sequences encoding same. More particularly, the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides which encodes or are complementary to a sequence which encodes a mammalian &agr;-L-iduronidase or fragment or derivative thereof and to the recombinant enzyme encoded thereby. These molecules are useful in the investigation, diagnosis and treatment of subjects suspected of or suffering from &agr;-L-iduronidase deficiency.
摘要翻译：本发明一般涉及α-L-艾杜糖醛酸酶和编码其的遗传序列。更具体地，本发明提供了分离的核酸分子，其包含编码或与编码哺乳动物α-L-艾杜糖醛酸酶或其片段或衍生物的序列和与其编码的重组酶的序列互补的核苷酸序列。这些分子可用于疑似或遭受α-L-艾杜糖醛酸酶缺乏症的受试者的研究，诊断和治疗。

8. 发明授权

US5863782A Synthetic mammalian sulphamidase and genetic sequences encoding same 失效
公开(公告)号：US5863782A
公开(公告)日：1999-01-26
申请号：US424881
申请日：1995-04-19
申请人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Liane Cheryl Blanch , Xiao-Nui Guo
发明人： John Joseph Hopwood , Hamish Steele Scott , Craig Geoffrey Freeman , Charles Phillip Morris , Liane Cheryl Blanch , Xiao-Nui Guo
IPC分类号： A61K38/00 , A61P3/00 , C12N9/14 , C12N15/63 , C12N5/10 , C07H21/04
CPC分类号： C12N9/14 , A61K38/00
摘要： The present invention relates generally to mammalian sulphamidase and to genetic sequences encoding same and to the use of these in the investigation, diagnosis and treatment of subjects suspected of or suffering from sulphamidase deficiency.

9. 发明授权

US5728381A Glycosylation variants of iduronate 2-sulfatase 失效
公开(公告)号：US5728381A
公开(公告)日：1998-03-17
申请号：US484493
申请日：1995-06-07
申请人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
发明人： Peter J. Wilson , Charles Phillip Morris , Donald Stewart Anson , Teresa Occhiodoro , Julie Bielicki , Peter Roy Clements , John Joseph Hopwood
IPC分类号： A61K38/00 , C12N5/18 , C12N5/22 , C12N9/16 , A61K38/46 , A61K38/47 , C12N9/00
CPC分类号： C12N9/16 , A61K38/00
摘要： The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.

10. 发明授权

US06524835B1 Synthetic &agr;-L-iduronidase and genetic sequences encoding same 失效
标题翻译：合成的α-L-艾杜糖醛酸酶和编码相同的遗传序列
公开(公告)号：US06524835B1
公开(公告)日：2003-02-25
申请号：US09639696
申请日：2000-08-16
申请人： Hamish Steel Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
发明人： Hamish Steel Scott , Donald Stewart Anson , Annette Marie Orsborn , Paul Victor Nelson , Peter Roy Clements , Charles Phillip Morris , John Joseph Hopwood
IPC分类号： C12N924
CPC分类号： C12Y302/01076 , A61K38/00 , C07K2319/00 , C12N9/2402
摘要： The present invention relates generally to &agr;-L-iduronidase and to genetic sequences encoding same. More particularly, the present invention provides an isolated nucleic acid molecule comprising a sequence of nucleotides which encodes or are complementary to a sequence which encodes a mammalian &agr;-L-iduronidase or fragment or derivative thereof and to the recombinant enzyme encoded thereby. These molecules are useful in the investigation, diagnosis and treatment of subjects suspected of or suffering from &agr;-L-iduronidase deficiency.
摘要翻译：本发明一般涉及α-L-艾杜糖醛酸酶和编码其的遗传序列。更具体地，本发明提供了分离的核酸分子，其包含编码或与编码哺乳动物α-L-艾杜糖醛酸酶或其片段或衍生物的序列和与其编码的重组酶的序列互补的核苷酸序列。这些分子可用于疑似或遭受α-L-艾杜糖醛酸酶缺乏症的受试者的调查，诊断和治疗。

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